SitesBLAST
Comparing WP_092992765.1 NCBI__GCF_900102855.1:WP_092992765.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4itbA Structure of bacterial enzyme in complex with cofactor and substrate (see paper)
50% identity, 100% coverage: 2:454/454 of query aligns to 1:453/453 of 4itbA
- active site: N130 (= N131), K153 (= K154), E227 (= E228), C261 (= C262), E358 (= E359), E435 (= E436)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V126 (= V127), M127 (= M128), P128 (= P129), W129 (= W130), N130 (= N131), K153 (= K154), A155 (= A156), S156 (= S157), A186 (= A187), V189 (= V190), G205 (= G206), S206 (= S207), A209 (= A210), S212 (≠ K213), L228 (= L229), C261 (= C262), E358 (= E359), F360 (= F361)
- binding 4-oxobutanoic acid: E227 (= E228), C261 (= C262), S418 (= S419)
3vz3A Structural insights into substrate and cofactor selection by sp2771 (see paper)
49% identity, 100% coverage: 2:454/454 of query aligns to 1:453/453 of 3vz3A
- active site: N130 (= N131), K153 (= K154), E227 (= E228), A261 (≠ C262), E358 (= E359), E435 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V126 (= V127), M127 (= M128), W129 (= W130), N130 (= N131), Q135 (= Q136), R138 (= R139), K153 (= K154), A155 (= A156), S156 (= S157), A186 (= A187), V189 (= V190), T204 (= T205), G205 (= G206), S206 (= S207), A209 (= A210), E227 (= E228), L228 (= L229), G229 (= G230), A261 (≠ C262), F360 (= F361)
- binding 4-oxobutanoic acid: F131 (= F132), W134 (= W135), S260 (= S261), A261 (≠ C262), I262 (= I263), S418 (= S419)
3efvA Crystal structure of a putative succinate-semialdehyde dehydrogenase from salmonella typhimurium lt2 with bound NAD (see paper)
50% identity, 99% coverage: 5:452/454 of query aligns to 8:455/459 of 3efvA
- active site: N134 (= N131), E231 (= E228), C265 (= C262), E439 (= E436)
- binding nicotinamide-adenine-dinucleotide: I130 (≠ V127), M131 (= M128), P132 (= P129), W133 (= W130), N134 (= N131), Q139 (= Q136), R142 (= R139), K157 (= K154), A159 (= A156), N190 (≠ A187), V193 (= V190), T208 (= T205), G209 (= G206), S210 (= S207), A213 (= A210), E231 (= E228), L232 (= L229), G233 (= G230), C265 (= C262), E362 (= E359), F364 (= F361), F428 (= F425)
4ywuA Structural insight into the substrate inhibition mechanism of NADP+- dependent succinic semialdehyde dehydrogenase from streptococcus pyogenes (see paper)
39% identity, 100% coverage: 2:453/454 of query aligns to 1:453/455 of 4ywuA
- active site: N131 (= N131), K154 (= K154), E228 (= E228), C262 (= C262), E359 (= E359), E436 (= E436)
- binding 4-oxobutanoic acid: N131 (= N131), Q136 (= Q136), R139 (= R139), E228 (= E228), V261 (≠ S261), C262 (= C262), F425 (= F425)
4ohtA Crystal structure of succinic semialdehyde dehydrogenase from streptococcus pyogenes in complex with NADP+ as the cofactor (see paper)
39% identity, 100% coverage: 2:453/454 of query aligns to 1:453/455 of 4ohtA
- active site: N131 (= N131), K154 (= K154), E228 (= E228), C262 (= C262), E359 (= E359), E436 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V127 (= V127), E128 (≠ M128), P129 (= P129), W130 (= W130), K154 (= K154), H155 (= H155), A156 (= A156), S157 (= S157), Y187 (≠ A187), S207 (= S207), I214 (≠ V214)
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
35% identity, 100% coverage: 2:453/454 of query aligns to 27:480/482 of P25526
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
35% identity, 100% coverage: 2:453/454 of query aligns to 26:479/481 of 3jz4A
- active site: N156 (= N131), K179 (= K154), E254 (= E228), C288 (= C262), E385 (= E359), E462 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P129), W155 (= W130), K179 (= K154), A181 (= A156), S182 (= S157), A212 (= A187), G216 (vs. gap), G232 (= G206), S233 (= S207), I236 (≠ A210), C288 (= C262), K338 (≠ D312), E385 (= E359), F387 (= F361)
8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
31% identity, 98% coverage: 7:453/454 of query aligns to 49:497/505 of 8of1A
- binding nicotinamide-adenine-dinucleotide: I170 (≠ V127), A171 (≠ M128), P172 (= P129), W173 (= W130), K197 (= K154), A230 (= A187), F248 (≠ L204), G250 (= G206), S251 (= S207), V254 (≠ A210), M257 (≠ K213), L273 (= L229), C306 (= C262), K356 (≠ D312), E403 (= E359), F405 (= F361)
4go4A Crystal structure of pnpe in complex with nicotinamide adenine dinucleotide
31% identity, 99% coverage: 6:454/454 of query aligns to 23:476/487 of 4go4A
- active site: N149 (= N131), K172 (= K154), E247 (= E228), C281 (= C262), E381 (= E359), E458 (= E436)
- binding nicotinamide-adenine-dinucleotide: I145 (≠ V127), V146 (≠ M128), W148 (= W130), N149 (= N131), F154 (≠ Q136), K172 (= K154), G205 (= G186), G209 (≠ V190), Q210 (≠ E191), F223 (≠ L204), T224 (= T205), G225 (= G206), S226 (= S207), T229 (≠ A210), E247 (= E228), G249 (= G230), C281 (= C262), E381 (= E359), F383 (= F361)
8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
35% identity, 91% coverage: 39:451/454 of query aligns to 63:477/482 of 8c54A
- binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (≠ V127), T153 (≠ M128), P154 (= P129), K179 (= K154), A212 (≠ S188), K213 (≠ Q189), F230 (≠ L204), T231 (= T205), G232 (= G206), S233 (= S207), V236 (≠ A210), W239 (≠ K213), G256 (= G230)
P25553 Lactaldehyde dehydrogenase; Aldehyde dehydrogenase A; Glycolaldehyde dehydrogenase; EC 1.2.1.22; EC 1.2.1.21 from Escherichia coli (strain K12) (see 5 papers)
30% identity, 99% coverage: 6:454/454 of query aligns to 27:478/479 of P25553
- L150 (≠ M128) binding NAD(+)
- R161 (= R139) binding (S)-lactate
- KPSE 176:179 (≠ KHAS 154:157) binding NAD(+)
- F180 (≠ N158) mutation to T: Can bind and use NADP(+) as coenzyme. 16-fold increase in catalytic efficiency with NAD(+) as coenzyme.
- Q214 (vs. gap) binding NAD(+)
- S230 (= S207) binding NAD(+)
- E251 (= E228) binding (S)-lactate
- N286 (≠ I263) binding (S)-lactate; mutation to E: 4-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to H: 15-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to T: 6-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.
- R336 (≠ D312) binding NAD(+)
- E443 (≠ S419) binding (S)-lactate
- H449 (≠ F425) binding (S)-lactate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2impA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the ternary complex with lactate (occupancy 0.5) and nadh. Crystals soaked with (l)-lactate. (see paper)
30% identity, 99% coverage: 6:454/454 of query aligns to 25:476/477 of 2impA
- active site: N151 (= N131), K174 (= K154), E249 (= E228), C283 (= C262), E381 (= E359), A458 (≠ E436)
- binding 1,4-dihydronicotinamide adenine dinucleotide: I147 (≠ V127), L148 (≠ M128), P149 (= P129), W150 (= W130), K174 (= K154), E177 (≠ S157), F178 (≠ N158), G207 (≠ S188), G211 (= G192), Q212 (vs. gap), S228 (= S207), A231 (= A210), K234 (= K213), R334 (≠ D312)
2iluA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the binary complex with NADPH (see paper)
30% identity, 99% coverage: 6:454/454 of query aligns to 25:476/477 of 2iluA
- active site: N151 (= N131), K174 (= K154), E249 (= E228), C283 (= C262), E381 (= E359), A458 (≠ E436)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I147 (≠ V127), L148 (≠ M128), P149 (= P129), W150 (= W130), K174 (= K154), S176 (≠ A156), E177 (≠ S157), R206 (≠ A187), G207 (≠ S188), G211 (= G192), Q212 (vs. gap), S228 (= S207), A231 (= A210), K234 (= K213), I235 (≠ V214), N328 (≠ R306), R334 (≠ D312), F383 (= F361)
2opxA Crystal structure of lactaldehyde dehydrogenase from escherichia coli
30% identity, 99% coverage: 6:454/454 of query aligns to 25:476/477 of 2opxA
- active site: N151 (= N131), K174 (= K154), E249 (= E228), C283 (= C262), E381 (= E359), A458 (≠ E436)
- binding (3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid: F105 (≠ K86), F152 (= F132), N284 (≠ I263), F312 (≠ P291), G313 (= G292), R318 (≠ E297), D320 (vs. gap), I321 (≠ A299), A322 (≠ T300), Y362 (≠ F340), F440 (≠ K418), F440 (≠ K418), E441 (≠ S419)
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
30% identity, 99% coverage: 3:453/454 of query aligns to 23:480/489 of 4cazA
- active site: N152 (= N131), K175 (= K154), E251 (= E228), C285 (= C262), E386 (= E359), E463 (= E436)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (≠ V127), G149 (≠ M128), W151 (= W130), N152 (= N131), K175 (= K154), E178 (≠ S157), G208 (≠ A187), G212 (vs. gap), F226 (≠ L204), T227 (= T205), G228 (= G206), G229 (≠ S207), T232 (≠ A210), V236 (= V214), E251 (= E228), L252 (= L229), C285 (= C262), E386 (= E359), F388 (= F361)
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
30% identity, 99% coverage: 3:453/454 of query aligns to 23:480/489 of 2woxA
- active site: N152 (= N131), K175 (= K154), E251 (= E228), C285 (= C262), E386 (= E359), E463 (= E436)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (≠ V127), G149 (≠ M128), W151 (= W130), N152 (= N131), K175 (= K154), S177 (≠ A156), E178 (≠ S157), G208 (≠ A187), G212 (vs. gap), F226 (≠ L204), T227 (= T205), G228 (= G206), G229 (≠ S207), T232 (≠ A210), V236 (= V214), E251 (= E228), L252 (= L229), C285 (= C262), E386 (= E359), F388 (= F361)
2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
30% identity, 99% coverage: 3:453/454 of query aligns to 23:480/489 of 2wmeA
- active site: N152 (= N131), K175 (= K154), E251 (= E228), C285 (= C262), E386 (= E359), E463 (= E436)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (≠ M128), W151 (= W130), K175 (= K154), S177 (≠ A156), E178 (≠ S157), G208 (≠ A187), G212 (vs. gap), F226 (≠ L204), G228 (= G206), G229 (≠ S207), T232 (≠ A210), V236 (= V214)
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
30% identity, 99% coverage: 3:453/454 of query aligns to 24:481/490 of Q9HTJ1
- GAWN 150:153 (≠ MPWN 128:131) binding NADPH
- K162 (≠ F140) active site, Charge relay system
- KPSE 176:179 (≠ KHAS 154:157) binding NADPH
- G209 (≠ A187) binding NADPH
- GTST 230:233 (≠ SDSA 207:210) binding NADPH
- E252 (= E228) active site, Proton acceptor
- C286 (= C262) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (= E359) binding NADPH
- E464 (= E436) active site, Charge relay system
4o6rA Crystal structure of a putative aldehyde dehydrogenase from burkholderia cenocepacia
30% identity, 99% coverage: 6:453/454 of query aligns to 24:477/489 of 4o6rA
- active site: N150 (= N131), K173 (= K154), E248 (= E228), C282 (= C262), E383 (= E359), E460 (= E436)
- binding adenosine monophosphate: I146 (≠ V127), V147 (≠ M128), K173 (= K154), G206 (≠ S188), G210 (= G192), Q211 (vs. gap), F224 (≠ L204), G226 (= G206), S227 (= S207), T230 (≠ A210), R233 (≠ K213)
4jz6A Crystal structure of a salicylaldehyde dehydrogenase from pseudomonas putida g7 complexed with salicylaldehyde (see paper)
35% identity, 77% coverage: 105:453/454 of query aligns to 122:475/484 of 4jz6A
- active site: N150 (= N131), K173 (= K154), E251 (= E228), C285 (= C262), E380 (= E359), F458 (≠ E436)
- binding salicylaldehyde: G151 (≠ F132), V154 (≠ W135), R247 (≠ K224), C248 (≠ S225), I284 (≠ S261), C285 (= C262), M286 (≠ I263), Y447 (≠ F425), Y455 (= Y433)
Sites not aligning to the query:
Query Sequence
>WP_092992765.1 NCBI__GCF_900102855.1:WP_092992765.1
MAFTSVNPATGKRLAEFAYWDAEKLDSVLQQVADATPGWAATPVKGRALLIRRLGETLRE
QREQLAAIITREMGKLIGEARGEIDKCAWLCDYYAESGPGFLADETLESDATRSLVAYQP
LGTVLAVMPWNFPFWQVFRFAVPGLLAGNTGLLKHASNVPQCAKSIENLFIEAGFPEGVF
RSLMIGASQVEGVIADLRVQAVTLTGSDSAGRKVAAAAGAHLKKSVLELGGSDPFVVLED
ADLDGAARAAVTSRFLNGGQSCIAAKRFILVDAVAENFLARFKAGVEALVPGDPMTEQAT
LPPMARTDLRDDLHRQVIASIEQGAEAVTGCRPLEGEGAFYAASILDRVEPGMPAYSEEL
FGPVAIVLRARDEADALRIANDSPFGLGGSVWSADTQRGEAFARALECGCAFVNGMVKSD
PRLPFGGIKQSGYGRELSHHGIREFVNAKTIWIR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory