SitesBLAST
Comparing WP_092680945.1 NCBI__GCF_900110435.1:WP_092680945.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
30% identity, 46% coverage: 4:191/413 of query aligns to 49:228/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ I28), G75 (= G30), S76 (≠ H31), G77 (= G32), T78 (= T33), G79 (≠ K34), L80 (≠ R35), A83 (≠ Q39), C84 (vs. gap), P137 (= P91), G138 (≠ P101), E139 (= E102), A142 (≠ G105), T143 (= T106), G146 (= G109), N147 (≠ M110), S149 (≠ A112), T150 (≠ A113), A152 (≠ L115), G153 (≠ A116), E203 (≠ W166), G204 (= G167), I209 (≠ M172)
Sites not aligning to the query:
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
30% identity, 42% coverage: 10:182/413 of query aligns to 50:214/459 of P9WIT1
Sites not aligning to the query:
- 354 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
31% identity, 41% coverage: 20:188/413 of query aligns to 59:222/465 of 3pm9A
- active site: A149 (≠ L115), L159 (≠ G125)
- binding flavin-adenine dinucleotide: P69 (≠ G30), Q70 (≠ H31), G71 (= G32), G72 (≠ T33), N73 (≠ K34), T74 (≠ R35), G75 (≠ P36), L76 (≠ V37), G79 (= G38), Q80 (= Q39), L91 (= L49), L133 (≠ G99), G134 (≠ T100), A135 (≠ P101), C139 (≠ G105), T140 (= T106), G142 (= G108), G143 (= G109), S146 (≠ A112), T147 (≠ A113), A149 (≠ L115), G150 (≠ A116), E200 (≠ W166), G201 (= G167), I205 (≠ V171), I206 (≠ M172)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
22% identity, 69% coverage: 4:286/413 of query aligns to 104:380/521 of Q8N465
- S109 (≠ A9) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ E27) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (≠ H31) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V46) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L52) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ A71) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ A88) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A112) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ G138) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G140) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ L281) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 386 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; R→A: Loss of catalytic activity.
- 390 binding (R)-2-hydroxyglutarate; binding (R)-malate; T→A: Significantly reduced catalytic activity.
- 399 V → M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- 401 binding (R)-2-hydroxyglutarate; binding (R)-malate; K→A: Loss of catalytic activity.
- 419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- 426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- 434 binding Zn(2+); H→A: Loss of catalytic activity.
- 436 G → V: slight reduction in catalytic activity
- 439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- 441 binding Zn(2+); H→A: Loss of catalytic activity.
- 443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
- 444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- 446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- 475 binding Zn(2+); E→A: Loss of catalytic activity.
- 476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
- 477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
23% identity, 69% coverage: 4:286/413 of query aligns to 52:328/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (≠ I28), G78 (= G30), G79 (≠ H31), N80 (≠ G32), T81 (= T33), G82 (≠ K34), M83 (≠ R35), G86 (= G38), S87 (≠ Q39), L140 (= L98), A142 (≠ T100), C146 (≠ G105), H147 (≠ T106), G150 (= G109), N151 (≠ M110), A153 (= A112), T154 (≠ A113), G208 (= G167), I212 (≠ V171), I213 (≠ M172)
Sites not aligning to the query:
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
23% identity, 69% coverage: 4:286/413 of query aligns to 51:327/466 of 6lpxA
- binding flavin-adenine dinucleotide: P75 (≠ I28), Q76 (≠ I29), G77 (= G30), G78 (≠ H31), N79 (≠ G32), T80 (= T33), G81 (≠ K34), M82 (≠ R35), G85 (= G38), S86 (≠ Q39), L139 (= L98), G140 (= G99), A141 (≠ T100), C145 (≠ G105), G149 (= G109), N150 (≠ M110), A152 (= A112), T153 (≠ A113), G157 (= G117), G207 (= G167), I212 (≠ M172)
Sites not aligning to the query:
- binding 2-oxoglutaric acid: 333, 337, 348, 379, 381, 388, 423
- binding flavin-adenine dinucleotide: 39, 422, 459
- binding zinc ion: 381, 388, 422
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
23% identity, 69% coverage: 4:286/413 of query aligns to 51:327/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (≠ I28), G77 (= G30), G78 (≠ H31), N79 (≠ G32), T80 (= T33), G81 (≠ K34), G85 (= G38), S86 (≠ Q39), L139 (= L98), G140 (= G99), A141 (≠ T100), C145 (≠ G105), H146 (≠ T106), G148 (= G108), G149 (= G109), N150 (≠ M110), A152 (= A112), T153 (≠ A113), A155 (≠ L115), E206 (≠ W166), G207 (= G167), I211 (≠ V171), I212 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 39, 422, 459
- binding (2s)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 423
- binding zinc ion: 381, 388, 422
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
23% identity, 69% coverage: 4:286/413 of query aligns to 51:327/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (≠ I28), G77 (= G30), G78 (≠ H31), N79 (≠ G32), T80 (= T33), G81 (≠ K34), G85 (= G38), S86 (≠ Q39), L139 (= L98), G140 (= G99), A141 (≠ T100), C145 (≠ G105), H146 (≠ T106), G149 (= G109), N150 (≠ M110), A152 (= A112), T153 (≠ A113), A155 (≠ L115), G157 (= G117), E206 (≠ W166), G207 (= G167), I211 (≠ V171), I212 (≠ M172)
- binding d-malate: M82 (≠ R35)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 39, 422, 459
- binding d-malate: 333, 337, 348, 379, 381, 388, 422, 423
- binding zinc ion: 381, 388, 422
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
23% identity, 69% coverage: 4:286/413 of query aligns to 51:327/466 of 6lppA
- binding flavin-adenine dinucleotide: P75 (≠ I28), G77 (= G30), G78 (≠ H31), N79 (≠ G32), T80 (= T33), G81 (≠ K34), M82 (≠ R35), G85 (= G38), S86 (≠ Q39), L139 (= L98), G140 (= G99), A141 (≠ T100), C145 (≠ G105), G149 (= G109), N150 (≠ M110), A152 (= A112), T153 (≠ A113), A155 (≠ L115), G157 (= G117), G207 (= G167), I212 (≠ M172)
Sites not aligning to the query:
- binding (2R)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 390, 423
- binding flavin-adenine dinucleotide: 39, 422, 423
- binding zinc ion: 381, 388, 422
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/454 of 8jdvA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding 2-Ketohexanoic acid: 75, 317, 321, 368, 375, 413
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 321, 322, 412, 413, 449
- binding manganese (ii) ion: 368, 375, 412
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdrA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 324, 370, 414, 451
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: 319, 323, 415
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdqA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding (2R)-2-hydroxy-4-methylpentanoic acid: 319, 323, 370, 415
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 370, 414, 451
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdoA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding (2R)-2-hydroxyhexanoic acid: 319, 323, 415
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 324, 370, 414, 451
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdnA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 370, 414, 451
- binding (2R)-2-oxidanylpentanoic acid: 319, 323, 415
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdgA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 370, 414, 451
- binding (2R)-2-oxidanylbutanoic acid: 319, 415
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdbA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 324, 370, 414, 451
- binding (2R)-2-oxidanyloctanoic acid: 75, 319, 323, 415
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/455 of 8jdxA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 90, 322, 413, 414, 450
- binding 3-methyl-2-oxobutanoic acid: 318, 369, 376, 414
- binding manganese (ii) ion: 369, 376, 413
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/456 of 8jdsA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 90, 323, 414, 415, 451
- binding manganese (ii) ion: 370, 377, 414
- binding pyruvic acid: 319, 370, 377, 415
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/455 of 8jdtA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding 2-ketobutyric acid: 318, 369, 376, 414
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 322, 413, 414, 450
- binding manganese (ii) ion: 369, 376, 413
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
32% identity, 21% coverage: 101:185/413 of query aligns to 132:219/454 of 8jdzA
- binding flavin-adenine dinucleotide: P132 (= P101), G133 (≠ E102), A134 (≠ T103), G140 (= G109), M141 (= M110), A143 (= A112), T144 (≠ A113), A146 (≠ L115), S147 (≠ A116), E200 (≠ W166), G201 (= G167), I206 (≠ M172)
Sites not aligning to the query:
- binding (3S)-3-methyl-2-oxopentanoic acid: 318, 322, 369, 376, 413
- binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 90, 322, 412, 413, 449
- binding manganese (ii) ion: 369, 376, 412
Query Sequence
>WP_092680945.1 NCBI__GCF_900110435.1:WP_092680945.1
MDTLKVRDAQEVEDAVRAAIANEQPLEIIGHGTKRPVGQPMATNAVLELSALNAVTAYEP
NELIITVQAGAPVADVLSLIDSRNQQFAFEPINTSLLLGTPETEGTIGGMIAAGLAGPRR
IKSGGARDHLLGAHAVSGFGESFKAGGKVVKNVTGYDLCKLLAGSWGTLAVMTEVTLKVT
PRPESERTLVLRGLDDVTANRAMTQALGSPYDVSGAAHLPGSALRTTGGALADIASLDQA
LTLLRLEGITSSASHRAQSLREALKSFGTADLIEDEASADLWRAVRDVEPFAASGPRALW
PVWRIVCPPASGGAFGLALQRESGGEVIYDWGGGLIWAALPPAADGHAKALRQRVDKIGG
HASLIRASDEVRASVDVFQPQPSGLAALGLRVKHSFDPRNILNRGRLSRGPAV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory