SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_092481889.1 NCBI__GCF_900115975.1:WP_092481889.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8xcsA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADPH, akg and nh4 in the initial stage of reaction (see paper)
57% identity, 98% coverage: 4:417/421 of query aligns to 2:416/418 of 8xcsA

query
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binding 2-oxoglutaric acid: K68 (= K70), G70 (= G72), M89 (= M91), K92 (= K94), K104 (= K106), A142 (= A144), D144 (= D146), G346 (= G346), S350 (= S350)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R72 (= R74), K112 (= K114), P143 (= P145), D144 (= D146), V145 (= V147), Y146 (≠ G148), T190 (= T190), Y219 (≠ F219), G220 (= G220), N221 (= N221), A222 (≠ V222), D243 (= D243), S244 (= S244), K263 (= K263), A295 (= A295), I296 (≠ L296), N318 (= N318)

8xd5A Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction (see paper)
57% identity, 98% coverage: 4:417/421 of query aligns to 3:417/419 of 8xd5A

query
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8xd5A

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binding gamma-l-glutamic acid: K69 (= K70), M90 (= M91), K105 (= K106), A143 (= A144), D145 (= D146), S351 (= S350)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R73 (= R74), D145 (= D146), V146 (= V147), Y147 (≠ G148), T191 (= T190), Y220 (≠ F219), G221 (= G220), N222 (= N221), A223 (≠ V222), D244 (= D243), S245 (= S244), K264 (= K263), N281 (≠ T280), A295 (= A294), A296 (= A295), I297 (≠ L296), N319 (= N318), N344 (= N343)

8xcoA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus incorporating NADPH in the initial stage of reaction (see paper)
57% identity, 98% coverage: 5:417/421 of query aligns to 1:414/416 of 8xcoA

query
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8xcoA

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binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T188 (= T190), Y217 (≠ F219), G218 (= G220), N219 (= N221), A220 (≠ V222), D241 (= D243), S242 (= S244), K261 (= K263), I294 (≠ L296)

8zneA Cryo-em structure of w89f mutated glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction
57% identity, 98% coverage: 5:417/421 of query aligns to 1:414/416 of 8zneA

query
sites
8zneA

I

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I

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S

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Y

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binding glutamic acid: M87 (= M91), K90 (= K94), A140 (= A144), D142 (= D146), S348 (= S350)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G216 (= G218), Y217 (≠ F219), G218 (= G220), N219 (= N221), A220 (≠ V222), D241 (= D243), S242 (= S244), A293 (= A295), I294 (≠ L296)

P39633 Catabolic NAD-specific glutamate dehydrogenase RocG; NAD-GDH; Glutamate dehydrogenase; GlutDH; Trigger enzyme RocG; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see 2 papers)
52% identity, 99% coverage: 3:417/421 of query aligns to 13:424/424 of P39633

query
sites
P39633

Q

E

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A

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W

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A

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Y

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x
A

A

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E27 (≠ D17) mutation to F: Increase of thermostability 8 degrees Celsius higher than that of the wild-type.
E93 (= E83) mutation to K: Reduces the affinity for glutamate and ammonium.
D122 (≠ N112) mutation to N: Unable to control gltAB expression via an inhibitory interactions with the transcriptional regulator GltC. Reduces the affinity for glutamate and ammonium.
Q144 (≠ P134) mutation to R: Increase of thermostability 20 degrees Celsius higher than that of the wild-type.
Y158 (≠ G148) mutation to H: Reduces the affinity for glutamate and ammonium.
S234 (= S224) mutation to R: Reduces the affinity for glutamate and ammonium.
A324 (≠ G316) mutation to R: No effect.

P50735 Cryptic catabolic NAD-specific glutamate dehydrogenase GudB; NAD-GDH; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see paper)
54% identity, 98% coverage: 4:417/421 of query aligns to 14:427/427 of P50735

query
sites
P50735

E

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A

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A

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D

L

I

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K

D

G

A

A

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Q

G

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F

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N

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A

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A

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N

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E

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N

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A

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A

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K

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I

VKA 97:99 (vs. gap) mutation Missing: In gudB1; gains glutamate dehydrogenase activity, restores growth on proline, arginine, ornithine.

P28997 NAD-specific glutamate dehydrogenase; NAD-GDH; EC 1.4.1.2 from Peptoniphilus asaccharolyticus (Peptostreptococcus asaccharolyticus) (see 2 papers)
52% identity, 99% coverage: 1:416/421 of query aligns to 1:420/421 of P28997

query
sites
P28997

M

M

S

T

Q

D

E

T

I

L

N

N

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F

L

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R

P

T

A

V

V

A

Y

E

E

I

L

L

L

R

K

E

E

P

P

Q

Q

R

R

V

V

L

I

S

E

V

I

S

S

I

I

P

P

V

V

Q

K

M

M

D

D

G

G

S

T

I

V

K

K

V

V

F

F

K

K

G

G

F

W

R

R

S

S

Q

A

H

H

N

S

A

A

C

V

G

G

P

P

C

S

K

K

G

G

I

V

R

R

F

F

H

H

P

P

D

N

V

V

S

N

M

M

D

D

E

E

V

V

K

K

A

A

L

L

S

S

T

L

W

W

M

M

S

T

L

F

K

K

C

G

A

G

V

A

V

L

N

G

L

L

P

P

Y

Y

G

G

K

K

G

G

V

I

I

C

V

V

N

D

H

P

K

A

E

E

L

L

S

S

P

E

N

R

E

E

L

L

E

E

Q

Q

L

L

C

S

R

R

G

G

Y

W

I

V

R

R

A

G

I

L

A

Y

P

K

V

Y

L

L

G

G

A

D

D

R

M

I

D

D

V

I

P

P

A

A

P

P

D

D

V

V

G

N

T

T

N

N

G

G

Q

Q

T

I

M

M

G

S

W

W

M

F

M

V

D

D

E

E

F

Y

A

V

R

K

Y

L

R

N

G

G

Y

E

N

R

-

M

E

D

F

I

G

G

V

T

I

F

T

T

G

G

K

K

P

P

I

V

I

A

V

F

G

G

S

S

L

E

G

G

R

R

V

N

E

E

A

A

T

T

S

G

R

F

G

G

C

V

V

A

F

V

T

V

I

V

R

R

Q

E

A

S

A

A

K

K

A

R

T

F

G

G

L

I

E

K

L

M

K

E

D

D

A

A

T

K

V

I

V

A

V

V

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

T

I

F

A

T

A

V

R

K

L

N

L

I

S

E

R

R

E

Q

D

G

G

G

C

-

K

K

V

V

L

C

A

A

V

I

S

A

-
x
E

-
x
W

-
x
D

-

R

-

N

D

E

S

G

T

N

G

Y

A

A

A

L

Y

Y

D

N

P

E

N

N

G

G

L

I

D

D

I

F

A

K

A

E

L

L

E

L

K

A

F

Y

K

K

L

E

E

A

T

N

G

K

S

T

V

L

V

I

G

G

F

F

P

P

G

G

S

A

K

E

N

R

I

I

T

T

T

D

E

E

L

F

L

W

Q

T

L

K

E

E

C

Y

D

D

I

I

L

I

V

V

P

P

A

A

L

L

E

E

N

N

Q

V

I

I

T

T

G

G

E

E

N

R

A

A

D

K

K

T

I

I

N

N

A

A

K

K

I

L

V

V

A

C

E

E

G

A

A

A

N

N

G

G

P

P

T

T

P

P

E

E

A

G

D

D

N

K

I

V

L

L

A

T

K

E

R

R

N

G

I

I

L

N

V

L

L

T

P

P

D

D

V

I

L

L

A

T

N

N

A

S

G

G

V

V

T

L

V

V

S

S

Y

Y

F

Y

E

E

W

W

V

V

Q

Q

N

N

Q

Q

Y

Y

G

G

Y

Y

W

W

T

T

E

E

E

A

E

E

V

V

N

E

E

E

R

K

L

Q

E

E

R

A

K

D

M

M

V

M

E

K

A

A

F

I

D

K

S

G

V

V

Y

F

S

A

L

V

Y

A

K

D

E

E

H

Y

G

-

D

N

I

V

N

T

M

L

R

R

G

E

A

A

A

V

Y

Y

M

M

V

Y

A

A

I

I

K

K

R

S

I

I

Y

D

E

V

A

A

M

M

K

K

I

L

R

R

G

G

W

W

E243 (vs. gap) Important for nucleotide recognition; mutation to D: Shows a 9-fold relaxation of the strong discrimination against NADPH due to the decrease of binding affinity for NADH and the increase for NADPH.; mutation to K: Severely crippled in its ability to bind to NADH. Decrease of binding affinity for NADH and increase for NADPH.; mutation to R: Decrease of binding affinity for NADH and increase for NADPH.
W244 (vs. gap) mutation to S: Decrease of binding affinity for NADH and increase for NADPH.
D245 (vs. gap) mutation to K: Decrease of binding affinity for NADH and increase for NADPH.

4xgiA Crystal structure of glutamate dehydrogenase from burkholderia thailandensis
51% identity, 95% coverage: 15:415/421 of query aligns to 21:413/416 of 4xgiA

query
sites
4xgiA

I

V

D

D

D

R

C

V

V

A

E

P

K

Y

L

L

G

G

L

S

D

L

R

S

T

R

V

W

A

I

E

E

I

T

L

L

R

K

E

R

P

P

Q

K

R

R

V

I

L

L

S

I

V

V

S

D

I

V

P

P

V

I

Q

E

M

L

D

D

D

N

G

G

S

T

I

V

K

A

V

H

F

F

K

E

G

G

F

Y

R

R

S

V

Q

Q

H

H

N

N

N

V

A

S

C

R

G

G

P

P

C

G

K
|
K

G

G

G
|
G

I

V

R
|
R

F

Y

H

H

P

Q

D

D

V

V

S

T

M

L

D

S

E

E

V

V

K

M

A

A

L

L

S

S

T

A

W

W

M
|
M

S

S

L

V

K
|
K

C

N

A

A

V

A

V

V

N

N

L

V

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

I

R

V

V

N

D

H

P

K

R

E

K

L

L

S

S

P

R

N

G

E

E

L

L

E

E

Q

R

L

V

C

T

R

R

G

R

Y

Y

I

T

R

S

A

E

I

I

A

G

P

I

V

I

L

I

G

G

A

P

D

N

M

T

D

D

V

I

P

P

A
|
A

P

P

D
|
D

V
|
V

G

N

T

T

N

N

G

E

Q

Q

T

I

M

M

G

A

W

W

M

M

D

D

E

T

F

Y

A

S

R

M

Y

N

R

Q

G

G

Y

Q

N

T

E

A

F

T

G

G

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

S

V

L

G

G

S

S

L

L

G

G

R
|
R

V

K

E

E

A

A

T
|
T

S

G

R

R

G

G

C

V

V

F

F

V

T

V

I

G

R

C

Q

E

A

A

K

K

A

K

T

K

G

G

L

V

E

E

L

I

K

E

D

G

A

A

T

R

V

I

V

A

V

V

Q

Q

G
|
G

F

F

G
|
G

N
|
N

V
|
V

G

G

S

G

I

I

A

A

R

K

L

L

L

F

S

-

R

Q

E

E

D

A

G

G

C

A

K

K

V

V

L

I

A

A

V

V

S

Q

D
|
D

S
x
H

T

T

G

G

A

T

A

I

Y

H

D

Q

P

P

N

A

G

G

L

V

D

D

I

T

A

A

A

K

L

L

E

L

K

D

F

H

K

V

L

G

E

R

T

T

G

G

S

G

V

V

V

A

G

G

F

F

P

E

G

G

S

A

K

E

N

P

I

M

T

P

T

N

E

D

E

E

L

F

L

W

Q

T

L

V

E

E

C

T

D

E

I

I

L

L

V

I

P

P

A
|
A

L

L

E

E

N

N

Q

Q

I

I

T

T

G

E

E

K

N

N

A

A

D

S

K

K

I

I

N

R

A

T

K

K

I

I

V

I

A

V

E

E

G

G

A
|
A

N
|
N

G

G

P

P

T

T

P

T

E

A

A

A

D

D

N

D

I

I

L

L

A

S

K

A

R

N

N

G

I

V

L

L

V

V

L

I

P

P

D

D

V

V

L

I

A

A

N
|
N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

-

Q

-

Y

-

G

-

Y

-

W

W

T

T

E

E

E

D

E

E

V

I

N

N

E

H

R

R

L

L

E

E

R

R

K

V

M

M

V

R

E

E

A

A

F

F

D

A

S

G

V

V

Y

W

S

A

L

V

Y

A

K

E

E

E

H

H

G

-

D

K

I

V

N

S

M

V

R

R

G

T

A

A

Y

F

M

I

V

V

A

A

I

C

K

K

R

R

I

I

Y

L

E

M

A

A

M

R

K

E

I

M

R

R

G

G

active site: K112 (= K106), D152 (= D146)
binding 2-oxoglutaric acid: K76 (= K70), G78 (= G72), M97 (= M91), K100 (= K94), K112 (= K106), A150 (= A144), R192 (= R186), S355 (= S350)
binding nicotinamide-adenine-dinucleotide: R80 (= R74), D152 (= D146), V153 (= V147), T196 (= T190), G224 (= G218), G226 (= G220), N227 (= N221), V228 (= V222), D248 (= D243), H249 (≠ S244), A299 (= A294), A300 (= A295), A322 (= A317), N323 (= N318), N348 (= N343)

3aogA Crystal structure of glutamate dehydrogenase (gdhb) from thermus thermophilus (glu bound form)
51% identity, 97% coverage: 8:415/421 of query aligns to 13:418/421 of 3aogA

query
sites
3aogA

F

W

E

E

I

I

V

F

Q

T

N

E

L

Q

I

V

D

D

D

R

C

V

V

V

E

P

K

Y

L

L

G

G

L

R

D

L

R

A

T

P

V

L

A

A

E

E

I

S

L

L

R

K

E

R

P

P

Q

K

R

R

V

V

L

L

S

I

V

V

S

D

I

V

P

P

V

V

Q

R

M

L

D

D

G

G

S

S

I

V

K

A

V

Y

F

F

K

E

G

G

F

Y

R

R

S

V

Q

H

H

H

N

N

N

T

A
|
A

C

R

G

G

P

P

C

A

K

K

G

G

G
|
G

I

V

R

R

F

Y

H

H

P

P

D

E

V

V

S

T

M

L

D

S

E

E

V

V

K

M

A

A

L

L

S

A

T

G

W

W

M
|
M

S

T

L

I

K

K

C

N

A

A

V

A

V

V

N

G

L

L

P

P

Y

Y

G

G

K
|
K

G

G

V

I

I

R

V

V

N

D

H

P

K

R

E

K

L

L

S

S

P

P

N

G

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

R

Y

Y

I

T

R

S

A

E

I

I

A

G

P

I

V

L

L

L

G

G

A

P

D

D

M

R

D

D

V

I

P

P

A

A

P
|
P

D
|
D

V

V

G

N

T

T

N

G

G

E

Q

R

T

E

M

M

G

A

W

W

M

M

D
|
D

E

T

F

Y

A

S

R
x
M

Y

N

R

V

G

G

Y

R

N

T

E

V

F

P

G

G

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

A

V

L

G

G

S

S

L

L

G

G

R

R

V

R

E

D

A

A

T

T

S

G

R

R

G

G

C

V

V

F

F

I

T

T

I

A

R

A

Q

A

A

A

K

E

A

K

T

I

G

G

L

L

E

Q

L

V

K

E

D

G

A

A

T

R

V

V

V

A

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

N

I

A

A

A

R

R

L

A

L

F

S

-

R

H

E

D

D

H

G

G

C

A

K

R

V

V

L

V

A

A

V

V

S

Q

D

D

S

H

T

T

G

G

A

T

A

V

Y

Y

D

N

P

E

N

A

G

G

L

I

D

D

I

P

A

Y

A

D

L

L

E

L

K

R

F

H

K

V

L

Q

E

E

T

F

G

G

S

G

V

V

V

R

G

G

F

Y

P

P

G

K

S

A

K

E

N

P

I

L

T

P

T

A

E

A

E

D

L

F

L

W

Q

G

L

L

E

P

C

V

D

E

I

F

L

L

V

V

P

P

A

A

L

L

E

E

N

K

Q

Q

I

I

T

T

G

E

E

Q

N

N

A

A

D

W

K

R

I

I

N

R

A

A

K

R

I

I

V

V

A

A

E

E

G

G

A

A

N

N

G

G

P

P

T

T

P

P

E

A

A

A

D

D

N

D

I

I

L

L

A

L

K

E

R

K

N

G

I

V

L

L

V

V

L

V

P

P

D

D

V

V

L

I

A

A

N

N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

D

Q

F

Y

N

G

S

Y

Y

Y

F

W

W

T

T

E

E

V

I

N

N

E

A

R

R

L

L

E

E

R

R

K

V

M

L

V

R

E

N

A

A

F

F

D

E

S

A

V

V

Y

W

S

Q

L

V

Y

A

K

Q

E

E

H

K

G

-

D

K

I

I

N

P

M

L

R

R

G

T

A

A

Y

Y

M

V

V

V

A

A

I

A

K

T

R

R

I

V

Y

L

E

E

A

A

M

R

K

A

I

L

R
|
R

G
|
G

active site: K111 (= K106), D151 (= D146)
binding glutamic acid: A70 (= A65), G77 (= G72), M96 (= M91), K111 (= K106), P150 (= P145), D151 (= D146), D164 (= D159), M168 (≠ R163), S354 (= S350), R417 (= R414), G418 (= G415)

Sites not aligning to the query:

binding glutamic acid: 419, 420

3aoeB Crystal structure of hetero-hexameric glutamate dehydrogenase from thermus thermophilus (leu bound form)
51% identity, 97% coverage: 8:415/421 of query aligns to 16:421/424 of 3aoeB

query
sites
3aoeB

F

W

E

E

I

I

V

F

Q

T

N

E

L

Q

I

V

D

D

D

R

C

V

V

V

E

P

K

Y

L

L

G

G

L

R

D

L

R

A

T

P

V

L

A

A

E

E

I

S

L

L

R

K

E

R

P

P

Q

K

R

R

V

V

L

L

S

I

V

V

S

D

I

V

P

P

V

V

Q

R

M

L

D

D

G

G

S

S

I

V

K

A

V

Y

F

F

K

E

G

G

F

Y

R

R

S

V

Q

H

H

H

N

N

N

T

A
|
A

C

R

G

G

P

P

C

A

K

K

G

G

I

V

R

R

F

Y

H

H

P

P

D

E

V

V

S

T

M

L

D

S

E

E

V

V

K

M

A

A

L

L

S

A

T

G

W

W

M

M

S

T

L

I

K

K

C

N

A

A

V

A

V

V

N

G

L

L

P

P

Y

Y

G

G

K
|
K

G

G

V

I

I

R

V

V

N

D

H

P

K

R

E

K

L

L

S

S

P

P

N

G

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

R

Y

Y

I

T

R

S

A

E

I

I

A

G

P

I

V

L

L

L

G

G

A

P

D

D

M

R

D

D

V

I

P

P

A

A

P

P

D
|
D

V

V

G

N

T

T

N

G

G

E

Q

R

T

E

M

M

G

A

W

W

M

M

D

D

E

T

F

Y

A

S

R

M

Y

N

R

V

G

G

Y

R

N

T

E

V

F

P

G

G

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

A

V

L

G

G

S

S

L

L

G

G

R

R

V

R

E

D

A

A

T

T

S

G

R

R

G

G

C

V

V

F

F

I

T

T

I

A

R

A

Q

A

A

A

K

E

A

K

T

I

G

G

L

L

E

Q

L

V

K

E

D

G

A

A

T

R

V

V

V

A

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

N

I

A

A

A

R

R

L

A

L

F

S

-

R

H

E

D

D

H

G

G

C

A

K

R

V

V

L

V

A

A

V

V

S

Q

D

D

S

H

T

T

G

G

A

T

A

V

Y

Y

D

N

P

E

N

A

G

G

L

I

D

D

I

P

A

Y

A

D

L

L

E

L

K

R

F

H

K

V

L

Q

E

E

T

F

G

G

S

G

V

V

V

R

G

G

F

Y

P

P

G

K

S

A

K

E

N

P

I

L

T

P

T

A

E

A

E

D

L

F

L

W

Q

G

L

L

E

P

C

V

D

E

I

F

L

L

V

V

P

P

A

A

L

L

E

E

N

K

Q

Q

I

I

T

T

G

E

E

Q

N

N

A

A

D

W

K

R

I

I

N

R

A

A

K

R

I

I

V

V

A

A

E

E

G

G

A

A

N

N

G

G

P

P

T

T

P

P

E

A

A

A

D

D

N

D

I

I

L

L

A

L

K

E

R

K

N

G

I

V

L

L

V

V

L

V

P

P

D

D

V

V

L

I

A

A

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

D

Q

F

Y

N

G

S

Y

Y

Y

F

W

W

T

T

E

E

V

I

N

N

E

A

R

R

L

L

E

E

R

R

K

V

M

L

V

R

E

N

A

A

F

F

D

E

S

A

V

V

Y

W

S

Q

L

V

Y

A

K

Q

E

E

H

K

G

-

D

K

I

I

N

P

M

L

R

R

G

T

A

A

Y

Y

M

V

V

V

A

A

I

A

K

T

R

R

I

V

Y

L

E

E

A

A

M
x
R

K

A

I

L

R
|
R

G
|
G

active site: K114 (= K106), D154 (= D146)
binding leucine: A73 (= A65), R417 (≠ M411), R420 (= R414), G421 (= G415)

Sites not aligning to the query:

binding leucine: 423

8owmC Crystal structure of glutamate dehydrogenase 2 from arabidopsis thaliana binding ca, NAD and 2,2-dihydroxyglutarate (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 20:411/413 of 8owmC

query
sites
8owmC

L

L

G

G

L

L

D

D

R

S

T

K

V

I

A

E

E

R

I
x
S

L

L

R

M

E
x
I

P

P

Q

F

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

T

I

L

K

V

V

S

F

Y

K

I

G

G

F

F

R

R

S

V

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K
|
K

G

G

G
|
G

I

I

R

R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M
|
M

S

T

L

W

K
|
K

C

T

A

A

V

V

V

A

N

D

L

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

I

G

V

C

N

S

H

P

K

R

E

D

L

L

S

S

P

L

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

T

D

D

V

V

P

P

A
|
A

P

P

D
|
D

V
x
M

G

G

T

T

N

N

G

A

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R
|
R

V

E

E

A

A

A

T
|
T

S

G

R

R

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

Q

D

G

A

L

T

T

V

F

V

V

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

I

W

A

A

R

K

L

L

L

I

S

-

R

H

E

E

D

K

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

T

G

G

A

A

A

I

Y

R

D

N

P

P

N

E

G

G

L

I

D

D

I

I

A

N

A

A

L

L

E

I

K

K

F

H

K

K

L

D

E

A

T

T

G

G

S

S

V

L

V

N

G

D

F

F

P

N

G

G

S

G

K

D

N

A

I

M

T

N

T

S

E

D

E

E

L

L

Q

I

L

H

E

E

C

C

D

D

I

V

L

L

V

I

P

P

A
x
C

A
|
A

L

L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

G

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

V

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

I

L

Y

A

A

N
|
N

A

A

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

E

E

E

E

K

V

V

N

N

E

L

R

E

L

L

E

Q

R

K

K

Y

M

M

V

T

E

R

A

A

F

F

D

H

S

N

V

I

Y

K

S

T

L

M

Y

C

K

H

E

T

H

H

G

-

D

S

I

C

N

N

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

V

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

Q

I

L

R

R

G

G

W

W

binding calcium ion: S29 (≠ I31), I32 (≠ E34)
binding nicotinamide-adenine-dinucleotide: D144 (= D146), M145 (≠ V147), R183 (= R186), T187 (= T190), F216 (= F219), G217 (= G220), N218 (= N221), V219 (= V222), D239 (= D243), I240 (≠ S244), C290 (≠ A294), A291 (= A295), A313 (= A317), N314 (= N318), N339 (= N343)
binding 2,2-bis(oxidanyl)pentanedioic acid: K68 (= K70), G70 (= G72), M89 (= M91), K92 (= K94), K104 (= K106), A142 (= A144), R183 (= R186), N314 (= N318), V343 (= V347), S346 (= S350)

8s38A Crystal structure of medicago truncatula glutamate dehydrogenase 2 in complex with citrate and NAD (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 14:405/407 of 8s38A

query
sites
8s38A

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I

S

L

L

R

L

E

I

P

P

Q

Y

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

S

I

L

K

V

V

S

F

Y

K

V

G

G

F

F

R

R

S

I

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K

K

G

G

I

I

R

R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M

M

S

T

L

W

K

K

C

T

A

A

V

V

N

D

L

I

P

P

Y

Y

G

G

G

A

K

K

G

G

V

I

I

G

V

C

N

N

H

P

K

K

E

D

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

R

D

D

V

V

P

P

A

A

P

P

D

D

V

M

G

G

T

T

N

N

G

S

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R

R

V

E

E

A

A

A

T
|
T

S

G

R

L

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

A

F

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

S

D

D

A

M

T

T

V

F

V

A

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

I

W

A

A

R

K

L

A

L

I

S

F

R

-

E

E

D

R

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

N

G

G

A

A

A

I

Y

S

D

N

P

P

N

N

G

G

L

I

D

D

I

I

A

A

L

L

E

L

K

K

F

H

K

K

L

A

E

G

T

N

G

G

S

S

V

L

V

K

G

D

F

F

P

S

G

G

S

G

K

D

N

A

I

M

T

N

T

P

E

N

E

D

L

L

Q

V

L

H

E

D

C

C

D

D

I

V

L

L

V

I

P

P

A
x
C

A
|
A

L
|
L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

N

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

V

L

Y

A

A

N
|
N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

D

E

E

E

K

V

V

N

N

E

Q

R

E

L

L

E

K

R

R

K

Y

M

M

V

T

E

K

A

A

F

F

D

N

S

D

V

I

Y

K

S

A

L

N

Y

C

K

K

E

T

H

H

G

-

D

N

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

L

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

L

I

L

R

R

G

G

W

W

binding nicotinamide-adenine-dinucleotide: T181 (= T190), F210 (= F219), G211 (= G220), N212 (= N221), V213 (= V222), D233 (= D243), I234 (≠ S244), C284 (≠ A294), A285 (= A295), L286 (= L296), A307 (= A317), N308 (= N318), N333 (= N343)

8s3bA Glutamate dehydrogenase (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 17:408/410 of 8s3bA

query
sites
8s3bA

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I
x
S

L

L

R

L

E
x
I

P

P

Q

Y

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

S

I

L

K

V

V

S

F

Y

K

V

G

G

F

F

R

R

S

I

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K
|
K

G
|
G

I

I

R

R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M
|
M

S

T

L

W

K

K

C

T

A

A

V

V

N

D

L

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

I

G

V

C

N

N

H

P

K

K

E

D

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

R

D

D

V

V

P

P

A
|
A

P

P

D
|
D

V

M

G

G

T

T

N

N

G

S

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R

R

V

E

E

A

A

A

T
|
T

S

G

R

L

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

A

F

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

S

D

D

A

M

T

T

V

F

V

A

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

I

W

A

A

R

K

L

A

L

I

S

F

R

-

E

E

D

R

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

N

G

G

A

A

A

I

Y

S

D

N

P

P

N

N

G

G

L

I

D

D

I

I

A

A

L

L

E

L

K

K

F

H

K

K

L

A

E

G

T

N

G

G

S

S

V

L

V

K

G

D

F

F

P

S

G

G

S

G

K

D

N

A

I

M

T

N

T

P

E

N

E

D

L

L

Q

V

L

H

E

D

C

C

D

D

I

V

L

L

V

I

P

P

A

C

A
|
A

L

L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

N

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

V

L

Y

A

A

N
|
N

A

A

G

G

G
|
G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

D

E

E

E

K

V

V

N

N

E

Q

R

E

L

L

E

K

R

R

K

Y

M

M

V

T

E

K

A

A

F

F

D

N

S

D

V

I

Y

K

S

A

L

N

Y

C

K

K

E

T

H

H

G

-

D

N

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

L

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

L

I

L

R

R

G

G

W

W

binding 3-(1~{H}-1,2,3,4-tetrazol-5-yl)benzoic acid: K65 (= K70), G66 (= G71), G67 (= G72), M86 (= M91), K101 (= K106), A139 (= A144), D141 (= D146), G339 (= G346), S343 (= S350)
binding calcium ion: S26 (≠ I31), I29 (≠ E34)
binding nicotinamide-adenine-dinucleotide: T184 (= T190), F213 (= F219), G214 (= G220), N215 (= N221), V216 (= V222), D236 (= D243), I237 (≠ S244), A288 (= A295), A310 (= A317), N311 (= N318), N336 (= N343)

8s38B Crystal structure of medicago truncatula glutamate dehydrogenase 2 in complex with citrate and NAD (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 21:412/414 of 8s38B

query
sites
8s38B

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I
x
S

L
|
L

R

L

E
x
I

P

P

Q

Y

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

S

I

L

K

V

V

S

F

Y

K

V

G

G

F

F

R

R

S

I

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K

K

G

G

I

I

R

R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M

M

S

T

L

W

K

K

C

T

A

A

V

V

N

D

L

I

P

P

Y

Y

G

G

G

A

K

K

G

G

V

I

I

G

V

C

N

N

H

P

K

K

E

D

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

R

D

D

V

V

P

P

A

A

P

P

D

D

V

M

G

G

T

T

N

N

G

S

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R

R

V

E

E

A

A

A

T

T

S

G

R

L

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

A

F

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

S

D

D

A

M

T

T

V

F

V

A

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

T

I

W

A

A

R

K

L

A

L

I

S

F

R

-

E

E

D

R

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D

D

S

I

T

N

G

G

A

A

A

I

Y

S

D

N

P

P

N

N

G

G

L

I

D

D

I

I

A

A

L

L

E

L

K

K

F

H

K

K

L

A

E

G

T

N

G

G

S

S

V

L

V

K

G

D

F

F

P

S

G

G

S

G

K

D

N

A

I

M

T

N

T

P

E

N

E

D

L

L

Q

V

L

H

E

D

C

C

D

D

I

V

L

L

V

I

P

P

A

C

A

A

L

L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

N

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A

A

N

N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

V

L

Y

A

A

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

D

E

E

E

K

V

V

N

N

E

Q

R

E

L

L

E

K

R

R

K

Y

M

M

V

T

E

K

A

A

F

F

D

N

S

D

V

I

Y

K

S

A

L

N

Y

C

K

K

E

T

H

H

G

-

D

N

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

L

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

L

I

L

R

R

G

G

W

W

binding calcium ion: S30 (≠ I31), L31 (= L32), I33 (≠ E34)

8s3dA Crystal structure of medicago truncatula glutamate dehydrogenase 2 in complex with 2-amino-2-hydroxyglutarate (reaction intermediate) and NAD (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 19:410/412 of 8s3dA

query
sites
8s3dA

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I
x
S

L

L

R

L

E
x
I

P

P

Q

Y

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

S

I

L

K

V

V

S

F

Y

K

V

G

G

F

F

R

R

S

I

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K
|
K

G
|
G

I

I

R
|
R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M
|
M

S

T

L

W

K
|
K

C

T

A

A

V

V

N

D

L

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

I

G

V

C

N

N

H

P

K

K

E

D

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

R

D

D

V

V

P

P

A
|
A

P
|
P

D
|
D

V
x
M

G

G

T

T

N

N

G

S

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G
|
G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R
|
R

V

E

E

A

A

A

T
|
T

S

G

R

L

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

A

F

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

S

D

D

A

M

T

T

V

F

V

A

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

I

W

A

A

R

K

L

A

L

I

S

F

R

-

E

E

D

R

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

N

G

G

A

A

A

I

Y

S

D

N

P

P

N

N

G

G

L

I

D

D

I

I

A

A

L

L

E

L

K

K

F

H

K

K

L

A

E

G

T

N

G

G

S

S

V

L

V

K

G

D

F

F

P

S

G

G

S

G

K

D

N

A

I

M

T

N

T

P

E

N

E

D

L

L

Q

V

L

H

E

D

C

C

D

D

I

V

L

L

V

I

P

P

A
x
C

A
|
A

L

L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

N

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

V

L

Y

A

A

N
|
N

A

A

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

D

E

E

E

K

V

V

N

N

E

Q

R

E

L

L

E

K

R

R

K

Y

M

M

V

T

E

K

A

A

F

F

D

N

S

D

V

I

Y

K

S

A

L

N

Y

C

K

K

E

T

H

H

G

-

D

N

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

L

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

L

I

L

R

R

G

G

W

W

binding (2S)-2-azanyl-2-oxidanyl-pentanedioic acid: K67 (= K70), G68 (= G71), G69 (= G72), M88 (= M91), K91 (= K94), K103 (= K106), A141 (= A144), R182 (= R186), V342 (= V347), S345 (= S350)
binding calcium ion: S28 (≠ I31), I31 (≠ E34)
binding glycine: P142 (= P145), G171 (= G175), N217 (= N221)
binding nicotinamide-adenine-dinucleotide: R71 (= R74), D143 (= D146), M144 (≠ V147), R182 (= R186), T186 (= T190), F215 (= F219), G216 (= G220), N217 (= N221), V218 (= V222), D238 (= D243), I239 (≠ S244), C289 (≠ A294), A290 (= A295), A312 (= A317), N313 (= N318), N338 (= N343)

8s3aE Crystal structure of medicago truncatula glutamate dehydrogenase 2 in complex with 2,6-pyridinedicarboxylic acid and NAD (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 19:410/412 of 8s3aE

query
sites
8s3aE

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I

S

L

L

R

L

E

I

P

P

Q

Y

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

S

I

L

K

V

V

S

F

Y

K

V

G

G

F

F

R

R

S

I

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K
|
K

G

G

I

I

R
|
R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M
|
M

S

T

L

W

K
|
K

C

T

A

A

V

V

N

D

L

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

I

G

V

C

N

N

H

P

K

K

E

D

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

R

D

D

V

V

P

P

A
|
A

P
|
P

D
|
D

V
x
M

G

G

T

T

N

N

G

S

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R
|
R

V

E

E

A

A

A

T
|
T

S

G

R

L

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

A

F

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

S

D

D

A

M

T

T

V

F

V

A

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

I

W

A

A

R

K

L

A

L

I

S

F

R

-

E

E

D

R

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

N

G

G

A

A

A

I

Y

S

D

N

P

P

N

N

G

G

L

I

D

D

I

I

A

A

L

L

E

L

K

K

F

H

K

K

L

A

E

G

T

N

G

G

S

S

V

L

V

K

G

D

F

F

P

S

G

G

S

G

K

D

N

A

I

M

T

N

T

P

E

N

E

D

L

L

Q

V

L

H

E

D

C

C

D

D

I

V

L

L

V

I

P

P

A
x
C

A
|
A

L

L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

N

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

V

L

Y

A

A

N
|
N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

D

E

E

E

K

V

V

N

N

E

Q

R

E

L

L

E

K

R

R

K

Y

M

M

V

T

E

K

A

A

F

F

D

N

S

D

V

I

Y

K

S

A

L

N

Y

C

K

K

E

T

H

H

G

-

D

N

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

L

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

L

I

L

R

R

G

G

W

W

binding nicotinamide-adenine-dinucleotide: R71 (= R74), D143 (= D146), M144 (≠ V147), R182 (= R186), T186 (= T190), F215 (= F219), G216 (= G220), N217 (= N221), V218 (= V222), D238 (= D243), I239 (≠ S244), C289 (≠ A294), A290 (= A295), A312 (= A317), N313 (= N318), N338 (= N343)
binding pyridine-2,6-dicarboxylic acid: K67 (= K70), M88 (= M91), K91 (= K94), K103 (= K106), A141 (= A144), P142 (= P145), D143 (= D146), R182 (= R186), N313 (= N318), S345 (= S350)

8s39C Crystal structure of medicago truncatula glutamate dehydrogenase 2 in complex with isophthalic acid and NAD (see paper)
48% identity, 94% coverage: 22:416/421 of query aligns to 20:411/413 of 8s39C

query
sites
8s39C

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I

S

L

L

R

L

E

I

P

P

Q

Y

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

S

I

L

K

V

V

S

F

Y

K

V

G

G

F

F

R

R

S

I

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K
|
K

G

G

I

I

R
|
R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M
|
M

S

T

L

W

K
|
K

C

T

A

A

V

V

N

D

L

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

I

G

V

C

N

N

H

P

K

K

E

D

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

R

D

D

V

V

P

P

A
|
A

P
|
P

D
|
D

V
x
M

G

G

T

T

N

N

G

S

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

H

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R
|
R

V

E

E

A

A

A

T
|
T

S

G

R

L

G

G

C

V

V

V

F

F

T

A

I

T

R

E

Q

A

A

L

A

F

K

A

A

E

T

Y

G

G

L

K

E

S

L

I

K

S

D

D

A

M

T

T

V

F

V

A

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

I

W

A

A

R

K

L

A

L

I

S

F

R

-

E

E

D

R

G

G

C

G

K

K

V

V

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

N

G

G

A

A

A

I

Y

S

D

N

P

P

N

N

G

G

L

I

D

D

I

I

A

A

L

L

E

L

K

K

F

H

K

K

L

A

E

G

T

N

G

G

S

S

V

L

V

K

G

D

F

F

P

S

G

G

S

G

K

D

N

A

I

M

T

N

T

P

E

N

E

D

L

L

Q

V

L

H

E

D

C

C

D

D

I

V

L

L

V

I

P

P

A

C

A
|
A

L

L

E

G

N

G

Q

V

I

L

T

N

G

K

E

E

N

N

A

A

D

N

K

D

I

V

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

I

V

I

L

L

P

P

D

D

V

V

L

Y

A

A

N
|
N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

D

E

E

E

K

V

V

N

N

E

Q

R

E

L

L

E

K

R

R

K

Y

M

M

V

T

E

K

A

A

F

F

D

N

S

D

V

I

Y

K

S

A

L

N

Y

C

K

K

E

T

H

H

G

-

D

N

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

L

K

N

R

R

I

V

Y

A

E

R

A

A

M

T

K

L

I

L

R

R

G

G

W

W

binding benzene-1,3-dicarboxylic acid: K68 (= K70), M89 (= M91), K92 (= K94), K104 (= K106), A142 (= A144), P143 (= P145), D144 (= D146), R183 (= R186), N314 (= N318), S346 (= S350)
binding nicotinamide-adenine-dinucleotide: R72 (= R74), D144 (= D146), M145 (≠ V147), R183 (= R186), T187 (= T190), F216 (= F219), G217 (= G220), N218 (= N221), V219 (= V222), D239 (= D243), I240 (≠ S244), A291 (= A295), A313 (= A317), N314 (= N318), N339 (= N343)

P80053 Glutamate dehydrogenase 2; GDH-2; EC 1.4.1.3 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
47% identity, 95% coverage: 20:417/421 of query aligns to 23:420/420 of P80053

query
sites
P80053

E

E

K

L

L

L

G

G

L

L

D

D

R

N

T

E

V

T

A

L

E

E

I

T

L

L

R

S

E

Q

P

P

Q

E

R

R

V

I

L

I

S

Q

V

V

S

K

I

I

P

Q

V

I

Q

R

M

G

D

S

D

D

G

G

S

K

I

L

K

K

V

T

F

F

K

M

G

G

F

W

R

R

S

S

Q

Q

H

H

N

N

N

S

A

A

C

L

G

G

P

P

C

Y

K

K

G

G

I

V

R

R

F

Y

H

H

P

P

D

N

V

V

S

T

M

Q

D

D

E

E

V

V

K

E

A

A

L

L

S

S

T

M

W

I

M

M

S

T

L

W

K

K

C

N

A

S

V

L

N

L

L

L

P

P

Y

Y

G

G

K

K

G

G

V

V

I

R

V

V

N

D

H

P

K

K

E

K

L

L

S

T

P

R

N

E

E

E

L

L

E

E

Q

Q

L

L

C

S

R

R

G

K

Y

Y

I

I

R

Q

A

A

I

I

A

Y

P

K

V

Y

L

L

G

G

A

S

D

E

M

L

D

D

V

I

P

P

A

A

P

P

D

D

V

V

G

N

T

T

N

D

G

S

Q

Q

T

T

M

M

G

A

W

W

M

F

M

L

D

D

E

E

F

Y

A

I

R

K

Y

I

R

T

G

G

Y

K

N

V

E

D

F

F

G

A

V

V

I

F

T

T

G

G

K

K

P

P

I

V

I

E

V

L

G

G

S

I

L

G

G

V

R

R

V

L

E

Y

A

S

T

T

S

G

R

L

G

G

C

V

V

A

F

T

T

I

I

A

R

K

Q

E

A

A

K

N

A

K

T

F

G

I

L

G

E

G

L

V

K

E

D

E

A

A

T

R

V

V

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

Y

I

Y

A

A

A

G

R

K

L

F

L

L

S

S

R

-

E

E

D

M

G

G

C

A

K

K

V

I

L

V

A

G

V

V

S

S

D

D

S

S

T

K

G

G

A

G

A

V

Y

I

D

N

P

E

N
x
K

G

G

L

I

D

D

I

V

A

G

A
x
K

L

A

E

I

K

E

F

I

K

K

L

E

E

K

T

T

G

G

S

S

V

V

V

I

G

N

F

Y

P

P

G

E

S

G

K

R

N

K

I

V

T

T

T

N

E

E

L

L

Q

I

L

S

E

D

C

C

D

D

I

I

L

L

V

I

P

P

A

A

L

L

E

E

N

N

Q

V

I

I

T

N

G

K

E

F

N

N

A

A

D

P

K

K

I

V

N

K

A

A

K

K

I

L

V

I

A

V

E

E

G

G

A

A

N

N

G

G

P

P

T

L

T

T

P

A

E

D

A

A

D

D

N

E

I

I

L

M

A

R

K

Q

R

R

N

G

I

I

L

A

V

V

L

V

P

P

D

D

V

I

L

L

A

A

N

N

A

A

G

G

V

V

T

V

V

G

S

S

Y

Y

F

V

E

E

W

W

V

A

Q

N

N

N

Q

K

Y

M

G

G

Y

E

Y

I

W

I

T

S

E

D

E

E

V

A

N

K

E
x
K

R

L

L

I

E

V

R

D

K

R

M

M

V

N

E

N

A

A

F

F

D

N

S

T

V

L

Y

Y

S

D

L

Y

Y

H

-

Q

K
|
K

E
x
K

H

L

G

E

D

D

I

H

N

D

M

L

R

R

G

T

A

A

Y

M

M

A

V

L

A

A

I

V

K

D

R

R

I

V

Y

V

E

R

A

A

M

M

K

K

I

A

R

R

G

G

W

I

L

L

K254 (≠ N252) modified: N6-methyllysine
K260 (≠ A258) modified: N6-methyllysine
K372 (≠ E370) modified: N6-methyllysine
K391 (= K388) modified: N6-methyllysine
K392 (≠ E389) modified: N6-methyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylmethionine

1v9lA L-glutamate dehydrogenase from pyrobaculum islandicum complexed with NAD (see paper)
47% identity, 97% coverage: 8:417/421 of query aligns to 4:418/418 of 1v9lA

query
sites
1v9lA

F

L

E

E

I

Y

V

V

Q

L

N

N

L

Y

I

V

D

K

C

G

V

V

E

E

K

L

L

G

G

G

L

F

D

P

R

E

T

D

V

F

A

Y

E

K

I

I

L

L

R

S

E

R

P

P

Q

R

R

R

V

V

L

L

S

I

V

V

S

N

I

I

P

P

V

V

Q

R

M

L

D

D

D

G

G

G

S

G

I

F

K

E

V

V

F

F

K

E

G

G

F

Y

R

R

S

V

Q

Q

H

H

N

C

N

D

A

V

C

L

G

G

P

P

C

Y

K

K

G

G

I

V

R

R

F

F

H

H

P

P

D

E

V

V

S

T

M

L

D

A

E

D

V

D

K

V

A

A

L

L

S

A

T

I

W

L

M

M

S

T

L

L

K

K

C

N

A

S

V

L

V

A

N

G

L

L

P

P

Y

Y

G

G

G

A

K
|
K

G

G

G

A

V

V

I

R

V

V

N

D

H

P

K

K

E

K

L

L

S

S

P

Q

N

R

E

E

L

L

E

E

Q

E

L

L

C

S

R

R

G

G

Y

Y

I

A

R

R

A

A

I

I

A

A

P

P

V

L

L

I

G

G

A

D

D

V

M

V

D

D

V

I

P

P

A

A

P

P

D
|
D

V

V

G

G

T

T

N

N

G

A

Q

Q

T

I

M

M

G

A

W

W

M

M

M

V

D

D

E

E

F

Y

A

S

R

K

Y

I

R

K

G

G

Y

Y

N

N

E

V

F

P

G

G

V

V

I

F

T

T

G

S

K

K

P

P

I

P

I

E

V

L

G

W

G

G

S

N

L

P

G

V

R

R

V

E

E

Y

A

A

T
|
T

S

G

R

F

G

G

C

V

V

A

F

V

T

A

I

T

R

R

Q

E

A

M

A

A

K

K

A

K

T

L

G

W

L

G

E

G

L

I

K

E

D

G

A

K

T

T

V

V

V

A

V

I

Q
|
Q

G

G

F

M

G
|
G

N
|
N

V
|
V

G

G

S

R

I

W

A

T

A

A

R

Y

L

W

L

L

S

E

R

K

E

M

D

-

G

G

C

A

K

K

V

V

L

I

A

A

V

V

S

S

D
|
D

S
x
I

T

N

G

G

A

V

A

A

Y

Y

D

R

P

K

N

E

G

G

L

L

D

N

I

V

A

E

A

L

L

I

E

Q

K

K

F

N

K

K

L

G

E

L

T

T

G

G

S

P

-

A

-

L

V

V

V

E

G

L

F

F

P

T

G

T

S

K

K

D

N

N

I

A

-

E

-

F

-

V

-

K

T

N

T

P

E

D

E

A

L

I

L

F

Q

K

L

L

E

D

C

V

D

D

I

I

L

F

V

V

P

P

A

A

A
|
A

L
x
I

E

E

N

N

Q

V

I

I

T

R

G

G

E

D

N

N

A

A

D

G

K

L

I

V

N

K

A

A

K

R

I

L

V

V

A

V

E

E

G

G

A
|
A

N
|
N

G

G

P

P

T

T

P

P

E

E

A

A

D

E

N

R

I

I

L

L

A

Y

K

E

R

R

N

G

I

V

L

V

V

V

L

V

P

P

D

D

V

I

L

L

A

A

N
|
N

A

A

G

G

V

V

T

I

V

M

S

S

Y

Y

F

L

E

E

W

W

V

V

Q

E

N

N

Q

L

Y

Q

G

W

Y

Y

Y

I

W

W

T

D

E

E

V

T

N

R

E

K

R

R

L

L

E

E

R

N

K

I

M

M

V

V

E

N

A

N

F

V

D

E

S

R

V

V

Y

Y

S

K

L

R

Y

W

K

Q

E

R

H

E

G

K

D

G

I

W

N

T

M

M

R

R

G

D

A

A

Y

I

M

V

V

T

A

A

I

L

K

E

R

R

I

I

Y

Y

E

N

A

A

M

M

K

K

I

I

R

R

G

G

W

W

L

I

active site: K102 (= K106), D142 (= D146)
binding nicotinamide-adenine-dinucleotide: T186 (= T190), Q213 (= Q217), G216 (= G220), N217 (= N221), V218 (= V222), D238 (= D243), I239 (≠ S244), A296 (= A295), I297 (≠ L296), A318 (= A317), N319 (= N318), N344 (= N343)

6yeiA Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD (see paper)
47% identity, 94% coverage: 22:416/421 of query aligns to 17:408/409 of 6yeiA

query
sites
6yeiA

L

L

G

G

L

L

D

D

R

S

T

K

V

L

A

E

E

K

I
x
S

L
|
L

R

L

E
x
I

P
|
P

Q

F

R

R

V

E

L

I

S

K

V

V

S

E

I

C

P

T

V

I

Q

P

M

K

D

D

G

G

S

T

I

L

K

A

V

S

F

F

K

V

G

G

F

F

R

R

S

V

Q

Q

H

H

N

D

N

N

A

A

C

R

G

G

P

P

C

M

K

K

G

G

I

I

R

R

F

Y

H

H

P

P

D

E

V

V

S

D

M

P

D

D

E

E

V

V

K

N

A

A

L

L

S

A

T

Q

W

L

M

M

S

T

L

W

K

K

C

T

A

A

V

V

V

A

N

K

L

I

P

P

Y

Y

G

G

G

A

K

K

G

G

V

I

I

G

V

C

N

D

H

P

K

S

E

K

L

L

S

S

P

I

N

S

E

E

L

L

E

E

Q

R

L

L

C

T

R

R

G

V

Y

F

I

T

R

Q

A

K

I

I

A

H

P

D

V

L

L

I

G

G

A

I

D

H

M

T

D

D

V

V

P

P

A

A

P

P

D

D

V

M

G

G

T

T

N

G

G

P

Q

Q

T

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

F

Y

A

S

R

K

Y

F

R

H

G

G

Y

Y

N

S

E

P

F

-

G

A

V

V

I

V

T

T

G

G

K

K

P

P

I

I

I

D

V

L

G

G

S

S

L

L

G

G

R

R

V

D

E

A

A

A

T
|
T

S

G

R

R

G

G

C

V

V

M

F

F

T

G

I

T

R

E

Q

A

A

L

K

N

A

E

T

H

G

G

L

K

E

T

L

I

K

S

D

G

A

Q

T

R

V

F

V

V

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

S

I

W

A

A

R

K

L

L

L

I

S

S

R

-

E

E

D

K

G

G

C

G

K

K

V

I

L

V

A

A

V

V

S

S

D
|
D

S
x
I

T

T

G

G

A

A

A

I

Y

K

D

N

P

K

N

D

G

G

L

I

D

D

I

I

A

P

A

A

L

L

E

L

K

K

F

H

K

T

L

K

E

E

T

H

G

R

S

G

V

V

V

K

G

G

F

F

P

D

G

G

S

A

K

D

N

P

I

I

T

D

T

P

E

N

E

S

L

I

L

L

Q

V

L

E

E

D

C

C

D

D

I

I

L

L

V

V

P

P

A

A

A
|
A

L
|
L

E

G

N

G

Q

V

I

I

T

N

G

R

E

E

N

N

A

A

D

N

K

E

I

I

N

K

A

A

K

K

I

F

V

I

A

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

P

P

E

D

A

A

D

D

N

E

I

I

L

L

A

S

K

K

R

K

N

G

I

V

L

V

V

I

L

L

P

P

D

D

V

I

L

Y

A

A

N
|
N

A

S

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

Q

I

Y

Q

G

G

Y

F

Y

M

W

W

T

E

E

E

E

K

V

V

N

N

E

D

R

E

L

L

E

K

R

T

K

Y

M

M

V

T

E

R

A

S

F

F

D

K

S

D

V

L

Y

K

S

E

L

M

Y

C

K

K

E

T

H

H

G

-

D

S

I

C

N

D

M

L

R

R

G

M

A

G

A

A

Y

F

M

T

V

L

A

G

I

V

K

N

R

R

I

V

Y

A

E

Q

A

A

M

T

K

I

I

L

R

R

G

G

W

W

binding potassium ion: S26 (≠ I31), L27 (= L32), I29 (≠ E34), P30 (= P35)
binding nicotinamide-adenine-dinucleotide: T184 (= T190), F213 (= F219), G214 (= G220), N215 (= N221), V216 (= V222), D236 (= D243), I237 (≠ S244), A288 (= A295), L289 (= L296), A310 (= A317), N311 (= N318), N336 (= N343)

Query Sequence

>WP_092481889.1 NCBI__GCF_900115975.1:WP_092481889.1
MSQEINCFEIVQNLIDDCVEKLGLDRTVAEILREPQRVLSVSIPVQMDDGSIKVFKGFRS
QHNNACGPCKGGIRFHPDVSMDEVKALSTWMSLKCAVVNLPYGGGKGGVIVNHKELSPNE
LEQLCRGYIRAIAPVLGADMDVPAPDVGTNGQTMGWMMDEFARYRGYNEFGVITGKPIIV
GGSLGRVEATSRGCVFTIRQAAKATGLELKDATVVVQGFGNVGSIAARLLSREDGCKVLA
VSDSTGAAYDPNGLDIAALEKFKLETGSVVGFPGSKNITTEELLQLECDILVPAALENQI
TGENADKINAKIVAEGANGPTTPEADNILAKRNILVLPDVLANAGGVTVSYFEWVQNQYG
YYWTEEEVNERLERKMVEAFDSVYSLYKEHGDINMRGAAYMVAIKRIYEAMKIRGWLGNK
A

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory