SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_093394992.1 NCBI__GCF_900114975.1:WP_093394992.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
32% identity, 94% coverage: 6:276/287 of query aligns to 2:267/290 of 5mr0D

query
sites
5mr0D

L

L

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Y

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Y

W

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active site: F32 (= F36), G34 (≠ T38), K150 (= K159), E183 (= E192), L206 (= L215)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R55), G100 (= G112), L101 (= L113), K150 (= K159), Y154 (= Y163), E183 (= E192), G186 (≠ A195), D187 (≠ S196), L206 (= L215), I209 (≠ V218), T210 (= T219), G245 (≠ N254), T246 (≠ S255)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
31% identity, 95% coverage: 5:276/287 of query aligns to 2:268/290 of 5e25A

query
sites
5e25A

D

E

L

L

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L

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W

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active site: F33 (= F36), G35 (≠ T38), K151 (= K159), E184 (= E192), L207 (= L215)
binding 2-oxoglutaric acid: Y88 (≠ I98), K151 (= K159), T247 (≠ S255), A248 (≠ M256)
binding pyridoxal-5'-phosphate: R52 (= R55), K151 (= K159), Y155 (= Y163), E184 (= E192), G187 (≠ A195), D188 (≠ S196), L207 (= L215), G209 (= G217), I210 (≠ V218), T211 (= T219), G246 (≠ N254), T247 (≠ S255)

3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
31% identity, 93% coverage: 10:276/287 of query aligns to 5:262/277 of 3daaA

query
sites
3daaA

W

W

N

N

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G

active site: Y31 (≠ F36), V33 (≠ T38), K145 (= K159), E177 (= E192), L201 (= L215)
binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F36), R50 (= R55), K145 (= K159), E177 (= E192), S180 (≠ A195), S181 (= S196), L201 (= L215), G203 (= G217), I204 (≠ V218), T205 (= T219), S240 (≠ N254), T241 (≠ S255)

2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
31% identity, 93% coverage: 10:276/287 of query aligns to 5:262/277 of 2daaA

query
sites
2daaA

W

W

N

N

G

D

R

Q

F

I

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V

D

N

E

A

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G

active site: Y31 (≠ F36), V33 (≠ T38), K145 (= K159), E177 (= E192), L201 (= L215)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F36), V33 (≠ T38), R50 (= R55), R98 (vs. gap), H100 (≠ L113), K145 (= K159), E177 (= E192), S180 (≠ A195), S181 (= S196), N182 (≠ S197), L201 (= L215), G203 (= G217), I204 (≠ V218), T205 (= T219), T241 (≠ S255)

1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
31% identity, 93% coverage: 10:276/287 of query aligns to 5:262/277 of 1daaA

query
sites
1daaA

W

W

N

N

G

D

R

Q

F

I

E

V

H

K

V

D

N

E

A

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D

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G

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D

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x
Y

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V

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K

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K

N

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M

L

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F

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E

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H

H

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R

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K

A

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S

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L

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E

Y

L

T

R

K

T

-

F

-

L

-

D

D

A

K

Y

F

E

H

T

-

A

-

H

Q

I

L

V

L

H

H

D

E

L

L

I

V

R

E

R

K

N

N

G

E

L

L

E

-

D

-

R

N

I

T

C

G

R

H

L

I

K

Y

I

F

I

Q

V

V

T

T

R

R

G

G

V

T

A

S

D

P

-

R

G

A

L

H

G

Q

L

F

P

P

E

E

A

N

T

T

A

V

P

K

T

P

V

V

I

I

M

I

M

G

V

Y

F

T

P

K

Y

E

N

N

P

P

P

R

N

P

P

L

E

E

E

N

Y

L

E

E

V

K

G

G

W

V

K

K

A

A

I

T

T

F

L

V

R

E

H

D

I

I

F

R

T

W

P

L

F

R

C

C

A

D

R

-

Y

I

K
|
K

T

S

L

L

N

N

Y

L

L

L

V

G

F

A

L

V

M

L

A

A

R

K

E

Q

E

E

A

A

R

H

R

E

R

K

G

G

A

C

Q

Y

E

E

A

A

L

I

L

L

V

-

D

H

R

R

E

N

H

T

L

V

A

T

E
|
E

G

G

A

S

A
x
S

S
|
S

S

N

L

V

L

F

A

G

F

I

K

K

D

D

G

G

-

I

F

L

W

Y

I

T

E

H

P

P

V

A

T

N

S

N

W

M

K

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

I

R

A

D

K

V

V

V

I

I

E

A

F

C

L

A

K

N

E

E

M

I

G

N

E

M

K

P

V

V

S

K

K

E

M

I

R

P

F

F

S

T

V

T

E

H

D

E

L

A

R

L

K

K

C

M

D

D

T

E

L

L

W

F

I

V

T

T

N
x
S

S
x
T

M

T

I

S

G

E

V

I

M

T

P

P

L

V

S

I

S

E

V

I

D

D

D

G

R

K

K

L

L

I

P

R

D

D

L

G

K

K

A

V

G

G

active site: Y31 (≠ F36), V33 (≠ T38), K145 (= K159), E177 (= E192), L201 (= L215)
binding pyridoxal-5'-phosphate: R50 (= R55), K145 (= K159), E177 (= E192), S180 (≠ A195), S181 (= S196), L201 (= L215), G203 (= G217), I204 (≠ V218), T205 (= T219), S240 (≠ N254), T241 (≠ S255)

3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
31% identity, 93% coverage: 10:276/287 of query aligns to 5:262/280 of 3lqsA

query
sites
3lqsA

W

W

N

N

G

D

R

Q

F

I

E

V

H

K

V

D

N

E

A

E

V

V

R

K

I

I

S

D

P

K

L

E

D

D

R

R

G

G

L

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

V

V

R

K

V

V

E

Y

K

N

G

G

R

E

A

M

L

F

F

T

L

V

E

N

E

E

H

H

L

I

N

D

R
|
R

L

L

A

Y

K

A

S

S

L

A

E

E

E

K

L

I

R

R

I

I

N

-

P

-

N

T

L

I

P

P

E

Y

L

T

R

K

T

-

F

-

L

-

D

D

A

K

Y

F

E

H

T

-

A

-

H

Q

I

L

V

L

H

H

D

E

L

L

I

V

R

E

R

K

N

N

G

E

L

L

E

-

D

-

R

N

I

T

C

G

R

H

L

I

K

Y

I

F

I

Q

V

V

T

T

R

R

G

G

V

T

A

S

D

P

-

R

G

A

L

H

G

Q

L

F

P

P

E

E

A

N

T

T

A

V

P

K

T

P

V

V

I

I

M

I

M

G

V

Y

F

T

P

K

Y

E

N

N

P

P

P

R

N

P

P

L

E

E

E

N

Y

L

E

E

V

K

G

G

W

V

K

K

A

A

I

T

T

F

L

V

R

E

H

D

I

I

F

R

T

W

P

L

F

R

C

C

A

D

R

-

Y

I

K
|
K

T

S

L

L

N

N

Y

L

L

L

V

G

F

A

L

V

M

L

A

A

R

K

E

Q

E

E

A

A

R

H

R

E

R

K

G

G

A

C

Q

Y

E

E

A

A

L

I

L

L

V

-

D

H

R

R

E

N

H

T

L

V

A

T

E
|
E

G

G

A

S

A
x
S

S
|
S

S
x
N

L

V

L

F

A

G

F

I

K

K

D

D

G

G

-

I

F

L

W

Y

I

T

E

H

P

P

V

A

T

N

S

N

W

M

K

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

I

R

A

D

K

V

V

V

I

I

E

A

F

C

L

A

K

N

E

E

M

I

G

N

E

M

K

P

V

V

S

K

K

E

M

I

R

P

F

F

S

T

V

T

E

H

D

E

L

A

R

L

K

K

C

M

D

D

T

E

L

L

W

F

I

V

T

T

N
x
S

S
x
T

M
x
T

I

S

G

E

V

I

M

T

P

P

L

V

S

I

S

E

V

I

D

D

D

G

R

K

K

L

L

I

P

R

D

D

L

G

K

K

A

V

G

G

active site: Y31 (≠ F36), V33 (≠ T38), K145 (= K159), E177 (= E192), L201 (= L215)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ T38), R50 (= R55), E177 (= E192), S180 (≠ A195), S181 (= S196), N182 (≠ S197), L201 (= L215), G203 (= G217), I204 (≠ V218), T205 (= T219), S240 (≠ N254), T241 (≠ S255), T242 (≠ M256)

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
31% identity, 93% coverage: 10:276/287 of query aligns to 6:263/283 of P19938

query
sites
P19938

W

W

N

N

G

D

R

Q

F

I

E

V

H

K

V

D

N

E

A

E

V

V

R

K

I

I

S

D

P

K

L

E

D

D

R

R

G

G

L

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T

V

V

V

R

K

V

V

E

Y

K

N

G

G

R

E

A

M

L

F

F

T

L

V

E

N

E

E

H

H

L

I

N

D

R
|
R

L

L

A

Y

K

A

S

S

L

A

E

E

E

K

L

I

R

R

I

I

N

-

P

-

N

T

L

I

P

P

E

Y

L

T

R

K

T

-

F

-

L

-

D

D

A

K

Y

F

E

H

T

-

A

-

H

Q

I

L

V

L

H

H

D

E

L

L

I

V

R

E

R

K

N

N

G

E

L

L

E

-

D

-

R

N

I

T

C

G

R

H

L

I

K

Y

I

F

I

Q

V

V

T

T

R

R

G

G

V

T

A

S

D

P

-
x
R

G

A

L
x
H

G

Q

L

F

P

P

E

E

A

N

T

T

A

V

P

K

T

P

V

V

I

I

M

I

M

G

V

Y

F

T

P

K

Y

E

N

N

P

P

P

R

N

P

P

L

E

E

E

N

Y

L

E

E

V

K

G

G

W

V

K

K

A

A

I

T

T

F

L

V

R

E

H

D

I

I

F

R

T

W

P

L

F

R

C

C

A

D

R

-

Y

I

K
|
K

T

S

L

L

N

N

Y

L

L

L

V

G

F

A

L

V

M

L

A

A

R

K

E

Q

E

E

A

A

R

H

R

E

R

K

G

G

A

C

Q

Y

E

E

A

A

L

I

L

L

V

-

D

H

R

R

E

N

H

T

L

V

A

T

E
|
E

G

G

A

S

S

S

S

N

L

V

L

F

A

G

F

I

K

K

D

D

G

G

-

I

F

L

W

Y

I

T

E

H

P

P

V

A

T

N

S

N

W

M

K

I

L
|
L

P

K

G

G

V

I

T

T

I

R

A

D

K

V

V

V

I

I

E

A

F

C

L

A

K

N

E

E

M

I

G

N

E

M

K

P

V

V

S

K

K

E

M

I

R

P

F

F

S

T

V

T

E

H

D

E

L

A

R

L

K

K

C

M

D

D

T

E

L

L

W

F

I

V

T

T

N

S

S

T

M

T

I

S

G

E

V

I

M

T

P

P

L

V

S

I

S

E

V

I

D

D

D

G

R

K

K

L

L

I

P

R

D

D

L

G

K

K

A

V

G

G

Y32 (≠ F36) binding substrate
R51 (= R55) binding pyridoxal 5'-phosphate
R99 (vs. gap) binding substrate
H101 (≠ L113) binding substrate
K146 (= K159) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
E178 (= E192) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
L202 (= L215) mutation to A: Inactivates enzyme.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
30% identity, 93% coverage: 10:276/287 of query aligns to 5:262/282 of 1a0gB

query
sites
1a0gB

W

W

N

N

G

D

R

Q

F

I

E

V

H

K

V

D

N

E

A

E

V

V

R

K

I

I

S

D

P

K

L

E

D

D

R

R

G

G

L

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

V

V

R

K

V

V

E

Y

K

N

G

G

R

E

A

M

L

F

F

T

L

V

E

N

E

E

H

H

L

I

N

D

R
|
R

L

L

A

Y

K

A

S

S

L

A

E

E

E

K

L

I

R

R

I

I

N

-

P

-

N

T

L

I

P

P

E

Y

L

T

R

K

T

-

F

-

L

-

D

D

A

K

Y

F

E

H

T

-

A

-

H

Q

I

L

V

L

H

H

D

E

L

L

I

V

R

E

R

K

N

N

G

E

L

L

E

-

D

-

R

N

I

T

C

G

R

H

L

I

K

Y

I

F

I

Q

V

V

T

T

R

R

G

G

V

T

A

S

D

P

-

R

G

A

L

H

G

Q

L

F

P

P

E

E

A

N

T

T

A

V

P

K

T

P

V

V

I

I

M

I

M

G

V

Y

F

T

P

K

Y

E

N

N

P

P

P

R

N

P

P

L

E

E

E

N

Y

L

E

E

V

K

G

G

W

V

K

K

A

A

I

T

T

F

L

V

R

E

H

D

I

I

F

R

T

W

P

L

F

R

C

C

A

D

R

-

Y

I

K
|
K

T

S

L

L

N

N

Y

L

L

L

V

G

F

A

L

V

M

L

A

A

R

K

E

Q

E

E

A

A

R

H

R

E

R

K

G

G

A

C

Q

Y

E

E

A

A

L

I

L

L

V

-

D

H

R

R

E

N

H

T

L

V

A

T

E
|
E

G

G

A

S

A
x
S

S
|
S

S

N

L

V

L

F

A

G

F

I

K

K

D

D

G

G

-

I

F

L

W

Y

I

T

E

H

P

P

V

A

T

N

S

N

W

M

K

I

L
x
A

P

K

G
|
G

V
x
I

T
|
T

I

R

A

D

K

V

V

V

I

I

E

A

F

C

L

A

K

N

E

E

M

I

G

N

E

M

K

P

V

V

S

K

K

E

M

I

R

P

F

F

S

T

V

T

E

H

D

E

L

A

R

L

K

K

C

M

D

D

T

E

L

L

W

F

I

V

T

T

N
x
S

S
x
T

M

T

I

S

G

E

V

I

M

T

P

P

L

V

S

I

S

E

V

I

D

D

D

G

R

K

K

L

L

I

P

R

D

D

L

G

K

K

A

V

G

G

active site: Y31 (≠ F36), V33 (≠ T38), K145 (= K159), E177 (= E192), A201 (≠ L215)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R55), K145 (= K159), E177 (= E192), S180 (≠ A195), S181 (= S196), G203 (= G217), I204 (≠ V218), T205 (= T219), S240 (≠ N254), T241 (≠ S255)

7p3tB Transaminase of gamma-proteobacterium (see paper)
30% identity, 92% coverage: 7:270/287 of query aligns to 5:263/299 of 7p3tB

query
sites
7p3tB

I

I

V

I

W

Y

W

I

N

N

G

G

R

D

F

Y

E

L

H

P

V

L

N

S

A

Q

V

A

R

R

I

V

S

S

P

P

L

V

D

D

R

Q

G

G

L

F

Q

L

Y

L

G

G

D

D

G

G

L

V

F

F

E

D

T

V

V

V

R

S

V

A

E

W

K

K

G

G

R

N

A

I

L

F

F

K

L

L

E

D

E

A

H

H

L

L

N

D

R
|
R

L

F

A

F

K

D

S

S

L

I

E

Q

E

A

L

A

R

R

I

L

N

N

P

H

N

D

L

M

P

S

E

R

L

-

R

-

T

-

F

-

L

-

D

D

A

A

Y

W

E

K

T

E

A

A

H

I

I

I

V

-

H

-

D

E

L

T

I

T

R

R

N

N

G

G

L

L

E

D

D

D

R

-

I

-

C

A

R

S

L

I

K

R

I

F

I

I

V

V

T

T

R

R

G

G

V

E

A

P

D

K

G

G

-

V

L

V

G

A

L

D

P

P

E

R

A

D

T

F

A

K

P

P

T

T

V

C

I

I

M

V

M

W

V

V

F

A

P

P

Y

Y

N

I

P

F

P

L

N

A

P

D

E

E

-

K

-

R

-

N

-

G

-

I

-

R

-

L

-

M

-

I

-

S

Y

A

E

T

V

R

G

G

W

F

K

P

A

A

I

D

T

T

L

L

R

D

H

-

I

-

F

-

T

-

P

-

F

-

C

-

A

P

R

R

Y

Y

K
|
K

T

C

L

L

N

D

Y
x
R

L

L

V

H

F

S

L

Q

M

L

A

I

R

R

E

L

E

E

A

A

R

L

R

E

R

A

G

G

A

Y

Q

D

E

D

A

A

L

L

L

W

V

L

D

D

R

H

E

S

E

G

H

H

L

V

A

S

E
|
E

G
x
S

A
|
A

S
|
S

S

N

L

L

L

F

A

I

F

V

K

K

D

N

G

G

F

V

W

L

I

Y

E

T

P

P

V

-

T

S

S

A

W

G

K

I

L

L

P

R

G
|
G

V
x
I

T
|
T

I

R

A

D

K

T

V

I

I

L

E

E

F

L

L

A

K

T

E

E

M

L

G

D

E

I

K

P

V

W

S

K

K

E

M

R

R

Q

F

L

S

S

V

A

E

F

D

D

L

V

R

Y

K

I

C

A

D

D

T

E

L

V

W

F

I

T

T

C

N

S

S
x
T

M

A

I

G

G

G

V

A

M

L

P

P

L

V

S

R

S

E

V

V

D

A

D

G

R

R

K

T

L

I

binding pyridoxal-5'-phosphate: R53 (= R55), K153 (= K159), R157 (≠ Y163), E186 (= E192), S187 (≠ G193), A188 (= A194), A189 (= A195), S190 (= S196), G210 (= G217), I211 (≠ V218), T212 (= T219), T248 (≠ S255)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
29% identity, 94% coverage: 8:276/287 of query aligns to 5:275/304 of 1iyeA

query
sites
1iyeA

V

I

W

W

W

F

N

N

G

G

R

E

F

M

E

V

H

R

V

W

N

E

A

D

V

A

R

K

I

V

S

H

P

V

L

M

D

S

R

H

G

A

L

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

V

I

R

R

V

C

-

Y

-

D

-

S

E

H

K

K

G

G

R

P

A

V

L

V

F

F

L

R

E

H

-

R

E

E

H

H

L

M

N

Q

R
|
R

L

L

A

H

K

D

S

S

L

A

E

K

E

I

L

Y

R

R

I

F

N

-

P

P

N

V

L

S

P

Q

E

S

L

I

R

D

T

E

F

L

L

M

D

E

A

A

Y

-

E

-

T

-

A

-

H

-

I

-

V

C

H

R

D

D

L

V

I

I

R

R

R

K

N

N

G

N

L

L

E

T

D

S

R

A

I
x
Y

C

I

R

R

L

P

K

L

I

I

I

F

V

V

T

G

R

D

G

-

V

V

A

G

D

M

G

G

L

V

G

N

L

P

P

P

E

A

A

G

T

Y

A

S

P

T

T

D

V

V

I

I

M

I

M

A

V

A

F

F

P

P

Y

W

N

G

P

A

P
x
Y

-

L

N

G

P

A

E

E

E

A

Y

L

E

E

V

Q

G

G

-

I

-

D

-

A

-

M

-

V

-

S

W

W

K

N

A

R

I

A

T

A

L

P

R

N

H

T

I

I

F

-

T

-

P

P

F

T

C

A

A

A

R
x
K

Y
x
A

K

G

T

G

L

-

N

N

Y
|
Y

L

L

V

S

F

S

L

L

M

L

A

V

R

G

E

S

E

E

A

A

R

R

R

H

G

G

A

Y

Q

Q

E

E

A

G

L

I

L

A

V

L

D

D

R

V

E

N

E

G

H

Y

L

I

A

S

E
|
E

G

G

A

A

A
x
G

S
x
E

S
x
N

L

L

L

F

A

E

F

V

K

K

D

D

G

G

-

V

F

L

W

F

I

T

E

P

P

P

V

F

T

T

S

S

W

S

K

A

L
|
L

P

P

G
|
G

V
x
I

T
|
T

I

R

A

D

K

A

V

I

I

I

E

K

F

L

L

A

K

K

E

E

M

L

G

G

E

I

K

E

V

V

S

R

K

E

M

Q

R

V

F

L

S

S

V

R

E

E

D

S

L

L

R

Y

K

L

C

A

D

D

T

E

L

V

W

F

I

M

T

S

N
x
G

S
x
T

M
x
A

I

A

G

E

V

I

M

T

P

P

L

V

S

R

S

S

V

V

D

D

D

G

R

I

K

Q

L

V

P

G

D

E

L

G

K

R

A

C

G

G

active site: F33 (= F36), G35 (≠ T38), K156 (≠ R157), A157 (≠ Y158), E190 (= E192), L214 (= L215)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R55), Y92 (≠ I98), Y126 (≠ P133), K156 (≠ R157), Y161 (= Y163), E190 (= E192), G193 (≠ A195), E194 (≠ S196), N195 (≠ S197), L214 (= L215), G216 (= G217), I217 (≠ V218), T218 (= T219), G253 (≠ N254), T254 (≠ S255), A255 (≠ M256)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
29% identity, 94% coverage: 8:276/287 of query aligns to 5:275/304 of 1iydA

query
sites
1iydA

V

I

W

W

W

F

N

N

G

G

R

E

F

M

E

V

H

R

V

W

N

E

A

D

V

A

R

K

I

V

S

H

P

V

L

M

D

S

R

H

G

A

L

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

V

I

R

R

V

C

-

Y

-

D

-

S

E

H

K

K

G

G

R

P

A

V

L

V

F

F

L

R

E

H

-

R

E

E

H

H

L

M

N

Q

R
|
R

L

L

A

H

K

D

S

S

L

A

E

K

E

I

L

Y

R

R

I

F

N

-

P

P

N

V

L

S

P

Q

E

S

L

I

R

D

T

E

F

L

L

M

D

E

A

A

Y

-

E

-

T

-

A

-

H

-

I

-

V

C

H

R

D

D

L

V

I

I

R

R

R

K

N

N

G

N

L

L

E

T

D

S

R

A

I
x
Y

C

I

R

R

L

P

K

L

I

I

I

F

V

V

T

G

R

D

G

-

V

V

A

G

D

M

G

G

L

V

G

N

L

P

P

P

E

A

A

G

T

Y

A

S

P

T

T

D

V

V

I

I

M

I

M

A

V

A

F

F

P

P

Y

W

N

G

P

A

P
x
Y

-

L

N

G

P

A

E

E

E

A

Y

L

E

E

V

Q

G

G

-

I

-

D

-

A

-

M

-

V

-

S

W

W

K

N

A

R

I

A

T

A

L

P

R

N

H

T

I

I

F

-

T

-

P

P

F

T

C

A

A

A

R
x
K

Y
x
A

K

G

T

G

L

-

N

N

Y
|
Y

L

L

V

S

F

S

L

L

M

L

A

V

R

G

E

S

E

E

A

A

R

R

R

H

G

G

A

Y

Q

Q

E

E

A

G

L

I

L

A

V

L

D

D

R

V

E

N

E

G

H

Y

L

I

A

S

E
|
E

G

G

A

A

A
x
G

S
x
E

S

N

L

L

L

F

A

E

F

V

K

K

D

D

G

G

-

V

F

L

W

F

I

T

E

P

P

P

V

F

T

T

S

S

W

S

K

A

L
|
L

P

P

G
|
G

V
x
I

T
|
T

I

R

A

D

K

A

V

I

I

I

E

K

F

L

L

A

K

K

E

E

M

L

G

G

E

I

K

E

V

V

S

R

K

E

M

Q

R

V

F

L

S

S

V

R

E

E

D

S

L

L

R

Y

K

L

C

A

D

D

T

E

L

V

W

F

I

M

T

S

N

G

S
x
T

M
x
A

I

A

G

E

V

I

M

T

P

P

L

V

S

R

S

S

V

V

D

D

D

G

R

I

K

Q

L

V

P

G

D

E

L

G

K

R

A

C

G

G

active site: F33 (= F36), G35 (≠ T38), K156 (≠ R157), A157 (≠ Y158), E190 (= E192), L214 (= L215)
binding glutaric acid: Y92 (≠ I98), Y126 (≠ P133), A255 (≠ M256)
binding pyridoxal-5'-phosphate: R56 (= R55), K156 (≠ R157), Y161 (= Y163), E190 (= E192), G193 (≠ A195), E194 (≠ S196), L214 (= L215), G216 (= G217), I217 (≠ V218), T218 (= T219), T254 (≠ S255)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
29% identity, 94% coverage: 8:276/287 of query aligns to 5:275/304 of 1i1mA

query
sites
1i1mA

V

I

W

W

W

F

N

N

G

G

R

E

F

M

E

V

H

R

V

W

N

E

A

D

V

A

R

K

I

V

S

H

P

V

L

M

D

S

R

H

G

A

L

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F

F

E

E

T

G

V

I

R

R

V

C

-

Y

-

D

-

S

E

H

K

K

G

G

R

P

A

V

L

V

F

F

L

R

E

H

-

R

E

E

H

H

L

M

N

Q

R
|
R

L

L

A

H

K

D

S

S

L

A

E

K

E

I

L

Y

R

R

I

F

N

-

P

P

N

V

L

S

P

Q

E

S

L

I

R

D

T

E

F

L

L

M

D

E

A

A

Y

-

E

-

T

-

A

-

H

-

I

-

V

C

H

R

D

D

L

V

I

I

R

R

R

K

N

N

G

N

L

L

E

T

D

S

R

A

I
x
Y

C

I

R

R

L

P

K

L

I

I

I

F

V

V

T

G

R

D

G

-

V

V

A

G

D

M

G

G

L

V

G

N

L

P

P

P

E

A

A

G

T

Y

A

S

P

T

T

D

V

V

I

I

M

I

M

A

V

A

F

F

P

P

Y

W

N

G

P

A

P

Y

-

L

N

G

P

A

E

E

E

A

Y

L

E

E

V

Q

G

G

-

I

-

D

-

A

-

M

-

V

-

S

W

W

K

N

A

R

I

A

T

A

L

P

R

N

H

T

I

I

F

-

T

-

P

P

F

T

C

A

A

A

R
x
K

Y

A

K

G

T

G

L

-

N

N

Y
|
Y

L

L

V

S

F

S

L

L

M

L

A

V

R

G

E

S

E

E

A

A

R

R

R

H

G

G

A

Y

Q

Q

E

E

A

G

L

I

L

A

V

L

D

D

R

V

E

N

E

G

H

Y

L

I

A

S

E
|
E

G

G

A

A

A
x
G

S
x
E

S

N

L

L

L

F

A

E

F

V

K

K

D

D

G

G

-

V

F

L

W

F

I

T

E

P

P

P

V

F

T

T

S

S

W

S

K

A

L
|
L

P

P

G
|
G

V
x
I

T
|
T

I

R

A

D

K

A

V

I

I

I

E

K

F

L

L

A

K

K

E

E

M

L

G

G

E

I

K

E

V

V

S

R

K

E

M

Q

R

V

F

L

S

S

V

R

E

E

D

S

L

L

R

Y

K

L

C

A

D

D

T

E

L

V

W

F

I

M

T

S

N
x
G

S
x
T

M
x
A

I

A

G

E

V

I

M

T

P

P

L

V

S

R

S

S

V

V

D

D

D

G

R

I

K

Q

L

V

P

G

D

E

L

G

K

R

A

C

G

G

active site: K156 (≠ R157)
binding 4-methyl valeric acid: Y92 (≠ I98), K156 (≠ R157), T254 (≠ S255), A255 (≠ M256)
binding pyridoxal-5'-phosphate: R56 (= R55), K156 (≠ R157), Y161 (= Y163), E190 (= E192), G193 (≠ A195), E194 (≠ S196), L214 (= L215), G216 (= G217), I217 (≠ V218), T218 (= T219), G253 (≠ N254), T254 (≠ S255)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
29% identity, 94% coverage: 8:276/287 of query aligns to 5:275/304 of 1i1lA

query
sites
1i1lA

V

I

W

W

W

F

N

N

G

G

R

E

F

M

E

V

H

R

V

W

N

E

A

D

V

A

R

K

I

V

S

H

P

V

L

M

D

S

R

H

G

A

L

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F

F

E

E

T

G

V

I

R

R

V

C

-

Y

-

D

-

S

E

H

K

K

G

G

R

P

A

V

L

V

F

F

L

R

E

H

-

R

E

E

H

H

L

M

N

Q

R
|
R

L

L

A

H

K

D

S

S

L

A

E

K

E

I

L

Y

R

R

I

F

N

-

P

P

N

V

L

S

P

Q

E

S

L

I

R

D

T

E

F

L

L

M

D

E

A

A

Y

-

E

-

T

-

A

-

H

-

I

-

V

C

H

R

D

D

L

V

I

I

R

R

R

K

N

N

G

N

L

L

E

T

D

S

R

A

I
x
Y

C

I

R

R

L

P

K

L

I

I

I

F

V

V

T

G

R

D

G

-

V

V

A

G

D

M

G

G

L

V

G

N

L

P

P

P

E

A

A

G

T

Y

A

S

P

T

T

D

V

V

I

I

M

I

M

A

V

A

F

F

P

P

Y

W

N

G

P

A

P

Y

-

L

N

G

P

A

E

E

E

A

Y

L

E

E

V

Q

G

G

-

I

-

D

-

A

-

M

-

V

-

S

W

W

K

N

A

R

I

A

T

A

L

P

R

N

H

T

I

I

F

-

T

-

P

P

F

T

C

A

A

A

R
x
K

Y

A

K

G

T

G

L

-

N

N

Y
|
Y

L

L

V

S

F

S

L

L

M

L

A

V

R

G

E

S

E

E

A

A

R

R

R

H

G

G

A

Y

Q

Q

E

E

A

G

L

I

L

A

V

L

D

D

R

V

E

N

E

G

H

Y

L

I

A

S

E
|
E

G

G

A

A

A
x
G

S

E

S

N

L

L

L

F

A

E

F

V

K

K

D

D

G

G

-

V

F

L

W

F

I

T

E

P

P

P

V

F

T

T

S

S

W

S

K

A

L

L

P

P

G
|
G

V
x
I

T
|
T

I

R

A

D

K

A

V

I

I

I

E

K

F

L

L

A

K

K

E

E

M

L

G

G

E

I

K

E

V

V

S

R

K

E

M

Q

R

V

F

L

S

S

V

R

E

E

D

S

L

L

R

Y

K

L

C

A

D

D

T

E

L

V

W

F

I

M

T

S

N

G

S
x
T

M
x
A

I

A

G

E

V

I

M

T

P

P

L

V

S

R

S

S

V

V

D

D

D

G

R

I

K

Q

L

V

P

G

D

E

L

G

K

R

A

C

G

G

active site: K156 (≠ R157)
binding 2-methylleucine: Y92 (≠ I98), K156 (≠ R157), T254 (≠ S255), A255 (≠ M256)
binding pyridoxal-5'-phosphate: R56 (= R55), K156 (≠ R157), Y161 (= Y163), E190 (= E192), G193 (≠ A195), G216 (= G217), I217 (≠ V218), T218 (= T219), T254 (≠ S255)

2y4rA Crystal structure of 4-amino-4-deoxychorismate lyase from pseudomonas aeruginosa (see paper)
32% identity, 92% coverage: 9:271/287 of query aligns to 3:252/270 of 2y4rA

query
sites
2y4rA

W

W

W

V

N

D

G

G

R

R

F

-

E

-

H

-

V

-

N

P

A

A

V

A

R

E

I

L

S

S

P

V

L

R

D

D

R

R

G

G

L

L

Q

A

Y

Y

G

G

D

D

G

G

L

L

F
|
F

E

E

T
|
T

V

L

R

A

V

V

E

R

K

A

G

G

R

T

A

P

L

R

F

L

L

L

E

E

E

R

H

H

L

L

N

A

R
|
R

L

L

A

E

K

E

S

G

L

C

E

R

L

L

R

A

I

I

N

P

P

L

N

D

L

T

P

A

E

A

L

L

R

R

T

Q

F

E

L

L

D

L

A

A

Y

F

E

C

T

A

A

A

H

-

I

-

V

-

H

-

D

-

L

-

I

-

R

-

N

-

G

-

L

L

E

G

D

D

R

G

I

V

C

A

R

-

L

-

K

K

I

L

I

I

V

V

T

T

R

R

G

G

V

E

A

G

-

L

D

R

G

G

L

Y

G

A

L

P

P

P

E

A

A

E

T

A

A

S

P

P

T

R

V

R

I

I

M

L

M

S

V

G

F

S

P

P

Y

-

N

R

P

P

P

A

N

Y

P

P

E

E

E

R

Y

H

E

-

V

-

G

-

W

W

K

Q

-

Q

-

G

-

V

-

R

-

L

-

F

A

A

I

C

T

R

L

T

R

R

H

L

I

A

F

E

T

Q

P

P

F

L

C

L

A

A

R

G

Y

L

K
|
K

T

H

L

L

N

N

Y

R

L

L

V

E

F

Q

L

V

M

L

A

A

R

R

E

A

E

E

A

W

R

S

R

D

R

A

G

G

A

H

Q

A

E

E

A

G

L

L

L

M

V

L

D

D

R

V

E

H

E

E

H

R

L

V

A

V

E
|
E

G

G

A

V

A

F

S
|
S

S

N

L

L

A

L

F

V

K

L

D

D

G

G

F

T

W

L

I

V

E

A

P

P

-

D

V

L

T

R

S

R

W

C

K

G

L
x
V

P

A

G
|
G

V
|
V

T

M

I

R

A

A

K

E

V

L

I

L

E

E

F

R

L

A

K

E

E

G

M

I

G

G

E

V

K

P

V

L

S

A

K

I

M

R

R

D

F

V

S

S

V

M

E

A

D

E

L

L

R

A

K

T

C

A

D

D

T

E

L

V

W

F

I

L

T

C

N
|
N

S
|
S

M

Q

I

F

G

G

V

I

M

W

P

P

L

V

S

R

S

A

V

L

D

D

D

E

R

H

K

V

L

W

P

P

active site: F26 (= F36), T28 (= T38), K139 (= K159), E172 (= E192), V196 (≠ L215)
binding pyridoxal-5'-phosphate: R45 (= R55), K139 (= K159), S176 (= S196), V196 (≠ L215), G198 (= G217), V199 (= V218), N235 (= N254), S236 (= S255)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
31% identity, 94% coverage: 7:276/287 of query aligns to 6:265/294 of 2ej2A

query
sites
2ej2A

I

L

V

I

W

W

W

M

N

N

G

G

R

A

F

F

E

V

H

P

V

Q

N

E

A

E

V

A

R

K

I

T

S

S

P

V

L

L

D

S

R

H

G

A

L

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

V

I

R

R

-

A

-

Y

-

E

V

T

E

A

K

K

G

G

R

P

A

A

L

I

F

F

-

R

L

L

E

K

E

E

H

H

L

V

N

K

R
|
R

L

F

A

Y

K

N

S

S

L

A

E

K

E

V

L

L

R

R

I

M

N

E

-

I

P

P

N

F

L

A

P

P

E

E

L

-

R

-

T

-

F

-

L

-

D

-

A

-

Y

-

E

E

T

L

A

E

H

E

I

A

V

I

H

K

D

E

L

V

I

V

R

R

N

N

G

G

L

Y

E

-

D

-

R

R

I

S

C

C

R
x
Y

L

I

K

R

I

P

I

L

V

A

T

W

R

M

G

G

V

-

A

A

D

K

G

A

L

L

G

G

L

-

P

-

E

-

A

-

T

-

A

-

P

-

T

-

V

-

I

-

M

-

V

V

F

N

P

P

Y

L

N

-

P

P

P

N

N

N

P

P

E

A

E

E

Y

V

E

M

V

V

G

A

-

A

-

W

-

E

W

W

K

G

A

A

I

R

T

L

L

I

R

T

H

S

I

S

F

W

T

A

P

R

F

F

C

P

A

A

R

N

Y

V

K

M

-

P

-

G

-

K

-

A

-
x
K

-

V

T

G

L

G

N

N

Y
|
Y

L

V

V

N

F

S

L

A

M

L

A

A

R

K

E

M

E

E

A

A

R

V

R

A

G

G

A

A

Q

D

E

E

A

A

L

L

V

L

D

D

R

E

E

E

E

G

H

Y

L

V

A

A

E
|
E

G

G

A

S

A
x
G

S
x
E

S

N

L

L

L

F

A

F

F

V

K

R

D

D

G

G

F

V

W

I

I

Y

E

A

P

L

V

E

T

H

S

S

W

V

K

N

L
|
L

P

E

G
|
G

V
x
I

T
|
T

I

R

A

D

K

S

V

V

I

I

E

R

F

I

L

A

K

K

E

D

M

L

G

G

E

Y

K

E

V

V

S

Q

K

V

M

V

R

R

F

A

S

T

V

R

E

D

D

Q

L

L

R

Y

K

M

C

A

D

D

T

E

L

V

W

F

I

M

T

T

N

G

S
x
T

M
x
A

I

A

G

E

V

V

M

T

P

P

L

V

S

S

S

M

V

I

D

D

D

W

R

R

K

P

L

I

P

G

D

K

L

G

K

T

A

A

G

G

active site: F35 (= F36), G37 (≠ T38), K147 (vs. gap), E181 (= E192), L204 (= L215)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R55), Y94 (≠ R100), Y152 (= Y163), E181 (= E192), G184 (≠ A195), E185 (≠ S196), L204 (= L215), G206 (= G217), I207 (≠ V218), T208 (= T219), T244 (≠ S255), A245 (≠ M256)

6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
28% identity, 94% coverage: 7:276/287 of query aligns to 29:295/321 of 6fteB

query
sites
6fteB

I

V

V

A

W

W

W

V

N

E

G

G

R

K

F

L

E

V

H

P

V

V

N

N

A

E

V

A

R

R

I

I

S

P

P

L

L

M

D

D

R

Q

G

G

L

F

Q

L

Y

H

G

S

D

D

G

L

L

T

F
x
Y

E

D

T

V

V

P

R

S

V

V

E

W

K

D

G

G

R

R

A

F

L

F

F

R

L

L

E

D

H

H

L

L

N

D

R
|
R

L

F

A

E

K

L

S

S

L

C

E

S

E

K

L

M

R

R

I

F

N

K

P

M

N

P

L

L

P

P

E

R

L

-

R

-

T

-

F

-

L

-

D

-

A

-

Y

Q

E

E

T

V

A

K

H

R

I

I

V

L

H

V

D

D

L

M

I

V

R

A

R

K

N

S

G

G

L

I

E

K

D

D

R

A

I

F

C

-

R

-

L

V

K

E

I

I

V

V

T

T

R

R

G

G

V

L

A

K

D

G

-

V

-

R

G

G

L

L

G

K

L

A

P

G

E

E

A

S

T

L

A

T

P

N

T

N

V

L

I

Y

M

M

M

W

V

I

F

Q

P

P

Y

Y

N

I

-

W

P

V

P

M

N

E

P

P

E

E

E

M

Y

Q

E

R

V

T

G

G

W

G

K

S

A

A

I

I

T

I

L

A

R

R

H

T

I

V

-

K

-

R

F

T

T

S

P

P

F

G

C

S

-

M

-

D

A

P

R

T

Y

V

K
|
K

T

N

L

L

N

Q

Y

W

L

G

V

D

F

L

L

T

M

R

A

G

R

M

E

L

E

E

A

A

R

Q

R

D

R

R