SitesBLAST
Comparing WP_092344832.1 NCBI__GCF_900107645.1:WP_092344832.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7omlA Bacillus subtilis phosphoglucomutase glmm (metal bound) (see paper)
50% identity, 99% coverage: 4:451/452 of query aligns to 2:445/445 of 7omlA
7ojrA Bacillus subtilis phosphoglucomutase glmm (phosphate bound) (see paper)
50% identity, 99% coverage: 4:451/452 of query aligns to 2:445/445 of 7ojrA
P31120 Phosphoglucosamine mutase; EC 5.4.2.10 from Escherichia coli (strain K12) (see 3 papers)
49% identity, 99% coverage: 2:447/452 of query aligns to 3:443/445 of P31120
- S100 (= S101) mutation to A: 2% of wild-type activity.; mutation to T: 20-fold increase in the non-specific phosphoglucomutase activity towards glucose-phosphate substrates (non aminated).
- S102 (= S103) active site, Phosphoserine intermediate; modified: Phosphoserine; by autocatalysis; mutation to A: Loss of activity in the absence or presence of glucosamine-1,6-diP.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3i3wA Structure of a phosphoglucosamine mutase from francisella tularensis
40% identity, 99% coverage: 4:450/452 of query aligns to 1:440/441 of 3i3wA
- active site: R9 (= R12), S99 (= S103), H100 (= H104), K109 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding zinc ion: S99 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
35% identity, 96% coverage: 4:436/452 of query aligns to 3:440/455 of 1wqaA
- active site: R11 (= R12), S101 (= S103), H102 (= H104), K111 (= K113), D243 (= D245), D245 (= D247), D247 (= D249), R248 (= R250), G330 (≠ H334), R340 (≠ G344)
- binding magnesium ion: S101 (= S103), D243 (= D245), D245 (= D247), D247 (= D249)
6nqhA Xanthomonas citri dephospho-pgm in complex with xylose-1-phosphate
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6nqhA
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding magnesium ion: D237 (= D245), D239 (= D247), D241 (= D249)
- binding 1-O-phosphono-alpha-D-xylopyranose: S97 (= S103), H98 (= H104), K107 (= K113), D239 (= D247), R242 (= R250), R280 (≠ V289), S301 (≠ V311), G302 (= G312), E320 (= E330), S322 (= S332), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), T418 (= T419), R423 (= R424)
Sites not aligning to the query:
6np8A Xanthomonas citri phospho-pgm in complex with mannose-6-phosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6np8A
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding calcium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
- binding 6-O-phosphono-alpha-D-mannopyranose: R280 (≠ V289), G302 (= G312), H303 (≠ D313), E320 (= E330), S322 (= S332), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), T418 (= T419), R423 (= R424)
Sites not aligning to the query:
6nolA Xanthomonas citri dephospho-pgm in complex with mannose-1-phosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6nolA
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding 1-O-phosphono-alpha-D-mannopyranose: G302 (= G312), E320 (= E330), S322 (= S332), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), T418 (= T419), R423 (= R424)
- binding magnesium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
Sites not aligning to the query:
6nnpA Xanthomonas citri dephospho-pgm in complex with glucose-6-phosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6nnpA
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding 6-O-phosphono-alpha-D-glucopyranose: R280 (≠ V289), G302 (= G312), H303 (≠ D313), E320 (= E330), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), T418 (= T419), R423 (= R424)
- binding magnesium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
Sites not aligning to the query:
6nn2A Xanthomonas citri pgm apo-phospho (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6nn2A
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding calcium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
Sites not aligning to the query:
6n1eA Crystal structure of x. Citri phosphoglucomutase in complex with 1- methyl-glucose 6-phosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6n1eA
6mnvA Crystal structure of x. Citri phosphoglucomutase in complex with ch2fg1p (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6mnvA
- binding 1-deoxy-1-fluoro-2-O-phosphono-alpha-D-gluco-hept-2-ulopyranose: R280 (≠ V289), G302 (= G312), E320 (= E330), S322 (= S332), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), T418 (= T419), R423 (= R424)
- binding magnesium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
6mlhA Crystal structure of x. Citri phosphoglucomutase in complex with glucopyranosyl-1-methyl-phosphonic acid (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6mlhA
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding (1S)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: R280 (≠ V289), G302 (= G312), E320 (= E330), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), T418 (= T419), R423 (= R424)
- binding magnesium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
Sites not aligning to the query:
6mlfA Crystal structure of x. Citri phosphoglucomutase in complex with 6- fluoro glucose 1-phosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 6mlfA
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding 6-deoxy-6-fluoro-1-O-phosphono-alpha-D-glucopyranose: R280 (≠ V289), G302 (= G312), E320 (= E330), H324 (= H334), R414 (= R415), S416 (= S417), T418 (= T419), R423 (= R424)
- binding magnesium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
Sites not aligning to the query:
5kl0A Crystal structure of phosphoglucomutase from xanthomonas citri citri complexed with glucose-1,6-biphosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 17:427/448 of 5kl0A
- active site: S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D245), D239 (= D247), D241 (= D249), R242 (= R250), H324 (= H334)
- binding 1,6-di-O-phosphono-alpha-D-glucopyranose: S97 (= S103), G302 (= G312), H303 (≠ D313), E320 (= E330), H324 (= H334), R414 (= R415), S416 (= S417), N417 (≠ G418), R423 (= R424)
- binding magnesium ion: S97 (= S103), D237 (= D245), D239 (= D247), D241 (= D249)
Sites not aligning to the query:
6mlwA Crystal structure of x. Citri phosphoglucomutase in complex with 2- fluoro mannosyl-1-methyl-phosphonic acid (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 18:428/449 of 6mlwA
- active site: S98 (= S103), H99 (= H104), K108 (= K113), D238 (= D245), D240 (= D247), D242 (= D249), R243 (= R250), H325 (= H334)
- binding 2,6-anhydro-5,7-dideoxy-5-fluoro-7-phosphono-D-glycero-D-manno-heptitol: G303 (= G312), H304 (≠ D313), E321 (= E330), S323 (= S332), H325 (= H334), R415 (= R415), S417 (= S417), N418 (≠ G418), T419 (= T419), R424 (= R424)
- binding magnesium ion: S98 (= S103), D238 (= D245), D240 (= D247), D242 (= D249)
Sites not aligning to the query:
5bmpA Crystal structure of phosphoglucomutase from xanthomonas citri complexed with glucose-1-phosphate (see paper)
27% identity, 91% coverage: 18:428/452 of query aligns to 18:428/449 of 5bmpA
- active site: S98 (= S103), H99 (= H104), K108 (= K113), D238 (= D245), D240 (= D247), D242 (= D249), R243 (= R250), H325 (= H334)
- binding 1-O-phosphono-alpha-D-glucopyranose: R281 (≠ V289), G303 (= G312), E321 (= E330), S323 (= S332), H325 (= H334), R415 (= R415), S417 (= S417), N418 (≠ G418), T419 (= T419), R424 (= R424)
- binding magnesium ion: S98 (= S103), D238 (= D245), D240 (= D247), D242 (= D249)
Sites not aligning to the query:
2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
27% identity, 97% coverage: 1:438/452 of query aligns to 1:436/455 of 2h5aX
- active site: H101 (= H104), D234 (= D245), D236 (= D247), D238 (= D249), R239 (= R250), D332 (≠ G344)
- binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (≠ D9), T298 (≠ V311), G299 (= G312), H300 (≠ D313), E317 (= E330), S319 (= S332), H321 (= H334), R413 (= R415), S415 (= S417), N416 (≠ G418), T417 (= T419)
- binding zinc ion: S100 (= S103), D234 (= D245), D236 (= D247), D238 (= D249)
2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
27% identity, 97% coverage: 1:438/452 of query aligns to 1:436/455 of 2h4lX
- active site: H101 (= H104), D234 (= D245), D236 (= D247), D238 (= D249), R239 (= R250), D332 (≠ G344)
- binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (≠ D9), R12 (= R12), S100 (= S103), T298 (≠ V311), E317 (= E330), R413 (= R415), S415 (= S417), N416 (≠ G418), T417 (= T419)
- binding zinc ion: S100 (= S103), D234 (= D245), D236 (= D247), D238 (= D249)
2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
27% identity, 97% coverage: 1:438/452 of query aligns to 1:436/455 of 2fkfA
- active site: R12 (= R12), S100 (= S103), H101 (= H104), K110 (= K113), D234 (= D245), D236 (= D247), D238 (= D249), R239 (= R250), H321 (= H334), D332 (≠ G344)
- binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (≠ G7), H101 (= H104), S319 (= S332), R413 (= R415), S415 (= S417), N416 (≠ G418), T417 (= T419)
- binding zinc ion: S100 (= S103), D234 (= D245), D236 (= D247), D238 (= D249)
Query Sequence
>WP_092344832.1 NCBI__GCF_900107645.1:WP_092344832.1
MDKKLFGTDGVRGVANIDPMTTEMAMQLGRAAAFVFKNGGKRRHRVVIGKDTRLSGYMIE
NALVAGICSMGVDVLIVGPLPTPGIAFLTSSMRADAGVVISASHNPYQDNGIKFFSSTGY
KLQDELELKIEDLIINHRLDELRPIADEVGKAYRISDAIGRYIVFLKNTFPKDLDLEGVR
IVLDCAHGAGYKVAPAVLTELGAEVIPLGVNPNGMNINDGCGSMHPEVMAEKVCEYRADL
GIALDGDADRVIFVDEKGRTVDGDHIMALCGTEMIKAGKLKNKTVVATVMSNMGLEIAIK
KAGGQLVRTAVGDRYVVEEMLKEGYNLGGEQSGHMIFLDHNTTGDGILSALQVLAIIKRS
GEKLSELAKVMTSLPQVLVNVKVRKKAELRKIKPIKEVIDTIEAELGESGRLLVRYSGTE
ALLRVMIEGENQSQIESLAHKLAAVVQEHLGA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory