SitesBLAST
Comparing WP_092482981.1 NCBI__GCF_900115975.1:WP_092482981.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
31% identity, 38% coverage: 5:307/807 of query aligns to 27:312/314 of Q16836
- GGGLMG 34:39 (≠ GAGVMG 12:17) binding NAD(+)
- A40 (= A18) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (= D35) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (≠ V57) binding CoA
- K80 (≠ G64) binding CoA
- L86 (= L70) to P: in dbSNP:rs4956145
- E122 (= E110) binding NAD(+)
- K127 (= K115) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S137) binding CoA; binding NAD(+)
- Q152 (≠ S140) to H: in dbSNP:rs1051519
- N173 (= N163) binding NAD(+)
- Y226 (≠ G217) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (≠ S250) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K300) binding NAD(+)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
30% identity, 38% coverage: 5:307/807 of query aligns to 4:289/291 of 1f0yA
- active site: S126 (= S137), H147 (= H160), E159 (= E172), N197 (= N211)
- binding acetoacetyl-coenzyme a: S50 (≠ E53), K57 (≠ N59), S126 (= S137), H147 (= H160), F149 (= F162), N150 (= N163), P151 (= P164), P153 (≠ R166), V154 (≠ Y167), N197 (= N211), P232 (= P246), M233 (≠ S248), L238 (≠ T253)
- binding nicotinamide-adenine-dinucleotide: G13 (= G14), L14 (≠ V15), M15 (= M16), D34 (= D35), Q35 (≠ I36), A96 (≠ V107), I97 (≠ V108), E99 (= E110), K104 (= K115), N124 (= N135), S126 (= S137), H147 (= H160), N150 (= N163), V242 (= V257), T246 (≠ V261), K282 (= K300)
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
29% identity, 38% coverage: 5:307/807 of query aligns to 27:312/314 of P00348
- GGGLMG 34:39 (≠ GAGVMG 12:17) binding NAD(+)
- D57 (= D35) binding NAD(+)
- E122 (= E110) binding NAD(+)
- K127 (= K115) binding NAD(+)
- S149 (= S137) binding NAD(+)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
30% identity, 38% coverage: 5:307/807 of query aligns to 4:289/293 of 1f17A
- active site: S126 (= S137), H147 (= H160), E159 (= E172), N197 (= N211)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G14), L14 (≠ V15), M15 (= M16), D34 (= D35), Q35 (≠ I36), A96 (≠ V107), I97 (≠ V108), E99 (= E110), K104 (= K115), N124 (= N135), S126 (= S137), H147 (= H160)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
30% identity, 38% coverage: 5:307/807 of query aligns to 4:289/293 of 1f12A
- active site: S126 (= S137), H147 (= H160), E159 (= E172), N197 (= N211)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ R60), S126 (= S137), H147 (= H160), F149 (= F162), N150 (= N163), M155 (= M168), N197 (= N211), L200 (≠ G214), P232 (= P246), M233 (≠ S248), L238 (≠ T253)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 38% coverage: 1:306/807 of query aligns to 1:284/286 of P9WNP7
- S122 (= S137) mutation to A: Loss of fatty acyl dehydrogenase activity.
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
28% identity, 37% coverage: 7:306/807 of query aligns to 2:277/281 of 6aa8E
- active site: S116 (= S137), H137 (= H160), E149 (= E172), N187 (= N211)
- binding nicotinamide-adenine-dinucleotide: I6 (≠ L11), G9 (= G14), T10 (≠ V15), M11 (= M16), R29 (≠ L34), D30 (= D35), I31 (= I36), A86 (≠ V107), A87 (≠ V108), E89 (= E110), K94 (= K115), N114 (= N135), S116 (= S137)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
29% identity, 37% coverage: 5:306/807 of query aligns to 1:278/282 of 4kugA
- active site: S117 (= S137), H138 (= H160), E150 (= E172), N188 (= N211)
- binding nicotinamide-adenine-dinucleotide: G10 (= G14), T11 (≠ V15), M12 (= M16), R30 (≠ L34), D31 (= D35), A87 (≠ V107), A88 (≠ V108), E90 (= E110), N115 (= N135), S117 (= S137), H138 (= H160)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
29% identity, 37% coverage: 5:306/807 of query aligns to 1:278/280 of 4kuhA
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
30% identity, 38% coverage: 4:306/807 of query aligns to 1:279/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
30% identity, 38% coverage: 4:306/807 of query aligns to 1:279/283 of 4pzdA
- active site: S118 (= S137), H139 (= H160), E151 (= E172), N189 (= N211)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), T12 (≠ V15), M13 (= M16), D32 (= D35), A88 (≠ V107), A89 (≠ V108), T90 (≠ V109), E91 (= E110), I99 (≠ L118), N116 (= N135), S118 (= S137), N142 (= N163)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
30% identity, 39% coverage: 3:313/807 of query aligns to 289:571/692 of 6iunB
- active site: S407 (= S137), H428 (= H160), E440 (= E172), N478 (= N211)
- binding nicotinamide-adenine-dinucleotide: G300 (= G14), T301 (≠ V15), M302 (= M16), E321 (≠ D35), T322 (≠ I36), Y365 (≠ M95), A377 (≠ V107), V378 (= V108), E380 (= E110), V384 (≠ I114), V388 (≠ L118), N405 (= N135), S407 (= S137)
Sites not aligning to the query:
9jheA 3-hydroxybutyryl-coa dehydrogenase with NAD (see paper)
29% identity, 37% coverage: 7:306/807 of query aligns to 2:276/289 of 9jheA
- binding nicotinamide-adenine-dinucleotide: I6 (≠ L11), G7 (= G12), G9 (= G14), T10 (≠ V15), M11 (= M16), D31 (= D35), I32 (= I36), K74 (≠ F91), A87 (≠ V107), A88 (≠ V108), F89 (≠ V109), E90 (= E110), N115 (= N135), S117 (= S137), H136 (= H160)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
29% identity, 38% coverage: 2:306/807 of query aligns to 358:637/763 of P40939
- E510 (= E172) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
9jhyB 3-hydroxybutyryl-coa dehydrogenase mutant (s117a) with acetoacetyl coa (see paper)
29% identity, 37% coverage: 7:306/807 of query aligns to 2:272/285 of 9jhyB