SitesBLAST
Comparing WP_092053439.1 NCBI__GCF_900111775.1:WP_092053439.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3du4A Crystal structure of 7-keto-8-aminopelargonic acid bound 7,8- diaminopelargonic acid synthase in bacillus subtilis (see paper)
44% identity, 99% coverage: 5:448/449 of query aligns to 4:445/448 of 3du4A
- active site: F17 (≠ C18), Y146 (= Y148), E217 (= E220), D251 (= D254), A254 (= A257), K280 (= K283), A417 (≠ Y420)
- binding 7-keto-8-aminopelargonic acid: L82 (≠ A84), Y146 (= Y148), G315 (≠ S318), S317 (= S320), R410 (= R413)
- binding pyridoxal-5'-phosphate: S112 (≠ N114), G113 (= G115), A114 (≠ S116), Y146 (= Y148), H147 (= H149), E217 (= E220), D251 (= D254), V253 (≠ I256), A254 (= A257), K280 (= K283), H316 (= H319), S317 (= S320)
P53555 L-Lysine--8-amino-7-oxononanoate transaminase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; L-Lysine--8-amino-7-oxononanoate aminotransferase; EC 2.6.1.105 from Bacillus subtilis (strain 168) (see paper)
44% identity, 99% coverage: 5:448/449 of query aligns to 4:445/448 of P53555
- GA 113:114 (≠ GS 115:116) binding
- Y146 (= Y148) binding
- K280 (= K283) modified: N6-(pyridoxal phosphate)lysine
- G315 (≠ S318) binding
- HS 316:317 (= HS 319:320) binding
- R410 (= R413) binding
6wnnA Bacillus subtilis bioa in complex with amino donor l-lys
41% identity, 99% coverage: 5:448/449 of query aligns to 1:417/420 of 6wnnA
- active site: F14 (≠ C18), Y143 (= Y148), D223 (= D254), K252 (= K283)
- binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: F14 (≠ C18), W51 (= W56), S109 (≠ N114), G110 (= G115), A111 (≠ S116), Y143 (= Y148), H144 (= H149), D223 (= D254), V225 (≠ I256), K252 (= K283), R382 (= R413)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: H288 (= H319), S289 (= S320)
3dodA Crystal structure of plp bound 7,8-diaminopelargonic acid synthase in bacillus subtilis (see paper)
41% identity, 99% coverage: 5:448/449 of query aligns to 2:414/417 of 3dodA
- active site: F15 (≠ C18), E186 (= E220), D220 (= D254), A223 (= A257), K249 (= K283), A386 (≠ Y420)
- binding pyridoxal-5'-phosphate: W52 (= W56), S110 (≠ N114), G111 (= G115), A112 (≠ S116), D220 (= D254), V222 (≠ I256), K249 (= K283), H285 (= H319), S286 (= S320)
6zhkA Crystal structure of adenosylmethionine-8-amino-7-oxononanoate aminotransferase from methanocaldococcus jannaschii dsm 2661
38% identity, 98% coverage: 8:445/449 of query aligns to 7:435/438 of 6zhkA
6erkA Crystal structure of diaminopelargonic acid aminotransferase from psychrobacter cryohalolentis (see paper)
39% identity, 97% coverage: 1:435/449 of query aligns to 1:405/420 of 6erkA
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
38% identity, 100% coverage: 1:447/449 of query aligns to 1:425/429 of P12995
- Y17 (≠ C18) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W55) binding
- GS 112:113 (= GS 115:116) binding
- Y144 (= Y148) mutation to F: Severely reduces the aminotransferase activity.
- D147 (≠ E151) mutation to N: Loss of aminotransferase activity.
- D245 (= D254) binding
- R253 (= R262) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K283) binding ; modified: N6-(pyridoxal phosphate)lysine
- G307 (≠ S318) binding
- PT 308:309 (≠ HS 319:320) binding
- R391 (= R413) binding ; mutation to A: Reduces aminotransferase activity.
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
38% identity, 98% coverage: 7:447/449 of query aligns to 6:425/429 of 6ed7A
- active site: Y17 (≠ C18), Y144 (= Y148), D245 (= D254), K274 (= K283)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (≠ C18), W52 (= W55), W52 (= W55), Y144 (= Y148), D147 (≠ E151), A217 (= A226), K274 (= K283), R391 (= R413), F393 (≠ L415), F393 (≠ L415)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y148), H145 (= H149), D245 (= D254), I247 (= I256), K274 (= K283)
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
38% identity, 100% coverage: 1:447/449 of query aligns to 1:425/429 of 1dtyA
- active site: Y17 (≠ C18), Y144 (= Y148), E211 (= E220), D245 (= D254), A248 (= A257), K274 (= K283), Y398 (= Y420)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y148), H145 (= H149), D245 (= D254), I247 (= I256), K274 (= K283)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
38% identity, 100% coverage: 1:447/449 of query aligns to 1:424/427 of 1mlzA
- active site: Y17 (≠ C18), Y144 (= Y148), E210 (= E220), D244 (= D254), A247 (= A257), K273 (= K283), Y397 (= Y420)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y148), H145 (= H149), D244 (= D254), I246 (= I256), K273 (= K283), P307 (≠ H319), T308 (≠ S320)
- binding trans-amiclenomycin: W52 (= W55), W53 (= W56), Y144 (= Y148), K273 (= K283), R390 (= R413), F392 (≠ L415)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
38% identity, 100% coverage: 1:447/449 of query aligns to 1:424/427 of 1mlyA
- active site: Y17 (≠ C18), Y144 (= Y148), E210 (= E220), D244 (= D254), A247 (= A257), K273 (= K283), Y397 (= Y420)
- binding cis-amiclenomycin: W52 (= W55), W53 (= W56), K273 (= K283), R390 (= R413), F392 (≠ L415)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y148), H145 (= H149), D244 (= D254), I246 (= I256), K273 (= K283), P307 (≠ H319), T308 (≠ S320)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
37% identity, 99% coverage: 1:446/449 of query aligns to 1:421/427 of 4cxqA
- active site: Y18 (≠ C18), Y149 (= Y148), E212 (= E220), D246 (= D254), A249 (= A257), K275 (= K283), Y399 (= Y420)
- binding 7-keto-8-aminopelargonic acid: W56 (= W55), Y149 (= Y148), G308 (≠ S318), T310 (≠ S320), R392 (= R413)
- binding pyridoxal-5'-phosphate: G116 (= G115), S117 (= S116), Y149 (= Y148), H150 (= H149), G151 (= G150), E212 (= E220), D246 (= D254), I248 (= I256), K275 (= K283), P309 (≠ H319), T310 (≠ S320)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
36% identity, 99% coverage: 1:446/449 of query aligns to 1:419/425 of 4w1vA
- active site: Y18 (≠ C18), Y147 (= Y148), E210 (= E220), D244 (= D254), A247 (= A257), K273 (= K283), Y397 (= Y420)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P17), Y18 (≠ C18), W54 (= W55), M81 (≠ I82), G83 (≠ A84), Y147 (= Y148), G306 (≠ S318), P307 (≠ H319), T308 (≠ S320), F392 (≠ L415)
- binding pyridoxal-5'-phosphate: G114 (= G115), S115 (= S116), Y147 (= Y148), H148 (= H149), E210 (= E220), D244 (= D254), I246 (= I256), K273 (= K283)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
36% identity, 99% coverage: 1:446/449 of query aligns to 1:419/425 of 4cxrA
- active site: Y18 (≠ C18), Y147 (= Y148), E210 (= E220), D244 (= D254), A247 (= A257), K273 (= K283), Y397 (= Y420)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (≠ C18), W54 (= W55), W55 (= W56), A216 (= A226)
- binding pyridoxal-5'-phosphate: G114 (= G115), S115 (= S116), Y147 (= Y148), H148 (= H149), E210 (= E220), D244 (= D254), I246 (= I256), K273 (= K283), P307 (≠ H319), T308 (≠ S320)
6ge8A Crystal structure of mycobacterium tuberculosis bioa
37% identity, 99% coverage: 1:446/449 of query aligns to 2:417/422 of 6ge8A
- binding [(2~{S})-3-(1~{H}-indol-3-yl)-1-[(2~{E})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylidene]hydrazinyl]-1-oxidanylidene-propan-2-yl]azanium: Y19 (≠ C18), W58 (= W55), G118 (= G115), S119 (= S116), Y151 (= Y148), H152 (= H149), E208 (= E220), D242 (= D254), I244 (= I256), K271 (= K283), P305 (≠ H319), T306 (≠ S320)
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
37% identity, 99% coverage: 1:446/449 of query aligns to 1:422/428 of 5te2A
- active site: Y18 (≠ C18), Y150 (= Y148), E213 (= E220), D247 (= D254), A250 (= A257), K276 (= K283), Y400 (= Y420)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-4-oxo-1,4-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: Y18 (≠ C18), W57 (= W55), G117 (= G115), S118 (= S116), Y150 (= Y148), H151 (= H149), G152 (= G150), D247 (= D254), I249 (= I256), K276 (= K283), G309 (≠ S318), P310 (≠ H319), T311 (≠ S320)
4xjoA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
37% identity, 99% coverage: 1:446/449 of query aligns to 1:422/428 of 4xjoA
- active site: Y18 (≠ C18), Y150 (= Y148), E213 (= E220), D247 (= D254), A250 (= A257), K276 (= K283), Y400 (= Y420)
- binding 5-[4-(3-chlorobenzoyl)piperazin-1-yl]-1H-inden-1-one: P17 (= P17), Y18 (≠ C18), W57 (= W55), M84 (≠ I82), G86 (≠ A84), Y150 (= Y148), G165 (vs. gap), G166 (= G159), A219 (= A226), P310 (≠ H319), T311 (≠ S320)
- binding pyridoxal-5'-phosphate: G117 (= G115), S118 (= S116), Y150 (= Y148), H151 (= H149), G152 (= G150), E213 (= E220), D247 (= D254), I249 (= I256), K276 (= K283), P310 (≠ H319), T311 (≠ S320)
4xewA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound (see paper)
37% identity, 99% coverage: 1:446/449 of query aligns to 1:422/428 of 4xewA
- active site: Y18 (≠ C18), Y150 (= Y148), E213 (= E220), D247 (= D254), A250 (= A257), K276 (= K283), Y400 (= Y420)
- binding 6-(2-fluorophenyl)[1,3]dioxolo[4,5-g]quinolin-8(5H)-one: P17 (= P17), Y18 (≠ C18), W57 (= W55), Y150 (= Y148), P310 (≠ H319), T311 (≠ S320), R393 (= R413), F395 (≠ L415)
- binding pyridoxal-5'-phosphate: G117 (= G115), S118 (= S116), Y150 (= Y148), H151 (= H149), G152 (= G150), E213 (= E220), D247 (= D254), I249 (= I256), K276 (= K283), P310 (≠ H319), T311 (≠ S320)
4wyfA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a dsf fragment hit (see paper)
37% identity, 99% coverage: 1:446/449 of query aligns to 1:422/428 of 4wyfA
- active site: Y18 (≠ C18), Y150 (= Y148), E213 (= E220), D247 (= D254), A250 (= A257), K276 (= K283), Y400 (= Y420)
- binding N-(1-oxo-1H-inden-5-yl)acetamide: M84 (≠ I82), G86 (≠ A84), G309 (≠ S318), P310 (≠ H319), T311 (≠ S320)
- binding pyridoxal-5'-phosphate: G117 (= G115), S118 (= S116), Y150 (= Y148), H151 (= H149), G152 (= G150), E213 (= E220), D247 (= D254), I249 (= I256), K276 (= K283), P310 (≠ H319), T311 (≠ S320)
4wycA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole benzamide inhibitor (see paper)
37% identity, 99% coverage: 1:446/449 of query aligns to 1:422/428 of 4wycA
- active site: Y18 (≠ C18), Y150 (= Y148), E213 (= E220), D247 (= D254), A250 (= A257), K276 (= K283), Y400 (= Y420)
- binding 4-(1H-imidazol-1-yl)benzamide: P17 (= P17), Y18 (≠ C18), W57 (= W55), M84 (≠ I82), G86 (≠ A84), Y150 (= Y148), G309 (≠ S318)
- binding pyridoxal-5'-phosphate: G117 (= G115), S118 (= S116), Y150 (= Y148), H151 (= H149), G152 (= G150), E213 (= E220), D247 (= D254), I249 (= I256), K276 (= K283), P310 (≠ H319), T311 (≠ S320)
Query Sequence
>WP_092053439.1 NCBI__GCF_900111775.1:WP_092053439.1
MTKEEILRLDRRHVWHPCTQEKDHEALPPIPIARGDGAYLYDFDGKGYIDGVSSWWVNLF
GHNHPRLNRALSKQAGKVAHHIFAGFTHEPAATLAARLCELAPPGLEKVFFTDNGSAAVE
AALKMSFQYWRQTGQGEKTRFVSITEAYHGETLGALSVGGCGLYKDIYQPLLLDGFQVQG
PDCFRCPYGCERESCNAECFAAMEEVVAEHHRQIAAVIIEPLIQCAAGMRIYPPIYLKKL
RELCDRWRIHYIADEIAVGFGRTGRLFANEHAGVSPDLLCLSKGITGGYMPLSVVLTRQE
MYDAFYDDYATLKAFLHSHSYSGNPLACALACEVLNIFTEEKILEKMVPKMAILASQKHR
FEALPQVGEFRQLGLVGAIELVRDKVGKTPYPWQERRGYAVYRKALEKGALLRPLGNVVY
FMPPLTIEKADLSRLVDIAYEAIVEVTAD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory