SitesBLAST
Comparing WP_092483750.1 NCBI__GCF_900115975.1:WP_092483750.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
41% identity, 90% coverage: 1:230/255 of query aligns to 1:233/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ C6), V7 (≠ L7), D39 (= D38), I63 (≠ V61), V101 (≠ T96), A119 (≠ G114), G120 (= G115), Q122 (≠ R117), A123 (= A118), N126 (≠ D121), M128 (≠ S123), N129 (≠ A124), T131 (≠ V126)
- binding flavin-adenine dinucleotide: Y13 (≠ T13), L182 (≠ K177)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
39% identity, 98% coverage: 1:251/255 of query aligns to 1:259/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (= C6), A7 (≠ L7), K8 (= K8), D38 (= D38), T61 (≠ S60), M62 (≠ V61), A93 (≠ L91), D94 (= D92), T95 (≠ E93), T98 (= T96), L102 (= L100), G118 (= G115), Q120 (≠ R117), G124 (≠ D121), T126 (≠ S123), A127 (= A124), V129 (= V126), T223 (≠ V216)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 98% coverage: 1:251/255 of query aligns to 1:257/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (= C6), I7 (≠ L7), K8 (= K8), D38 (= D38), M61 (≠ V61), A92 (≠ L91), D93 (= D92), T94 (≠ E93), T97 (= T96), A115 (≠ G114), G116 (= G115), A119 (= A118), G122 (≠ D121), T124 (≠ S123), A125 (= A124), V127 (= V126)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
39% identity, 90% coverage: 1:230/255 of query aligns to 4:236/255 of P38117
- A9 (≠ C6) binding AMP
- NPFC 39:42 (≠ NPYD 35:38) binding AMP
- C66 (≠ V61) binding AMP
- 123:134 (vs. 115:126, 50% identical) binding AMP
- D128 (= D120) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ E146) to M: in dbSNP:rs1130426
- R164 (= R156) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (= E157) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 175:197, 61% identical) Recognition loop
- L195 (≠ M187) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (= K192) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (= KAK 192:194) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (= KAKK 192:195) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
38% identity, 90% coverage: 1:230/255 of query aligns to 4:236/255 of Q9DCW4
- K200 (= K192) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
38% identity, 90% coverage: 1:230/255 of query aligns to 4:236/255 of Q2TBV3
- K200 (= K192) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
39% identity, 90% coverage: 1:230/255 of query aligns to 1:233/252 of 2a1uB
- binding adenosine monophosphate: A6 (≠ C6), V7 (≠ L7), K8 (= K8), C39 (≠ D38), C63 (≠ V61), V101 (≠ T96), L119 (≠ G114), G120 (= G115), Q122 (≠ R117), A123 (= A118), D126 (= D121), C128 (≠ S123), N129 (≠ A124), Q130 (= Q125), T131 (≠ V126)
- binding flavin-adenine dinucleotide: P37 (= P36), L182 (≠ K177)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
41% identity, 76% coverage: 1:195/255 of query aligns to 1:196/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ L7), K8 (= K8), Y37 (= Y37), D38 (= D38), Y40 (≠ F40), T60 (≠ S60), M61 (≠ V61), A92 (≠ L91), D93 (= D92), T94 (≠ E93), T97 (= T96), M101 (≠ L100), C116 (≠ G114), G117 (= G115), Q119 (≠ R117), A120 (= A118), G123 (≠ D121), T125 (≠ S123), A126 (= A124), V128 (= V126)
- binding nicotinamide-adenine-dinucleotide: M61 (≠ V61), G62 (= G62), P63 (= P63), S85 (vs. gap), D86 (= D85), G91 (≠ E90)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
41% identity, 76% coverage: 1:195/255 of query aligns to 1:196/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ L7), K8 (= K8), Y37 (= Y37), D38 (= D38), Y40 (≠ F40), M61 (≠ V61), A92 (≠ L91), D93 (= D92), T94 (≠ E93), T97 (= T96), C116 (≠ G114), G117 (= G115), Q119 (≠ R117), A120 (= A118), G123 (≠ D121), T125 (≠ S123), A126 (= A124), V128 (= V126)
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
35% identity, 98% coverage: 1:250/255 of query aligns to 1:259/261 of 1o96A
- binding adenosine monophosphate: A6 (≠ C6), V7 (≠ L7), K8 (= K8), D38 (= D38), V63 (vs. gap), V99 (≠ T96), A117 (≠ G114), G118 (= G115), Q120 (≠ R117), S121 (≠ A118), Y126 (≠ S123), A127 (= A124), T129 (≠ V126)
- binding flavin-adenine dinucleotide: Q120 (≠ R117), Q182 (= Q176)
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
34% identity, 98% coverage: 1:250/255 of query aligns to 1:260/264 of P53570
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
35% identity, 95% coverage: 2:243/255 of query aligns to 3:258/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (= C6), I8 (≠ L7), K9 (= K8), D39 (= D38), M62 (≠ V61), A93 (≠ L91), D94 (= D92), T95 (≠ E93), T98 (= T96), L102 (= L100), T121 (≠ G114), G122 (= G115), A125 (= A118), G128 (≠ D121), T130 (≠ S123), G131 (≠ A124), V133 (= V126)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
35% identity, 76% coverage: 3:197/255 of query aligns to 4:201/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (= C6), K9 (= K8), D39 (= D38), M62 (≠ V61), D94 (= D92), V95 (≠ E93), L102 (= L100), G118 (= G115), Q120 (≠ R117), T121 (≠ A118), T126 (≠ S123), A127 (= A124), V129 (= V126)
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
39% identity, 84% coverage: 37:251/255 of query aligns to 38:248/251 of 5ow0B
- binding adenosine monophosphate: D39 (= D38), Y62 (≠ V61), I95 (≠ T96), A114 (≠ G114), G115 (= G115), Q117 (≠ R117), S118 (≠ A118), R123 (≠ S123), G124 (≠ A124), V125 (≠ Q125), V126 (= V126), P127 (≠ A127)
- binding flavin-adenine dinucleotide: Y38 (= Y37)
Sites not aligning to the query:
Query Sequence
>WP_092483750.1 NCBI__GCF_900115975.1:WP_092483750.1
MNIVVCLKQTFDTEAKISLDAGGKIDCKGVSLIMNPYDEFAVEEGLRLKESAGGEVTVIS
VGPGSAQDALRQALAMGADKAVLVDTGDAELDEYATATVLAKAIEDVGYDIILGGFRAID
DGSAQVAGRVAEILNLPVVNVVTELEVADGRAKATREIEGGSEVVEVPLPAVITAQKGLN
EPRYPSMKGIMKAKKKPMDKKTLADLGLDGQVAAKVKALSYTLPEPRKAGKVIPGEAAEA
AAELAKLLREEAKVI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory