SitesBLAST
Comparing WP_092484009.1 NCBI__GCF_900115975.1:WP_092484009.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3d31A Modbc from methanosarcina acetivorans (see paper)
44% identity, 100% coverage: 1:344/344 of query aligns to 1:347/348 of 3d31A
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
38% identity, 95% coverage: 18:343/344 of query aligns to 24:365/375 of 2d62A
1g291 Malk (see paper)
40% identity, 79% coverage: 2:273/344 of query aligns to 4:294/372 of 1g291
- binding magnesium ion: D69 (≠ T66), E71 (vs. gap), K72 (vs. gap), K79 (≠ E70), D80 (≠ Q71), E292 (= E271), D293 (= D272)
- binding pyrophosphate 2-: S38 (≠ T35), G39 (= G36), C40 (≠ T37), G41 (= G38), K42 (= K39), T43 (= T40), T44 (≠ V41)
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
42% identity, 70% coverage: 2:243/344 of query aligns to 7:243/353 of 1vciA
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 86% coverage: 10:306/344 of query aligns to 14:315/353 of 1oxvD
Sites not aligning to the query:
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 86% coverage: 10:306/344 of query aligns to 14:315/353 of 1oxvA
Sites not aligning to the query:
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 86% coverage: 10:306/344 of query aligns to 14:315/353 of 1oxuA
Sites not aligning to the query:
Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
39% identity, 86% coverage: 10:306/344 of query aligns to 14:315/353 of Q97UY8
- S142 (= S132) mutation to A: Decrease in ATPase activity. Can form dimers.
- G144 (= G134) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
- E166 (= E156) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 67% coverage: 2:232/344 of query aligns to 3:234/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
41% identity, 67% coverage: 2:232/344 of query aligns to 3:234/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ L11), S37 (≠ T35), G38 (= G36), C39 (≠ T37), G40 (= G38), K41 (= K39), S42 (≠ T40), T43 (≠ V41), Q81 (= Q79), R128 (= R126), A132 (≠ T130), S134 (= S132), G136 (= G134), Q137 (≠ E135), E158 (= E156), H191 (= H189)
- binding magnesium ion: S42 (≠ T40), Q81 (= Q79)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
41% identity, 67% coverage: 2:232/344 of query aligns to 3:234/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ L11), G38 (= G36), C39 (≠ T37), G40 (= G38), K41 (= K39), S42 (≠ T40), T43 (≠ V41), R128 (= R126), S134 (= S132), Q137 (≠ E135)
- binding beryllium trifluoride ion: S37 (≠ T35), G38 (= G36), K41 (= K39), Q81 (= Q79), S134 (= S132), G136 (= G134), H191 (= H189)
- binding magnesium ion: S42 (≠ T40), Q81 (= Q79)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
41% identity, 67% coverage: 2:232/344 of query aligns to 3:234/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ L11), V17 (≠ L16), G38 (= G36), C39 (≠ T37), G40 (= G38), K41 (= K39), S42 (≠ T40), T43 (≠ V41), R128 (= R126), A132 (≠ T130), S134 (= S132), Q137 (≠ E135)
- binding tetrafluoroaluminate ion: S37 (≠ T35), G38 (= G36), K41 (= K39), Q81 (= Q79), S134 (= S132), G135 (= G133), G136 (= G134), E158 (= E156), H191 (= H189)
- binding magnesium ion: S42 (≠ T40), Q81 (= Q79)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
41% identity, 67% coverage: 2:232/344 of query aligns to 3:234/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ L11), V17 (≠ L16), G38 (= G36), C39 (≠ T37), G40 (= G38), K41 (= K39), S42 (≠ T40), T43 (≠ V41), R128 (= R126), A132 (≠ T130), S134 (= S132), Q137 (≠ E135)
- binding magnesium ion: S42 (≠ T40), Q81 (= Q79)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
41% identity, 67% coverage: 2:232/344 of query aligns to 4:235/371 of P68187
- A85 (= A82) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P103) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ L111) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L114) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A116) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ G121) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G134) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D155) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ D225) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
41% identity, 67% coverage: 2:232/344 of query aligns to 1:232/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ L11), S35 (≠ T35), G36 (= G36), C37 (≠ T37), G38 (= G38), K39 (= K39), S40 (≠ T40), T41 (≠ V41), R126 (= R126), A130 (≠ T130), S132 (= S132), G134 (= G134), Q135 (≠ E135)
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 78% coverage: 4:273/344 of query aligns to 6:287/393 of P9WQI3
- H193 (= H189) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
8hplC Lpqy-sugabc in state 1 (see paper)
36% identity, 78% coverage: 4:273/344 of query aligns to 5:284/384 of 8hplC