SitesBLAST
Comparing WP_092350915.1 NCBI__GCF_900107645.1:WP_092350915.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
37% identity, 99% coverage: 3:528/531 of query aligns to 4:525/526 of 3dc2A
- active site: N96 (= N97), R230 (= R231), D254 (= D255), E259 (= E260), H277 (= H281)
- binding serine: Y458 (= Y461), D460 (= D463), R461 (= R464), P462 (≠ S465), G463 (= G466), A464 (≠ L467), L465 (≠ I468), L484 (= L487)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
37% identity, 99% coverage: 3:528/531 of query aligns to 5:524/525 of 3ddnB
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
38% identity, 85% coverage: 2:453/531 of query aligns to 8:456/533 of O43175
- T78 (≠ V73) binding NAD(+)
- R135 (= R130) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ KV 150:151) binding NAD(+)
- D175 (= D170) binding NAD(+)
- T207 (= T202) binding NAD(+)
- CAR 234:236 (= CAR 229:231) binding NAD(+)
- D260 (= D255) binding NAD(+)
- V261 (= V256) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (= HLGA 281:284) binding NAD(+)
- A373 (≠ S371) to T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- G377 (≠ A375) to S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- V425 (≠ L418) to M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
44% identity, 58% coverage: 2:307/531 of query aligns to 4:305/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
44% identity, 57% coverage: 2:305/531 of query aligns to 4:303/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V73), A102 (= A101), G148 (= G147), R151 (≠ K150), I152 (≠ V151), Y170 (= Y169), D171 (= D170), P172 (= P171), I173 (≠ Y172), H202 (= H201), T203 (= T202), P204 (= P203), T209 (= T208), C230 (= C229), A231 (= A230), R232 (= R231), H279 (= H281), G281 (= G283)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ G13), K17 (≠ P16), I18 (≠ L17), E293 (≠ V295)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
44% identity, 57% coverage: 2:305/531 of query aligns to 3:302/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I146), G147 (= G147), L148 (= L148), G149 (= G149), R150 (≠ K150), I151 (≠ V151), G152 (= G152), D170 (= D170), H201 (= H201), T202 (= T202), P203 (= P203)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
44% identity, 57% coverage: 2:305/531 of query aligns to 3:302/302 of 6rihA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
44% identity, 57% coverage: 2:305/531 of query aligns to 3:302/303 of 6plgA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
44% identity, 57% coverage: 2:306/531 of query aligns to 2:299/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N97), A100 (= A101), R149 (≠ K150), I150 (≠ V151), Y168 (= Y169), D169 (= D170), P170 (= P171), I171 (≠ Y172), H200 (= H201), T201 (= T202), P202 (= P203), T207 (= T208), C228 (= C229), A229 (= A230), R230 (= R231), H277 (= H281), G279 (= G283)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
44% identity, 57% coverage: 2:306/531 of query aligns to 3:300/301 of 6rj5A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
44% identity, 57% coverage: 3:305/531 of query aligns to 1:299/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G149), I148 (≠ V151), Y166 (= Y169), D167 (= D170), P168 (= P171), I169 (≠ Y172), I170 (= I173), H198 (= H201), T199 (= T202), L208 (≠ I211), R228 (= R231)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
44% identity, 56% coverage: 2:298/531 of query aligns to 2:294/297 of 6rj3A
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
43% identity, 57% coverage: 2:304/531 of query aligns to 2:292/292 of 6plfB