SitesBLAST
Comparing WP_092482220.1 NCBI__GCF_900115975.1:WP_092482220.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
43% identity, 97% coverage: 1:260/269 of query aligns to 1:260/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ L6), A7 (≠ V7), K8 (= K8), D38 (= D38), T61 (≠ S61), M62 (= M62), A93 (= A93), D94 (= D94), T95 (= T95), T98 (= T98), L102 (= L102), G118 (= G118), Q120 (≠ R120), G124 (= G124), T126 (= T126), A127 (≠ G127), V129 (≠ T129), T223 (= T223)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
40% identity, 96% coverage: 1:259/269 of query aligns to 1:257/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ L6), I7 (≠ V7), K8 (= K8), D38 (= D38), M61 (= M62), A92 (= A93), D93 (= D94), T94 (= T95), T97 (= T98), A115 (≠ C117), G116 (= G118), A119 (= A121), G122 (= G124), T124 (= T126), A125 (≠ G127), V127 (≠ T129)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
40% identity, 96% coverage: 3:260/269 of query aligns to 4:262/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ L6), K9 (= K8), D39 (= D38), M62 (= M62), D94 (= D94), V95 (≠ T95), L102 (= L102), G118 (= G118), Q120 (≠ R120), T121 (≠ A121), T126 (= T126), A127 (≠ G127), V129 (≠ T129)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
39% identity, 96% coverage: 1:259/269 of query aligns to 1:259/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ L6), V7 (= V7), K8 (= K8), Y37 (≠ L37), D38 (= D38), Y40 (≠ N40), T60 (≠ S61), M61 (= M62), A92 (= A93), D93 (= D94), T94 (= T95), T97 (= T98), M101 (≠ L102), C116 (= C117), G117 (= G118), Q119 (≠ R120), A120 (= A121), G123 (= G124), T125 (= T126), A126 (≠ G127), V128 (≠ T129)
- binding nicotinamide-adenine-dinucleotide: M61 (= M62), G62 (= G63), P63 (= P64), S85 (= S86), D86 (≠ S87), G91 (= G92)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
39% identity, 96% coverage: 1:259/269 of query aligns to 1:259/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ L6), V7 (= V7), K8 (= K8), Y37 (≠ L37), D38 (= D38), Y40 (≠ N40), M61 (= M62), A92 (= A93), D93 (= D94), T94 (= T95), T97 (= T98), C116 (= C117), G117 (= G118), Q119 (≠ R120), A120 (= A121), G123 (= G124), T125 (= T126), A126 (≠ G127), V128 (≠ T129)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
40% identity, 84% coverage: 2:226/269 of query aligns to 3:233/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ L6), I8 (≠ V7), K9 (= K8), D39 (= D38), M62 (= M62), A93 (= A93), D94 (= D94), T95 (= T95), T98 (= T98), L102 (= L102), T121 (≠ C117), G122 (= G118), A125 (= A121), G128 (= G124), T130 (= T126), G131 (= G127), V133 (≠ T129)
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
32% identity, 77% coverage: 34:239/269 of query aligns to 34:242/261 of 1o96A
- binding adenosine monophosphate: D38 (= D38), V63 (≠ M62), V99 (≠ T98), A117 (≠ C117), G118 (= G118), Q120 (≠ R120), S121 (≠ A121), Y126 (≠ T126), A127 (≠ G127), T129 (= T129)
- binding flavin-adenine dinucleotide: Q120 (≠ R120), Q182 (≠ V179)
Sites not aligning to the query:
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
32% identity, 77% coverage: 34:239/269 of query aligns to 35:243/264 of P53570
Sites not aligning to the query:
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
29% identity, 78% coverage: 3:211/269 of query aligns to 7:216/255 of Q2TBV3
- K200 (≠ Q195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ E198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
29% identity, 78% coverage: 3:211/269 of query aligns to 7:216/255 of Q9DCW4
- K200 (≠ Q195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ E198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
30% identity, 78% coverage: 3:211/269 of query aligns to 4:213/252 of 2a1uB
- binding adenosine monophosphate: A6 (= A5), V7 (≠ L6), K8 (≠ V7), C39 (≠ D38), C63 (≠ M62), V101 (≠ T98), L119 (≠ C117), G120 (= G118), Q122 (≠ R120), A123 (= A121), D126 (≠ G124), C128 (≠ T126), N129 (≠ G127), Q130 (= Q128), T131 (= T129)
- binding flavin-adenine dinucleotide: P37 (= P36), L182 (≠ K180)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
30% identity, 78% coverage: 3:211/269 of query aligns to 7:216/255 of P38117
- A9 (= A5) binding AMP
- NPFC 39:42 (≠ NPLD 35:38) binding AMP
- C66 (≠ M62) binding AMP
- 123:134 (vs. 118:129, 42% identical) binding AMP
- D128 (= D123) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ N149) to M: in dbSNP:rs1130426
- R164 (= R159) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (≠ I160) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 178:200, 26% identical) Recognition loop
- L195 (= L190) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (≠ Q195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (≠ QAR 195:197) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (≠ QARE 195:198) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (≠ E198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
33% identity, 61% coverage: 34:198/269 of query aligns to 35:200/246 of 1efpB
- binding adenosine monophosphate: D39 (= D38), I63 (≠ M62), V101 (≠ T98), A119 (≠ C117), G120 (= G118), Q122 (≠ R120), A123 (= A121), N126 (≠ G124), M128 (≠ T126), N129 (≠ G127), T131 (= T129)
- binding flavin-adenine dinucleotide: L182 (≠ K180)
Sites not aligning to the query:
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
23% identity, 97% coverage: 1:260/269 of query aligns to 1:249/251 of 5ow0B
- binding adenosine monophosphate: L6 (= L6), A7 (≠ V7), K8 (= K8), D39 (= D38), Y62 (≠ M62), I95 (≠ T98), A114 (≠ C117), G115 (= G118), Q117 (≠ R120), S118 (≠ A121), R123 (≠ T126), G124 (= G127), V125 (≠ Q128), V126 (≠ T129), P127 (≠ G130)
- binding flavin-adenine dinucleotide: L37 (≠ P36), Y38 (≠ L37)
Query Sequence
>WP_092482220.1 NCBI__GCF_900115975.1:WP_092482220.1
MNIVALVKQVPGTDNVKMNPETGTMVRTGKDNVINPLDENALTEALRIKQQTTGTTVTAV
SMGPQTAEKALKEAIALGADEGILLSSRAFAGADTIATARVLAAAVKKLENVSMILCGER
ATDGETGQTGPMVAAMLGIPVVTFVKEINVGENTAIVIRIVENGYEKVEIPYPFMATVVK
DINEPGFPTLSGKVQARETLVKVWSEEELGLPKNELGLTGSPTRVVKIFSPKLARDTRIL
KHDGSGNAVGILVKLLQEKKRNCIKGCGK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory