SitesBLAST
Comparing WP_092487896.1 NCBI__GCF_900115975.1:WP_092487896.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
49% identity, 100% coverage: 1:260/260 of query aligns to 1:259/259 of 5zaiC
- active site: A65 (= A66), F70 (≠ M71), S82 (≠ A83), R86 (≠ Q87), G110 (= G111), E113 (= E114), P132 (= P133), E133 (= E134), I138 (≠ L139), P140 (≠ A141), G141 (= G142), A226 (≠ V227), F236 (= F237)
- binding coenzyme a: K24 (≠ V24), L25 (= L25), A63 (= A64), G64 (= G65), A65 (= A66), D66 (= D67), I67 (= I68), P132 (= P133), R166 (≠ M167), F248 (= F249), K251 (= K252)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
42% identity, 99% coverage: 1:257/260 of query aligns to 1:254/255 of 3q0jC
- active site: A65 (= A66), M70 (= M71), T80 (≠ E78), F84 (= F82), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ L139), P138 (≠ A141), G139 (= G142), L224 (≠ V227), F234 (= F237)
- binding acetoacetyl-coenzyme a: Q23 (≠ K23), A24 (≠ V24), L25 (= L25), A27 (= A27), A63 (= A64), G64 (= G65), A65 (= A66), D66 (= D67), I67 (= I68), K68 (≠ A69), M70 (= M71), F84 (= F82), G107 (= G110), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), P138 (≠ A141), G139 (= G142), M140 (≠ F143)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
42% identity, 99% coverage: 1:257/260 of query aligns to 1:254/255 of 3q0gC
- active site: A65 (= A66), M70 (= M71), T80 (≠ E78), F84 (= F82), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ L139), P138 (≠ A141), G139 (= G142), L224 (≠ V227), F234 (= F237)
- binding coenzyme a: L25 (= L25), A63 (= A64), I67 (= I68), K68 (≠ A69), Y104 (≠ F107), P130 (= P133), E131 (= E134), L134 (= L137)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
41% identity, 99% coverage: 3:260/260 of query aligns to 2:256/256 of 3h81A
- active site: A64 (= A66), M69 (= M71), T79 (≠ E78), F83 (= F82), G107 (= G111), E110 (= E114), P129 (= P133), E130 (= E134), V135 (≠ L139), P137 (≠ A141), G138 (= G142), L223 (≠ V227), F233 (= F237)
- binding calcium ion: F233 (= F237), Q238 (≠ R242)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
41% identity, 98% coverage: 3:257/260 of query aligns to 2:249/250 of 3q0gD
- active site: A64 (= A66), M69 (≠ L77), T75 (≠ A83), F79 (≠ Q87), G103 (= G111), E106 (= E114), P125 (= P133), E126 (= E134), V131 (≠ L139), P133 (≠ A141), G134 (= G142), L219 (≠ V227), F229 (= F237)
- binding Butyryl Coenzyme A: F225 (= F233), F241 (= F249)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
44% identity, 96% coverage: 10:258/260 of query aligns to 12:252/254 of 2dubA
- active site: A67 (= A66), M72 (= M71), S82 (= S91), G105 (= G111), E108 (= E114), P127 (= P133), E128 (= E134), T133 (≠ L139), P135 (≠ A141), G136 (= G142), K221 (≠ V227), F231 (= F237)
- binding octanoyl-coenzyme a: K25 (= K23), A26 (≠ V24), L27 (= L25), A29 (= A27), A65 (= A64), A67 (= A66), D68 (= D67), I69 (= I68), K70 (≠ A69), G105 (= G111), E108 (= E114), P127 (= P133), E128 (= E134), G136 (= G142), A137 (≠ F143)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
44% identity, 96% coverage: 10:258/260 of query aligns to 13:256/258 of 1mj3A
- active site: A68 (= A66), M73 (= M71), S83 (≠ N81), L85 (≠ Q87), G109 (= G111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ L139), P139 (≠ A141), G140 (= G142), K225 (≠ V227), F235 (= F237)
- binding hexanoyl-coenzyme a: K26 (= K23), A27 (≠ V24), L28 (= L25), A30 (= A27), A66 (= A64), G67 (= G65), A68 (= A66), D69 (= D67), I70 (= I68), G109 (= G111), P131 (= P133), E132 (= E134), L135 (= L137), G140 (= G142)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
44% identity, 96% coverage: 10:258/260 of query aligns to 13:258/260 of 1dubA
- active site: A68 (= A66), M73 (= M71), S83 (≠ N81), L87 (= L85), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ L139), P141 (≠ A141), G142 (= G142), K227 (≠ V227), F237 (= F237)
- binding acetoacetyl-coenzyme a: K26 (= K23), A27 (≠ V24), L28 (= L25), A30 (= A27), A66 (= A64), A68 (= A66), D69 (= D67), I70 (= I68), Y107 (≠ F107), G110 (= G110), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), L137 (= L137), G142 (= G142), F233 (= F233), F249 (= F249)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
44% identity, 96% coverage: 10:258/260 of query aligns to 11:256/258 of 1ey3A
- active site: A66 (= A66), M71 (= M71), S81 (≠ N81), L85 (= L85), G109 (= G111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ L139), P139 (≠ A141), G140 (= G142), K225 (≠ V227), F235 (= F237)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K23), L26 (= L25), A28 (= A27), A64 (= A64), G65 (= G65), A66 (= A66), D67 (= D67), I68 (= I68), L85 (= L85), W88 (≠ F90), G109 (= G111), P131 (= P133), L135 (= L137), G140 (= G142)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
44% identity, 96% coverage: 10:258/260 of query aligns to 43:288/290 of P14604
- E144 (= E114) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E134) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
43% identity, 95% coverage: 14:260/260 of query aligns to 17:260/260 of 2hw5C
- active site: A68 (= A66), M73 (= M71), S83 (≠ N81), L87 (= L85), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ L139), P141 (≠ A141), G142 (= G142), K227 (≠ V227), F237 (= F237)
- binding crotonyl coenzyme a: K26 (= K23), A27 (≠ V24), L28 (= L25), A30 (= A27), K62 (= K60), I70 (= I68), F109 (≠ L109)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
40% identity, 95% coverage: 14:260/260 of query aligns to 15:261/261 of 5jbxB
- active site: A67 (= A66), R72 (≠ M71), L84 (≠ A83), R88 (≠ Q87), G112 (= G111), E115 (= E114), T134 (≠ P133), E135 (= E134), I140 (≠ L139), P142 (≠ A141), G143 (= G142), A228 (≠ V227), L238 (≠ F237)
- binding coenzyme a: S24 (≠ K23), R25 (≠ V24), R26 (≠ L25), A28 (= A27), A65 (= A64), D68 (= D67), L69 (≠ I68), K70 (≠ A69), L110 (= L109), G111 (= G110), T134 (≠ P133), E135 (= E134), L138 (= L137), R168 (≠ M167)
Q13825 Methylglutaconyl-CoA hydratase, mitochondrial; 3-MG-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydratase; Itaconyl-CoA hydratase; EC 4.2.1.18; EC 4.2.1.56 from Homo sapiens (Human) (see 4 papers)
39% identity, 97% coverage: 7:259/260 of query aligns to 82:338/339 of Q13825
- K105 (≠ A30) mutation to N: Abolishes RNA-binding; when associated with E-109 and Q-113.
- 105:119 (vs. 30:44, 27% identical) RNA-binding
- K109 (≠ Q34) mutation to E: Abolishes RNA-binding; when associated with N-105 and Q-113.
- K113 (≠ A38) mutation to Q: Abolishes RNA-binding; when associated with N-105 and E-109.
- A240 (= A165) to V: in MGCA1; decreased methylglutaconyl-CoA hydratase activity; dbSNP:rs769894315
Sites not aligning to the query:
- 1:67 modified: transit peptide, Mitochondrion
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 98% coverage: 6:260/260 of query aligns to 5:257/257 of 6slbAAA
- active site: Q64 (≠ A66), F69 (≠ M71), L80 (≠ A83), N84 (≠ Q87), A108 (≠ G111), S111 (≠ E114), A130 (≠ P133), F131 (≠ E134), L136 (= L139), P138 (≠ A141), D139 (≠ G142), A224 (≠ V227), G234 (≠ F237)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K60), A62 (= A64), Q64 (≠ A66), D65 (= D67), L66 (≠ I68), Y76 (≠ A79), A108 (≠ G111), F131 (≠ E134), D139 (≠ G142)
3h02A 2.15 angstrom resolution crystal structure of naphthoate synthase from salmonella typhimurium.
37% identity, 99% coverage: 2:258/260 of query aligns to 17:260/266 of 3h02A
- active site: G82 (≠ A66), H86 (≠ F70), L90 (= L74), G114 (= G111), V117 (≠ E114), G137 (≠ E134), S142 (≠ L139), D144 (≠ A141), G145 (= G142), A231 (≠ F226), Y239 (≠ F237)
- binding bicarbonate ion: G113 (= G110), Q135 (= Q132), G137 (≠ E134), W165 (≠ L162)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 98% coverage: 6:260/260 of query aligns to 2:245/245 of 6slaAAA