SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_093393428.1 NCBI__GCF_900114975.1:WP_093393428.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 4:346/346 of Q04797

query
sites
Q04797

G

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S98 (= S99) modified: Phosphoserine
Y146 (≠ V147) modified: Phosphotyrosine

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
56% identity, 97% coverage: 6:336/341 of query aligns to 5:335/337 of P23247

query
sites
P23247

F

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C132 (= C131) active site, Acyl-thioester intermediate

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
56% identity, 97% coverage: 6:336/341 of query aligns to 4:334/336 of 2r00C

query
sites
2r00C

F

F

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active site: C131 (= C131), Q158 (= Q158), R237 (= R237), H244 (= H244)
binding 2,2'-oxydiacetic acid: V15 (= V17), N95 (= N97), C131 (= C131), N315 (= N317)

3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/358 of 3q11A

query
sites
3q11A

G

G

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D

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A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), T75 (≠ V79), G160 (= G164), M161 (≠ K165), G162 (≠ K166)
binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237)

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/359 of 4r5hA

query
sites
4r5hA

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

S

S

V
x
T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q
|
Q

S

A

V

V

S

S

G
|
G

T

A

G
|
G

K
x
M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ A77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), K222 (= K215), R244 (= R237), H251 (= H244)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (≠ V79), N93 (= N97), T94 (≠ S98), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G321)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/359 of 4r4jA

query
sites
4r4jA

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A

S

S

S

V
x
T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q
|
Q

S

A

V

V

S

S

G
|
G

T

A

G
|
G

K
x
M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237), H251 (= H244)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (≠ V79), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G321)

3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/359 of 3pyxB

query
sites
3pyxB

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A

S

S

S

V
x
T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q

Q

S

A

V

V

S
|
S

G
|
G

T

A

G
|
G

K
x
M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N
|
N

I
x
L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R102), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (≠ V79), C127 (= C131), S157 (= S161), G158 (= G162), G160 (= G164), M161 (≠ K165), N324 (= N317), L325 (≠ I318)

3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/361 of 3pylC

query
sites
3pylC

G

G

F

Y

R

T

V

V

A

A

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A

A

S

S

A

A

R

R

S

S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L

T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

A

S

S

S

V

T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q

Q

S

A

V

V

S

S

G
|
G

T

A

G

G

K

M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding 3-amino-D-alanine: N126 (= N130), C127 (= C131), G158 (= G162), E219 (= E212), R244 (= R237)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/357 of 4r54A

query
sites
4r54A

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

S

S

V
x
T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q

Q

S

A

V

V

S

S

G

G

T

A

G
|
G

K

M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R

R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G76), S73 (≠ A77), T94 (≠ S98), S95 (= S99), R98 (= R102), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (≠ V79), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), G328 (= G321)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/357 of 4r41A

query
sites
4r41A

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

S

S

V

T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q

Q

S

A

V

V

S

S

G

G

T

A

G
|
G

K
x
M

K
x
G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R

R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S74), G72 (= G76), S73 (≠ A77), N93 (= N97), T94 (≠ S98), S95 (= S99), R98 (= R102), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), G160 (= G164), M161 (≠ K165), G162 (≠ K166)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/357 of 4r3nA

query
sites
4r3nA

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R

R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

S

S

V

T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q
|
Q

S

A

V

V

S

S

G

G

T

A

G
|
G

K
x
M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ A77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G321)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/357 of 3q1lA

query
sites
3q1lA

G

G

F

Y

R

T

V

V

A

A

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A

A

S

S

A

A

R

R

S

S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L

T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

A

S

S

S

V

T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q
|
Q

S

A

V

V

S

S

G
|
G

T

A

G

G

K

M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G

-

D

-

K

-

H

H

Y

P

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

G

D

S

V

F

F

L

T

E

D

N

N

G

D

F

Y

S

T

E

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

F

M

E

E

D

D

P

D

D

S

I

I

Q

A

V

V

C

S

A

A

T

T

T

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

V

I

E

E

L

T

K

K

G

E

L

V

L

A

T

P

V

I

E

E

V

V

R

K

R

A

L

A

L

I

E

A

K

A

A

F

P

P

G

G

V

A

V

V

L

L

V

E

D

D

N

D

P

V

D

A

R

H

A

Q

E

I

Y

Y

P

P

L

Q

P

A

L

I

N

N

A

A

E

V

G

G

R

S

D

R

E

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

P

L

S

D

V

A

E

E

N

K

G

G

I

I

V

H

M

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

V

H

K

E

E

R

G

G

L

L

L

V

active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
binding 2-amino-ethanethiol: N126 (= N130), C127 (= C131), G158 (= G162), E219 (= E212)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 1:346/357 of 3pwsA

query
sites
3pwsA

G

G

F

Y

R

T

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

N

A

M

Q

M

M

V

I

R

K

V

M

L

L

E

E

R

S

N

T

F

L

P

P

V

I

K

D

S

K

L

I

K

R

L

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

K

K

T

S

L

L

S

K

F

F

R

K

G

D

E

Q

E

D

V

I

P

T

V

I

Q

E

E

E

L
x
T

T

T

E

E

D

T

S

A

F

F

R

E

D

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

A

S

S

S

V
x
T

S

S

R

A

K

K

F

Y

A

A

P

P

L

Y

A

A

A

V

S

K

Q

A

G

G

C

V

V

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

Q

H

A

A

L

L

R

D

N

A

H

H

K

N

K

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

Q

R

D

Q

A

K

V

W

G

G

I

L

K

D

R

R

I

I

V

I

V

V

T

S

T

T

F

Y

Q

Q

S

A

V

V

S

S

G
|
G

T

A

G
|
G

K
x
M

K

G

A

A

I

I

F

L

E

E

L

T

E

Q

Q

R

Q

E

V

L

R

R

G

E

W

V

V

L

E

N

G

D

K

G

A

V

V

K

P

P

P

C

S

D

V

L

Y

H

P

A

-

E

-

I

-

L

-

P

-

S

-

G