SitesBLAST
Comparing WP_092481907.1 NCBI__GCF_900115975.1:WP_092481907.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
C3UVB0 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 from Desulfococcus multivorans (see paper)
54% identity, 100% coverage: 1:385/385 of query aligns to 1:389/389 of C3UVB0
- A80 (= A77) mutation to E: Loses the FAD cofactor and dehydrogenase activity.
- R87 (= R84) binding substrate
- V88 (≠ L85) mutation to S: A residual dehydrogenase activity is observed.
- N91 (= N88) binding substrate
- FGIT 126:129 (≠ FAIT 123:126) binding FAD
- S135 (= S132) binding FAD; binding substrate
- WIS 159:161 (= WIS 156:158) binding FAD
- S181 (≠ V178) binding substrate
- R271 (= R268) binding FAD
- FQMN 281:284 (= FQMN 278:281) binding FAD
- R340 (≠ K336) binding FAD
- A344 (≠ S340) binding FAD
- V366 (= V362) mutation to Y: Loses the FAD cofactor but a residual dehydrogenase activity is observed.
- EGSAN 367:371 (≠ EGTSN 363:367) binding FAD
- R385 (= R381) binding substrate
3mpiC Structure of the glutaryl-coenzyme a dehydrogenase glutaryl-coa complex (see paper)
54% identity, 100% coverage: 1:385/385 of query aligns to 1:389/395 of 3mpiC
- active site: I128 (= I125), T129 (= T126), T245 (= T242), E367 (= E363), L379 (≠ Q375)
- binding flavin-adenine dinucleotide: I128 (= I125), T129 (= T126), G134 (= G131), S135 (= S132), W159 (= W156), I160 (= I157), S161 (= S158), M365 (≠ I361), V366 (= V362), S369 (≠ T365), N371 (= N367), M375 (≠ F371)
- binding glutaryl-coenzyme A: R87 (= R84), F126 (= F123), S135 (= S132), V137 (= V134), S181 (≠ V178), F239 (≠ M236), R246 (= R243), N315 (≠ H312), V366 (= V362), E367 (= E363), G368 (= G364), I376 (= I372), R385 (= R381), R389 (= R385)
3mpjB Structure of the glutaryl-coenzyme a dehydrogenase (see paper)
54% identity, 100% coverage: 1:385/385 of query aligns to 1:389/393 of 3mpjB
- active site: I128 (= I125), T129 (= T126), T245 (= T242), E367 (= E363), L379 (≠ Q375)
- binding flavin-adenine dinucleotide: F126 (= F123), I128 (= I125), T129 (= T126), G134 (= G131), S135 (= S132), W159 (= W156), I160 (= I157), S161 (= S158), V366 (= V362), S369 (≠ T365), N371 (= N367), M375 (≠ F371)
- binding : H36 (= H35), F37 (≠ R36), Y39 (≠ R38), A164 (≠ T161), Q165 (≠ V162), D167 (= D164), N193 (≠ S190)
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
43% identity, 98% coverage: 1:378/385 of query aligns to 1:378/380 of 4l1fA
- active site: L125 (≠ I125), T126 (= T126), G242 (≠ T242), E363 (= E363), R375 (≠ Q375)
- binding coenzyme a persulfide: T132 (≠ S132), H179 (≠ K179), F232 (= F232), M236 (= M236), E237 (= E237), L239 (= L239), D240 (≠ N240), R243 (= R243), Y362 (≠ V362), E363 (= E363), G364 (= G364), R375 (≠ Q375)
- binding flavin-adenine dinucleotide: F123 (= F123), L125 (≠ I125), T126 (= T126), G131 (= G131), T132 (≠ S132), F156 (≠ W156), I157 (= I157), T158 (≠ S158), R268 (= R268), Q270 (= Q270), F271 (= F271), I275 (= I275), F278 (= F278), L281 (≠ N281), Q336 (≠ K336), I337 (= I337), G340 (≠ S340), I358 (≠ S358), Y362 (≠ V362), T365 (= T365), Q367 (≠ N367)
- binding 1,3-propandiol: L5 (= L5), Q10 (≠ L10)
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
42% identity, 94% coverage: 19:378/385 of query aligns to 18:377/378 of 5ol2F
- active site: L124 (≠ I125), T125 (= T126), G241 (≠ T242), G374 (≠ Q375)
- binding calcium ion: E29 (= E30), E33 (≠ A34), R35 (= R36)
- binding coenzyme a persulfide: L238 (= L239), R242 (= R243), E362 (= E363), G363 (= G364)
- binding flavin-adenine dinucleotide: F122 (= F123), L124 (≠ I125), T125 (= T126), P127 (≠ A128), T131 (≠ S132), F155 (≠ W156), I156 (= I157), T157 (≠ S158), E198 (≠ T199), R267 (= R268), F270 (= F271), L274 (≠ I275), F277 (= F278), Q335 (≠ K336), L336 (≠ I337), G338 (= G339), G339 (≠ S340), Y361 (≠ V362), T364 (= T365), E366 (≠ N367)
P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
41% identity, 98% coverage: 4:381/385 of query aligns to 33:411/412 of P15651
- 152:161 (vs. 123:132, 50% identical) binding FAD
- S161 (= S132) binding substrate
- WIT 185:187 (≠ WIS 156:158) binding FAD
- DMGR 269:272 (≠ NDTR 240:243) binding substrate
- R297 (= R268) binding FAD
- QILGG 365:369 (≠ KIYGS 336:340) binding FAD
- E392 (= E363) active site, Proton acceptor
- TSE 394:396 (≠ TSN 365:367) binding FAD
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
41% identity, 98% coverage: 4:381/385 of query aligns to 6:384/384 of 1jqiA
- active site: G377 (≠ Q375)
- binding acetoacetyl-coenzyme a: L95 (vs. gap), F125 (= F123), S134 (= S132), F234 (= F232), M238 (= M236), Q239 (≠ E237), L241 (= L239), D242 (≠ N240), R245 (= R243), Y364 (≠ V362), E365 (= E363), G366 (= G364)
- binding flavin-adenine dinucleotide: F125 (= F123), L127 (≠ I125), S128 (≠ T126), G133 (= G131), S134 (= S132), W158 (= W156), T160 (≠ S158), R270 (= R268), F273 (= F271), L280 (≠ F278), Q338 (≠ K336), I339 (= I337), G342 (≠ S340), I360 (≠ S358), T367 (= T365), E369 (≠ N367), I370 (= I368)
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
40% identity, 98% coverage: 1:378/385 of query aligns to 1:377/379 of 1ukwB
- active site: L124 (≠ I125), S125 (≠ T126), T241 (= T242), E362 (= E363), R374 (≠ Q375)
- binding cobalt (ii) ion: D145 (≠ E146), H146 (≠ N147)
- binding flavin-adenine dinucleotide: F122 (= F123), L124 (≠ I125), S125 (≠ T126), G130 (= G131), S131 (= S132), W155 (= W156), S157 (= S158), K200 (= K201), L357 (≠ S358), Y361 (≠ V362), E362 (= E363), T364 (= T365), E366 (≠ N367), L370 (≠ F371)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
40% identity, 98% coverage: 1:378/385 of query aligns to 1:377/379 of 1ukwA
- active site: L124 (≠ I125), S125 (≠ T126), T241 (= T242), E362 (= E363), R374 (≠ Q375)
- binding flavin-adenine dinucleotide: F122 (= F123), L124 (≠ I125), S125 (≠ T126), G130 (= G131), S131 (= S132), W155 (= W156), S157 (= S158), L357 (≠ S358), Y361 (≠ V362), E362 (= E363), T364 (= T365), E366 (≠ N367), L370 (≠ F371)
5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
41% identity, 98% coverage: 1:379/385 of query aligns to 1:374/374 of 5lnxD
- active site: L122 (≠ I125), T123 (= T126), G239 (≠ T242), E358 (= E363), K370 (≠ Q375)
- binding flavin-adenine dinucleotide: L122 (≠ I125), T123 (= T126), G128 (= G131), S129 (= S132), F153 (≠ W156), T155 (≠ S158), R265 (= R268), Q267 (= Q270), F268 (= F271), I272 (= I275), N275 (≠ F278), I278 (≠ N281), Q331 (≠ K336), I332 (= I337), G335 (≠ S340), Y357 (≠ V362), T360 (= T365), E362 (≠ N367)
7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
40% identity, 98% coverage: 4:381/385 of query aligns to 9:387/387 of 7y0aC
- binding flavin-adenine dinucleotide: F128 (= F123), L130 (≠ I125), S131 (≠ T126), G136 (= G131), S137 (= S132), W161 (= W156), T163 (≠ S158), T214 (≠ V209), R273 (= R268), F276 (= F271), L280 (≠ I275), L283 (≠ F278), V285 (≠ M280), Q341 (