SitesBLAST
Comparing WP_092057925.1 NCBI__GCF_900111775.1:WP_092057925.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1g291 Malk (see paper)
37% identity, 68% coverage: 15:262/366 of query aligns to 21:269/372 of 1g291
- binding magnesium ion: D69 (= D62), E71 (= E64), K72 (≠ R65), K79 (≠ R71), D80 (≠ L72)
- binding pyrophosphate 2-: S38 (= S32), G39 (= G33), C40 (≠ S34), G41 (= G35), K42 (= K36), T43 (= T37), T44 (≠ S38)
Sites not aligning to the query:
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
31% identity, 93% coverage: 15:355/366 of query aligns to 24:363/375 of 2d62A
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 63% coverage: 12:241/366 of query aligns to 34:259/378 of P69874
- F45 (≠ I25) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ S34) mutation to T: Loss of ATPase activity and transport.
- L60 (= L40) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L56) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ A117) mutation to M: Loss of ATPase activity and transport.
- D172 (= D154) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 26 C→A: Lower ATPase activity and transport efficiency.
- 27 F→L: Lower ATPase activity and transport efficiency.
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
38% identity, 58% coverage: 13:223/366 of query aligns to 42:256/382 of 7ahhC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
- binding phosphoaminophosphonic acid-adenylate ester: 12, 39, 40, 41
7aheC Opua inhibited inward facing (see paper)
38% identity, 58% coverage: 13:223/366 of query aligns to 42:256/382 of 7aheC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
34% identity, 86% coverage: 20:332/366 of query aligns to 29:326/353 of 1vciA
Sites not aligning to the query:
7ahdC Opua (e190q) occluded (see paper)
37% identity, 58% coverage: 13:223/366 of query aligns to 42:256/260 of 7ahdC
- binding adenosine-5'-triphosphate: S61 (= S32), G62 (= G33), G64 (= G35), K65 (= K36), S66 (≠ T37), T67 (≠ S38), Q111 (= Q80), K161 (≠ H128), Q162 (≠ E129), S164 (= S131), G166 (= G133), M167 (≠ Q134), Q188 (≠ E155), H221 (= H188)
Sites not aligning to the query:
3d31A Modbc from methanosarcina acetivorans (see paper)
33% identity, 89% coverage: 15:339/366 of query aligns to 18:331/348 of 3d31A
Sites not aligning to the query:
8hprC Lpqy-sugabc in state 4 (see paper)
35% identity, 63% coverage: 15:245/366 of query aligns to 21:238/363 of 8hprC
- binding adenosine-5'-triphosphate: S38 (= S32), G39 (= G33), G41 (= G35), K42 (= K36), S43 (≠ T37), Q82 (= Q80), Q133 (≠ E129), G136 (= G132), G137 (= G133), Q138 (= Q134), H192 (= H188)
- binding magnesium ion: S43 (≠ T37), Q82 (= Q80)
Sites not aligning to the query:
8hplC Lpqy-sugabc in state 1 (see paper)
35% identity, 63% coverage: 15:245/366 of query aligns to 19:236/384 of 8hplC
Sites not aligning to the query:
8hprD Lpqy-sugabc in state 4 (see paper)
35% identity, 63% coverage: 15:245/366 of query aligns to 21:238/362 of 8hprD
- binding adenosine-5'-triphosphate: S38 (= S32), C40 (≠ S34), G41 (= G35), K42 (= K36), S43 (≠ T37), T44 (≠ S38), Q82 (= Q80), R129 (= R125), Q133 (≠ E129), S135 (= S131), G136 (= G132), G137 (= G133), Q159 (≠ E155), H192 (= H188)
- binding magnesium ion: S43 (≠ T37), Q82 (= Q80)
Sites not aligning to the query:
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 87% coverage: 15:331/366 of query aligns to 21:319/369 of P19566
- L86 (= L84) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P156) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D161) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ P318) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 93% coverage: 15:356/366 of query aligns to 20:334/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: S37 (= S32), G38 (= G33), C39 (≠ S34), G40 (= G35), K41 (= K36), S42 (≠ T37), T43 (≠ S38), Q81 (= Q80), R128 (= R125), A132 (≠ E129), S134 (= S131), G136 (= G133), Q137 (= Q134), E158 (= E155), H191 (= H188)
- binding magnesium ion: S42 (≠ T37), Q81 (= Q80)
Sites not aligning to the query:
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 93% coverage: 15:356/366 of query aligns to 20:334/371 of 3puxA
- binding adenosine-5'-diphosphate: G38 (= G33), C39 (≠ S34), G40 (= G35), K41 (= K36), S42 (≠ T37), T43 (≠ S38), R128 (= R125), S134 (= S131), Q137 (= Q134)
- binding beryllium trifluoride ion: S37 (= S32), G38 (= G33), K41 (= K36), Q81 (= Q80), S134 (= S131), G136 (= G133), H191 (= H188)
- binding magnesium ion: S42 (≠ T37), Q81 (= Q80)
Sites not aligning to the query:
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 93% coverage: 15:356/366 of query aligns to 20:334/371 of 3puwA
- binding adenosine-5'-diphosphate: G38 (= G33), C39 (≠ S34), G40 (= G35), K41 (= K36), S42 (≠ T37), T43 (≠ S38), R128 (= R125), A132 (≠ E129), S134 (= S131), Q137 (= Q134)
- binding tetrafluoroaluminate ion: S37 (= S32), G38 (= G33), K41 (= K36), Q81 (= Q80), S134 (= S131), G135 (= G132), G136 (= G133), E158 (= E155), H191 (= H188)
- binding magnesium ion: S42 (≠ T37), Q81 (= Q80)
Sites not aligning to the query:
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
31% identity, 93% coverage: 15:356/366 of query aligns to 20:334/371 of 3puvA
- binding adenosine-5'-diphosphate: G38 (= G33), C39 (≠ S34), G40 (= G35), K41 (= K36), S42 (≠ T37), T43 (≠ S38), R128 (= R125), A132 (≠ E129), S134 (= S131), Q137 (= Q134)
- binding magnesium ion: S42 (≠ T37), Q81 (= Q80)
Sites not aligning to the query:
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
32% identity, 89% coverage: 15:338/366 of query aligns to 18:323/367 of 1q12A
- binding adenosine-5'-triphosphate: S35 (= S32), G36 (= G33), C37 (≠ S34), G38 (= G35), K39 (= K36), S40 (≠ T37), T41 (≠ S38), R126 (= R125), A130 (≠ E129), S132 (= S131), G134 (= G133), Q135 (= Q134)
Sites not aligning to the query:
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 89% coverage: 15:338/366 of query aligns to 21:326/371 of P68187
- A85 (≠ G83) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P102) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A110) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ Y113) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A115) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E120) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G133) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D154) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ V235) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ W253) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (= W274) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (≠ R285) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ P289) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (= G291) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G310) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ P318) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (= S334) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
32% identity, 89% coverage: 15:338/366 of query aligns to 20:325/374 of 2awnB
Sites not aligning to the query:
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 68% coverage: 19:268/366 of query aligns to 27:273/353 of 1oxvD
Sites not aligning to the query:
Query Sequence
>WP_092057925.1 NCBI__GCF_900111775.1:WP_092057925.1
MNLSVAIRHEGPLLDFAAELPEGRITALVGPSGSGKTSILHAIAGLLRVRQARIRLGEAV
WDDERVHLPTRLRPIGLVSQHYGLFPHLTAQANVEMSLTHLPKGQRRERARHYLALARVE
GLEGRFPHELSGGQRQRVALARAIARDPQLLLLDEPFSAVDRSTRQRLYIELRRLHAELR
TTILLVTHDLDEAARMADHLVLLRHGRLLQAGPTAAVLARPASVSAARLLDIPNVFAGEF
IGTDAEGAALLNWGAHRLRLTPAPDVAVGTTVRWAVLPSNLLLVRPDKPWGEHLENPIPG
RVCEVLELGGEALVWVVPDGLPETRMQMRIPVRSLRRSPLQVGDPLTLCLRAADIVPLAD
APAHEP
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory