SitesBLAST
Comparing WP_092482437.1 NCBI__GCF_900115975.1:WP_092482437.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8ipsB Cryo-em structure of heme transporter cyddc from escherichia coli in the inward facing heme loading state (see paper)
38% identity, 99% coverage: 2:570/576 of query aligns to 9:582/588 of 8ipsB
7zdfD If(heme/confined) conformation of cyddc in amp-pnp(cydd) bound state (dataset-4) (see paper)
38% identity, 99% coverage: 2:570/576 of query aligns to 7:580/586 of 7zdfD
- binding phosphoaminophosphonic acid-adenylate ester: S357 (≠ F345), L363 (= L353), S383 (= S372), G384 (= G373), S385 (≠ A374), G386 (= G375), K387 (= K376), S388 (= S377), S389 (≠ T378), Q428 (= Q418)
- binding {3,3'-[(9S)-8,13-diethenyl-3,7,12,17-tetramethyl-9,10-dihydroporphyrin-2,18-diyl-kappa~4~N~21~,N~22~,N~23~,N~24~]dipropanoato(2-)}iron: N189 (≠ S184), F193 (≠ W188), F245 (= F240), S248 (≠ A243), G306 (= G293), T307 (≠ S294), Y309 (≠ F296), H310 (= H297)
- binding magnesium ion: S388 (= S377), Q428 (= Q418)
7zdcD If(heme/confined) conformation of cyddc in adp(cydd) bound state (dataset-3) (see paper)
38% identity, 99% coverage: 2:570/576 of query aligns to 7:580/586 of 7zdcD
- binding adenosine-5'-diphosphate: S357 (≠ F345), K361 (≠ A349), L363 (= L353), G384 (= G373), S385 (≠ A374), G386 (= G375), K387 (= K376), S388 (= S377), S389 (≠ T378)
- binding heme b/c: N189 (≠ S184), N192 (≠ Q187), F193 (≠ W188), F245 (= F240), G306 (= G293), T307 (≠ S294), Y309 (≠ F296), H310 (= H297)
- binding magnesium ion: S388 (= S377), Q428 (= Q418)
P29018 Glutathione/L-cysteine transport system ATP-binding/permease protein CydD; EC 7.4.2.- from Escherichia coli (strain K12) (see paper)
38% identity, 99% coverage: 2:570/576 of query aligns to 9:582/588 of P29018
- R210 (≠ Q203) mutation to G: Exhibits significantly lower levels of cytochrome d than the wild-type; when associated with G-216.; mutation to K: Does not affect cytochrome d levels; when associated with K-216.
- R216 (≠ K209) mutation to G: Exhibits significantly lower levels of cytochrome d than the wild-type; when associated with G-210.; mutation to K: Does not affect cytochrome d levels; when associated with K-210.
- R238 (≠ T231) mutation to G: Exhibits significantly lower levels of cytochrome d than the wild-type; when associated with G-244.; mutation to H: Does not affect cytochrome d levels; when associated with H-244.
- R244 (= R237) mutation to G: Exhibits significantly lower levels of cytochrome d than the wild-type; when associated with G-238.; mutation to H: Does not affect cytochrome d levels; when associated with H-238.
7zdeD If(apo/as isolated) conformation of cyddc in amp-pnp(cydd) bound state (dataset-4) (see paper)
38% identity, 99% coverage: 2:570/576 of query aligns to 8:581/587 of 7zdeD
7zdaD If(apo/asym) conformation of cyddc in adp+pi(cydc)/atp(cydd) bound state (dataset-2) (see paper)
38% identity, 99% coverage: 2:570/576 of query aligns to 8:581/587 of 7zdaD
7zdbD If(heme/bound) conformation of cyddc in adp+pi(cydc)/atp(cydd) bound state (dataset-2) (see paper)
38% identity, 96% coverage: 2:555/576 of query aligns to 9:567/568 of 7zdbD
2onjA Structure of the multidrug abc transporter sav1866 from s. Aureus in complex with amp-pnp (see paper)
32% identity, 87% coverage: 66:568/576 of query aligns to 71:572/578 of 2onjA
- binding phosphoaminophosphonic acid-adenylate ester: Y349 (≠ F345), I356 (≠ A352), S376 (= S372), G377 (= G373), G378 (≠ A374), G379 (= G375), K380 (= K376), S381 (= S377), T382 (= T378), Q422 (= Q418), K477 (≠ R473), S479 (= S475), G480 (= G476), E503 (= E499), H534 (= H530)
2hydA Multidrug abc transporter sav1866 (see paper)
32% identity, 87% coverage: 66:568/576 of query aligns to 71:572/578 of 2hydA
6qv0B Structure of atp-bound outward-facing tm287/288 in complex with sybody sb_tm35 (see paper)
30% identity, 87% coverage: 72:571/576 of query aligns to 72:568/570 of 6qv0B
- binding adenosine-5'-triphosphate: Y343 (≠ F345), V349 (≠ A352), T369 (≠ S372), S371 (≠ A374), G372 (= G375), K373 (= K376), T374 (≠ S377), T375 (= T378), Q415 (= Q418), D470 (≠ R473), S472 (= S475), G474 (= G477), Q475 (= Q478), H527 (= H530)
- binding magnesium ion: T374 (≠ S377), Q415 (= Q418)
6quzD Structure of atpgs-bound outward-facing tm287/288 in complex with sybody sb_tm35 (see paper)
30% identity, 87% coverage: 72:571/576 of query aligns to 76:572/574 of 6quzD
- binding phosphothiophosphoric acid-adenylate ester: Y347 (≠ F345), V353 (≠ A352), G374 (= G373), S375 (≠ A374), G376 (= G375), K377 (= K376), T378 (≠ S377), T379 (= T378), Q419 (= Q418), D474 (≠ R473), L475 (= L474), S476 (= S475), G478 (= G477), Q479 (= Q478), H531 (= H530)
- binding magnesium ion: T378 (≠ S377), Q419 (= Q418)
8vsiA Mechanistic insights revealed by ybtpq in the occluded state (see paper)
32% identity, 89% coverage: 56:568/576 of query aligns to 53:563/571 of 8vsiA
- binding adp orthovanadate: Y339 (≠ F345), S367 (= S372), G368 (= G373), A369 (= A374), G370 (= G375), K371 (= K376), S372 (= S377), T373 (= T378), R376 (≠ H381), Q413 (= Q418), V467 (≠ I472), F468 (≠ R473), S470 (= S475), G472 (= G477), F498 (≠ G503), H525 (= H530)
- binding magnesium ion: S372 (= S377), Q413 (= Q418)
6p6jA Structure of ybtpq importer with substrate ybt-fe bound (see paper)
32% identity, 89% coverage: 56:568/576 of query aligns to 49:559/571 of 6p6jA
9csiA Lipid A export ATP-binding/permease protein MsbA (see paper)
32% identity, 89% coverage: 57:568/576 of query aligns to 59:567/569 of 9csiA
- binding 3,3'-[(1,4-dioxobutane-1,4-diyl)bis(azanediyl)]bis[(4-butylbenzene-1-sulfonamido)benzoic acid]: I65 (≠ L63), R72 (≠ A70), M253 (≠ A250), M257 (≠ T254), T283 (≠ L280), A285 (= A282), G286 (≠ P283)
- binding phosphoaminophosphonic acid-adenylate ester: Y344 (≠ F345), D346 (= D347), T348 (≠ A349), S370 (= S372), G371 (= G373), G373 (= G375), K374 (= K376), T375 (≠ S377), S376 (≠ T378), Q416 (= Q418)
- binding magnesium ion: T375 (≠ S377), Q416 (= Q418)
Sites not aligning to the query:
7metA A. Baumannii msba in complex with tbt1 decoupler (see paper)
32% identity, 89% coverage: 57:568/576 of query aligns to 53:561/564 of 7metA
Sites not aligning to the query:
8gk7A Msba bound to cerastecin c (see paper)
32% identity, 89% coverage: 57:568/576 of query aligns to 54:562/564 of 8gk7A
- binding phosphoaminophosphonic acid-adenylate ester: Y339 (≠ F345), T343 (≠ A349), S365 (= S372), G366 (= G373), G368 (= G375), K369 (= K376), T370 (≠ S377), N467 (≠ R473), L468 (= L474), S469 (= S475), G470 (= G476), G471 (= G477)
- binding 2-[(4-butylbenzene-1-sulfonyl)amino]-5-[(3-{4-[(4-butylbenzene-1-sulfonyl)amino]-3-carboxyanilino}-3-oxopropyl)carbamoyl]benzoic acid: I60 (≠ L63), R67 (≠ A70), M248 (≠ A250), M252 (≠ T254), I277 (≠ L279), T278 (≠ L280), A280 (= A282), G281 (≠ P283), S284 (≠ Y286)
Sites not aligning to the query:
6bppA E. Coli msba in complex with lps and inhibitor g092 (see paper)
29% identity, 90% coverage: 49:568/576 of query aligns to 54:572/576 of 6bppA
- binding (2E)-3-{6-[(1S)-1-(3-amino-2,6-dichlorophenyl)ethoxy]-4-cyclopropylquinolin-3-yl}prop-2-enoic acid: L168 (= L162), A172 (= A166), V175 (≠ I169), S176 (≠ P170), I179 (≠ M173), A256 (= A250), M288 (≠ A282), L291 (≠ F285), M292 (≠ Y286), K296 (≠ R290)
6bplA E. Coli msba in complex with lps and inhibitor g907 (see paper)
29% identity, 90% coverage: 49:568/576 of query aligns to 54:572/576 of 6bplA
- binding (2E)-3-{6-[(1S)-1-(2-chloro-6-cyclopropylphenyl)ethoxy]-4-cyclopropylquinolin-3-yl}prop-2-enoic acid: F154 (≠ A146), L168 (= L162), V175 (≠ I169), S176 (≠ P170), I179 (≠ M173), A256 (= A250), M288 (≠ A282), L291 (≠ F285), M292 (≠ Y286), L295 (= L289), K296 (≠ R290)
7ph2A Nanodisc reconstituted msba in complex with nanobodies, spin-labeled at position a60c (see paper)
29% identity, 90% coverage: 49:568/576 of query aligns to 46:564/569 of 7ph2A
- binding (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-4-[(3~{R})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{R})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{S},5~{S},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanylnonanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid: F277 (≠ L279), A282 (≠ E284), R285 (≠ L287)
Sites not aligning to the query:
- binding (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-4-[(3~{R})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{R})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{S},5~{S},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanylnonanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid: 30, 37
7ph3A Amp-pnp bound nanodisc reconstituted msba with nanobodies, spin- labeled at position a60c (see paper)
29% identity, 90% coverage: 49:568/576 of query aligns to 55:573/577 of 7ph3A
- binding phosphoaminophosphonic acid-adenylate ester: Y349 (≠ F345), S376 (= S372), G377 (= G373), G379 (= G375), K380 (= K376), S381 (= S377), T382 (= T378), Q422 (= Q418), L478 (≠ R473), S480 (= S475), G482 (= G477), Q483 (= Q478), H535 (= H530)
- binding magnesium ion: S381 (= S377), Q422 (= Q418)
Query Sequence
>WP_092482437.1 NCBI__GCF_900115975.1:WP_092482437.1
MMDKRLMREAGAVRSFLALSVGLGLGTGLLAILQASYLARVVNRVSLEGQSLSGVWPWLY
PLLGVIIFRAGLTWMGEVVAHRAAAQIKHALRQRLLAHLLALGPVYAGGERTGELVNALV
EGIEALDAYFTRYLPQLALAALVPLAVLGFVFPLDTISGLILLFTAPLIPLFMFLIGRWA
ESLSQRQWETLSRMSAHFLDVLQGLTTLKIFGRSKSQAEVIGQISSRFRETTLGVLRVAF
LSALVLEFLATISTALVAVTVGLRLVYAHIPFEQAFFLLLLAPEFYLPLRLLGSHFHAGL
MGVSAAGRIFAILETPLPERKLQGECHLSHDPSFQIAFEDVHYAFEDGARPALQGISFEL
HSGERVALVGPSGAGKSTAAHLLLQFMAPDRGRITVNGIPLDRIPVEEWRRHIALVPQKP
YFFYGTVAENIRLGRPGAALEEVAAAAEQAGAHQFILDLPQGYDTQVGEGGIRLSGGQAQ
RLAVARAFLKNAPLLILDEATAGLDPVNERLVLEALERLMQGRTALIIAHRLNTVHRADR
IIVLERGRVAETGRHKELIEQQGVYHRLIAAFGGAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory