SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_092482150.1 NCBI__GCF_900115975.1:WP_092482150.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
49% identity, 98% coverage: 3:248/251 of query aligns to 2:253/271 of Q72J02

query
sites
Q72J02

K

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C192 (= C187) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
46% identity, 99% coverage: 2:249/251 of query aligns to 62:311/453 of Q9VLJ8

query
sites
Q9VLJ8

S
x
T

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T62 (≠ S2) modified: Phosphothreonine

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
49% identity, 98% coverage: 3:248/251 of query aligns to 4:249/270 of D4GSF3

query
sites
D4GSF3

K

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C188 (= C187) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 99% coverage: 1:248/251 of query aligns to 1:248/249 of P12282

query
sites
P12282

M

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R14 (= R14) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S44) mutation to A: No effect.
R73 (= R73) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G128) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D130) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C142) mutation to A: No effect.
C172 (= C171) mutation to A: No zinc bound and no enzyme activity.
C175 (= C174) mutation to A: No zinc bound and no enzyme activity.
C187 (= C187) mutation to A: No effect.
C231 (≠ M231) mutation to A: No effect.
C244 (= C244) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C247) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
41% identity, 98% coverage: 2:248/251 of query aligns to 1:240/240 of 1jwbB

query
sites
1jwbB

S

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K

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A

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N

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K

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A

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M

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G

G

Q

Q

A

-

L

I

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T

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T

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Y

Q

Q

G

D

-

G

-

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P

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C

L

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C

C

I

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K

R

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-

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-

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-

D

G

R

E

L

A

G

G

V

V

L

M

G

A

A

P

V

L

P

I

G

G

I

V

I

I

G

G

S

S

L

L

E

Q

A

A

T

M

E

E

A

A

V

I

K

K

V

M

I

L

L

A

G

G

T

Y

G

G

E

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

T

M

Y

Y

D

D

A

A

L

M

S

T

M

C

R

Q

F

F

Y

R

T

E

V

M

E

K

I

L

N

M

R

R

D

N

P

P

Q

G

C
|
C

A

E

V

V

C
|
C

G

G

active site: R13 (= R14), D129 (= D130)
binding adenosine monophosphate: G37 (= G38), G39 (= G40), G40 (= G41), D61 (= D62), F62 (≠ G63), K85 (= K86), L108 (≠ R109), C127 (≠ G128), T128 (= T129), D129 (= D130), N130 (= N131), V133 (≠ T134)
binding zinc ion: C171 (= C171), C236 (= C244), C239 (= C247)

1jw9B Structure of the native moeb-moad protein complex (see paper)
41% identity, 98% coverage: 2:248/251 of query aligns to 1:240/240 of 1jw9B

query
sites
1jw9B

S

A

K

E

L

L

S

S

S

D

E

Q

Q

E

L

M

E

L

R

R

Y

Y

K

N

R
|
R

N

Q

I

I

L

I

L

L

D

R

G

G

V

F

G

D

S

F

R

D

G

G

Q

Q

E

E

K

A

L

L

L

K

Q

D

A

S

R

R

V

V

L

L

V

I

V

V

G

G

A

L

G

G

L

L

G

G

S

C

P

A

A

A

A

S

F

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

H

N

I

L

G

T

L

L

V

L

D

D

G

F

D

D

R

T

V

V

D

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

T

L

L

H

H

A

S

T

D

P

A

D

T

L

V

G

G

R

Q

H

P

K

K

I

V

D

E

S

S

A

A

R

R

D

D

K

A

L

L

T

T

A

R

L

I

N

N

P

P

D

H

I

I

R

A

V

I

T

T

G

P

Y

V

E

N

Q

A

R

L

L

L

N

D

E

D

D

A

N

E

F

L

R

A

E

A

I

L

I

I

A

A

G

E

Y

H

D

D

L

L

V

V

V

L

D

D

G

C

T

T

D
|
D

N

N

F

V

G

A

T

V

R

R

F

N

L

Q

I

L

N

N

R

A

A

G

C

C

V

F

E

A

Q

A

R

K

K

V

P

P

Y

L

I

V

F

S

G

G

G

A

V

A

L

I

G

R

Y

M

T

E

G

G

Q

Q

A

-

L

I

T

T

I

V

L

F

P

T

G

Y

Q

Q

G

D

-

G

-

E

P

P

C
|
C

L

Y

A

R

C

C

I

L

F

S

K

R

D

L

V

F

P

-

E

-

D

-

A

-

P

-

S

-

C

-

D

G

R

E

L

A

G

G

V

V

L

M

G

A

A

P

V

L

P

I

G

G

I

V

I

I

G

G

S

S

L

L

E

Q

A

A

T

M

E

E

A

A

V

I

K

K

V

M

I

L

L

A

G

G

T

Y

G

G

E

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

T

M

Y

Y

D

D

A

A

L

M

S

T

M

C

R

Q

F

F

Y

R

T

E

V

M

E

K

I

L

N

M

R

R

D

N

P

P

Q

G

C
|
C

A

E

V

V

C
|
C

G

G

active site: R13 (= R14), D129 (= D130)
binding zinc ion: C171 (= C171), C236 (= C244), C239 (= C247)

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
43% identity, 98% coverage: 4:249/251 of query aligns to 61:320/482 of O59954

query
sites
O59954

L

L

S

H

S

G

E

E

Q

E

L

Y

E

R

R

R

Y

Y

K

G

R

R

N

Q

I

M

L

I

L

V

D

P

G

Q

V

F

G

G

S

L

R

Q

G
|
G

Q

Q

E

L

K

K

L

L

L

R

Q

D

A

A

R

K

V

V

L

L

V

I

V

V

G

G

A

A

G

G

L

L

G
|
G

S

C

P

P

A

A

F

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

T

I

I

G

G

L

L

V

V

D

D

G

G

D

D

R

T

V

V

D

E

L

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

I

V

L

L

H

H

A

R

T

S

P

R

D

N

L

V

G

G

R

K

H

L

K

K

I

V

D

D

S

S

A

A

R

I

D

E

K

Y

L

L

T

R

A

E

L

L

N

N

P

P

D

H

I

P

R

T

V

Y

T

I

G

A

Y

H

E

Q

Q

A

R

H

L

L

N

T

E

P

D

R

N

E

F

A

R

P

E

D

I

I

I

F

A

K

G

D

Y

Y

D

D

L

L

V

I

V

L

D

D

G
x
C

T

T

D

D

N

N

F

P

G

A

T

T

R

R

F

Y

L

L

I

I

N

S

R

D

A

T

C

A

V

V

E

L

Q

L

R

G

K

K

P

P

Y

L

I

V

F

S

G

A

G

S

V

A

L

L

G

R

Y

T

T
x
E

G

G

Q

Q

A

L

L

M

T

V

I

L

-

N

-

P

-

Q

L

P

P

P

G

G

Q

D

-

K

-

T

-

G

G

G

P

P

C

C

L

Y

A

R

C

C

I

V

F

F

K

P

D

K

V

P

P

P

E

A

D

N

A

S

-

V

P

T

S

S

C

C

D

A

R

D

L

G

G

G

V

I

L

L

G

G

A

P

V

V

P

V

G
|
G

I

T

I

M

G

G

S

V

L

L

E

Q

A

A

T

S

E

E

A

A

V

I

K

K

V

V

I

L

L

T

G

S

T

A

G

G

E

E

P

S

L

V

L

E

G

A

-

T

-

P

R

S

L

L

T

I

Y

F

D

S

A

A

L

Y

S

S

M

S

-

P

R

Q

F

F

Y

R

T

T

V

I

E

K

I

L

-

R

N

S

R

R

D

R

P

P

Q

N

C

C

A

A

V

V

C

C

G

S

A

A

G82 (= G25) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G43) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R73) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G128) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ T158) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G198) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

1zfnA Structural analysis of escherichia coli thif (see paper)
42% identity, 98% coverage: 4:248/251 of query aligns to 1:244/244 of 1zfnA

query
sites
1zfnA

L

M

S

N

S

D

E

R

Q

D

L

F

E

M

R

R

Y

Y

K

S

R
|
R

N

Q

I

I

L

L

D

D

G

D

V

I

G

A

S

L

R

D

G

G

Q

Q

E

Q

K

K

L

L

Q

D

A

S

R

Q

V

V

L

L

V

I

V
x
I

G
|
G

A

L

G
|
G

L

L

G

G

S

T

P

P

A

A

F

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

T

I

L

G

V

L

L

V

A

D
|
D

G

D

D

D

R

D

V

V

D

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

I

I

L

L

H

F

A

T

T

T

P

E

D

D

L

I

G

D

R

R

H

P

K
|
K

I

S

D

Q

S

V

A

S

R

Q

D

Q

K

R

L

L

T

T

A

Q

L

L

N

N

P

P

D

D

I

I

R

Q

V

L

T

T

G

A

Y

L

E

Q

Q

Q

R

R

L

L

N

T

E

G

D

E

N

A

F

L

R

K

E

D

I

A

I

V

A

A

G

R

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

T
|
T

D
|
D

N

N

F

M

G

A

T
|
T

R

R

F

Q

L

E

I

I

N

N

R

A

A

A

C

C

V

V

E

A

Q

L

R

N

K

T

P

P

Y

L

I

I

F

T

G

A

G

S

V

A

L

V

G

G

Y

F

T

G

G

G

Q

Q

A

L

L

M

T

V

I

L

L

T

-

P

P

P

G

W

Q

E

G

Q

P

G

C
|
C

L

Y

A

R

C
|
C

I

L

F

W

K

P

D

D

V

-

P

N

P

Q

E

E

D

P

A

E

P

R

S

N

C

C

D

R

R

T

L

A

G

G

V

V

L

V

G

G

A

P

V

V

P

V

G

G

I

V

I

M

G

G

S

T

L

L

E

Q

A

A

T

L

E

E

A

A

V

I

K

K

V

L

I

L

L

S

G

G

T

I

G

E

E

T

P

P

L

-

L

A

G

G

R

E

L

L

L

R

T

L

Y

F

D

D

A

G

L

K

S

S

M

S

R

Q

F

W

Y

R

T

S

V

L

E

A

I

L

N

R

R

R

D

A

P

S

Q

G

C
|
C

A

P

V

V

C
|
C

G

G

active site: R11 (= R14), D127 (= D130)
binding adenosine-5'-triphosphate: I34 (≠ V37), G35 (= G38), G37 (= G40), G38 (= G41), D59 (= D62), R70 (= R73), Q71 (= Q74), K83 (= K86), T126 (= T129), D127 (= D130), T131 (= T134)
binding zinc ion: C169 (= C171), C172 (= C174), C240 (= C244), C243 (= C247)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 98% coverage: 4:248/251 of query aligns to 1:244/251 of P30138

query
sites
P30138

L

M

S

N

S

D

E

R

Q

D

L

F

E

M

R

R

Y

Y

K

S

R

R

N

Q

I

I

L

L

D

D

G

D

V

I

G

A

S

L

R

D

G

G

Q

Q

E

Q

K

K

L

L

Q

D

A

S

R

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

A

A

F

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

T

I

L

G

V

L

L

V

A

D

D

G

D

D

D

R

D

V

V

D

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

L

L

H

F

A

T

T

T

P

E

D

D

L

I

G

D

R

R

H

P

K

K

I

S

D

Q

S

V

A

S

R

Q

D

Q

K

R

L

L

T

T

A

Q

L

L

N

N

P

P

D

D

I

I

R

Q

V

L

T

T

G

A

Y

L

E

Q

Q

Q

R

R

L

L

N

T

E

G

D

E

N

A

F

L

R

K

E

D

I

A

I

V

A

A

G

R

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

T

T

D

D

N

N

F

M

G

A

T

T

R

R

F

Q

L

E

I

I

N

N

R

A

A

A

C

C

V

V

E

A

Q

L

R

N

K

T

P

P

Y

L

I

I

F

T

G

A

G

S

V

A

L

V

G

G

Y

F

T

G

G

G

Q

Q

A

L

L

M

T

V

I

L

L

T

-

P

P

P

G

W

Q

E

G

Q

P

G

C
|
C

L

Y

A

R

C
|
C

I

L

F
x
W

K

P

D

D

V

-

P

N

P

Q

E

E

D

P

A

E

P

R

S

N

C
|
C

D

R

R

T

L

A

G

G

V

V

L

V

G

G

A

P

V

V

P

V

G

G

I

V

I

M

G

G

S

T

L

L

E

Q

A

A

T

L

E

E

A

A

V

I

K

K

V

L

I

L

L

S

G

G

T

I

G

E

E

T

P

P

L

-

L

A

G

G

R

E

L

L

L

R

T

L

Y

F

D

D

A

G

L

K

S

S

M

S

R

Q

F

W

Y

R

T

S

V

L

E

A

I

L

N

R

R

R

D

A

P

S

Q

G

C
|
C

A

P

V

V

C
|
C

G

G

C169 (= C171) binding Zn(2+)
C172 (= C174) binding Zn(2+)
W174 (≠ F176) mutation to A: No adenylation of ThiS.
C184 (= C187) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C244) binding Zn(2+)
C243 (= C247) binding Zn(2+)

1zud3 Structure of this-thif protein complex (see paper)
42% identity, 98% coverage: 4:248/251 of query aligns to 1:239/240 of 1zud3

query
sites
1zud3

L

M

S

N

S

D

E

R

Q

D

L

F

E

M

R

R

Y

Y

K

S

R
|
R

N

Q

I

I

L
|
L

L

L

D
|
D

G

D

V

I

G

A

S

L

R

D

G

G

Q

Q

E

Q

K

K

L

L

Q

D

A

S

R

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

A

A

F

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

T

I

L

G

V

L

L

V

A

D

D

G

D

D

D

R

D

V

V

D

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

L

L

H

F

A

T

T

T

P

E

D

D

L

I

G

D

R

R

H

P

K

K

I

S

D

Q

S

V

A

S

R

Q

D

Q

K

R

L

L

T

T

A

Q

L

L

N

N

P

P

D

D

I

I

R

Q

V

L

T

T

G

A

Y

L

E

Q

Q

Q

R

R

L

L

N

T

E

G

D

E

N

A

F

L

R

K

E

D

I

A

I

V

A

A

G

R

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

T

T

D
|
D

N

N

F

M

G

A

T

T

R

R

F

Q

L

E

I

I

N

N

R

A

A

A

C

C

V

V

E

A

Q

L

R

N

K

T

P

P

Y

L

I

I

F

T

G

A

G

S

V

A

L

V

G

G

Y

F

T

G

G

G

Q

Q

A

L

L

M

T

V

I

L

L

T

-

P

P

P

G

W

Q

E

G

Q

P

G

C
|
C

L

Y

A

R

C

C

I

L

F

W

K

-

D

-

V

-

P

P

P

D

E

N

D

Q

A

E

P

P

S

T

C

A

D

-

R

-

L

-

G

G

V

V

L

V

G

G

A

P

V

V

P

V

G

G

I

V

I

M

G

G

S

T

L

L

E

Q

A

A

T

L

E

E

A

A

V

I

K

K

V

L

I

L

L

S

G

G

T

I

G

E

E

T

P

P

L

-

L

A

G

G

R

E

L

L

L

R

T

L

Y

F

D

D

A

G

L

K

S

S

M

S

R

Q

F

W

Y

R

T

S

V

L

E

A

I

L

N

R

R

R

D

A

P

S

Q

G

C
|
C

A

P

V

V

C
|
C

G

G

active site: R11 (= R14), D127 (= D130)
binding calcium ion: L14 (= L17), D16 (= D19)
binding zinc ion: C169 (= C171), C235 (= C244), C238 (= C247)

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
42% identity, 98% coverage: 4:248/251 of query aligns to 39:290/440 of P38820

query
sites
P38820

L

L

S

S

S

L

E

E

Q

E

L

Y

E

Q

R

R

Y

Y

K

G

R

R

N

Q

I

M

L

I

L

V

D

E

G

E

V

T

G

G

S

G

-

V

R

A

G

G

Q

Q

E

V

K

K

L

L

L

K

Q

N

A

T

R

K

V

V

L

L

V

V

G

G

A

A

G

G

L

L

G

G

S

C

P

P

A

A

A

L

F

P

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

Q

I

I

G

G

L

I

V

V

D

D

G

N

D

D

R

V

V

V

D

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

L

L

H

H

A

D

T

S

P

S

D

R

L

V

G

G

R

M

H

L

K

K

I

C

D

E

S

S

A

A

R

R

D

Q

K

Y

L

I

T

T

A

K

L

L

N

N

P

P

D

H

I

I

R

N

V

V

T

V

G

T

Y

Y

E

P

Q

V

R

R

L

L

N

N

E

S

D

S

N

N

F

A

R

F

E

D

I

I

I

F

A

K

G

G

Y

Y

D

N

L

Y

V

I

V

L

D

D

G

C

T

T

D

D

N

S

F

P

G

L

T

T

R

R

F

Y

L

L

I

V

N

S

R

D

A

V

C

A

V

V

E

N

Q

L

R

G

K

I

P

T

Y

V

I

V

F

S

G

A

G

S

V

G

L

L

G

G

Y

T

T

E

G

G

Q

Q

A

-

L

L

T

T

I

I

L

L

P

N

G

F

Q

N

-

N

-

I

G

G

P

P

C

C

L

Y

A

R

C

C

I

F

F

Y

K

P

D

T

V

P

P

P

E

P

D

N

A

A

-

V

P

T

S

S

C
|
C

D

Q

R

E

L

G

G

G

V

V

L

I

G

G

A

P

V

C

P

I

G

G

I

L

I

V

G

G

S

T

L

M

E

M

A

A

T

V

E

E

A

T

V

L

K

K

V

L

I

I

L

L

G

G

-

I

-

Y

T

T

G

N

E

E

P

N

L

F

L

S

G

P

R

F

L

L

L

M

T

L

Y

Y

D

S

A

G

L

F

-

P

-

Q

S

S

M

L

R

R

F

T

Y

F

T

K

V

M

E

R

I

-

N

G

R

R

D

Q

P

E

Q

K

C

C

A

L

V

C

C

C

G

G

C225 (= C187) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
38% identity, 95% coverage: 2:240/251 of query aligns to 1:217/217 of 1jwaB

query
sites
1jwaB

S

A

K

E

L

L

S

S

S

D

E

Q

Q

E

L

M

E

L

R

R

Y

Y

K

N

R
|
R

N

Q

I

I

L

I

L

L

D

R

G

G

V

F

G

D

S

F

R

D

G

G

Q

Q

E

E

K

A

L

L

L

K

Q

D

A

S

R

R

V

V

L

L

V

I

V

V

G

G

A

L

G
|
G

L

L

G

G

S

C

P

A

A

A

A

S

F

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

H

N

I

L

G

T

L

L

V

L

D
|
D

G
x
F

D

D

R

T

V

V

D

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

L

L

H

H

A

S

T

D

P

A

D

T

L

V

G

G

R

Q

H

P

K
|
K

I

V

D

E

S

S

A

A

R

R

D

D

K

A

L

L

T

T

A

R

L

I

N

N

P

P

D

H

I

I

R

A

V

I

T

T

G

P

Y

V

E

N

Q

A

R
x
L

L

L

N

D

E

D

D

A

N

E

F

L

R

A

E

A

I

L

I

I

A

A

G

E

Y

H

D

D

L

L

V

V

V

L

D

D

G

C

T

T

D
|
D

N
|
N

F

V

G

A

T
x
V

R

R

F

N

L

Q

I

L

N

N

R

A

A

G

C

C

V

F

E

A

Q

A

R

K

K

V

P

P

Y

L

I

V

F

S

G

G

G

A

V

A

L

I

G

R

Y

M

T

E

G

G

Q

Q

A

-

L

I

T

T

I

V

L

F

P

T

G

Y

Q

E

G

A

P

-

C

-

L

-

A

-

C

-

I

-

F

-

K

-

D

-

V

-

P

-

E

-

D

-

A

-

P

-

S

-

C

-

D

-

R

-

L

-

G

G

V

V

L

M

G

A

A

P

V

L

P

I

G

G

I

V

I

I

G

G

S

S

L

L

E

Q

A

A

T

M

E

E

A

A

V

I

K

K

V

M

I

L

L

A

G

G

T

Y

G

G

E

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

T

M

Y

Y

D

D

A

A

L

M

S

T

M

C

R

Q

F

F

Y

R

T

E

V

M

E

K

I

L

N

M

R

R

active site: R13 (= R14), D129 (= D130)
binding adenosine-5'-triphosphate: G39 (= G40), G40 (= G41), D61 (= D62), F62 (≠ G63), R72 (= R73), K85 (= K86), L108 (≠ R109), D129 (= D130), N130 (= N131), V133 (≠ T134)

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 84% coverage: 4:213/251 of query aligns to 6:214/423 of 6yubA

query
sites
6yubA

L

L

S

S

S

K

E

E

Q

E

L

I

E

E

R

R

Y

Y

K

S

R

R

N

Q

I

M

L

I

L

V

D

P

G

G

V

M

G

G

S

K

R

E

G

G

Q

Q

E

L

K

R

L

L

L

M

Q

N

A

A

R

K

V

V

L

L

V

I

G

G

A

A

G

G

L

L

G

G

S

C

P

P

A

A

F

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

T

I

I

G

G

L

I

V

V

D

D

G

G

D

D

R

S

V

V

D

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

L

A

H

H

A

A

T

T

P

K

D

R

L

V

G

G

R

M

H

L

K

K

I

V

D

D

S

S

A

L

R

I

D

T

K

H

L

L

T

I

A

E

L

I

N

N

P

P

D

L

I

P

R

V

V

Y

T

V

G

P

Y

Y

E

R

Q

F

R

D

L

L

N

T

E

P

D

Q

N

N

F

A

R

A

E

Q

I

I

A

K

G

P

Y

W

D

D

L

V

V

I

V

L

D

D

G

C

T

T

D

D

N

N

F

P

G

A

T

T

R

R

F

Y

L

L

I

I

N

S

R

D

A

V

C

C

V

V

E

L

Q

L

R

G

K

K

P

P

Y

L

I

V

F

S

G

A

V

S

L

V

G

Q

Y

K

T

S

G

G

Q

Q

A

L

L

I

-

V

-

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

T

V

I

V

L

N

P

K

G

K

Q

A

G

A

P

P

C
|
C

L

Y

A

R

C
|
C

I

C

F

F

K

K

D

-

V

-

P

-

E

-

D

-

A

-

P

-

S

-

C

-

D

-

R

K

L

P

G

G

V

I

L

M

G

G

A

P

V

V

P

V

G

G

I

M

G

G

S

V

L

A

E

Q

A

A

T

G

E

E

A

A

V

I

K

K

V

I

I

L

L

V

binding zinc ion: C183 (= C171), C186 (= C174)

Sites not aligning to the query:

binding zinc ion: 266, 269

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 84% coverage: 4:213/251 of query aligns to 7:213/289 of 6yubB

query
sites
6yubB

L

L

S

S

S

K

E

E

Q

E

L

I

E

E

R

R

Y

Y

K

S

R

R

N

Q

I

M

L

I

L

V

D

P

G

G

V

M

G

G

S

K

R

E

G

G

Q

Q

E

L

K

R

L

L

L

M

Q

N

A

A

R

K

V

V

L

L

V

I

G

G

A

A

G

G

L

L

G

G

S

C

P

P

A

A

F

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

H

T

I

I

G

G

L

I

V

V

D

D

G

G

D

D

R

S

V

V

D

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

L

A

H

H

A

A

T

T

P

K

D

R

L

V

G

G

R

M

H

L

K

K

I

V

D

D

S

S

A

L

R

I

D

T

K

H

L

L

T

I

A

E

L

I

N

N

P

P

D

L

I

P

R

V

V

Y

T

V

G

P

Y

Y

E

R

Q

F

R

D

L

L

N

T

E

P

D

Q

N

N

F

A

R

A

E

Q

I

I

A

K

G

P

Y

W

D

D

L

V

V

I

V

L

D

D

G

C

T

T

D

D

N

N

F

P

G

A

T

T

R

R

F

Y

L

L

I

I

N

S

R

D

A

V

C

C

V

V

E

L

Q

L

R

G

K

K

P

P

Y

L

I

V

F

S

G

A

V

S

L

V

G

Q

Y

K

T

S

G

G

Q

Q

A

L

L

I

-

V

-

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

T

V

I

V

L

N

P

K

G

K

Q

A

G

A

P

P

C
|
C

L

Y

A

R

C
|
C

I

C

F

F

K

K

D

-

V

-

P

-

E

-

D

-

A

-

P

-

S

-

C

-

D

-

R

-

L

-

G

G

V

I

L

M

G

G

A

P

V

V

P

V

G

G

I

M

G

G

S

V

L

A

E

Q

A

A

T

G

E

E

A

A

V

I

K

K

V

I

I

L

L

V

binding zinc ion: C184 (= C171), C187 (= C174)

Sites not aligning to the query:

binding zinc ion: 265

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
42% identity, 98% coverage: 2:248/251 of query aligns to 53:301/460 of O95396

query
sites
O95396

S

A

K

A

L

L

S

S

S

R

E

D

Q

E

L

I

E

L

R

R

Y

Y

K

S

R

R

N

Q

I

L

L

V

L

L

D

P

G

E

V

L

G

G

S

V

R

H

G

G

Q

Q

E

L

K

R

L

L

L

G

Q

T

A

A

R

C

V

V

L

L

V

I

V

V

G

G

A

C

G

G

L

L

G

G

S

C

P

P

A

L

A

A

F

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

H

R

I

L

G

G

L

L

V

V

D

D

G

Y

D

D

R

V

V

V

D

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

I

V

L

L

H

H

A

G

T

E

P

A

D

L

L

A

G

G

R

Q

H

A

K

K

I

A

D

F

S

S

A

A

R

A

D

A

K

S

L

L

T

R

A

R

L

L

N

N

P

S

D

A

I

V

R

E

V

C

T

V

G

P

Y

Y

E
x
T

Q
|
Q

R
x
A

L
|
L

N
x
T

E
x
P

D
x
A

N
x
T

F
x
A

R
x
L

E
x
D

I
x
L

I
x
V

A
x
R

G
x
R

Y
|
Y

D
|
D

L
x
V

V
|
V

V
x
A

D
|
D

G
x
C

T
x
S

D
|
D

N
|
N

F
x
V

G
x
P

T
|
T

R
|
R

F
x
Y

L
|
L

I
x
V

N
|
N

R
x
D

A
|
A

C
|
C

V
|
V

E
x
L

Q
x
A

R
x
G

K
x
R

P
|
P

Y
x
L

I
x
V

F
x
S

G
x
A

G
x
S

V
x
A

L
|
L

G
x
R

Y
x
F

T
x
E

G
|
G

Q
|
Q

A
x
I

L
x
T

T
x
V

I
x
Y

L
x
H

P
x
Y

G
x
D

Q
x
G

G
|
G

P
|
P

C
|
C

L
x
Y

A
x
R

C
|
C

I
|
I

F
|
F

K
x
P

D
x
Q

V
x
P

P
|
P

-
x
A

E
|
E

D
x
T

A
x
V

P
x
T

S
x
N

C
|
C

D

A

R

D

L

G

G

G

V

V

L

L

G

G

A

V

V

V

P

T

G

G

I

V

I

L

G

G

S

C

L

L

E

Q

A

A

T

L

E

E

A

V

V

L

K

K

V

I

I

A

L

A

G

G

T

L

G

G

E

P

P

S

L

Y

L

S

G

G

R

S

L

L

T

L

Y

F

D

D

A

A

L

L

S

R

M

G

R

H

F

F

-

R

Y

S

T

I

V

R

E

L

I

R

N

S

R

R

D

R

P

L

Q

D

C

C

A

A

V

A

C

C

G

G

158:238 (vs. 107:186, 35% identical) Interaction with NFS1
C239 (= C187) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
28% identity, 97% coverage: 5:248/251 of query aligns to 85:338/339 of 3h9jA

query
sites
3h9jA

S

N

S

S

E

T

Q

E

L

N

E

N

R

R

Y

Y

K

S

R

R

N

N

I

F

L

L

-

H

L

Y

D

Q

G

S

V

Y

G

G

S

A

R

N

G

P

-

V

-

L

-

V

Q

Q

E

D

K

K

L

L

L

K

Q

N

A

A

R

K

V

V

L

V

V

I

V

L

G

G

A

C

G
|
G

L

I

G

G

S

N

P

H

A

V

A

S

F

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

H

E

I

I

G

I

L

L

V

I

D
|
D

G

N

D
|
D

R

Q

V

I

D

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

L

L

H

F

A

S

T

E

P

D

D

D

L

V

G

G

R

K

H

N

K
|
K

I

T

D

E

S

V

A

I

R

K

D

R

K

E

L

L

T

L

A

K

L

R

N

N

P

S

D

E

I

I

R

S

V

V

T

S

G

E

Y

I

E

A

Q

L

R
x
N

L
x
I

N

N

E

D

D

Y

N

T

F

D

R

L

E

H

I

K

I

V

A

P

G

E

Y

A

D

D

L

I

V

W

V

V

D

V

G

S

T
x
A

D
|
D

N
x
H

-

P

F

F

G

N

T

L

R

I

F

N

L

W

I

V

N

N

R

K

A

Y

C

C

V

V

E

R

Q

A

R

N

K

Q

P

P

Y

Y

I

I

F

N

G

A

G

G

V

Y

L

V

G

N

Y

D

T

I

G

A

Q

V

-

F

A

G

L

P

T

L

I

Y

L

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

A

E

C

C

I

Q

F

K

K

K

-

E

-

N

-

I

D

D

V

H

P

K

P

I

E

K

D

L

A

I

P

N

S

S

C

R

D

F

R

K

L

P

G

A

V

T

L

F

G

A

A

P

V

V

P

N

G

N

I

V

I

A

G

A

S

A

L

L

E

C

A

A

T

A

E

D

A

V

V

I

K

K

V

F

I

I

L

G

G

K

T

Y

G

S

E

E

P

P

L

L

-

S

L

L

G

N

R

K

L
x
R

L

I

T
x
G

Y

I

D

W

A

S

L

D

S

E

M

I

R

K

F

I

Y

H

T

S

V

Q

E

N

I

M

N

G

R

R

D

S

P

P

Q

V

C
|
C

A

S

V

V

C
|
C

G

G

active site: R157 (= R73)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G40), G125 (= G41), D146 (= D62), D148 (= D64), R157 (= R73), K170 (= K86), N193 (≠ R109), I194 (≠ L110), A213 (≠ T129), D214 (= D130), H215 (≠ N131)
binding zinc ion: C257 (= C171), C334 (= C244), C337 (= C247)
binding : R313 (≠ L223), G315 (≠ T225)

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
28% identity, 97% coverage: 5:248/251 of query aligns to 85:338/338 of 3h5nA

query
sites
3h5nA

S

N

S

S

E

T

Q

E

L

N

E

N

R

R

Y

Y

K

S

R

R

N

N

I

F

L

L

-

H

L

Y

D

Q

G

S

V

Y

G

G

S

A

R

N

G

P

-

V

-

L

-

V

Q

Q

E

D

K

K

L

L

L

K

Q

N

A

A

R

K

V

V

L

V

V

I

V

L

G

G

A

C

G
|
G

G

G

L

I

G

G

S

N

P

H

A

V

A

S

F

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

H

E

I

I

G

I

L

L

V

I

D
|
D

G

N

D

D

R

Q

V

I

D

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

I

V

L

L

H

F

A

S

T

E

P

D

D

D

L

V

G

G

R

K

H

N

K
|
K

I

T

D

E

S

V

A

I

R

K

D

R

K

E

L

L

T

L

A

K

L

R

N

N

P

S

D

E

I

I

R

S

V

V

T

S

G

E

Y

I

E

A

Q

L

R
x
N

L
x
I

N

N

E

D

D

Y

N

T

F

D

R

L

E

H

I

K

I

V

A

P

G

E

Y

A

D

D

L

I

V

W

V

V

D

V

G

S

T
x
A

D
|
D

N
x
H

-

P

F

F

G

N

T

L

R

I

F

N

L

W

I

V

N

N

R

K

A

Y

C

C

V

V

E

R

Q

A

R

N

K

Q

P

P

Y

Y

I

I

F

N

G

A

G

G

V

Y

L

V

G

N

Y

D

T

I

G

A

Q

V

-

F

A

G

L

P

T

L

I

Y

L

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

A

E

C
|
C

I

Q

F

K

K

K

-

E

-

N

-

I

D

D

V

H

P

K

P

I

E

K

D

L

A

I

P

N

S

S

C

R

D

F

R

K

L

P

G

A

V

T

L

F

G

A

A

P

V

V

P

N

G

N

I

V

I

A

G

A

S

A

L

L

E

C

A

A

T

A

E

D

A

V

V

I

K

K

V

F

I

I

L

G

G

K

T

Y

G

S

E

E

P

P

L

L

-

S

L

L

G

N

R

K

L

R

L

I

T

G

Y

I

D

W

A

S

L

D

S

E

M

I

R

K

F

I

Y

H

T

S

V

Q

E

N

I

M

N

G

R

R

D

S

P

P

Q

V

C
|
C

A

S

V

V

C
|
C

G

G

active site: R157 (= R73)
binding adenosine-5'-triphosphate: G124 (= G40), D146 (= D62), N154 (= N70), R157 (= R73), Q158 (= Q74), K170 (= K86), N193 (≠ R109), I194 (≠ L110), A213 (≠ T129), D214 (= D130), H215 (≠ N131)
binding zinc ion: C257 (= C171), C260 (= C174), C334 (= C244), C337 (= C247)

3h5aC Crystal structure of e. Coli mccb (see paper)
26% identity, 97% coverage: 5:248/251 of query aligns to 85:347/358 of 3h5aC

query
sites
3h5aC

S

N

S

S

E

T

Q

E

L

N

E

N

R

R

Y

Y

K

S

R

R

N

N

I

F

L

L

-

H

L

Y

D

Q

G

S

V

Y

G

G

S

A

R

N

G

P

-

V

-

L

-

V

Q

Q

E

D

K

K

L

L

L

K

Q

N

A

A

R

K

V

V

L

V

V

I

V

L

G

G

A

C

G

G

L

I

G

G

S

N

P

H

A

V

A

S

F

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

H

E

I

I

G

I

L

L

V

I

D

D

G

N

D

D

R

Q

V

I

D

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

L

L

H

F

A

S

T

E

P

D

D

D

L

V

G

G

R

K

H

N

K

K

I

T

D

E

S

V

A

I

R

K

D

R

K

E

L

L

T

L

A

K

L

R

N

N

P

S

D

E

I

I

R

S

V

V

T

S

G

E

Y

I

E

A

Q

L

R

N

L

I

N

N

E

D

D

Y

N

T

F

D

R

L

E

H

I

K

I

V

A

P

G

E

Y

A

D

D

L

I

V

W

V

V

D

V

G

S

T

A

D

D

N

H

-

P

F

F

G

N

T

L

R

I

F

N

L

W

I

V

N

N

R

K

A

Y

C

C

V

V

E

R

Q

A

R

N

K

Q

P

P

Y

Y

I

I

F

N

G

A

G

G

V

Y

L

V

G

N

Y

D

T

I

G

A

Q

V

-

F

A

G

L

P

T

L

I

Y

L

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

A

E

C
|
C

-

Q

-

K

I

V

F

V

K

A

D

D

V

L

P

Y

P

G

E

S

D

E

A

K

P

E

S

N

C

I

D

D

-

H

-

K

-

I

-

K

-

L

-

I

-

N

-

S

-

R

-

F

R

K

L

P

G

A

V

T

L

F

G

A

A

P

V

V

P

N

G

N

I

V

I

A

G

A

S

A

L

L

E

C

A

A

T

A

E

D

A

V

V

I

K

K

V

F

I

I

L

G

G

K

T

Y

G

S

E

E

P

P

L

L

-

S

L

L

G

N

R

K

L

R