SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_093395033.1 NCBI__GCF_900114975.1:WP_093395033.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
50% identity, 77% coverage: 5:100/125 of query aligns to 10:104/200 of 6j2lA

query
sites
6j2lA

D

D

F

W

A

E

K

K

G

T

G

D

G

G

I

L

L

M

P

P

V

V

V

I

V

V

Q

Q

D

H

A

A

E

V

S

S

L

G

K

E

V

V

L

L

M

M

L

L

A

G

Y

Y

M

M

N

N

E

P

E

E

A

A

W

L

R

D

K

K

T

T

V

I

E

E

T

S

G

G

K

K

A

V

H

T

Y

F

W

F

S

S

R

R

S

T

R

K

G

Q

K

R

I

L

W

W

Q

I

K

K

G

G

E

E

T

T

S

S

G

G

H

N

F

F

Q

L

E

N

V

V

L

V

E

S

I

I

L

A

I

P

D
|
D

C

C

D
|
D

M

N

D
|
D

T

T

I

L

L

L

I

V

K

-

V

L

R

A

Q

N

H

P

G

I

G

G

G

P

A

T

C
|
C

H

H

E

K

G

G

Y

T

G

S

S

S

C
|
C

F

F

binding zinc ion: D79 (= D74), D81 (= D76), D83 (= D78), C96 (= C92), C103 (= C99)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
49% identity, 70% coverage: 5:92/125 of query aligns to 9:96/185 of 6j2lB

query
sites
6j2lB

D

D

F

W

A

E

K

K

G

T

G

D

G

G

I

L

L

M

P

P

V

V

V

I

V

V

Q

Q

D

H

A

A

E

V

S

S

L

G

K

E

V

V

L

L

M

M

L

L

A

G

Y

Y

M

M

N

N

E

P

E

E

A

A

W

L

R

D

K

K

T

T

V

I

E

E

T

S

G

G

K

K

A

V

H

T

Y

F

W

F

S

S

R

R

S

T

R

K

G

Q

K

R

I

L

W

W

Q

I

K

K

G

G

E

E

T

T

S

S

G

G

H

N

F

F

Q

L

E

N

V

V

L

V

E

S

I

I

L

A

I

P

D
|
D

C

C

D
|
D

M

N

D
|
D

T

T

I

L

L

L

I

V

K

L

V

A

R

N

Q

P

H

I

G

G

G

P

G

S

A

S

C

C

binding zinc ion: D78 (= D74), D80 (= D76), D82 (= D78)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
37% identity, 74% coverage: 9:101/125 of query aligns to 13:104/204 of 7bgnA

query
sites
7bgnA

G

N

G

K

G

G

I

L

L

A

P

V

V

A

V

I

V

A

Q

Q

D

N

A

V

E

D

S

T

L

G

K

A

V

I

L

L

M

M

L

Q

A

G

Y

F

M

A

N

N

E

R

E

E

A

A

W

V

R

A

K

T

T

T

V

I

E

S

T

S

G

R

K

K

A

A

H

T

Y

F

W

Y

S

S

R

R

S

S

R

R

G

S

K

S

I

L

W
|
W

Q

T

K
|
K

G
|
G

E

E

T
|
T

S
|
S

G

N

H

N

F

F

Q

I

E

N

V

V

L

H

E

D

I

V

L

F

I

L

D
|
D

C

C

D
|
D

M

R

D
|
D

T

S

I

I

L

-

I

I

K

Y

V

L

R

G

Q

K

H

P

G

D

G

G

G

P

A
x
T

C
|
C

H
|
H

E

T

G

G

Y

A

G

E

S

T

C
|
C

F

Y

Y

Y

binding adenosine monophosphate: W60 (= W56), K62 (= K58), G63 (= G59), T65 (= T61), S66 (= S62), T94 (≠ A91), H96 (= H93)
binding zinc ion: D78 (= D74), D80 (= D76), D82 (= D78), C95 (= C92), C102 (= C99)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
37% identity, 74% coverage: 9:101/125 of query aligns to 15:106/213 of 7bgmA

query
sites
7bgmA

G

N

G

K

G

G

I

L

L

A

P

V

V

A

V

I

V

A

Q

Q

D

N

A

V

E

D

S

T

L

G

K

A

V

I

L

L

M

M

L

Q

A

G

Y

F

M

A

N

N

E

R

E

E

A

A

W

V

R

A

K

T

T

T

V

I

E

S

T

S

G

R

K

K

A

A

H

T

Y

F

W

Y

S

S

R

R

S

S

R

R

G

S

K

S

I

L

W

W

Q

T

K

K

G

G

E

E

T

T

S

S

G

N

H

N

F

F

Q

I

E

N

V

V

L

H

E

D

I

V

L

F

I

L

D
|
D

C

C

D
|
D

M

R

D
|
D

T

S

I

I

L

-

I

I

K

Y

V

L

R

G

Q

K

H

P

G

D

G

G

G

P

A

T

C
|
C

H

H

E

T

G

G

Y

A

G

E

S

T

C
|
C

F

Y

Y

Y

binding zinc ion: D80 (= D74), D82 (= D76), D84 (= D78), C97 (= C92), C104 (= C99)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

Query Sequence

>WP_093395033.1 NCBI__GCF_900114975.1:WP_093395033.1
MEKPDFAKGGGILPVVVQDAESLKVLMLAYMNEEAWRKTVETGKAHYWSRSRGKIWQKGE
TSGHFQEVLEILIDCDMDTILIKVRQHGGGACHEGYGSCFYRRIDGDGWAVVEPRVFNPE
EVYGK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory