SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_092058696.1 NCBI__GCF_900111775.1:WP_092058696.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
22% identity, 32% coverage: 416:625/657 of query aligns to 2:234/291 of 5odqB

query
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5odqB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G422), C9 (= C423), C41 (≠ L457), C42 (= C458), C78 (= C495), G80 (≠ H497), C81 (= C498), G152 (≠ D546), C153 (≠ S547), H154 (≠ C548), C193 (= C584), C194 (= C585), C231 (= C622), F233 (≠ Y624), C234 (= C625)
binding 2-bromanylethanesulfonic acid: G46 (≠ S462), G80 (≠ H497), H154 (≠ C548), G197 (= G588), R201 (≠ M592), F233 (≠ Y624)

Sites not aligning to the query:

binding 2-bromanylethanesulfonic acid: 236

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
22% identity, 32% coverage: 416:625/657 of query aligns to 2:234/291 of 5odhH

query
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5odhH

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G422), C9 (= C423), C41 (≠ L457), C42 (= C458), C78 (= C495), G80 (≠ H497), C81 (= C498), C153 (≠ S547), H154 (≠ C548), C193 (= C584), C194 (= C585), C231 (= C622), F233 (≠ Y624), C234 (= C625)
binding 1-thioethanesulfonic acid: A44 (≠ G460), P45 (≠ D461), G46 (≠ S462), G80 (≠ H497), H154 (≠ C548), G197 (= G588), G198 (= G589)
binding Coenzyme B: R201 (≠ M592), F233 (≠ Y624)

Sites not aligning to the query:

binding Coenzyme B: 240

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
22% identity, 32% coverage: 416:625/657 of query aligns to 2:234/291 of 5odhB

query
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5odhB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G422), C9 (= C423), I10 (≠ A424), C41 (≠ L457), C42 (= C458), C78 (= C495), G80 (≠ H497), C81 (= C498), G152 (≠ D546), C153 (≠ S547), H154 (≠ C548), C193 (= C584), C194 (= C585), C231 (= C622), F233 (≠ Y624), C234 (= C625)
binding 1-thioethanesulfonic acid: P45 (≠ D461), G46 (≠ S462), G80 (≠ H497), F233 (≠ Y624)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
22% identity, 32% coverage: 416:625/657 of query aligns to 2:234/291 of 5odcB

query
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5odcB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G422), C9 (= C423), I10 (≠ A424), C41 (≠ L457), C42 (= C458), C78 (= C495), N79 (≠ P496), G80 (≠ H497), C81 (= C498), G152 (≠ D546), C153 (≠ S547), C193 (= C584), C194 (= C585), G197 (= G588), C231 (= C622), F233 (≠ Y624), C234 (= C625)

Query Sequence

>WP_092058696.1 NCBI__GCF_900111775.1:WP_092058696.1
MTFSGFIVLLLFGASGGFFLWSLYQRLGLVRLGRDENRFDRVDERLRSVLTYVFGQKRVL
ARPFGINHAIFFWACVLLVAVNVEFVLGGIFPGARLSVLPGLFYFPIRFVSDVFSLLTLC
AVLAALIHRTFFPLYPEARNFEGYFIVAVIAVHMLAYFGISGAEVALGHERAAGWMPVSA
AFAQAFSGFGEVGLERIHGFFWLVHALALLILFNYLIPYSKHLHVFTAIGNCFFRSLEKP
NTQPRETFALDLPLGADRPTRMTWKDLFDGFACTECGRCEDVCPAHNTGKKLNPRQVVHD
LKVNLLENGPALKGGGEPRLPLIGEVGEGSCSEEAIWDCTTCGACLEACPVFIEHPQKLV
KMRRHLVQMEAKFPDELLNLFENMEQRSNPWGMAPAERGKWAALLDPQEFAADKTEYLFF
VGCAGAFDTRNKHVTVAVATILNAAGVSWGILGKDELCCGDSVRRLGNEFVFERMALKNV
ELLQARGVKKVITQCPHCFSALKNDYRQFGLELEVVHHSQLIAQLIADGRLKLPKPMNFG
RTIFHDSCYLGRHNDTYQAPRQLIEAATGFPPAEFERKRENSFCCGAGGGRMWMEEHTGE
RINRERVKEALTVAPDTLCVSCPYCMTMLVDGLKDEKAENVQVKDVAELVAEVLEQG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory