SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_092344816.1 NCBI__GCF_900107645.1:WP_092344816.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
24% identity, 31% coverage: 2:199/641 of query aligns to 23:221/662 of P11170

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P11170

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Sites not aligning to the query:

255 modified: Disulfide link with 608
457 Q→W: Drasticly decreased affinity for glucose and phlorizin.
460 T→W: Decreased affinity for glucose and phlorizin.
608 modified: Disulfide link with 255

Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
23% identity, 31% coverage: 2:198/641 of query aligns to 23:220/659 of Q9NY91

query
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Sites not aligning to the query:

457 E→Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.

Query Sequence

>WP_092344816.1 NCBI__GCF_900107645.1:WP_092344816.1
MIYTQSPLAIGLFIFFVLFVLGLSFYLSRRASSAEGYYAAGGNIHWATNGIAFAGDYLSA
ASFLGICGMIATAGYDGWMYSIGYLAGWIVALFLVAEPMKRLGKYTFTDALDSKFNSKSI
QLMAAISTLLVSVFYLIPQMVGAGVLVQPLLGLPHWVGVCIVGVVVTLIVATAGMASTTY
VQFFKGGMLLIMSTVLVGLVLFRGLSTTPADNNGTSYHDFKILHADSSMVIKEAGYSVIA
GTNSEFGKQGFVKLTKDGLESIWKIAESGTGYTLEETLFVTKMADGTKLFNGATAEEGKF
FPIGHIKELQINGEEVAATGAVGPLAFLSAIKDSTIVLWGKKYIKVGDETTTVYYQKPTP
GARILRPGLKFKVDNATGVQRFNFISLMLALFCGTAALPHILIRYYTVPSQAAARKSTIV
AIAAIGFFYVLTLFMGMGAMTSGVINITDNNMSAPLLALSFGFVMFSVISSLAFATVLGT
VSGLIVAASGAIAHDLMDNFLGMKMSDHGKVRAGKISAVIIGCIAIYLGIVFEGMNVSFL
VGWAFAVAASANLPAILMLLFWKRTTAKGIAASIFIGLTSSLGLILVSPDMWVRYGNLPS
EAPVQFNSPALISIPLSFLALVIVSMMTQKDVIIGHGTEVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory