SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_092996972.1 NCBI__GCF_900102855.1:WP_092996972.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3ijrF 2.05 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD+
51% identity, 82% coverage: 56:330/334 of query aligns to 12:286/290 of 3ijrF

query
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active site: G57 (= G100), S182 (= S225), L192 (= L235), Y195 (= Y238), K199 (= K242), K240 (≠ G283)
binding magnesium ion: D55 (= D98), S56 (= S99), E80 (= E123)
binding nicotinamide-adenine-dinucleotide: P21 (= P64), D55 (= D98), S56 (= S99), G57 (= G100), I58 (= I101), Y77 (= Y120), L78 (= L121), E80 (= E123), G103 (= G146), D104 (= D147), L105 (≠ V148), N131 (= N174), V132 (≠ A175), A133 (= A176), Q134 (≠ F177), I155 (≠ T198), T180 (= T223), S182 (= S225), Y195 (= Y238), K199 (= K242), P225 (= P268), G226 (= G269), P227 (= P270), I228 (≠ V271), T230 (= T273), L232 (= L275)

3i3oA 2.06 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD-acetone
51% identity, 82% coverage: 56:330/334 of query aligns to 4:278/282 of 3i3oA

query
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active site: G49 (= G100), S174 (= S225), L184 (= L235), Y187 (= Y238), K191 (= K242), K232 (≠ G283)
binding magnesium ion: D47 (= D98), S48 (= S99), E72 (= E123)
binding nicotinamide adenine dinucleotide acetone adduct: G45 (= G96), D47 (= D98), S48 (= S99), G49 (= G100), I50 (= I101), Y69 (= Y120), L70 (= L121), E72 (= E123), G95 (= G146), D96 (= D147), L97 (≠ V148), N123 (= N174), V124 (≠ A175), A125 (= A176), Q126 (≠ F177), Q127 (= Q178), I147 (≠ T198), T172 (= T223), S174 (= S225), Y187 (= Y238), K191 (= K242), P217 (= P268), G218 (= G269), I220 (≠ V271), T222 (= T273), L224 (= L275)

5jydB Crystal structure of a putative short chain dehydrogenase from burkholderia cenocepacia
50% identity, 84% coverage: 51:330/334 of query aligns to 9:289/292 of 5jydB

query
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active site: G58 (= G100), S184 (= S225), L194 (= L235), Y197 (= Y238), K201 (= K242), P242 (= P282)
binding magnesium ion: D56 (= D98), S57 (= S99), E82 (= E123)
binding nicotinamide-adenine-dinucleotide: G54 (= G96), D56 (= D98), S57 (= S99), G58 (= G100), I59 (= I101), L79 (= L121), E82 (= E123), D106 (= D147), I107 (≠ V148), N133 (= N174), A134 (= A175), A135 (= A176), T182 (= T223), S184 (= S225), Y197 (= Y238), K201 (= K242), P227 (= P268), G228 (= G269), P229 (= P270), Y230 (≠ V271), T232 (= T273), L234 (= L275), Q235 (≠ N276)

3r3sA Structure of the ygha oxidoreductase from salmonella enterica
41% identity, 82% coverage: 57:329/334 of query aligns to 15:288/292 of 3r3sA

query
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active site: G58 (= G100), S184 (= S225), L194 (= L235), Y197 (= Y238), K201 (= K242), Q242 (≠ P282)
binding magnesium ion: D56 (= D98), S57 (= S99), E82 (= E123)
binding nicotinamide-adenine-dinucleotide: D56 (= D98), S57 (= S99), G58 (= G100), I59 (= I101), L79 (= L121), E82 (= E123), D106 (= D147), L107 (≠ V148), V133 (≠ N174), A134 (= A175), G135 (≠ A176), S184 (= S225), Y197 (= Y238), K201 (= K242), P227 (= P268), G228 (= G269), I230 (≠ V271), T232 (= T273), L234 (= L275), Q235 (≠ N276)

P0AG84 Uncharacterized oxidoreductase YghA; EC 1.-.-.- from Escherichia coli (strain K12) (see paper)
40% identity, 82% coverage: 57:329/334 of query aligns to 17:290/294 of P0AG84

query
sites
P0AG84

P

P

E

K

Q

Q

H

K

L

Q

E

P

K

T

P

P

G

G

P

I

E

Q

S

A

E

K

L

M

D

T

P

P

R

V

P

P

E

D

Y

C

L

G

A

E

P
x
K

E

T

Y

Y

K

V

G

G

S

S

G

G

K

R

L

L

R

K

G

D

M

R

K

K

A

A

L

L

I

V

T

T

G

G

D

D

S

S

G

G

I

I

G

G

R

R

A

A

V

A

A

A

V

I

L

A

F

Y

A

A

R

R

E

E

E

G

A

A

D

D

I

V

A

A

I

I

L

S

Y

Y

L

L

-

P

N

V

E

E

D

E

E

E

D

D

A

A

R

Q

E

D

T

V

K

K

S

K

A

I

V

I

E

E

N

E

E

C

G

G

G

R

K

K

C

A

L

V

E

L

I

L

A

P

G

G

D

D

V

L

K

S

A

D

P

E

A

K

F

F

C

A

R

R

Y

S

A

L

V

V

E

H

R

E

T

A

L

H

E

K

E

A

Y

L

G

G

Q

G

L

L

D

D

I

I

L

M

V

A

N

L

N

V

A

A

A

G

F

K

Q

Q

E

V

H

A

A

I

A

P

S

D

L

I

E

A

E

D

I

L

S

T

E

S

E

E

H

Q

F

F

D

Q

E

K

T

T

L

F

R

A

T

I

N

N

L

V

Y

F

G

A

Y

L

F

F

H

W

M

L

A

T

K

Q

A

E

A

A

V

I

P

P

H

L

L

L

K

P

P

K

G

G

S

A

S

S

I

I

N

T

T

T

G

S

S

S

E

I

T

Q

G

A

I

Y

F

Q

G

P

Q

S

P

P

V

H

L

L

D

D

Y

Y

S

A

L

A

T

T

K

K

G

A

I

I

H

L

A

N

F

Y

T

S

K

R

A

G

L

L

A

A

T

K

N

Q

L

V

I

A

P

E

K

K

E

G

I

I

R

R

V

V

N

N

A

I

V

V

A

A

P

P

G

G

P

P

V

I

W

W

T

T

P

A

L

L

N

Q

-

I

P

S

A

G

D

G

R

Q

P

T

P

Q

G

D

T

K

L

I

S

P

K

Q

F

F

G

G

A

Q

T

Q

T

T

D

P

M

M

K

K

R

R

P

A

A

G

Q

Q

P

P

E

A

E

E

V

L

S

A

P

P

A

V

Y

Y

V

V

F

Y

L

L

A

A

A

S

P

Q

S

E

C

S

S

S

G

-

Y

Y

I

V

T

T

G

A

T

E

V

V

I

H

P

G

V

V

M

C

G

G

K39 (≠ P79) modified: N6-acetyllysine

7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
36% identity, 73% coverage: 86:330/334 of query aligns to 8:252/253 of 7v0hG

query
sites
7v0hG

K

K

L

L

R

A

G

G

M

K

K

V

A

A

L

I

I

V

T

T

G
|
G

G

A

D
x
S

S
x
K

G
|
G

I
|
I

G

G

R

A

A

A

V

I

A

A

V

K

L

A

F

L

A

A

R

D

E

E

E

G

A

A

D

A

I

V

A

V

I

V

L

N

Y

Y

L
x
A

N
x
S

E
x
S

D

K

E

A

D

G

A

A

R

D

E

A

T

V

K

V

S

S

A

A

V

I

E

T

N

E

E

A

G

G

K

R

C

A

L

V

E

A

I

V

A

G

G
|
G

D
|
D

V
|
V

K

S

A

K

P

A

A

A

F

D

C

A

R

Q

Y

R

A

I

V

V

E

D

R

T

T

A

L

I

E

E

E

T

Y

Y

G

G

Q

R

L

L

D

D

I

V

L

L

V

V

N

N

N
|
N

A
x
S

A
x
G

F

V

Q
x
Y

E

E

H

F

A

A

A

-

S

P

L

I

E

E

E

A

I

I

S

T

E

E

H

H

F

Y

D

R

E

R

T

Q

L

F

R

D

T

T

N

N

L

V

Y

F

G

G

Y

V

F

L

H

L

M

T

A

T

K

Q

A

A

V

V

P

K

H

H

L

L

K

G

P

E

G

G

S

A

S

S

I

I

N

N

T
x
I

G

S

S
|
S

E

V

T

V

G

T

I

S

F

I

G

T

Q

P

P

P

V

A

L

S

L

A

D

V

Y
|
Y

S

S

L

G

T

T

K
|
K

G

G

G

A

I

V

H

D

A

A

F

I

T

T

K

G

A

V

L

L

A

A

T

L

N

E

L

L

I

G

P

P

K

R

E

K

I

I

R

R

V

V

N

N

A

A

V

I

A

N

P
|
P

G
|
G

P
x
M

V
x
I

W

V

T
|
T

P

E

L
x
G

N
x
T

P

H

A

S

D

A

R

G

P
x
I

P

I

G

G

T

S

-

D

-

L

L

E

S

A

K

Q

F

V

G

L

A

G

T

Q

T

T

D

P

M

L

K

G

R

R

P

L

A

G

Q

E

P

P

E

N

E

D

V

I

S

A

P

S

A

V

Y

A

V

V

F

F

L

L

A

A

A

S

P

D

S

D

C

A

S

R

G

-

Y

W

I

M

T

T

G

G

T

E

V

H

I

L

P

V

V

V

M

S

G

G

V

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G96), S20 (≠ D98), K21 (≠ S99), G22 (= G100), I23 (= I101), A43 (≠ L121), S44 (≠ N122), S45 (≠ E123), G68 (= G146), D69 (= D147), V70 (= V148), N96 (= N174), S97 (≠ A175), G98 (≠ A176), Y100 (≠ Q178), I144 (≠ T223), S146 (= S225), Y159 (= Y238), K163 (= K242), P189 (= P268), G190 (= G269), M191 (≠ P270), I192 (≠ V271), T194 (= T273), G196 (≠ L275), T197 (≠ N276)
binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S225), Y159 (= Y238), M191 (≠ P270), I202 (≠ P281)

3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
34% identity, 73% coverage: 87:331/334 of query aligns to 11:257/267 of 3ay6B

query
sites
3ay6B

L

L

R

K

G

D

M

K

K

V

A

V

L

V

I

I

T

T

G
|
G

G

G

D

S

S
x
T

G
|
G

I
x
L

G

G

R

R

A

A

V

M

A

A

V

V

L

R

F

F

A

G

R

Q

E

E

A

A

D

K

I

V

A

V

I

I

L

N

Y

Y

L
x
Y

N

N

E

N

D

E

E

E

D

E

A

A

R

L

E

D

T

A

K

K

S

K

A

E

V

V

E

E

N

E

E

A

G

G

K

Q

C

A

L

I

E

I

I

V

A

Q

G

G

D
|
D

V
|
V

K

T

A

K

P

E

A

E

F

D

C

V

R

V

Y

N

A

L

V

V

E

Q

R

T

T

A

L

I

E

K

E

E

Y

F

G

G

Q

T

L

L

D

D

I

V

L

M

V

I

N

N

N
|
N

A
|
A

A
x
G

F
x
V

Q
x
E

E

N

H

P

A

V

A

P

S

S

L

-

E

H

E

E

I

L

S

S

E

L

E

D

H

N

F

W

D

N

E

K

T

V

L

I

R

D

T

T

N

N

L

L

Y

T

G

G

Y

A

F

F

H

L

M

G

A

S

K

R

A

E

A

A

V

I

P

K

H

Y

L

F

K

V

P

E

G

N

S

D

-

I

-

K

-

G

S

N

I

V

I

I

N

N

T
x
M

G

S

S
|
S

E

V

T
x
H

G

E

I

M

F

I

G

P

Q
x
W

P

P

V

L

L

F

L

V

D

H

Y
|
Y

S

A

L

A

T

S

K
|
K

G

G

I

M

H

K

A

L

F

M

T

T

K

E

A

T

L

L

A

A

T

L

N

E

L

Y

I

A

P

P

K

K

E

G

I

I

R

R

V

V

N

N

A

N

V

I

A

G

P
|
P

G
|
G

P

A

V
x
M

W

N

T
|
T

P
|
P

L
x
I

N
|
N

P

A

-

E

-
x
K

-

F

A

A

D

D

R

-

P

-

P

P

G

V

T

Q

L

R

S

A

K

D

F

V

G

E

A

S

T

M

T

I

D

P

M

M

K

G

R

Y

P

I

A

G

Q

K

P

P

E

E

V

V

S

A

P

A

A

V

Y

A

V

A

F

F

L

L

A

A

A

S

P

S

S

Q

C

A

S

S

G

-

Y

Y

I

V

T

T

G

G

T

I

V

T

I

L

P

F

V

A

M

D

G

G

V

M

T

T

active site: G24 (= G100), S151 (= S225), Y164 (= Y238), K168 (= K242)
binding beta-D-glucopyranose: E102 (≠ Q178), S151 (= S225), H153 (≠ T227), W158 (≠ Q232), Y164 (= Y238), N202 (= N276), K205 (vs. gap)
binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G96), T23 (≠ S99), G24 (= G100), L25 (≠ I101), Y45 (≠ L121), D71 (= D147), V72 (= V148), N98 (= N174), A99 (= A175), G100 (≠ A176), V101 (≠ F177), M149 (≠ T223), S151 (= S225), Y164 (= Y238), K168 (= K242), P194 (= P268), G195 (= G269), M197 (≠ V271), T199 (= T273), P200 (= P274), I201 (≠ L275), N202 (= N276)

8bcjB Crystal structure of short-chain dehydrogenase pa3128 from pseudomonas aeruginosa pao1 in complex with NADP+
35% identity, 71% coverage: 93:329/334 of query aligns to 8:249/250 of 8bcjB

query
sites
8bcjB

L

L

I

I

T

T

G
|
G

G

A

D
x
S

S
x
R

G
|
G

I
|
I

G

G

R

A

A

A

V

T

A

A

V

L

L

L

F

A

A

A

R

E

E

R

E

G

A

Y

D

A

I

V

A

V

I

L

L

N

Y

Y

L
|
L

N
x
R

E
x
N

D

R

E

E

D

A

A

A

R

E

E

A

T

L

K

R

S

Q

A

R

V

I

E

E

N

R

E

Q

G

G

K

E

C

A

L

L

E

A

I

V

A

A

G
x
A

D
|
D

V
|
V

K

A

A

E

P

E

A

G

F

D

C

V

R

E

Y

R

A

L

V

F

E

A

R

S

T

I

L

D

E

E

E

R

Y

F

G

G

Q

R

L

L

D

D

I

V

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

F

M

Q

L

E

E

H

A

A

Q

A

T

S

R

L

L

E

E

E

N

I

I

S

D

E

A

H

R

F

L

D

H

E

R

T

V

L

F

R

A

T
|
T

N

N

L

V

Y

T

G

G

Y

S

F

F

H

L

M

C

A

A

K

R

A

E

A

A

V

V

P

K

H

R

L

L

K

S

P

T

-

R

-

H

-

G

-

R

G

G

S

G

S

S

I

I

I

V

N

N

T
x
V

G

S

S
|
S

E

M

T

A

G

S

I

R

F

L

G

G

Q

S

P

P

V

N

-

E

L

Y

L

I

D

D

Y
|
Y

S

A

L

A

T

A

K
|
K

G

G

G

A

I

I

H

D

A

S

F

M

T

T

K

I

A

G

L

L

A

A

T

R

N

E

L

V

I

A

P

A

K

E

E

G

I

I

R

R

V

V

N

N

A

A

V

V

A

R

P
|
P

G
|
G

P

L

V
x
I

W

D

T
|
T

P

E

L
x
I

N
x
H

P

A

A

S

D

G

R

G

P

E

P

P

G

G

T

R

L

I

S

E

K

R

F

L

G

K

A

G

T

G

T

I

D

P

M

L

K

G

R

R

P

G

A

G

Q

T

P

A

E

E

V

V

S

A

P

R

A

A

Y

I

V

L

F

W

L

L

A

A

A

S

P

D

S

E

C

A

S

S

G

-

Y

Y

I

S

T

T

G

G

T

T

V

F

I

I

P

D

V

V

M

S

G

G

binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G96), S13 (≠ D98), R14 (≠ S99), G15 (= G100), I16 (= I101), L36 (= L121), R37 (≠ N122), N38 (≠ E123), A61 (≠ G146), D62 (= D147), V63 (= V148), N89 (= N174), A90 (= A175), G91 (≠ A176), T113 (= T198), V143 (≠ T223), S145 (= S225), Y159 (= Y238), K163 (= K242), P189 (= P268), G190 (= G269), I192 (≠ V271), T194 (= T273), I196 (≠ L275), H197 (≠ N276)

5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
34% identity, 72% coverage: 92:331/334 of query aligns to 10:253/255 of 5itvA

query
sites
5itvA

A

V

L

L

I

I

T

T

G
|
G

G

G

D

A

S
|
S

G
|
G

I
|
I

G

G

R

Y

A

A

V

A

A

V

V

Q

L

A

F

F

A

L

R

G

E

Q

A

A

D

N

I

V

A

V

I

V

L

-

Y

-

L

-

N

-

E

A

D
|
D

E
x
I

D

D

A

E

R

A

E

Q

T

G

K

E

S

A

A

M

V

V

E

R

N

K

E

E

G

N

K

D

C

R

L

L

E

H

-

F

I

V

A

Q

G
x
T

D

D

V
x
I

K

T

A

D

P

E

A

A

F

A

C

C

R

Q

Y

H

A

A

V

V

E

E

R

S

T

A

L

V

E

H

E

T

Y

F

G

G

Q

G

L

L

D

D

I

V

L

L

V

I

N

N

N
|
N

A

A

A
x
G

F

I

Q

-

E

E

H

I

A

V

A

A

S

P

L

I

E

H

E

E

I

M

S

E

E

L

E

S

H

D

F

W

D

N

E

K

T

V

L

L

R

Q

T

V

N

N

L

L

Y

T

G

G

Y

M

F

F

H

L

M

M

A

S

K

K

A

H

A

A

V

L

P

K

H

H

L

M

K

L

P

A

G

A

S

G

-

K

-

G

S

N

I

I

N

N

T
|
T

G

C

S
|
S

E

V

T

G

G

G

I

L

F

V

G

A

Q

W

P

P

V

D

L

I

L

P

D

A

Y
|
Y

S

N

L

A

T

S

K
|
K

G

G

I

V

H

L

A

Q

F

L

T

T

K

K

A

S

L

M

A

A

T

V

N

D

L

Y

I

A

P

K

K

H

E

Q

I

I

R

R

V

V

N

N

A

C

V

V

A

C

P
|
P

G
|
G

P
x
I

V
x
I

W

D

T

T

P

P

L

L

N

N

P

E

-

K

-

S

-

F

A

L

D

E

R

N

P

N

P

E

G

G

T

T

L

L

S

E

K

E

F

I

-

K

-

E

-

K

G

A

A

K

T

V

T

N

D

P

M

L

K

L

R

R

P

L

A

G

Q

K

P

P

E

E

V

I

S

A

P

N

A

V

Y

M

V

L

F

F

L

L

A

A

A

S

P

-

S

D

C

L

S

S

G

S

Y

Y

I

M

T

T

G

G

T

S

V

A

I

I

P

T

V

A

M

D

G

G

V

Y

T

T

active site: G18 (= G100), S141 (= S225), Y154 (= Y238), K158 (= K242)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G96), S17 (= S99), G18 (= G100), I19 (= I101), D38 (= D124), I39 (≠ E125), T61 (≠ G146), I63 (≠ V148), N89 (= N174), G91 (≠ A176), T139 (= T223), S141 (= S225), Y154 (= Y238), K158 (= K242), P184 (= P268), G185 (= G269), I186 (≠ P270), I187 (≠ V271)

8w0oA Gdh-105 crystal structure
32% identity, 73% coverage: 87:331/334 of query aligns to 5:251/259 of 8w0oA

query
sites
8w0oA

L

L

R

K

G

G

M

K

K

V

A

V

L

A

I

I

T

T

G
|
G

G

A

D

A

S
|
S

G
|
G

I
x
L

G

G

R

K

A

A

V

M

A

A

V

I

L

R

F

F

A

G

R

K

E

E

E

Q

A

A

D

K

I

V

A

V

I

I

L

N

Y

Y

L
x
Y

N

S

E

N

D

K

E
x
Q

D

D

A

P

R

N

E

E

T

V

K

K

S

E

A

E

V

V

E

I

N

K

E

A

G

G

K

E

C

A

L

V

E

V

I

V

A

Q

G

G

D
|
D

V
|
V

K

T

A

K

P

E

A

E

F

D

C

V

R

K

Y

N

A

I

V

V

E

Q

R

T

T

A

L

I

E

K

E

E

Y

F

G

G

Q

T

L

L

D

D

I

I

L

M

V

I

N

N

N
|
N

A
|
A

A
x
G

F

L

Q

E

E

N

H

P

A

V

A

P

S

S

L

-

E

H

E

E

I

M

S

P

E

L

E

K

H

D

F

W

D

D

E

K

T

V

L

I

R

G

T

T

N

N

L

L

Y

T

G

G

Y

A

F

F

H

L

M

G

A

S

K

R

A

E

A

A

V

I

P

K

H

Y

L

F

K

V

P

E

G

N

S

D

-

I

-

K

-

G

S

N

I

V

I

I

N

N

T
x
M

G

S

S

S

E

V

T

H

G

E

I

V

F

I

G

P

Q

W

P

P

V

L

L

F

L

V

D

H

Y
|
Y

S

A

L

A

T

S

K
|
K

G

G

I

M

H

K

A

L

F

M

T

T

K

K

A

T

L

L

A

A

T

L

N

E

L

Y

I

A

P

P

K

K

E

G

I

I

R

R

V

V

N

N

A

N

V

I

A

G

P
|
P

G
|
G

P

A

V
x
I

W

N

T
|
T

P

T

L

I

N

N

P

A

-

E

-

K

-

F

A

A

D

D

R

-

P

-

P

P

G

K

T

Q

L

K

S

A

K

D

F

V

G

E

A

S

T

M

T

I

D

P

M

M

K

G

R

Y

P

I

A

G

Q

E

P

P

E

E

V

I

S

A

P

A

A

V

Y

A

V

A

F

W

L

L

A

A

A

S

P

K

S

E

C

A

S

S

G

-

Y

Y

I

V

T

T

G

G

T

I

V

T

I

L

P

F

V

A

M

D

G

G

V

M

T

T

binding nicotinamide-adenine-dinucleotide: G14 (= G96), S17 (= S99), G18 (= G100), L19 (≠ I101), Y39 (≠ L121), Q43 (≠ E125), D65 (= D147), V66 (= V148), N92 (= N174), A93 (= A175), G94 (≠ A176), M143 (≠ T223), Y158 (= Y238), K162 (= K242), P188 (= P268), G189 (= G269), I191 (≠ V271), T193 (= T273)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 73% coverage: 86:330/334 of query aligns to 4:242/244 of 4nbuB

query
sites
4nbuB

K

R

L

L

R

Q

G

D

M

K

K

V

A

A

L

I

I

I

T

T

G
|
G

G

A

D

A

S
x
N

G
|
G

I
|
I

G

G

R

L

A

E

V

A

A

A

V

R

L

V

F

F

A

M

R

K

E

E

E

G

A

A

D

K

I

V

A

V

I

I

L

A

Y
x
D

L
x
F

N

N

E

E

D

A

E

A

D

G

A

-

R

-

E

-

T

-

K

K

S

E

A

A

V

V

E

E

N

A

E

N

G

P

G

G

K

V

C

V

L

F

E

-

I

I

A

R

G
x
V

D
|
D

V
|
V

K

S

A

D

P

R

A

E

F

S

C

V

R

H

Y

R

A

L

V

V

E

E

R

N

T

V

L

A

E

E

E

R

Y

F

G

G

Q

K

L

I

D

D

I

I

L

L

V

I

N

N

N
|
N

A
|
A

A
x
G

F
x
I

Q

T

E

R

H
x
D

A

S

A

M

S

-

L

L

E

S

E
x
K

I

M

S

T

E

V

E

D

H

Q

F

F

D

Q

E

Q

T

V

L

I

R

N

T

V

N
|
N

L

L

Y

T

G

G

Y

V

F

F

H

H

M

C

A

T

K

Q

A

A

A

V

V

L

P

P

H

Y

L

M

-

A

-

E

K

Q

P

G

G

K

S

G

S

K

I

I

N

N

T
|
T

G

S

S
|
S

E

V

T

T

G

G

I

T

F

Y

G

G

Q
x
N

P
x
V

V

G

L
x
Q

L

T

D

N

Y
|
Y

S

A

L

A

T

A

K
|
K

G

A

G

G

I

V

H

I

A

G

F

M

T

T

K

K

A

T

L

W

A

A

T

K

N

E

L

L

I

A

P

R

K

K

E

G

I

I

R

N

V

V

N

N

A

A

V

V

A

A

P
|
P

G

G

P
x
F

V
x
T

W

E

T
|
T

P

A

L
x
M

N

-

P

V

A

A

D

E

R

V

P

P

P

E

G

K

T

V

L

I

S

E

K
|
K

F

M

G

K

A

A

T

Q

T

V

D

P

M

M

K

G

R

R

P

L

A

G

Q

K

P

P

E

E

E

D

V

I

S

A

P

N

A

A

Y

Y

V

L

F

F

L

L

A

A

A

S

P

H

S

E

C

-

S

S

G

D

Y

Y

I

V

T

N

G

G

T

H

V

V

I

L

P

H

V

V

M

D

G

G

V

I

active site: G18 (= G100), N111 (= N199), S139 (= S225), Q149 (≠ L235), Y152 (= Y238), K156 (= K242)
binding acetoacetyl-coenzyme a: D93 (≠ H180), K98 (≠ E186), S139 (= S225), N146 (≠ Q232), V147 (≠ P233), Q149 (≠ L235), Y152 (= Y238), F184 (≠ P270), M189 (≠ L275), K200 (= K287)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G96), N17 (≠ S99), G18 (= G100), I19 (= I101), D38 (≠ Y120), F39 (≠ L121), V59 (≠ G146), D60 (= D147), V61 (= V148), N87 (= N174), A88 (= A175), G89 (≠ A176), I90 (≠ F177), T137 (= T223), S139 (= S225), Y152 (= Y238), K156 (= K242), P182 (= P268), F184 (≠ P270), T185 (≠ V271), T187 (= T273), M189 (≠ L275)

P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
30% identity, 73% coverage: 87:331/334 of query aligns to 5:251/261 of P40288

query
sites
P40288

L

L

R

E

G

G

M

K

K

V

A

V

L
x
V

I
|
I

T
|
T

G
|
G

G
x
S

D
x
S

S
x
T

G
|
G

I
x
L

G
|
G

R
x
K

A
x
S

V
x
M

A
|
A

V
x
I

L
x
R

F
|
F

A
|
A

R
x
T

E
|
E

E
x
K

A
|
A

D
x
K

I
x
V

A
x
V

I

V

L

N

Y

Y

L

R

N

S

E

K

D

E

E

D

D

E

A

A

R

N

E

S

T

V

K

L

S

E

A

E

V

I

E

K

N

K

E

V

G

G

K

E

C

A

L

I

E

A

I

V

A

K

G

G

D

D

V

V

K

T

A

V

P

E

A

S

F

D

C

V

R

I

Y

N

A

L

V

V

E

Q

R

S

T

A

L

I

E

K

E

E

Y

F

G

G

Q

K

L

L

D

D

I

V

L

M

V

I

N

N

A

A

A

G

F

L

Q

-

E
|
E

H

N

A

P

A

V

S

S

L

S

E

H

E

E

I

M

S

S

E

L

E

S

H
x
D

F

W

D

N

E

K

T
x
V

L

I

R

D

T

T

N

N

L

L

Y

T

G

G

Y

A

F

F

H

L

M

G

A

S

K

R

A

E

A

A

V

I

P

K

H

Y

L

F

K

V

P
x
E

G

N

S

D

-

I

-

K

-

G

S

T

I

V

I

I

N

N

T

M

G

S

S

S

E

V

T

H

G

E

I

K

F

I

G

P

Q

W

P

P

V

L

L

F

L

V

D

H

Y

Y

S

A

L

A

T

S

K

K

G

G

I

M

H

K

A

L

F

M

T

T

K

E

A

T

L

L

A

A

T

L

N

E

L

Y

I

A

P

P

K

K

E

G

I

I

R

R

V
|
V

N

N

A

N

V

I

A

G

P

P

G

G

P

A

V

I

W

N

T

T

P
|
P

L

I

N

N

P

A

-

E

-

K

-

F

A

A

D

D

R

-

P

-

P

P

G

E

T

Q

L

R

S

A

K

D

F

V

G
x
E

A

S

T

M

T

I

D

P

M

M

K

G

R
x
Y

P

I

A

G

Q

E

P

P

E

E

V

I

S

A

P

A

A

V

Y

A

V

A

F

W

L

L

A

A

A

S

P

S

S

E

C

A

S

S

G

-

Y

Y

I

V

T

T

G

G

T

I

V

T

I

L

P

F

V

A

M

D

G

G

V

M

T

T

11:35 (vs. 93:117, 40% identical) binding NADP(+)
E96 (= E179) mutation E->A,G,K: Heat stable.
D108 (≠ H191) mutation to N: Heat stable.
V112 (≠ T195) mutation to A: Heat stable.
E133 (≠ P216) mutation to K: Heat stable.
V183 (= V263) mutation to I: Heat stable.
P194 (= P274) mutation to Q: Heat stable.
E210 (≠ G289) mutation to K: Heat stable.
Y217 (≠ R296) mutation to H: Heat stable.

Sites not aligning to the query:

252 Q→L: Heat stable.
253 Y→C: Heat stable.
258 A→G: Heat stable.

5wuwA Serratia marcescens short-chain dehydrogenase/reductase f98l/f202l mutant (see paper)
34% identity, 73% coverage: 87:331/334 of query aligns to 3:244/245 of 5wuwA

query
sites
5wuwA

L

L

R

Q

G

G

M

K

K

V

A

A

L

F

I

V

T

Q

G
|
G

G

G

D

S

S
x
R

G
|
G

I
|
I

G

G

R

A

A

A

V

I

A

V

V

K

L

R

F

L

A

A

R

S

E

E

E

G

A

A

D

A

I

V

A

A

I

F

L

T

Y
|
Y

L
x
A

N
x
A

E

S

D

A

E

D

D

R

A

A

R

E

E

A

T

V

K

A

S

S

A

A

V

V

E

T

N

T

E

A

G

G

K

K

C

V

L

L

E

A

I

I

A

K

G

A

D
|
D

V
x
S

K

A

A

D

P

A

A

A

F

A

C

L

R

Q

Y

Q

A

A

V

V

E

R

R

Q

T

A

L

V

E

S

E

H

Y

F

G

G

Q

N

L

L

D

D

I

I

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

F

V

Q

L

E

T

H

L

A

G

S

T

L

-

E

E

I

L

S

A

E

L

E

D

H

D

F

L

D

D

E

R

T

M

L

L

R

A

T

V

N

N

L

V

Y

R

G

S

Y

V

F

F

H

V

M

A

A

S

K

Q

A

E

A

A

V

A

P

R

H

H

L

M

K

N

P

D

G

G

S

G

S

R

I

I

N

H

T

I

G

G

S
|
S

-

T

-

N

-

A

E

E

T

R

G

V

I

P

F

F

G

G

Q

G

P

A

V

A

L

V

L

-

D

-

Y
|
Y

S

A

L

M

T

S

K
|
K

G

S

G

A

I
x
L

H

V

A

G

F

L

T

T

K

K

A

G

L

M

A

A

T

R

N

D

L

L

I

G

P

P

K

R

E

S

I

I

R

T

V

V

N

N

A

N

V

V

A

Q

P

P

G
|
G

P
|
P

V
|
V

W

D

T

T

P

E

L

M

N

N

P

P

A

-

D

-

R

-

P

D

P

A

G

G

T

E

L

L

S

A

-

D

K

Q

F

L

G

K

A

Q

T

L

T

M

D

A

M

I

K

G

R

R

P

Y

A

G

Q

K

P

D

E

E

V

I

S

A

P

G

A

F

Y

V

V

A

F

Y

L

L

A

A

A

G

P

P

S

Q

C

A

S

-

G

G

Y

Y

I

I

T

T

G

G

T

A

V

S

I

L

P

S

V

I

M

D

G

G

V

F

T

S

active site: G16 (= G100), S140 (= S225), Y154 (= Y238), L161 (≠ I245)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G96), R15 (≠ S99), I17 (= I101), Y36 (= Y120), A37 (≠ L121), A38 (≠ N122), D63 (= D147), S64 (≠ V148), N90 (= N174), A91 (= A175), G92 (≠ A176), Y154 (= Y238), K158 (= K242), G185 (= G269), P186 (= P270), V187 (= V271)

1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
29% identity, 73% coverage: 87:331/334 of query aligns to 5:251/261 of 1g6kA

query
sites
1g6kA

L

L

R

E

G

G

M

K

K

V

A

V

L

V

I

I

T

T

G

G

G

S

D

S

S
x
T

G
|
G

I
x
L

G

G

R

K

A

S

V

M

A

A

V

I

L

R

F

F

A

A

R

T

E

E

E

K

A

A

D

K

I

V

A

V

I

V

L

N

Y

Y

L
x
R

N

S

E

K

D

E

E

D

D

E

A

A

R

N

E

S

T

V

K

L

S

E

A

E

V

I

E

K

N

K

E

V

G

G

K

E

C

A

L

I

E

A

I

V

A

K

G

G

D
|
D

V
|
V

K

T

A

V

P

E

A

S

F

D

C

V

R

I

Y

N

A

L

V

V

E

Q

R

S

T

A

L

I

E

K

E

E

Y

F

G

G

Q

K

L

L

D

D

I

V

L

M

V

I

N

N

N
|
N

A
|
A

A
x
G

F

L

Q

A

E

N

H

P

A

V

A

S

S

S

L

-

E

H

E

E

I

M

S

S

E

L

E

S

H

D

F

W

D

N

E

K

T

V

L

I

R

D

T

T

N

N

L

L

Y

T

G

G

Y

A

F

F

H

L

M

G

A

S

K

R

A

E

A

A

V

I

P

K

H

Y

L

F

K

V

P

E

G

N

S

D

-

I

-

K

-

G

S

T

I

V

I

I

N

N

T
x
M

G

S

S
|
S

E

V

T

H

G

E

I

K

F

I

G

P

Q

W

P

P

V

L

L

F

L

V

D

H

Y
|
Y

S

A

L

A

T

S

K
|
K

G

G

I

M

H

K

A

L

F

M

T

T

K

E

A

T

L

L

A

A

T

L

N

E

L

Y

I

A

P

P

K

K

E

G

I

I

R

R

V

V

N

N

A

N

V

I

A

G

P
|
P

G
|
G

P

A

V
x
I

W

N

T
|
T

P

P

L

I

N

N

P

A

-

E

-

K

-

F

A

A

D

D

R

-

P

-

P

P

G

E

T

Q

L

R

S

A

K

D

F

V

G

E

A

S

T

M

T

I

D

P

M

M

K

G

R

Y

P

I

A

G

Q

E

P

P

E

E

V

I

S

A

P

A

A

V

Y

A

V

A

F

W

L

L

A

A

A

S

P

S

S

E

C

A

S

S

G

-

Y

Y

I

V

T

T

G

G

T

I

V

T

I

L

P

F

V

A

M

D

G

G

V

M

T

T

active site: G18 (= G100), S145 (= S225), Y158 (= Y238), K162 (= K242)
binding nicotinamide-adenine-dinucleotide: T17 (≠ S99), G18 (= G100), L19 (≠ I101), R39 (≠ L121), D65 (= D147), V66 (= V148), N92 (= N174), A93 (= A175), G94 (≠ A176), M143 (≠ T223), S145 (= S225), Y158 (= Y238), P188 (= P268), G189 (= G269), I191 (≠ V271), T193 (= T273)

4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
33% identity, 73% coverage: 86:330/334 of query aligns to 2:237/239 of 4nbtA

query
sites
4nbtA

K

K

L

L

R

E

G

G

M

K

K

V

A

A

L

V

I

I

T

T

G
|
G

G

G

D

A

S
x
K

G
|
G

I
x
L

G

G

R

Q

A

A

V

I

A

A

V

L

L

A

F

Y

A

A

R

E

E

E

E

G

A

A

D

K

I

V

A

I

I

-

L

-

Y

-

L

-

N

-

E

-

D

A

E

G

D
|
D

A
x
L

R

G

E

D

T

L

K

T

S

Y

A

S

V

H

E

P

N

N

E

V

G

E

G

G

K

M

C

Y

L
|
L

E
x
N

I
x
V

A

T

G

D

D

V

V

T

K

G

A

V

P

E

A

K

F

F

C

Y

R

-

Y

-

A

-

V

-

E

Q

R

S

T

V

L

I

E

D

E

K

Y

Y

G

G

Q

K

L

I

D

D

I

I

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

F

I

Q

T

E

K

H

D

A

A

A

M

S

T

L

-

E

R

E

K

I

M

S

T

E

E

E

A

H

Q

F

W

D

D

E

A

T

V

L

I

R

D

T

V

N

N

L

L

Y

K

G

G

Y

V

F

F

H

N

M

L

A

T

K

R

A

L

A

V

V

G

P

P

H

Q

L

M

K

Q

P

T

G

N

-

G

-

Y

S

G

S

S

I

I

N

N

T
x
I

G

S

S
|
S

E

V

T

V

G

G

I

V

F

F

G

G

Q

N

P

I

V

G

L

Q

L

A

D

N

Y
|
Y

S

A

L

A

T

T

K
|
K

G

A

G

G

I

V

H

I

A

G

F

L

T

T

K

M

A

T

L

W

A

A

T

K

N

E

L

F

I

A

P

L

K

K

-

G

-

A

E

N

I

V

R

R

V

V

N

N

A

A

V

I

A

A

P
|
P

G
|
G

P

Y

V
x
I

W

M

T
|
T

P

D

L

I

N

-

P

L

A

K

D

T

R

V

P

P

P

Q

G

D

T

L

L

L

S

D

K

K

F

F

G

A

A

A

T

L

T

T

D

M

M

L

K

N

R

R

P

L

A

G

Q

Q

P

P

E

E

V

I

S

A

P

K

A

V

Y

A

V

L

F

F

L

L

A

A

A

S

P

D

S

D

C

A

S

S

G

-

Y

Y

I

V

T

T

G

G

T

Q

V

T

I

I

P

N

V

V

M

N

G

G

V

M

active site: G16 (= G100), S132 (= S225), Y145 (= Y238), K149 (= K242)
binding nicotinamide-adenine-dinucleotide: G12 (= G96), K15 (≠ S99), G16 (= G100), L17 (≠ I101), D36 (= D126), L37 (≠ A127), L52 (= L142), N53 (≠ E143), V54 (≠ I144), N80 (= N174), A81 (= A175), G82 (≠ A176), I130 (≠ T223), S132 (= S225), Y145 (= Y238), K149 (= K242), P177 (= P268), G178 (= G269), I180 (≠ V271), T182 (= T273)

3iccA Crystal structure of a putative 3-oxoacyl-(acyl carrier protein) reductase from bacillus anthracis at 1.87 a resolution (see paper)
33% identity, 73% coverage: 87:329/334 of query aligns to 5:252/255 of 3iccA

query
sites
3iccA

L

L

R

K

G

G

M

K

K

V

A

A

L

L

I

V

T

T

G
|
G

G

A

D
x
S

S
x
R

G
|
G

I
|
I

G

G

R

R

A

A

V

I

A

A

V

K

L

R

F

L

A

A

R

N

E

D

E

G

A

A

D

L

I

V

A

A

I

I

L
x
H

Y
|
Y

L
x
G

N

N

E

R

D

K

E

E

D

E

A

A

R

E

E

E

T

T

K

V

S

Y

A

E

V

I

E

Q

N

S

E

N

G

G

K

S

C

A

L

F

E

S

I

I

A

G

G

A

D

N

V
x
L

K
x
E

A

S

P

L

A

H

F

G

C

V

R

E

Y

-

A

A

V

L

E

Y

R

S

T

S

L

L

E

D

E

N

Y

E

G

L

Q

Q

-

N

-

R

-

T

-

G

-

S

-

T

-

K

L

F

D

D

I

I

L

L

V

I

N

N

N
|
N

A

A

A
x
G

F

I

Q

G

E

P

H

-

A

G

A

A

S

F

L

I

E

E

I

T

S

T

E

E

E

Q

H

F

F

F

D

D

E

R

T

M

L

V

R

S

T

V

N

N

L

A

Y

K

G

A

Y

P

F

F

H

F

M

I

A

I

K

Q

A

Q

A

A

V

L

P

S

H

R

L

L

K

R

P

D

G

N

S

S

S

R

I

I

N

N

T
x
I

G

S

S
|
S

E

A

T

A

G

T

I

R

F

I

G

S

Q

L

P

P

V

D

L
x
F

L

I

D

A

Y
|
Y

S

S

L

M

T

T

K
|
K

G

G

G

A

I

I

H

N

A

T

F

M

T

T

K

F

A

T

L

L

A

A

T

K

N

Q

L

L

I

G

P

A

K

R

E

G

I

I

R

T

V

V

N

N

A

A

V

I

A

L

P
|
P

G
|
G

P

F

V

V

W

K

T

T

P

D

L
x
M

N
|
N

P

A

A

E

D

L

R

L

P

S

P

D

G

P

T

M

L

M

S

K

K

Q

F

Y

G

A

A

T

T

T

T

I

D

S

-

A

M

F

K

N

R

R

P

L

A

G

Q

E

P

V

E

E

E

D

V

I

S

A

P

D

A

T

Y

A

V

A

F

F

L

L

A

A

A

S

P

P

S

D

C

-

S

S

G

R

Y

W

I

V

T

T

G

G

T

Q

V

L

I

I

P

D

V

V

M

S

G

G

active site: G18 (= G100), S148 (= S225), F158 (≠ L235), Y161 (= Y238), K165 (= K242)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G96), S16 (≠ D98), R17 (≠ S99), G18 (= G100), I19 (= I101), H37 (≠ L119), Y38 (= Y120), G39 (≠ L121), L66 (≠ V148), E67 (≠ K149), N98 (= N174), G100 (≠ A176), I146 (≠ T223), S148 (= S225), Y161 (= Y238), K165 (= K242), P191 (= P268), G192 (= G269), M198 (≠ L275), N199 (= N276)

4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
34% identity, 74% coverage: 86:331/334 of query aligns to 3:247/249 of 4bmsF

query
sites
4bmsF

K

R

L

L

R

L

G

N

M

K

K

T

A

A

L

V

I

I

T

T

G
|
G

G

G

D
x
N

S
|
S

G

G

I
|
I

G

G

R

L

A

A

V

T

A

A

V

K

L

R

F

F

A

V

R

A

E

E

E

G

A

A

D

Y

I

V

A

F

I

I

L

V

Y

-

L

-

N

-

E

-

D

G

E
x
R

D
x
R

A

R

R

K

E

E

T

L

K

E

S

Q

A

A

V

A

E

A

N

E

E

I

G

G

G

R

K

N

C

V

L

T

E

A

I

V

A

K

G
x
A

D
|
D

V
|
V

-

T

K

K

A

L

P

E

A

D

F

L

C

D

R

R

-

L

Y

Y

A

A

V

I

E

V

R

R

T

-

L

-

E

E

E

Q

Y

R

G

G

Q

S

L

I

D

D

I

V

L

L

V

F

N

A

N
|
N

A
x
S

A
x
G

F

A

Q

I

E

E

H

Q

A

K

A

-

S

T

L

L

E

E

I

I

S

T

E

P

E

E

H

H

F

Y

D

D

E

R

T

T

L

F

R

D

T
x
V

N

N

L

V

Y

R

G

G

Y

L

F

I

H

F

M

T

A

V

K

Q

A

K

A

A

V

L

P

P

H

L

L

L

K

R

P

D

G

G

S

G

S

S

I

V

I

I

N

L

T

T

G

S

S
|
S

E

V

T

A

G

G

I

V

F

L

G

G

Q

L

P

Q

V

A

L
x
H

L

D

D

T

Y
|
Y

S

S

L

A

T

A

K
|
K

G

A

I

V

H

R

A

S

F

L

T

A

K

R

A

T

L

W

A

T

T

T

N

E

L

L

I

K

P

G

K

R

E

S

I

I

R

R

V

V

N

N

A

A

V

V

A

S

P

P

G
|
G

P

A

V
x
I

W

D

T
|
T

P

P

L
x
I

-

I

-

E

-

N

-

Q

-

V

N

S

P

T

A
x
Q

D

E

R

E

P

A

P

D

G

E

T

L

L

R

S

A

K

K

F

F

G

A

A

A

T

A

T

T

D

P

M

L

K

G

R

R

P

V

A

G

Q

R

P

P

E

E

V

L

S

A

P

A

A

A

Y

V

V

L

F

F

L

L

A

A

A

S

P

D

S

D

C

S

S

S

G

-

Y

Y

I

V

T

A

G

G

T

I

V

E

I

L

P

F

V

V

M

D

G

G

V

L

T

T

active site: S137 (= S225), H147 (≠ L235), Y150 (= Y238), K154 (= K242), Q195 (≠ A278)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G96), N15 (≠ D98), S16 (= S99), I18 (= I101), R38 (≠ E125), R39 (≠ D126), A59 (≠ G146), D60 (= D147), V61 (= V148), N87 (= N174), S88 (≠ A175), G89 (≠ A176), V110 (≠ T198), S137 (= S225), Y150 (= Y238), K154 (= K242), G181 (= G269), I183 (≠ V271), T185 (= T273), I187 (≠ L275)

4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
32% identity, 73% coverage: 87:331/334 of query aligns to 5:252/254 of 4fn4A

query
sites
4fn4A

L

L

R

K

G

N

M

K

K

V

A

V

L

I

I

V

T

T

G
|
G

G

A

D

G

S
|
S

G
|
G

I
|
I

G

G

R

R

A

A

V

I

A

A

V

K

L

K

F

F

A

A

R

L

E

N

E

D

A

S

D

I

I

V

A

V

I

A

L

V

Y
x
E

L
|
L

N

L

E

E

D

D

E
x
R

D

-

A

L

R

N

E

Q

T

I

K

V

S

Q

A

E

V

L

E

R

N

G

E

M

G

G

G

K

K

E

C

V

L

L

E

G

I

V

A

K

G
x
A

D
|
D

V
|
V

K

S

A

K

P

K

A

K

F

D

C

V

R

E

Y

E

A

F

V

V

E

R

R

R

T

T

L

F

E

E

E

T

Y

Y

G

S

Q

R

L

I

D

D

I

V

L

L

V

C

N

N

N
|
N

A

A

A
x
G

F
x
I

Q

M

E

D

H

G

A

V

A

T

S

P

L

V

E

A

E

E

I

V

S

S

E

D

E

E

H

L

F

W

D

E

E

R

T

V

L

L

R

A

T

V

N

N

L

L

Y

Y

G

S

Y

A

F

F

H

Y

M

S

A

S

K

R

A

A

A

V

V

I

P

P

-

I

H

M

L

L

K

K

P

Q

G

G

S

K

S

G

-

V

I

I

I

V

N

N

T
|
T

G

A

S
|
S

E

I

T

A

G

G

I

I

F

R

G

G

Q

G

P

F

V

A

L

G

L

A

D

P

Y
|
Y

S

T

L

V

T

A

K
|
K

G

H

G

G

I

L

H

I

A

G

F

L

T

T

K

R

A

S

L

I

A

A

T

A

N

H

L

Y

I

G

P

D

K

Q

E

G

I

I

R

R

V

A

N

V

A

A

V

V

A

L

P
|
P

G

G

P

T

V
|
V

W

K

T
|
T

P
x
N

L
x
I

N

G

P

L

A

G

D

S

R

S

P

K

P

P

G
x
S

T

E

L

L

S

G

K

M

F

R

G

T

A

L

T

T

T

K

D

L

M

M

-

S

-

L

-

S

K

S

R

R

P

L

A

A

Q

E

P

P

E

E

E

D

V

I

S

A

P

N

A

V

Y

I

V

V

F

F

L

L

A

A

A

S

P

D

S

E

C

A

S

S

G

-

Y

F

I

V

T

N

G

G

T

D

V

A

I

V

P

V

V

V

M

D

G

G

V

L

T

T

active site: G18 (= G100), S144 (= S225), Y157 (= Y238), K161 (= K242), S202 (≠ G283)
binding nicotinamide-adenine-dinucleotide: G14 (= G96), S17 (= S99), G18 (= G100), I19 (= I101), E38 (≠ Y120), L39 (= L121), R43 (≠ E125), A63 (≠ G146), D64 (= D147), V65 (= V148), N91 (= N174), G93 (≠ A176), I94 (≠ F177), T142 (= T223), S144 (= S225), Y157 (= Y238), K161 (= K242), P187 (= P268), V190 (= V271), T192 (= T273), N193 (≠ P274), I194 (≠ L275)

2d1yA Crystal structure of tt0321 from thermus thermophilus hb8 (see paper)
34% identity, 75% coverage: 85:333/334 of query aligns to 1:239/240 of 2d1yA

query
sites
2d1yA

G

G

K

L

L

F

R

A

G

G

M

K

K

G

A

V

L

L

I

V

T

T

G
|
G

G

G

D

A

S
x
R

G
|
G

I
|
I

G

G

R

R

A

A

V

I

A

A

V

Q

L

A

F

F

A

A

R

R

E

E

E

G

A

A

D

L

I

V

A

A

I

L

L

C

Y
x
D

L
|
L

N
x
R

E

P

D

-

E

-

D

-

A

-

R

-

E

E

T

G

K

K

S

E

A

V

V

A

E

E

N

A

E

I

G

G

K

A

C

F

L

F

E

Q

I
x
V

A

-

G

-

D
|
D

V
x
L

K

E

A

D

P

E

A

R

F

E

C

R

R

V

Y

R

A

F

V

V

E

E

R

E

T

A

L

A

E

Y

E

A

Y

L

G

G

Q

R

L

V

D

D

I

V

L

L

V

V

N

N

N
|
N

A
|
A

F
x
I

Q

A

E

A

H

P

A

G

A

S

S

A

L

L

E

T

E

-

I

V

S

R

E

L

E

P

H

E

F

W

D

R

E

R

T

V

L

L

R

E

T

V

N

N

L

L

Y

T

G

A

Y

P

F

M

H

H

M

L

A

S

K

A

A

L

A

A

V

A

P

R

H

E

L

M

K

R

P

K

-

V

-

G

G

G

S

G

S

A

I

I

I

V

N

N

T
x
V

G

A

S
|
S

E

V

T

Q

G

G

I

L

F

F

G

A

Q

E

P

Q

V

E

L
x
N

L

A

D

A

Y
|
Y

S

N

L

A

T

S

K
|
K

G

G

I

L

H

V

A

N

F

L

T

T

K

R

A

S

L

L

A

A

T

L

N

D

L

L

I

A

P

P

K

L

E

R

I

I

R

R

V

V

N

N

A

A

V

V

A

A

P
|
P

G
|
G

P

A

V
x
I

W

A

T
|
T

P

E

-
x
A

-
x
V

L

L

N

E

P

A

A

I

D

A

R

R

P

-

G

-

T

-

L

-

S

R

K

D

F

W

G

E

A

D

T

L

T

H

D

A

M

L

K

R

R

R

P

L

A

G

Q

K

P

P

E

E

V

V

S

A

P

E

A

A

Y

V

V

L

F

F

L

L

A

A

A

S

P

E

S

K

C

A

S

S

G

-

Y

F

I

I

T

T

G

G

T

A

V

I

I

L

P

P

V

V

M

D

G

G

V

M

T

T

G

A

S

S

active site: G16 (= G100), S135 (= S225), N145 (≠ L235), Y148 (= Y238), K152 (= K242)
binding nicotinamide-adenine-dinucleotide: G12 (= G96), R15 (≠ S99), I17 (= I101), D36 (≠ Y120), L37 (= L121), R38 (≠ N122), V55 (≠ I144), D56 (= D147), L57 (≠ V148), N83 (= N174), A84 (= A175), A85 (= A176), I86 (≠ F177), V133 (≠ T223), S135 (= S225), Y148 (= Y238), K152 (= K242), P178 (= P268), G179 (= G269), I181 (≠ V271), T183 (= T273), A185 (vs. gap), V186 (vs. gap)

5u2wA Crystal structure of a short chain dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP
34% identity, 73% coverage: 86:329/334 of query aligns to 3:243/246 of 5u2wA

query
sites
5u2wA

K

R

L

L

R

Q

G

G

M

K

K

R

A

A

L

L

I

V

T

T

G
|
G

G

G

D
x
S

S
x
R

G
|
G

I
|
I

G

G

R

A

A

A

V

I

A

A

V

K

L

R

F

L

A

A

R

A

E

D

E

G

A

A

D

D

I

V

A

A

I

I

L

T

Y
|
Y

L
x
E

N
x
K

E
x
S

D

A

E

E

D

R

A

A

R

Q

E

A

T

V

K

V

S

A

A

G

V

I

E

E

N

A

E

L

G

G

G

R

K

R

C

A

L

I

E

A

I

I

A

Q

G
x
A

D
|
D

V
x
S

K

A

A

D

P

P

A

V

F

A

C

V

R

R

Y

N

A

A

V

V

E

D

R

R

T

V

L

A

E

E

E

A

Y

F

G

G

Q

G

L

L

D

D

I

I

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

F

I

Q

-

E

F

H

R

A

A

A

G

S

S

L

L

E

D

I

L

S

T

E

L

E

D

H

D

F

I

D

D

E

A

T

T

L

L

R

N

T

V

N

N

L

V

Y

R

G

A

Y

V

F

I

H

V

M

A

A

S

K

Q

A

A

V

A

P

R

H

H

L

L

K

G

P

E

G

G

S

G

S

R

I

I

I

V

N

S

T
|
T

G

G

S
|
S

-

C

-

L

E

A

T

T

G

R

I

V

F

P

G

D

Q

A

P

G

V
x
M

L

S

L

L

D

-

Y
|
Y

S

A

L

A

T

S

K
|
K

G

A

I

L

H

I

A

G

F

W

T

T

K

Q

A

G

L

L

A

A

T

R

N

D

L

L

I

G

P

P

K

R

E

G

I

I

R

T

V

V

N

N

A

I

V

V

A

H

P
|
P

G
|
G

P

S

V
x
T

W

D

T
|
T

P

D

L
x
M

N
|
N

P

P

A

A

D

D

R

-

P

-

G

-

T

-

L

-

S

-

K

-

F

-

G

G

A

A

T

H

T

A

D

D

M

A

K

Q

R

R

P

S

-

R

-

M

-

A

-

I

-

Q

-

Y

A

G

Q

K

P

A

E

D

V

V

S

A

P

A

A

L

Y

V

V

A

F

F

L

V

A

V

A

G

P

P

S

E

C

G

S

R

G

-

Y

S

I

I

T

N

G

G

T

T

V

G

I

L

P

T

V

I

M

D

G

G

active site: G17 (= G100), S141 (= S225), M152 (≠ V234), Y155 (= Y238), K159 (= K242)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G96), S15 (≠ D98), R16 (≠ S99), G17 (= G100), I18 (= I101), Y37 (= Y120), E38 (≠ L121), K39 (≠ N122), S40 (≠ E123), A63 (≠ G146), D64 (= D147), S65 (≠ V148), N91 (= N174), A92 (= A175), G93 (≠ A176), T139 (= T223), Y155 (= Y238), K159 (= K242), P185 (= P268), G186 (= G269), T188 (≠ V271), T190 (= T273), M192 (≠ L275), N193 (= N276)

Query Sequence

>WP_092996972.1 NCBI__GCF_900102855.1:WP_092996972.1
MAQGREEQFRQAAEKAAERQRNIQRAMDEHDQRSKGGGNRDSAIQAGSHGYPEPPLPEQH
LEKPGPESELDPRPEYLAPEYKGSGKLRGMKALITGGDSGIGRAVAVLFAREEADIAILY
LNEDEDARETKSAVENEGGKCLEIAGDVKAPAFCRYAVERTLEEYGQLDILVNNAAFQEH
AASLEEISEEHFDETLRTNLYGYFHMAKAAVPHLKPGSSIINTGSETGIFGQPVLLDYSL
TKGGIHAFTKALATNLIPKEIRVNAVAPGPVWTPLNPADRPPGTLSKFGATTDMKRPAQP
EEVSPAYVFLAAPSCSGYITGTVIPVMGGVTGSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory