SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_092347989.1 NCBI__GCF_900107645.1:WP_092347989.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
60% identity, 100% coverage: 2:396/396 of query aligns to 1:394/394 of 1phpA

query
sites
1phpA

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active site: R36 (= R38), K197 (= K200), G351 (= G353), G374 (= G376)
binding adenosine-5'-diphosphate: G195 (= G198), K201 (= K204), G219 (= G222), G220 (= G223), L237 (= L240), N316 (= N319), P318 (= P321), G320 (= G323), V321 (= V324), E323 (= E326), G350 (= G352), D352 (= D354), S353 (= S355)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
60% identity, 100% coverage: 2:396/396 of query aligns to 1:394/394 of P18912

query
sites
P18912

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K201 (= K204) binding ATP
N316 (= N319) binding ATP
E323 (= E326) binding ATP
GGDS 350:353 (= GGDS 352:355) binding ATP

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
58% identity, 100% coverage: 2:396/396 of query aligns to 1:397/654 of P36204

query
sites
P36204

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R36 (= R38) binding substrate
R118 (= R120) binding substrate
R151 (= R153) binding substrate

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
59% identity, 100% coverage: 2:396/396 of query aligns to 1:394/394 of P40924

query
sites
P40924

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G

A

A

K

K

V

V

I

L

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

E

P

V

S

V

A

E

E

E

F

L

S

R

L

L

R

T

A

P

V

V

A

A

T

A

R

R

L

L

S

G

D

E

R

L

L

L

G

G

Q

K

E

E

V

V

T

K

M

K

A

A

P

D

D

E

C

A

I

Y

G

G

E

D

K

A

V

V

Q

K

L

A

L

Q

A

I

N

S

Q

E

L

M

Q

K

E

D

G

G

Q

D

V

V

I

L

L

V

L

L

E

E

N

N

V

V

R

R

F

F

H

Y

S

P

G

G

E

E

T

E

D

K

N

N

D

D

P

P

A

E

F

L

A

A

A

K

Q

A

L

F

A

A

A

E

L

L

A

A

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

T

E

A

G

G

V

I

A

A

R

E

L

H

L

L

Q

-

P

P

A

A

V

V

A

A

G

G

F

F

L

L

M

M

E

E

K

K

E

E

L

L

Q

D

Y

V

L

L

G

G

G

K

A

A

L

V

A
x
S

N

N

P

P

Q

D

R

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G

G

A

A

K

K

V

V

S

K

D

D

K

K

I

I

T

G

V

V

I

I

E

E

N

S

L

L

L

L

S

D

K

K

V

V

D

D

T

N

I

L

L

I

I

I

G

G

G

G

G

G

M

L

A

A

Y

Y

T

T

F

F

L

V

K

K

A

A

Q

L

G

G

V

Y

E

E

V

V

G

G

Q

K

S

S

L

L

V

L

E

E

E

E

D

D

R

K

I

I

A

E

L

L

A

A

G

K

D

S

L

F

L

M

D

D

K

R

A

A

Q

K

K

E

Q

K

G

G

I

V

K

N

F

F

L

Y

L

M

P

P

I

E

D

D

H

V

L

L

V

V

A

A

E

D

T

D

F

F

A

S

A

N

D

D

S

A

A

N

F

V

I

K

T

I

V

V

Q

P

N

I

A

S

D

E

F

I

P

P

T

S

T

D

G

L

M

E

G

A

L

I

D

D

I

I

G

G

P

T

A

K

T
|
T

I

R

Q

E

L

T

F

Y

E

A

Q

D

Q

V

I

I

T

K

A

N

A

S

K

K

T

L

V

V

V

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

F

I

A

D

N

L

F

F

A

A

K

Q

G

G

T

T

F

K

A

A

I

V

A

A

K

E

A

A

L

L

A

A

D

E

S

A

-

K

D

D

C

T

M

Y

S

S

I

V

I

I

G

G

G

G

G

G

D

D

S

S

V

A

A

A

A

A

V

V

N

E

K

K

S

F

H

G

L

L

Q

A

Q

D

Q

K

M

M

T

S

H

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

V

A

A

A

A

L

L

T

N

D

D

K

K

S183 (≠ A186) modified: Phosphoserine
T299 (= T302) modified: Phosphothreonine

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
57% identity, 99% coverage: 3:396/396 of query aligns to 1:396/398 of 1vpeA

query
sites
1vpeA

N

E

K

K

K

M

T

T

L

I

K

R

D

D

I

V

N

D

V

L

R

K

G

G

K

K

R

R

V

V

F

I

C

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

V

D

-

S

K

N

D

G

G

K

V

I

V

T

Q

D

D

D

D

T

T

R
|
R

I

I

Q

R

A

A

A

A

L

L

P

P

T

T

I

I

R

K

Y

Y

L

A

I

L

E

E

Q

Q

G

G

A

A

K

K

V

V

I

I

L

L

A

L

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

E

P

P

S

S

A

P

E

E

F

F

S

S

L

L

R

A

A

P

V

V

A

A

T

K

R

R

L

L

S

S

D

E

R

L

L

L

G

G

Q

K

E

E

V

V

T

K

M

F

A

V

P

P

D

A

C

V

I

V

G

G

E

D

K

E

V

V

Q

K

L

K

L

A

A

V

N

E

Q

E

L

L

Q

K

E

E

G

G

Q

E

V

V

I

L

L

L

L

L

E

E

N

N

V

T

R

R

F

F

H

H

S

P

G

G

E

E

T

T

D

K

N

N

D

D

P

P

A

E

F

L

A

A

A

K

Q

F

L

W

A

A

A

S

L

L

A

A

E

D

I

I

Y

H

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

N

E

V

G

G

V

I

A

A

R

Q

L

F

L

I

Q

-

P

P

A

S

V

V

A

A

G

G

F

F

L

L

M

M

E

E

K

K

E

E

L

I

Q

K

Y

F

L

L

G

S

G

K

A

V

L

T

A

Y

N

N

P

P

Q

E

R

K

P

P

F

Y

V

V

A

V

I

V

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

T

G

V

V

I

I

E

T

N

N

L

L

L

M

S

E

K

K

V

A

D

D

T

R

I

I

L

L

I

I

G

G

G
|
G

G
x
A

M

M

A

M

Y

F

T

T

F

F

L

L

K

K

A

A

Q

L

G

G

V

K

E

E

V

V

G

G

Q

S

S

S

L

R

V

V

E

E

E

E

D

D

R

K

I

I

A

D

L

L

A

A

G

K

D

E

L

L

L

V

D

E

K

K

A

A

Q

K

K

E

Q

K

G

G

I

V

K

E

F

I

L

V

L

L

P

P

I

V

D

D

H

A

L

V

V

I

A

A

E

Q

T

K

F

I

A

E

A

P

D

G

S

V

A

E

F

K

I

K

T

V

V

V

Q

R

N

I

A

D

D

D

-

G

F

I

P

P

T

E

T

G

G

W

M

M

G

G

L

L

D

D

I

I

G

G

P

P

A

E

T

T

I

I

Q

E

L

L

F

F

E

K

Q

Q

Q

K

I

L

T

S

A

D

A

A

K

K

T

T

V

V

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

I

A

D

N

D

F

F

A

A

K

E

G

G

T

T

F

K

A

Q

I

V

A

A

K

L

A

A

L

I

A

A

-

A

-

L

-

T

D

E

S

K

D

G

C

A

M

I

S

T

I

V

I

V

G

G

G
|
G

G
|
G

D
|
D

S
|
S

V

A

A

A

A

A

V

V

N

N

K

K

S

F

H

G

L

L

Q

E

Q

D

Q

K

M

F

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

F

L

L

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

I

A

A

A

S

L

M

T

R

D

I

K

K

active site: R35 (= R38), K196 (= K200), G353 (= G353), G376 (= G376)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G198), A195 (= A199), K196 (= K200), K200 (= K204), G218 (= G222), A219 (≠ G223), N316 (= N319), P318 (= P321), G320 (= G323), V321 (= V324), E323 (= E326), G352 (= G352), G353 (= G353), D354 (= D354), S355 (= S355)

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
48% identity, 99% coverage: 4:396/396 of query aligns to 2:415/415 of 16pkA

query
sites
16pkA

K

K

K

K

T

S

L

I

K

N

D

E

I

C

N

D

V

L

R

K

G

G

K

K

R

K

V

V

F

L

C

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

V

D

-

S

K

N

N

G

G

K

K

I

I

T

T

D

N

D

D

T

Y

R
|
R

I

I

Q

R

A

S

A

A

L

L

P

P

T

T

I

L

R

K

Y

K

L

V

I

L

E

T

Q

E

G

G

A

G

K

S

V

C

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

I

P

P

S

M

A

A

E

Q

F

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

-

Q

-

Q

-

K

-

A

S

T

L

L

R

K

A

P

V

V

A

A

T

K

R

R

L

L

S

S

D

E

R

L

L

L

G

L

Q

R

E

P

V

V

T

T

M

F

A

A

P

P

D

D

C

C

I

L

G

-

E

-

K

N

V

A

Q

A

L

D

L

V

A

V

N

S

Q

K

L

M

Q

S

E

P

G

G

Q

D

V

V

I

V

L

L

L

L

E

E

N

N

V

V

R

R

F

F

H

Y

-

K

-

E

-

E

-

G

S

S

G

K

E

K

T

A

D

K

N

D

D

R

P

E

A

A

F

M

A

A

A

K

Q

I

L

L

A

A

A

S

L

Y

A

G

E

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

A

A

S

T

T

M

E

T

G

G

V

I

A

P

R

K

L

I

L

L

Q

G

P

N

A

G

V

A

A

A

G

G

F

Y

L

L

M

M

E

E

K

K

E

E

L

I

Q

S

Y

Y

L

F

G

A

G

K

A

V

L

L

A

G

N

N

P

P

Q

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

T

Q

V

L

I

L

E

D

N

N

L

M

L

L

S

Q

K

R

V

I

D

D

T

Y

I

L

L

L

I

I

G

G

G

G

G
x
A

M

M

A

A

Y
|
Y

T

T

F

F

L

L

K

K

A

A

Q

Q

G

G

V

Y

E

S

V

I

G

G

Q

K

S

S

L

K

V

C

E

E

E

E

D

S

R

K

I

L

A

E

L

F

A

A

G

R

D

S

L

L

L

L

D

K

K

K

A

A

Q

E

K

D

Q

R

G

K

I

V

K

Q

F

V

L

I

L

L

P

P

I

I

D

D

H

H

L

V

V

C

A

H

E

T

T

E

F

F

-

K

A

A

A

V

D

D

S

S

A

P

F

L

I

I

T

T

V

-

Q

E

N

D

A

Q

D

N

F

I

P

P

T

E

T

G

G

H

M

M

G

A

L
|
L

D

D

I

I

G

G

P

P

A

K

T

T

I

I

Q

E

L

K

F

Y

E

V

Q

Q

Q

T

I

I

T

G

A

K

A

C

K

K

T

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

A

V

N

P

F

Y

A

S

K

K

G

G

T

T

F

F

A

A

I

I

A

A

K

K

A

A

L

M

A

G

-

R

-

G

-

T

-

H

D

E

S

H

D

G

C

L

M

M

S

S

I

I

I

I

G

G

G

G

G
|
G

D

D

S

S

V

A

A

S

A

A

V

A

N

E

K

L

S

S

H

G

L

E

Q

A

Q

K

Q

R

M

M

T

S

H

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

G

G

I

V

A

T

A

V

L

L

T

D

D

D

K

K

active site: R35 (= R38), K215 (= K200), G372 (= G353), G395 (= G376)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G198), A214 (= A199), K219 (= K204), A238 (≠ G223), Y241 (= Y226), L311 (= L296), P336 (= P321), G338 (= G323), V339 (= V324), E341 (= E326), G393 (= G374), G394 (= G375), G395 (= G376)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
48% identity, 99% coverage: 4:396/396 of query aligns to 2:415/415 of 13pkA

query
sites
13pkA

K

K

K

K

T

S

L

I

K

N

D

E

I

C

N

D

V

L

R

K

G

G

K

K

R

K

V

V

F

L

C

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

V

D

-

S

K

N

N

G

G

K

K

I

I

T

T

D

N

D

D

T

Y

R
|
R

I

I

Q

R

A

S

A

A

L

L

P

P

T

T

I

L

R

K

Y

K

L

V

I

L

E

T

Q

E

G

G

A

G

K

S

V

C

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

I

P

P

S

M

A

A

E

Q

F

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

-

Q

-

Q

-

K

-

A

S

T

L

L

R

K

A

P

V

V

A

A

T

K

R

R

L

L

S

S

D

E

R

L

L

L

G

L

Q

R

E

P

V

V

T

T

M

F

A

A

P

P

D

D

C

C

I

L

G

-

E

-

K

N

V

A

Q

A

L

D

L

V

A

V

N

S

Q

K

L

M

Q

S

E

P

G

G

Q

D

V

V

I

V

L

L

L

L

E

E

N

N

V

V

R

R

F

F

H

Y

-

K

-

E

-

E

-

G

S

S

G

K

E

K

T

A

D

K

N

D

D

R

P

E

A

A

F

M

A

A

A

K

Q

I

L

L

A

A

A

S

L

Y

A

G

E

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

A

A

S

T

T

M

E

T

G

G

V

I

A

P

R

K

L

I

L

L

Q

G

P

N

A

G

V

A

A

A

G

G

F

Y

L

L

M

M

E

E

K

K

E

E

L

I

Q

S

Y

Y

L

F

G

A

G

K

A

V

L

L

A

G

N

N

P

P

Q

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

T

Q

V

L

I

L

E

D

N

N

L

M

L

L

S

Q

K

R

V

I

D

D

T

Y

I

L

L

L

I

I

G

G

G

G

G

A

M

M

A

A

Y

Y

T

T

F

F

L

L

K

K

A

A

Q

Q

G

G

V

Y

E

S

V

I

G

G

Q

K

S

S

L

K

V

C

E

E

E

E

D

S

R

K

I

L

A

E

L

F

A

A

G

R

D

S

L

L

L

L

D

K

K

K

A

A

Q

E

K

D

Q

R

G

K

I

V

K

Q

F

V

L

I

L

L

P

P

I

I

D

D

H

H

L

V

V

C

A

H

E

T

T

E

F

F

-

K

A

A

A

V

D

D

S

S

A

P

F

L

I

I

T

T

V

-

Q

E

N

D

A

Q

D

N

F

I

P

P

T

E

T

G

G

H

M

M

G

A

L
|
L

D

D

I

I

G

G

P

P

A

K

T

T

I

I

Q

E

L

K

F

Y

E

V

Q

Q

Q

T

I

I

T

G

A

K

A

C

K

K

T

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

A

V

N

P

F

Y

A

S

K

K

G

G

T

T

F

F

A

A

I

I

A

A

K

K

A

A

L

M

A

G

-

R

-

G

-

T

-

H

D

E

S

H

D

G

C

L

M

M

S

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

V

A

A

S

A

A

V

A

N

E

K

L

S

S

H

G

L

E

Q

A

Q

K

Q

R

M

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

G

G

I

V

A

T

A

V

L

L

T

D

D

D

K

K

active site: R35 (= R38), K215 (= K200), G372 (= G353), G395 (= G376)
binding adenosine-5'-diphosphate: G213 (= G198), A214 (= A199), K219 (= K204), L311 (= L296), P336 (= P321), G338 (= G323), V339 (= V324), E341 (= E326), G371 (= G352), D373 (= D354), S374 (= S355)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
47% identity, 99% coverage: 4:396/396 of query aligns to 6:419/440 of P07378

query
sites
P07378

K

K

K

K

T

S

L

I

K

N

D

E

I

C

N

D

V

L

R

K

G

G

K

K

R

K

V

V

F

L

C

I

R

R

C

V

D
|
D

F
|
F

N
|
N

V

V

P

P

I

V

D

-

S

K

N

N

G

G

K

K

I

I

T

T

D

N

D

D

T

Y

R
|
R

I

I

Q

R

A

S

A

A

L

L

P

P

T

T

I

L

R

K

Y

K

L

V

I

L

E

T

Q

E

G

G

A

G

K

S

V

C

I

V

L

L

A

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

K

K

G

G

K

I

P

P

S

M

A

A

E

Q

F

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

-

Q

-

Q

-

K

-

A

S

T

L

L

R

K

A

P

V

V

A

A

T

K

R

A

L

L

S

S

D

E

R

L

L

L

G

L

Q

R

E

P

V

V

T

T

M

F

A

A

P

P

D

D

C

C

I

L

G

-

E

-

K

N

V

A

Q

A

L

D

L

V

A

V

N

S

Q

K

L

M

Q

S

E

P

G

G

Q

D

V

V

I

V

L

L

L

L

E

E

N

N

V

V

R
|
R

F

F

H

Y

-

K

-

E

-

E

-

G

S

S

G

K

E

K

T

A

D

K

N

D

D

R

P

E

A

A

F

M

A

A

A

K

Q

I

L

L

A

A

A

S

L

Y

A

G

E

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

D

H

S

A

A

S

T

T

M

E

T

G

G

V

I

A

P

R

K

L

I

L

L

Q

G

P

N

A

G

V

A

A

A

G

G

F

Y

L

L

M

M

E

E

K

K

E

E

L

I

Q

S

Y

Y

L

F

G

A

G

K

A

V

L

L

A

G

N

N

P

P

Q

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G

G

A

A

K

K

V

V

S

S

D

D

K
|
K

I

I

T

Q

V

L

I

L

E

D

N

N

L

M

L

L

S

Q

K

R

V

I

D

D

T

Y

I

L

L

L

I

I

G

G

G

G

G

A

M

M

A

A

Y

Y

T

T

F

F

L

L

K

K

A

A

Q

Q

G

G

V

Y

E

S

V

I

G

G

Q

K

S

S

L

K

V

C

E

E

E

E

D

S

R

K

I

L

A

E

L

F

A

A

G

R

D

S

L

L

L

L

D

K

K

K

A

A

Q

E

K

D

Q

R

G

K

I

V

K

Q

F

V

L

I

L

L

P

P

I

I

D

D

H

H

L

V

V

C

A

H

E

T

T

E

F

F

-

K

A

A

A

V

D

D

S

S

A

P

F

L

I

I

T

T

V

-

Q

E

N

D

A

Q

D

N

F

I

P

P

T

E

T

G

G

H

M

M

G

A

L

L

D

D

I

I

G

G

P

P

A

K

T

T

I

I

Q

E

L

K

F

Y

E

V

Q

Q

Q

T

I

I

T

G

A

K

A

C

K

K

T

S

V

A

V

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

F

M

A

V

N

P

F

Y

A

S

K

K

G

G

T

T

F

F

A

A

I

I

A

A

K

K

A

A

L

M

A

G

-

R

-

G

-

T

-

H

D

E

S

H

D

G

C

L

M

M

S

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

V

A

A

S

A

A

V

A

N

E

K

L

S

S

H

G

L

E

Q

A

Q

K

Q

R

M

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

G

G

I

V

A

T

A

V

L

L

T

D

D

E

K

K

DFN 24:26 (= DFN 22:24) binding substrate
R39 (= R38) binding substrate
HLGR 62:65 (= HLGR 61:64) binding substrate
R135 (= R120) binding substrate
R172 (= R153) binding substrate
K223 (= K204) binding ATP
N338 (= N319) binding ATP
E345 (= E326) binding ATP
GGDS 375:378 (= GGDS 352:355) binding ATP

3zlbA Crystal structure of phosphoglycerate kinase from streptococcus pneumoniae (see paper)
51% identity, 100% coverage: 2:396/396 of query aligns to 1:398/398 of 3zlbA

query
sites
3zlbA

L
x
M

N

A

K

K

K

L

T

T

L

V

K

K

D
|
D

I

V

N

D

V

L

R

K

G

G

K

K

R

K

V

V

F

L

C

V

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

L

D

-

S

K

N

D

G

G

K

V

I

I

T

T

D

N

D

D

T

N

R
|
R

I

I

Q

T

A

A

A

A

L

L

P

P

T

T

I

I

R

K

Y

Y

L

I

I

I

E

E

Q

Q

G

G

A

G

K

R

V

A

I

I

L

L

A

F

S

S

H

H

L

L

G

G

R

R

P

V

K

K

G

E

K

E

P

S

-

D

S

K

A

A

E

G

F

K

S

S

L

L

R

A

A

P

V

V

A

A

T

A

R

D

L

L

S

A

D

A

R

K

L

L

G

G

Q

Q

E

D

V

V

T

V

M

F

A

P

P

G

D

V

C

T

I

R

G

G

E

A

K

E

V

L

Q

E

L

A

L

A

A

I

N

N

Q

A

L

L

Q

E

E

D

G

G

Q

Q

V

V

I

L

L

L

L

V

E

E

N

N

V

T

R

R

F

Y

H

E

-

D

-

V

-

D

-

G

S

K

G

K

E

E

T

S

D

K

N

N

D

D

P

P

A

E

F

L

A

G

A

K

Q

Y

L

W

A

A

A

S

L

L

A

G

E

D

-

G

I

I

Y

F

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

N

E

V

G

G

V

I

A

S

R

A

L

N

L

V

Q

E

P

K

A

A

V

V

A

A

G

G

F

F

L

L

M

L

E

E

K

N

E

E

L

I

Q

A

Y

Y

L

I

G

Q

G

E

A

A

L

V

A

E

N

T

P

P

Q

E

R

R

P

P

F

F

V

V

A

A

I

I

L

L

G

G

G
|
G

A
x
S

K
|
K

V

V

S

S

D

D

K

K

I

I

T

G

V

V

I

I

E

E

N

N

L

L

L

L

S

E

K

K

V

A

D

D

T

K

I

V

L

L

I

I

G

G

G
|
G

G
|
G

M

M

A

T

Y

Y

T

T

F

F

L

Y

K

K

A

A

Q

Q

G

G

V

I

E

E

V

I

G

G

Q

N

S

S

L

L

V

V

E

E

E

E

D

D

R

K

I

L

A

D

L

V

A

A

G

K

D

A

L

L

L

L

D

E

K

K

A

A

Q

N

K

G

Q

-

G

-

I

-

K

K

F

L

L

I

L

L

P

P

I

V

D

D

H

S

L

K

V

E

A

A

E

N

T

A

F

F

A

A

A

G

D

Y

S

T

A

E

F

V

I

R

T

D

V

T

Q

E

N

G

A

E

D

A

F

V

P

S

T

E

T

G

G

F

M

L

G

G

L

L

D

D

I

I

G

G

P

P

A

K

T

S

I

I

Q

A

L

K

F

F

E

D

Q

E

Q

A

I

L

T

T

A

G

A

A

K

K

T

T

V

V

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

N

A

P

N

D

F

F

A

Q

K

A

G

G

T

T

F

I

A

G

I

V

A

M

K

D

A

A

L

I

A

V

D

K

S

Q

D

P

C

G

M

V

-

K

S

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

V

A

A

A

A

A

V

A

N

I

K

N

S

L

H

G

L

R

Q

A

Q

D

Q

K

M

F

T

S

H

W

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

M

E

E

F

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

L

A

A

A

A

L

L

T

T

D

E

K
|
K

active site: R36 (= R38), K204 (= K200), G355 (= G353), G378 (= G376)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G198), S203 (≠ A199), G226 (= G222), G227 (= G223), N320 (= N319), P322 (= P321), G324 (= G323), V325 (= V324), E327 (= E326), G354 (= G352), G355 (= G353), D356 (= D354), S357 (= S355)
binding magnesium ion: M1 (≠ L2), D8 (= D9), K398 (= K396)

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
48% identity, 99% coverage: 3:395/396 of query aligns to 3:404/405 of 2wzcA

query
sites
2wzcA

N

N

K

K

K

L

T

T

L

L

K

D

D

K

I

L

N

D

V

V

R

K

G

G

K

K

R

R

V

V

F

V

C

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

M

D

K

S

N

N

N

G

-

K

Q

I

I

T

T

D

N

D

N

T

Q

R
|
R

I

I

Q

K

A

A

A

A

L

V

P

P

T

S

I

I

R

K

Y

F

L

C

I

L

E

D

Q

N

G

G

A

A

K

K

-

S

V

V

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

V

P

P

S

M

A

P

E

D

-

K

F

Y

S

S

L

L

R

E

A

P

V

V

A

A

T

V

R

E

L

L

S

K

D

S

R

L

L

L

G

G

Q

K

E

D

V

V

T

L

M

F

A

L

P

K

D

D

C

C

I

V

G

G

E

P

K

E

V

V

Q

E

L

K

L

A

A

C

N

A

Q

N

L

P

Q

A

E

A

G

G

Q

S

V

V

I

I

L

L

L

L

E

E

N

N

V

L

R

R

F

F

H

H

-

V

-

E

-

E

-

E

-

G

S

K

G

G

E

E

T

P

D

A

N

K

D

I

P

E

A

A

F

F

A

R

A

A

Q

S

L

L

A

S

A

K

L

L

A

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

V

V

A

-

R

N

L

L

L

P

Q

Q

P

K

A

A

V

-

A

G

G

G

F

F

L

L

M

M

E

K

K

K

E

E

L

L

Q

N

Y

Y

L

F

G

A

G

K

A

A

L

L

A

E

N

S

P

P

Q

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K
|
K

I

I

T

Q

V

L

I

I

E

N

N

N

L

M

L

L

S

D

K

K

V

V

D

N

T

E

I

M

L

I

I

I

G

G

G
|
G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

V

M

E

E

V

I

G

G

Q

T

S

S

L

L

V

F

E

D

E

E

D

E

R

G

I

A

A

K

L

I

A

V

G

K

D

D

L

L

L

M

D

S

K

K

A

A

Q

E

K

K

Q

N

G

G

I

V

K

K

F

I

L

T

L

L

P

P

I

V

D

D

H

F

L

V

V

T

A

A

E

D

T

K

F

F

-

D

-

E

A

N

A

A

D

K

S

T

A

G

F

Q

I

A

T

T

V

V

Q

A

N

S

A

G

D

-

F

I

P

P

T

A

T

G

G

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

I

S

Q

K

L

K

F

Y

E

A

Q

E

Q

A

I

V

T

T

A

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

A

E

N

A

F

F

A

A

K

R

G

G

T

T

F

K

A

A

I

L

A

M

K

D

A

E

L

V

A

V

D

K

S

A

D

T

-

S

-

R

-

G

C

C

M

I

S

T

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

V

A

A

T

A

C

V

C

N

A

K

K

S

W

H

N

L

T

Q

E

Q

D

Q

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

T

S

D

N

active site: R37 (= R38), K204 (= K200), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G198), A203 (= A199), K204 (= K200), K208 (= K204), G226 (= G222), G227 (= G223), N325 (= N319), P327 (= P321), G329 (= G323), V330 (= V324), E332 (= E326), G361 (= G352), D363 (= D354), T364 (≠ S355)
binding tetrafluoroaluminate ion: R37 (= R38), K204 (= K200), K208 (= K204), G361 (= G352), G362 (= G353), G384 (= G375)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
48% identity, 99% coverage: 3:395/396 of query aligns to 3:404/405 of 2wzbA

query
sites
2wzbA

N

N

K

K

K

L

T

T

L

L

K

D

D

K

I

L

N

D

V

V

R

K

G

G

K

K

R

R

V

V

F

V

C

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

M

D

K

S

N

N

N

G

-

K

Q

I

I

T

T

D

N

D

N

T

Q

R
|
R

I

I

Q

K

A

A

A

A

L

V

P

P

T

S

I

I

R

K

Y

F

L

C

I

L

E

D

Q

N

G

G

A

A

K

K

-

S

V

V

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

V

P

P

S

M

A

P

E

D

-

K

F

Y

S

S

L

L

R

E

A

P

V

V

A

A

T

V

R

E

L

L

S

K

D

S

R

L

L

L

G

G

Q

K

E

D

V

V

T

L

M

F

A

L

P

K

D

D

C

C

I

V

G

G

E

P

K

E

V

V

Q

E

L

K

L

A

A

C

N

A

Q

N

L

P

Q

A

E

A

G

G

Q

S

V

V

I

I

L

L

L

L

E

E

N

N

V

L

R

R

F

F

H

H

-

V

-

E

-

E

-

E

-

G

S

K

G

G

E

E

T

P

D

A

N

K

D

I

P

E

A

A

F

F

A

R

A

A

Q

S

L

L

A

S

A

K

L

L

A

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

V

V

A

-

R

N

L

L

L

P

Q

Q

P

K

A

A

V

-

A

G

G

G

F

F

L

L

M

M

E

K

K

K

E

E

L

L

Q

N

Y

Y

L

F

G

A

G

K

A

A

L

L

A

E

N

S

P

P

Q

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

D

D

K
|
K

I

I

T

Q

V

L

I

I

E

N

N

N

L

M

L

L

S

D

K

K

V

V

D

N

T

E

I

M

L

I

I

I

G

G

G
|
G

G
|
G

M

M

A

A

Y

F

T

T

F

F

L

L

K

K

A

V

-

L

Q

N

G

N

V

M

E

E

V

I

G

G

Q

T

S

S

L

L

V

F

E

D

E

E

D

E

R

G

I

A

A

K

L

I

A

V

G

K

D

D

L

L

L

M

D

S

K

K

A

A

Q

E

K

K

Q

N

G

G

I

V

K

K

F

I

L

T

L

L

P

P

I

V

D

D

H

F

L

V

V

T

A

A

E

D

T

K

F

F

-

D

-

E

A

N

A

A

D

K

S

T

A

G

F

Q

I

A

T

T

V

V

Q

A

N

S

A

G

D

-

F

I

P

P

T

A

T

G

G

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

I

S

Q

K

L

K

F

Y

E

A

Q

E

Q

A

I

V

T

T

A

R

A

A

K

K

T

Q

V

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

F

W

A

E

N

A

F

F

A

A

K

R

G

G

T

T

F

K

A

A

I

L

A

M

K

D

A

E

L

V

A

V

D

K

S

A

D

T

-

S

-

R

-

G

C

C

M

I

S

T

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

V

A

A

T

A

C

V

C

N

A

K

K

S

W

H

N

L

T

Q

E

Q

D

Q

K

M

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

T

S

D

N

active site: R37 (= R38), K204 (= K200), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G198), A203 (= A199), K204 (= K200), K208 (= K204), G226 (= G222), G227 (= G223), N325 (= N319), P327 (= P321), G329 (= G323), V330 (= V324), E332 (= E326), G361 (= G352), D363 (= D354), T364 (≠ S355)
binding trifluoromagnesate: K204 (= K200), K208 (= K204), G361 (= G352), G384 (= G375), G385 (= G376)

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
48% identity, 99% coverage: 3:395/396 of query aligns to 3:406/407 of 4axxA

query
sites
4axxA

N

N

K

K

K

L

T

T

L

L

K

D

D

K

I

L

N

D

V

V

R

K

G

G

K

K

R

R

V

V

F

V

C

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

I

M

D

K

S

N

N

N

G

-

K

Q

I

I

T

T

D

N

D

N

T