SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_093395105.1 NCBI__GCF_900114975.1:WP_093395105.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
50% identity, 99% coverage: 2:550/557 of query aligns to 7:560/562 of 6ovtA

query
sites
6ovtA

R

R

S

S

D

R

L

D

M

V

K

T

S

D

G

G

L

L

E

E

K

K

A

A

P

A

H

A

R

R

S

G

L

M

F

L

K

R

A

A

M

V

G

G

Y

M

T

D

D

D

E

E

E

D

L

F

Q

A

R

K

P

P

L

Q

I

I

G

G

I

V

V

A

N

S

S

S

A

W

N

N

E

E

I

I

I

T

P

P

G
x
C

H

N

I

L

H

S

L

L

D

D

R

R

I

L

A

A

E

N

A

A

V

V

K

K

A

E

G

G

V

V

R

F

M

S

A

A

G

G

G

G

T

Y

P

P

V

L

E

E

F

F

P

G

V

T

I

I

G

S

V

V

C

S

D
|
D

G
|
G

I

I

A

S

M

M

N

G

H

H

E

E

G

G

M

M

K

H

Y

F

S

S

L

L

A

V

S

S

R

R

E

E

L

V

I

I

A

A

D

D

S

S

V

V

E

E

L

V

M

V

A

M

K

Q

A

A

H

E

P

R

F

L

D

D

G

G

L

S

V

V

L

L

I

L

P

A

N

G

C
|
C

D
|
D

K
|
K

I

S

I

L

P

P

G

G

M

M

L

L

M

M

A

A

A

A

L

A

R

R

L

L

N

D

I

L

P

A

S

A

I

V

V

F

V

L

S

Y

G

A

G

G

P

S

M

I

L

L

A

P

G

G

R

R

L

A

-

K

-

L

-

S

-

D

G

G

S

S

Q

E

-

R

P

D

V

V

D

T

L

I

I

I

S

D

V

A

F

F

E

E

A

A

V

V

G

G

S

A

V

C

R

S

A

R

G

G

K

L

M

M

D

S

R

R

E

A

T

D

L

V

K

D

E

A

L

I

E

E

D

R

E

A

A

I

C

C

P

P

G

G

C

E

G

G

S
x
A

C
|
C

A

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

A

A

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

G

G

N

S

G

A

T

A

I

P

P

P

A

A

V

T

H

D

A

R

A

R

R

R

I

D

R

G

L

F

A

A

K

R

Y

R

A

S

G

G

M

Q

R

A

I

V

V

V

E

E

L

L

V

L

R

R

K

R

G

G

I

I

Y

T

P

A

R

R

D

D

I

I

V

L

V

T

K

K

E

E

A

A

F

F

E

E

N

N

A

A

I

I

A

A

V

V

D

V

M

M

A

A

L

F

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

V

L

P

L

A

A

I

I

A

A

H

H

E

E

A

A

G

N

I

V

S

A

I

L

D

S

L

L

N

Q

I

D

F

F

N

S

D

R

I

I

S

G

R

S

K

G

T

V

P

P

H

H

L

L

C

A

S

D

L

V

R

K

P

P

G

F

G

G

P

R

H

H

F

V

L

M

E

S

D

D

L

V

H

D

R

H

A

I

G

G

G

G

I

V

P

P

A

V

V

V

M

M

K

K

V

A

L

L

A

L

D

D

A

A

G

G

L

L

V

L

N

H

R

G

D

D

L

C

P

L

T

T

V

V

S

T

G

G

K

H

T

T

V

M

G

A

E

E

I

-

V

-

D

N

A

L

A

A

A

A

V

I

R

T

-

P

-

P

-

D

-

P

D

D

S

G

E

K

V

V

I

L

R

R

P

A

V

L

E

A

R

N

A

P

Y

I

H

H

A

P

E

S

G

G

G

G

I

I

A

T

V

I

L

L

Y

H

G

G

N

S

L

L

A

A

P

P

E

E

G

G

A

A

V

V

V

V

K

K

Q

T

S

A

A

G

V

F

A

D

P

S

E

D

M

V

M

F

K

E

R

-

T

-

G

G

R

T

A

A

R

R

V

V

F

F

D

D

S

G

E

E

Q

R

E

A

A

A

A

L

K

D

A

A

I

L

L

E

D

D

G

G

V

T

I

I

Q

T

P

V

G

G

E

D

V

A

V

V

V

V

I

I

R

R

Y

Y

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

R

E
|
E

M

M

L

L

S

A

P

I

T

T

A

G

A

A

I

I

V

K

G

G

R

A

G

G

L

L

D

G

K

K

D

D

V

V

A

L

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

T

R

T

G

G

A

L

A

C

I

V

G

G

H

H

V

I

S

A

P

P

E

E

A

A

A

V

A

D

G

G

G

G

T

P

I

I

A

A

L

L

V

L

K

R

D

N

G

G

D

D

E

R

I

I

L

R

I

L

D

D

I

V

P

A

G

G

K

R

R

V

L

L

E

D

L

V

L

L

V

A

D

D

E

P

G

A

T

E

L

F

E

A

K

S

R

R

R

Q

M

Q

E

D

W

F

T

S

P

P

P

P

P

P

P

P

R

R

I

Y

T

T

S

T

G

G

Y

V

L

L

A

S

R

K

Y

Y

A

V

A

K

M

L

V

V

T

S

S

S

G

A

A

A

R

V

G

G

A

A

V

V

binding fe2/s2 (inorganic) cluster: C51 (≠ G46), G84 (= G79), C124 (= C119), D125 (= D120), A200 (≠ S190), C201 (= C191)
binding magnesium ion: D83 (= D78), D125 (= D120), K126 (= K121), E452 (= E442)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 99% coverage: 2:550/557 of query aligns to 20:573/575 of P9WKJ5

query
sites
P9WKJ5

R

R

S

S

D

R

L

D

M

V

K

T

S

D

G

G

L

L

E

E

K

K

A

A

P

A

H

A

R

R

S

G

L

M

F

L

K

R

A

A

M

V

G

G

Y

M

T

D

D

D

E

E

E

D

L

F

Q

A

R

K

P

P

L

Q

I

I

G

G

I

V

V

A

N

S

S

S

A

W

N

N

E

E

I

I

I

T

P

P

G
x
C

H

N

I

L

H

S

L

L

D

D

R

R

I

L

A

A

E

N

A

A

V

V

K

K

A

E

G

G

V

V

R

F

M

S

A

A

G

G

G

G

T

Y

P

P

V

L

E

E

F

F

P

G

V

T

I

I

G

S

V

V

C

S

D
|
D

G

G

I

I

A

S

M

M

N

G

H

H

E

E

G

G

M

M

K

H

Y

F

S

S

L

L

A

V

S

S

R

R

E

E

L

V

I

I

A

A

D

D

S

S

V

V

E

E

L

V

M

V

A

M

K

Q

A

A

H

E

P

R

F

L

D

D

G

G

L

S

V

V

L

L

I

L

P

A

N

G

C
|
C

D
|
D

K
|
K

I

S

I

L

P

P

G

G

M

M

L

L

M

M

A

A

A

A

L

A

R

R

L

L

N

D

I

L

P

A

S

A

I

V

V

F

V

L

S

Y

G

A

G

G

P

S

M

I

L

L

A

P

G

G

R

R

L

A

-

K

-

L

-

S

-

D

G

G

S

S

Q

E

-

R

P

D

V

V

D

T

L

I

I

I

S

D

V

A

F

F

E

E

A

A

V

V

G

G

S

A

V

C

R

S

A

R

G

G

K

L

M

M

D

S

R

R

E

A

T

D

L

V

K

D

E

A

L

I

E

E

D

R

E

A

A

I

C

C

P

P

G

G

C

E

G

G

S

A

C
|
C

A

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

A

A

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

G

G

N

S

G

A

T

A

I

P

P

P

A

A

V

T

H

D

A

R

A

R

R

R

I

D

R

G

L

F

A

A

K

R

Y

R

A

S

G

G

M

Q

R

A

I

V

V

V

E

E

L

L

V

L

R

R

K

R

G

G

I

I

Y

T

P

A

R

R

D

D

I

I

V

L

V

T

K

K

E

E

A

A

F

F

E

E

N

N

A

A

I

I

A

A

V

V

D

V

M

M

A

A

L

F

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

V

L

P

L

A

A

I

I

A

A

H

H

E

E

A

A

G

N

I

V

S

A

I

L

D

S

L

L

N

Q

I

D

F

F

N

S

D

R

I

I

S

G

R

S

K

G

T

V

P

P

H

H

L

L

C

A

S

D

L

V

R

K

P

P

G

F

G

G

P

R

H

H

F

V

L

M

E

S

D

D

L

V

H

D

R

H

A

I

G

G

G

G

I

V

P

P

A

V

V

V

M

M

K

K

V

A

L

L

A

L

D

D

A

A

G

G

L

L

V

L

N

H

R

G

D

D

L

C

P

L

T

T

V

V

S

T

G

G

K

H

T

T

V

M

G

A

E

E

I

-

V

-

D

N

A

L

A

A

A

A

V

I

R

T

-

P

-

P

-

D

-

P

D

D

S

G

E

K

V

V

I

L

R

R

P

A

V

L

E

A

R

N

A

P

Y

I

H

H

A

P

E

S

G

G

G

G

I

I

A

T

V

I

L

L

Y

H

G

G

N

S

L

L

A

A

P

P

E

E

G

G

A

A

V

V

V

V

K

K

Q

T

S

A

A

G

V

F

A

D

P

S

E

D

M

V

M

F

K

E

R

-

T

-

G

G

R

T

A

A

R

R

V

V

F

F

D

D

S

G

E

E

Q

R

E

A

A

A

A

L

K

D

A

A

I

L

L

E

D

D

G

G

V

T

I

I

Q

T

P

V

G

G

E

D

V

A

V

V

V

V

I

I

R

R

Y

Y

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

R

E
|
E

M

M

L

L

S

A

P

I

T

T

A

G

A

A

I

I

V

K

G

G

R

A

G

G

L

L

D

G

K

K

D

D

V

V

A

L

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S
|
S

G

G

G

G

T

T

R

T

G

G

A

L

A

C

I

V

G

G

H

H

V

I

S

A

P

P

E

E

A

A

A

V

A

D

G

G

G

G

T

P

I

I

A

A

L

L

V

L

K

R

D

N

G

G

D

D

E

R

I

I

L

R

I

L

D

D

I

V

P

A

G

G

K

R

R

V

L

L

E

D

L

V

L

L

V

A

D

D

E

P

G

A

T

E

L

F

E

A

K

S

R

R

R

Q

M

Q

E

D

W

F

T

S

P

P

P

P

P

P

P

P

R

R

I

Y

T

T

S

T

G

G

Y

V

L

L

A

S

R

K

Y

Y

A

V

A

K

M

L

V

V

T

S

S

S

G

A

A

A

R

V

G

G

A

A

V

V

C64 (≠ G46) binding [2Fe-2S] cluster
D96 (= D78) binding Mg(2+)
C137 (= C119) binding [2Fe-2S] cluster
D138 (= D120) binding Mg(2+)
K139 (= K121) binding via carbamate group; modified: N6-carboxylysine
C214 (= C191) binding [2Fe-2S] cluster
E465 (= E442) binding Mg(2+)
S491 (= S468) mutation to A: Loss of catalytic activity.

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
41% identity, 96% coverage: 16:552/557 of query aligns to 70:607/608 of Q9LIR4

query
sites
Q9LIR4

R

Q

S

A

L

I

F

L

K

H

A

G

M

V

G

G

Y

L

T

S

D

D

E

D

E

D

L

L

Q

L

R

K

P

P

L

Q

I

I

G

G

I

I

V

S

N

S

S

V

A

W

N

Y

E

E

I

G

I

N

P

T

G
x
C

H

N

I

M

H

H

L

L

D

L

R

K

I

L

A

S

E

E

A

A

V

V

K

K

A

E

G

G

V

V

R

E

M

N

A

A

G

G

G

M

T

V

P

G

V

F

E

R

F

F

P

N

V

T

I

I

G

G

V

V

C

S

D

D

G

A

I

I

A

S

M

M

N

G

H

T

E

R

G

G

M

M

K

C

Y

F

S

S

L

L

A

Q

S

S

R

R

E

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

L

T

M

V

A

M

K

S

A

A

H

Q

P

W

F

Y

D

D

G

G

L

N

V

I

L

S

I

I

P

P

N

G

C
|
C

D
|
D

K

K

I

N

I

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

L

G

R

R

L

L

N

N

I

R

P

P

S

G

I

I

V

M

V

V

S

Y

G

G

G

G

P

T

M

I

L

K

A

P

G

G

R

H

L

F

G

Q

S

D

Q

K

P

T

V

Y

D

D

L

I

I

V

S

S

V

A

F

F

E

Q

A

S

V

Y

G

G

S

E

V

F

R

V

A

S

G

G

K

S

M

I

D

S

R

D

E

E

T

Q

L

R

K

K

E

T

L

V

E

L

D

H

E

H

A

S

C

C

P

P

G

G

C

A

G

G

S

A

C
|
C

A

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

A

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

T

S

I

I

P

P

A

A

V

E

H

D

A

P

A

L

R

K

I

L

R

D

L

E

A

C

K

R

Y

L

A

A

G

G

M

K

R

Y

I

L

V

L

E

E

L

L

V

L

R

K

K

M

G

D

I

L

Y

K

P

P

R

R

D

D

I

I

V

I

V

T

K

P

E

K

A

S

F

L

E

R

N

N

A

A

I

M

A

V

V

S

D

V

M

M

A

A

L

L

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

V

L

P

I

A

A

I

I

A

A

H

R

E

S

A

V

G

G

I

L

S

E

I

L

D

T

L

L

N

D

I

D

F

F

N

Q

D

K

I

V

S

S

R

D

K

A

T

V

P

P

H

F

L

L

C

A

S

D

L

L

R

K

P

P

G

S

G

G

P

K

H

Y

F

V

L

M

E

E

D

D

L

I

H

H

R

K

A

I

G

G

G

G

I

T

P

P

A

A

V

V

M

L

K

R

V

Y

L

L

A

L

D

E

A

L

G

G

L

L

V

M

N

D

R

G

D

D

L

C

P

M

T

T

V

V

S

T

G

G

K

Q

T

T

V

L

G

A

E

Q

I

N

V

L

D

E

A

N

-

V

-

P

A

S

A

L

V

T

R

E

D

G

S

Q

E

E

V

I

I

I

R

R

P

P

V

L

E

S

R

N

A

P

Y

I

H

K

A

E

E

T

G

G

G

H

I

I

A

Q

V

I

L

L

Y

R

G

G

N

D

L

L

A

A

P

P

E

D

G

G

A

S

V

V

V

A

K

K

Q

-

S

-

A

I

V

T

A

G

P

K

E

E

M

G

M

L

K

Y

R

F

T

S

G

G

R

P

A

A

R

L

V

V

F

F

D

E

S

G

E

E

Q

E

E

S

A

M

A

L

K

A

A

A

I

I

-

S

L

A

D

D

G

P

V

M

I

S

Q

F

P

K

G

G

E

T

V

V

V

V

V

V

I

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E
|
E

M

M

L

L

S

T

P

P

T

T

A

S

A

A

I

I

V

M

G

G

R

A

G

G

L

L

D

G

K

K

D

E

V

C

A

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

R

H

G

G

A

F

A

V

I

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

T

P

I

I

A

G

L

L

V

I

K

K

D

N

G

G

D

D

E

I

I

I

L

T

I

I

D

D

I

I

P

G

G

K

K

K

R

R

L

I

E

D

L

T

L

Q

V

V

D

S

E

P

G

E

T

E

L

M

E

N

K

D

R

R

R

R

M

K

E

K

W

W

T

T

P

A

P

P

P

A

P

Y

R

K

I

V

T

N

S

R

G

G

Y

V

L

L

A

Y

R

K

Y

Y

A

I

A

K

M

N

V

V

T

Q

S

S

G

A

A

S

R

D

G

G

A

C

V

V

L

T

E

D

C100 (≠ G46) binding [2Fe-2S] cluster
C173 (= C119) binding [2Fe-2S] cluster
D174 (= D120) binding Mg(2+)
C245 (= C191) binding [2Fe-2S] cluster
E497 (= E442) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
41% identity, 96% coverage: 16:552/557 of query aligns to 32:569/570 of 8hs0A

query
sites
8hs0A

R

Q

S

A

L

I

F

L

K

H

A

G

M

V

G

G

Y

L

T

S

D

D

E

D

E

D

L

L

Q

L

R

K

P

P

L

Q

I

I

G

G

I

I

V

S

N

S

S

V

A

W

N

Y

E

E

I

G

I

N

P

T

G
x
C

H

N

I

M

H

H

L

L

D

L

R

K

I

L

A

S

E

E

A

A

V

V

K

K

A

E

G

G

V

V

R

E

M

N

A

A

G

G

G

M

T

V

P

G

V

F

E

R

F

F

P

N

V

T

I

I

G

G

V

V

C

S

D
|
D

G
x
A

I

I

A

S

M

M

N

G

H

T

E

R

G

G

M

M

K

C

Y

F

S

S

L

L

A

Q

S

S

R

R

E

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

L

T

M

V

A

M

K

S

A

A

H

Q

P

W

F

Y

D

D

G

G

L

N

V

I

L

S

I

I

P

P

N

G

C
|
C

D
|
D

K
|
K

I

N

I

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

L

G

R

R

L

L

N

N

I

R

P

P

S

G

I

I

V

M

V

V

S

Y

G

G

G

G

P

T

M

I

L

K

A

P

G

G

R

H

L

F

G

Q

S

D

Q

K

P

T

V

Y

D

D

L

I

I

W

S

S

V

A

F

F

E

Q

A

S

V

Y

G

G

S

E

V

F

R

V

A

S

G

G

K

S

M

I

D

S

R

D

E

E

T

Q

L

R

K

K

E

T

L

V

E

L

D

H

E

H

A

S

C

C

P

P

G

G

C

A

G

G

S

A

C
|
C

A

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

A

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

T

S

I

I

P

P

A

A

V

E

H

D

A

P

A

L

R

K

I

L

R

D

L

E

A

C

K

R

Y

L

A

A

G

G

M

K

R

Y

I

L

V

L

E

E

L

L

V

L

R

K

K

M

G

D

I

L

Y

K

P

P

R

R

D

D

I

I

V

I

V

T

K

P

E

K

A

S

F

L

E

R

N

N

A

A

I

M

A

V

V

S

D

V

M

M

A

A

L

L

G

G

C

G

S

S

T

T

N
|
N

T

A

V

V

L

L

H
|
H

V

L

P

I

A

A

I

I

A

A

H

R

E

S

A

V

G

G

I

L

S

E

I

L

D

T

L

L

N

D

I

D

F

F

N

Q

D

K

I

V

S

S

R

D

K

A

T

V

P

P

H

F

L

L

C

A

S

D

L

L

R

K

P

P

G

S

G

G

P

K

H

Y

F

V

L

M

E

E

D

D

L

I

H

H

R

K

A

I

G

G

G

G

I

T

P

P

A

A

V

V

M

L

K

R

V

Y

L

L

A

L

D

E

A

L

G

G

L

L

V

M

N

D

R

G

D

D

L

C

P

M

T

T

V

V

S

T

G

G

K

Q

T

T

V

L

G

A

E

Q

I

N

V

L

D

E

A

N

-

V

-

P

A

S

A

L

V

T

R

E

D

G

S

Q

E

E

V

I

I

I

R

R

P

P

V

L

E

S

R

N

A

P

Y

I

H

K

A

E

E

T

G

G

G

H

I

I

A

Q

V

I

L

L

Y

R

G

G

N

D

L

L

A

A

P

P

E

D

G

G

A

S

V

V

V

A

K

K

Q

-

S

-

A

I

V

T

A

G

P

K

E

E

M

G

M

L

K

Y

R

F

T

S

G

G

R

P

A

A

R

L

V

V

F

F

D

E

S

G

E

E

Q

E

E

S

A

M

A

L

K

A

A

A

I

I

-

S

L

A

D

D

G

P

V

M

I

S

Q

F

P

K

G

G

E

T

V

V

V

V

V

V

I

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E

E

M

M

L

L

S

T

P

P

T

T

A

S

A

A

I

I

V

M

G

G

R

A

G

G

L

L

D

G

K

K

D

E

V

C

A

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

R

H

G

G

A

F

A

V

I

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

T

P

I

I

A

G

L

L

V

I

K

K

D

N

G

G

D

D

E

I

I

I

L

T

I

I

D

D

I

I

P

G

G

A

K

A

R

R

L

I

E

D

L

T

L

Q

V

V

D

S

E

P

G

E

T

E

L

M

E

N

K

D

R

R

R

R

M

K

E

K

W

W

T

T

P

A

P

P

P

A

P

Y

R

K

I

V

T

N

S

R

G

G

Y

V

L

L

A

Y

R

K

Y

Y

A

I

A

K

M

N

V

V

T

Q

S

S

G

A

A

S

R

D

G

G

A

C

V

V

L

T

E

D

binding fe2/s2 (inorganic) cluster: C62 (≠ G46), D94 (= D78), A95 (≠ G79), C135 (= C119), D136 (= D120), C207 (= C191)
binding magnesium ion: D136 (= D120), K137 (= K121), N285 (= N269), H289 (= H273)

8imuA Dihydroxyacid dehydratase (dhad) mutant-v497f
39% identity, 96% coverage: 16:552/557 of query aligns to 29:522/523 of 8imuA

query
sites
8imuA

R

Q

S

A

L

I

F

L

K

H

A

G

M

V

G

G

Y

L

T

S

D

D

E

D

E

D

L

L

Q

L

R

K

P

P

L

Q

I

I

G

G

I

I

V

S

N

S

S

V

A

W

N

Y

E

E

I

G

I
x
N

P

T

G
x
C

H

N

I

M

H

H

L

L

D

L

R

K

I

L

A

S

E

E

A

A

V

V

K

K

A

E

G

G

V

V

R

E

M

N

A

A

G

G

G

M

T

V

P

G

V

F

E

R

F

F

P

N

V

T

I

I

G

G

V

V

C

S

D

D

G

A

I

I

A

S

M

M

N

G

H

T

E

R

G

G

M

M

K

C

Y

F

S

S

L

L

A

Q

S

S

R

R

E

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

L

T

M

V

A

M

K

S

A

A

H

Q

P

W

F

Y

D

D

G

G

L

N

V

I

L

S

I

I

P

P

N

G

C
|
C

D
|
D

K

K

I

N

I

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

L

G

R

R

L

L

N

N

I

R

P

P

S

G

I

I

V

M

V

V

S

Y

G

G

G

G

P

-

M

-

L

-

A

-

G

-

R

-

L

-

G

-

S

-

Q

-

P

-

V

-

D

-

L

-

I

-

S

-

V

-

F

-

E

-

A

-

V

-

G

-

S

-

V

-

R

-

A

-

G

-

K

-

M

-

D

-

R

-

E

-

T

T

L

I

K

K

E

-

L

-

E

-

D

-

E

-

A

-

C

-

P

P

G

G

C

-

G

-

S

-

C

-

A

-

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

A

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

T

S

I

I

P

P

A

A

V

E

H

D

A

P

A

L

R

K

I

L

R

D

L

E

A

C

K

R

Y

L

A

A

G

G

M

K

R

Y

I

L

V

L

E

E

L

L

V

L

R

K

K

M

G

D

I

L

Y

K

P

P

R

R

D

D

I

I

V

I

V

T

K

P

E

K

A

S

F

L

E

R

N

N

A

A

I

M

A

V

V

S

D

V

M

M

A

A

L

L

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

V

L

P

I

A

A

I

I

A

A

H

R

E

S

A

V

G

G

I

L

S

E

I

L

D

T

L

L

N

D

I

D

F

F

N

Q

D

K

I

V

S

S

R

D

K

A

T

V

P

P

H

F

L

L

C

A

S

D

L

L

R

K

P

P

G

S

G

G

P

K

H

Y

F

V

L

M

E

E

D

D

L

I

H

H

R

K

A

I

G

G

G

G

I

T

P

P

A

A

V

V

M

L

K

R

V

Y

L

L

A

L

D

E

A

L

G

G

L

L

V

M

N

D

R

G

D

D

L

C

P

M

T

T

V

V

S

T

G

G

K

Q

T

T

V

L

G

A

E

Q

I

N

V

L

D

E

A

N

-

V

-

P

A

S

A

L

V

T

R

E

D

G

S

Q

E

E

V

I

I

I

R

R

P

P

V

L

E

S

R

N

A

P

Y

I

H

K

A

E

E

T

G

G

G

H

I

I

A

Q

V

I

L

L

Y

R

G

G

N

D

L

L

A

A

P

P

E

D

G

G

A

S

V

V

V

A

K

K

Q

-

S

-

A

I

V

T

A

G

P

K

E

E

M

G

M

L

K

Y

R

F

T

S

G

G

R

P

A

A

R

L

V

V

F

F

D

E

S

G

E

E

Q

E

E

S

A

M

A

L

K

A

A

A

I

I

-

S

L

A

D

D

G

P

V

M

I

S

Q

F

P

K

G

G

E

T

V

V

V

V

V

V

I

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E

E

M

M

L

L

S

T

P

P

T

T

A

S

A

A

I

I

V

M

G

G

R

A

G

G

L

L

D

G

K

K

D

E

V

C

A

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

R

H

G

G

A

F

A

V

I

F

G

G

H

H

V

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

T

P

I

I

A

G

L

L

V

I

K

K

D

N

G

G

D

D

E

I

I

I

L

T

I

I

D

D

I

I

P

G

G

A

K

A

R

R

L

I

E

D

L

T

L

Q

V

V

D

S

E

P

G

E

T

E

L

M

E

N

K

D

R

R

R

R

M

K

E

K

W

W

T

T

P

A

P

P

P

A

P

Y

R

K

I

V

T

N

S

R

G

G

Y

V

L

L

A

Y

R

K

Y

Y

A

I

A

K

M

N

V

V

T

Q

S

S

G

A

A

S

R

D

G

G

A

C

V

V

L

T

E

D

binding fe2/s2 (inorganic) cluster: N57 (≠ I44), C59 (≠ G46), C132 (= C119), D133 (= D120)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
40% identity, 97% coverage: 15:553/557 of query aligns to 27:565/579 of B5ZZ34

query
sites
B5ZZ34

H

H

R

R

S

G

L

W

F

L

K

K

A

N

M

Q

G

G

Y

Y

T

P

D

H

E

D

E

L

L

F

Q

D

-

G

R

R

P

P

L

V

I

I

G

G

I

I

V

L

N

N

S

T

A

W

N

S

E

D

I

M

I

T

P

P

G
x
C

H

N

I

G

H

H

L

L

D

R

R

E

I

L

A

A

E

E

A

K

V

V

K

K

A

A

G

G

V

V

R

W

M

E

A

A

G

G

G

G

T

F

P

P

V

L

E

E

F

V

P

P

V

V

I

F

G

S

V

A

C

S

D
x
E

G

N

I

-

A

T

M

F

N

R

H

P

E

T

G

A

M

M

K

M

Y

Y

S

-

L

-

A

-

S

-

R

R

E

N

L

L

I

A

A

A

D

L

S

A

V

V

E

E

L

E

M

A

A

I

K

R

A

G

H

Q

P

P

F

M

D

D

G

G

L

C

V

V

L

L

I

L

P

V

N

G

C
|
C

D
|
D

K

K

I

T

I

T

P

P

G

S

M

L

L

L

M

M

A

G

A

A

L

A

R

S

L

C

N

D

I

L

P

P

S

S

I

I

V

V

V

V

S

T

G

G

G

G

P

P

M

M

L

L

A

N

G

G

R

Y

L

F

G

R

S

G

Q

E

P

R

V

V

D

G

L

S

-

G

I

T

S

H

V

L

F

W

E

K

A

F

V

S

G

E

S

M

V

V

R

K

A

A

G

G

K

E

M

M

D

T

R

Q

E

A

T

E

L

F

K

L

E

E

L

A

E

E

D

A

E

S

A

M

C

S

P

R

G

S

C

S

G

G

S

T

C
|
C

A

N

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

A

E

E

A

A

L

L

G

G

L

M

A

A

L

L

P

S

G

G

N

N

G

A

T

A

I

I

P

P

A

G

V

V

H

D

A

S

A

R

R

R

I

K

R

V

L

M

A

A

K

Q

Y

L

A

T

G

G

M

R

R

R

I

I

V

V

E

Q

L

M

V

V

R

K

K

D

G

D

I

L

Y

K

P

P

R

S

D

E

I

I

V

M

V

T

K

K

E

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

V

T

D

N

M

A

A

A

L

I

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

I

H

H

V

L

P

L

A

A

I

I

A

A

H

G

E

R

A

V

G

G

I

I

S

D

I

L

D

S

L

L

N

D

I

D

F

W

N

D

D

R

I

C

S

G

R

R

K

D

T

V

P

P

H

T

L

I

C

V

S

N

L

L

R

M

P

P

G

S

G

G

P

K

H

Y

F

L

L

M

E

E

D

E

L

F

H

F

R

Y

A

A

G

G

G

G

I

L

P

P

A

V

V

V

M

L

K

K

V

R

L

L

A

G

D

E

A

A

G

G

L

L

V

L

N

H

R

K

D

D

L

A

P

L

T

T

V

V

S

S

G

G

K

E

T

T

V

V

G

W

E

D

I

E

V

V

D

K

A

D

A

V

A

V

V

N

R

W

D

N

S

E

E

D

V

V

I

I

R

L

P

P

V

A

E

E

R

K

A

A

Y

L

H

T

A

S

E

S

G

G

G

G

I

I

A

V

V

V

L

L

Y

R

G

G

N

N

L

L

A

A

P

P

E

K

G

G

A

A

V

V

V

L

K

K

Q

P

S

S

A

A

V

A

A

S

P

P

E

H

M

L

M

L

K

V

R

H

T

K

G

G

R

R

A

A

R

V

V

V

F

F

D

E

S

D

E

I

Q

D

E

D

A

Y

A

K

K

A

A

K

I

I

L

N

D

D

G

D

V

N

I

L

Q

D

P

I

G

D

E

E

-

N

-
x
C

V

I

V

M

V

V

I

M

R

K

Y

N

E
x
C

G

G

P

P

K

K

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

M

V

-

G

-

N

-

M

-

G

L

L

S

P

P

P

T

K

A

-

A

-

I

V

V

L

G

K

R

K

G

G

L

I

D

-

K

L

D

D

V

M

A

V

L

R

I

I

T

S

D

D

G

A

R

R

F

M

S
|
S

G

G

G

T

T

A

R

Y

G

G

A

T

A

V

I

V

G

L

H

H

V

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

T

P

I

L

A

A

L

V

V

V

K

K

D

N

G

G

D

D

E

M

I

I

L

E

I

L

D

D

I

V

P

P

G

N

K

R

R

R

L

L

E

H

L

L

L

D

V

I

D

S

E

D

G

E

T

E

L

L

E

A

K

R

R

R

R

L

M

A

E

E

W

W

T

Q

P

P

P

N

P

H

P

D

R

L

I

P

T

T

S

S

G

G

Y

Y

L

A

A

F

R

L

Y

H

A

Q

A

Q

M

H

V

V

T

E

S

G

G

A

A

D

R

T

G

G

A

A

V

D

L

L

E

D

L

F

C59 (≠ G46) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (≠ D78) binding Mg(2+)
C127 (= C119) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D120) binding Mg(2+)
C200 (= C191) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (vs. gap) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ E432) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E442) binding Mg(2+)
S480 (= S468) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
39% identity, 97% coverage: 15:553/557 of query aligns to 24:562/576 of 5j85A

query
sites
5j85A

H

H

R

R

S

G

L

W

F

L

K

K

A

N

M

Q

G

G

Y

Y

T

P

D

H

E

D

E

L

L

F

Q

D

-

G

R

R

P

P

L

V

I

I

G

G

I

I

V

L

N

N

S

T

A

W

N

S

E

D

I

M

I

T

P

P

G
x
C

H

N

I

G

H

H

L

L

D

R

R

E

I

L

A

A

E

E

A

K

V

V

K

K

A

A

G

G

V

V

R

W

M

E

A

A

G

G

G

G

T

F

P

P

V

L

E

E

F

V

P

P

V

V

I

F

G

S

V

A

C

S

D
x
E

G
x
N

I

-

A

T

M

F

N

R

H

P

E

T

G

A

M

M

K

M

Y

Y

S

-

L

-

A

-

S

-

R

R

E

N

L

L

I

A

A

A

D

L

S

A

V

V

E

E

L

E

M

A

A

I

K

R

A

G

H

Q

P

P

F

M

D

D

G

G

L

C

V

V

L

L

I

L

P

V

N

G

C
|
C

D
|
D

K
|
K

I

T

I

T

P

P

G

S

M

L

L

L

M

M

A

G

A

A

L

A

R

S

L

C

N

D

I

L

P

P

S

S

I

I

V

V

V

V

S

T

G

G

G

G

P

P

M

M

L

L

A

N

G

G

R

Y

L

F

G

R

S

G

Q

E

P

R

V

V

D

G

L

S

-

G

I

T

S

H

V

L

F

W

E

K

A

F

V

S

G

E

S

M

V

V

R

K

A

A

G

G

K

E

M

M

D

T

R

Q

E

A

T

E

L

F

K

L

E

E

L

A

E

E

D

A

E

S

A

M

C

S

P

R

G

S

C

S

G

G

S
x
T

C
|
C

A

N

G

T

M

M

F

G

T
|
T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

A

E

E

A

A

L

L

G

G

L

M

A

A

L

L

P

S

G

G

N

N

G

A

T

A

I

I

P

P

A

G

V

V

H

D

A

S

A

R

R

R

I

K

R

V

L

M

A

A

K

Q

Y

L

A

T

G

G

M

R

R

R

I

I

V

V

E

Q

L

M

V

V

R

K

K

D

G

D

I

L

Y

K

P

P

R

S

D

E

I

I

V

M

V

T

K

K

E

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

V

T

D

N

M

A

A

A

L

I

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

I

H

H

V

L

P

L

A

A

I

I

A

A

H

G

E

R

A

V

G

G

I

I

S

D

I

L

D

S

L

L

N

D

I

D

F

W

N

D

D

R

I

C

S

G

R

R

K

D

T

V

P

P

H

T

L

I

C

V

S

N

L

L

R

M

P

P

G

S

G

G

P

K

H

Y

F

L

L

M

E

E

D

E

L

F

H

F

R

Y

A

A

G

G

G

G

I

L

P

P

A

V

V

V

M

L

K

K

V

R

L

L

A

G

D

E

A

A

G

G

L

L

V

L

N

H

R

K

D

D

L

A

P

L

T

T

V

V

S

S

G

G

K

E

T

T

V

V

G

W

E

D

I

E

V

V

D

K

A

D

A

V

A

V

V

N

R

W

D

N

S

E

E

D

V

V

I

I

R

L

P

P

V

A

E

E

R

K

A

A

Y

L

H

T

A

S

E

S

G

G

G

G

I

I

A

V

V

V

L

L

Y

R

G

G

N

N

L

L

A

A

P

P

E

K

G

G

A

A

V

V

V

L

K

K

Q

P

S

S

A

A

V

A

A

S

P

P

E

H

M

L

M

L

K

V

R

H

T

K

G

G

R

R

A

A

R

V

V

V

F

F

D

E

S

D

E

I

Q

D

E

D

A

Y

A

K

K

A

A

K

I

I

L

N

D

D

G

D

V

N

I

L

Q

D

P

I

G

D

E

E

-

N

-

C

V

I

V

M

V

V

I

M

R

K

Y

N

E

C

G

G

P

P

K

K

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

M

V

-

G

-

N

-

M

-

G

L

L

S

P

P

P

T

K

A

-

A

-

I

V

V

L

G

K

R

K

G

G

L

I

D

-

K

L

D

D

V

M

A

V

L

R

I

I

T

S

D

D

G

A

R

R

F

M

S

A

G

G

G

T

T

A

R

Y

G

G

A

T

A

V

I

V

G

L

H

H

V

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

T

P

I

L

A

A

L

V

V

V

K

K

D

N

G

G

D

D

E

M

I

I

L

E

I

L

D

D

I

V

P

P

G

N

K

R

R

R

L

L

E

H

L

L

L

D

V

I

D

S

E

D

G

E

T

E

L

L

E

A

K

R

R

R

R

L

M

A

E

E

W

W

T

Q

P

P

P

N

P

H

P

D

R

L

I

P

T

T

S

S

G

G

Y

Y

L

A

A

F

R

L

Y

H

A

Q

A

Q

M

H

V

V

T

E

S

G

G

A

A

D

R

T

G

G

A

A

V

D

L

L

E

D

L

F

binding fe2/s2 (inorganic) cluster: C56 (≠ G46), N89 (≠ G79), C124 (= C119), D125 (= D120), T196 (≠ S190), C197 (= C191)
binding magnesium ion: E88 (≠ D78), D125 (= D120), K126 (= K121), T202 (= T196), E450 (= E442)

8ej0A Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mg
39% identity, 97% coverage: 15:556/557 of query aligns to 18:558/568 of 8ej0A

query
sites
8ej0A

H

Y

R

R

S

S

L

W

F

M

K

K

A

N

M

R

G

G

Y

I

T

P

D

H

E

D

E

Q

L

F

Q

D

-

G

R

R

P

P

L

V

I

I

G

G

I

I

V

C

N

N

S

T

A

F

N

S

E

E

I

L

I

T

P

P

G
x
C

H

N

I

S

H

H

L

F

D

R

R

T

I

L

A

A

E

E

A

Q

V

V

K

K

A

I

G

G

V

V

R

W

M

E

A

S

G

G

G

G

T

F

P

P

V

L

E

E

F

F

P

P

V

V

I

M

G

S

V

L

C

G

D
x
E

G

T

I

M

A

L

M

R

N

-

H

-

E

-

G

-

M

-

K

P

Y

T

S

A

L

M

A

L

S

F

R

R

E

N

L

L

I

A

A

S

D

M

S

D

V

V

E

E

L

E

M

S

A

I

K

R

A

G

H

N

P

P

F

L

D

D

G

G

L

V

V

V

L

L

I

L

P

M

N

G

C
|
C

D
|
D

K
|
K

I

T

I

T

P

P

G

S

M

L

L

M

M

M

A

G

A

A

L

A

R

S

L

C

N

D

I

L

P

P

S

T

I

I

V

G

V

V

S

S

G

G

G

G

P

P

M

M

L

L

A

S

G

G

R

K

L

F

-

R

G

G

S

R

Q

E

P

L

V

G

D

S

L

G

I

T

S

D

V

V

F
x
W

E

K

A

M

V

S

G

E

S

E

V

V

R

R

A

A

G

G

K

Q

M

M

D

S

R

Q

E

E

T

E

L

F

K

F

E

E

L

A

E

E

D

S

E

C

A

M

C

H

P

R

G

S

C

H

G

G

S

H

C
|
C

A

M

G

T

M

M

F

G

T
|
T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

V

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

G

G

N

N

G

A

T

A

I

I

P

P

A

A

V

V

H

D

A

A

A

R

R

R

I

N

R

L

L

L

A

A

K

R

Y

A

A

S

G

G

M

R

R

R

I

I

V

V

E

Q

L

M

V

V

R

K

K

D

G

D

I

L

Y

V

P

M

R

S

D

K

I

I

V

L

V

T

K

R

E

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

V

V

D

N

M

A

A

A

L

I

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

I

H

H

V

L

P

L

A

A

I

I

A

A

H

G

E

R

A

I

G

G

I

V

S

D

I

L

D

T

L

L

N

A

I

D

F

W

N

D

D

A

I

L

S

G

R

H

K

K

T

L

P

P

H

C

L

L

C

V

S

D

L

L

R

Q

P

P

G

S

G

G

P

T

H

H

F

L

L

M

E

E

D

D

L

F

H

Y

R

Y

A

A

G

G

G

G

I

V

P

P

A

A

V

V

M

I

K

R

V

E

L

L

A

G

D

D

A

-

G

-

L

V

V

I

N

A

R

R

D

D

L

A

P

L

T

T

V

V

S

N

G

G

K

Q

T

T

V

L

G

W

E

D

I

N

V

C

D

K

A

D

A

A

A

P

V

N

R

W

D

N

S

R

E

E

V

V

I

I

R

H

P

A

V

F

E

N

R

E

A

P

Y

F

H

K

A

T

E

E

G

A

G

G

I

I

A

A

V

V

L

L

Y

R

G

G

N

N

L

L

A

C

P

P

E

D

G

G

A

A

V

V

V

I

K

K

Q

P

S

S

A

A

V

A

A

T

P

P

E

A

M

L

M

L

K

K

R

H

T

K

G

G

R

R

A

A

R

V

V

V

F

F

D

E

S

N

E

S

Q

E

E

H

A

M

A

H

K

E

A

R

I

M

L

D

D

D

G

E

V

N

I

L

Q

D

P

V

G

D

E

E

-

N

-

C

V

V

V

L

V

V

I

L

R

K

Y

N

E

C

G

G

P

P

K

R

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

M

A

-

G

-

N

-

M

-

P

L

L

S

P

P

P

T

K

A

-

A

-

I

I

V

L

G

R

R

K

G

G

L

I

D

-

K

T

D

D

V

M

A

V

L

R

I

V

T

S

D

D

G

A

R

R

F

M

S
|
S

G
|
G

G

T

T

A

R

Y

G

G

A

T

A

V

I

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

T

P

I

L

A

A

L

L

V

V

K

Q

D

D

G

G

D

D

E

I

I

I

L

E

I

L

D

D

I

V

P

A

G

A

K

R

R

K

L

L

E

H

L

L

L

H

V

V

D

S

E

D

G

E

T

E

L

L

E

A

K

R

R

R

R

R

M

E

E

A

W

W

T

Q

P

A

P

P

P

P

P

A

R

P

I

M

T

A

S

R

G

G

Y

W

L

V

A

K

R

L

Y

Y

A

V

A

E

M

H

V

V

T

Q

S

Q

G

A

A

N

R

L

G

G

A

A

V

D

L

L

E

D

-

F

L

L

R

R

G

G

Q

K

binding bicarbonate ion: D119 (= D120), W161 (≠ F161), T196 (= T196), E442 (= E442), S469 (= S468), S469 (= S468), G470 (= G469)
binding fe2/s2 (inorganic) cluster: C50 (≠ G46), E82 (≠ D78), C118 (= C119), D119 (= D120), C191 (= C191)
binding magnesium ion: E82 (≠ D78), D119 (= D120), K120 (= K121), E442 (= E442)

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
39% identity, 97% coverage: 15:556/557 of query aligns to 19:559/569 of 8epzA

query
sites
8epzA

H

Y

R

R

S

S

L

W

F

M

K

K

A

N

M

R

G

G

Y

I

T

P

D

H

E

D

E

Q

L

F

Q

D

-

G

R

R

P

P

L

V

I

I

G

G

I

I

V

C

N

N

S

T

A

F

N

S

E

E

I

L

I

T

P

P

G
x
C

H

N

I

S

H

H

L

F

D

R

R

T

I

L

A

A

E

E

A

Q

V

V

K

K

A

I

G

G

V

V

R

W

M

E

A

S

G

G

G

G

T

F

P

P

V

L

E

E

F

F

P

P

V

V

I

M

G

S

V

L

C

G

D
x
E

G

T

I

M

A

L

M

R

N

-

H

-

E

-

G

-

M

-

K

P

Y

T

S

A

L

M

A

L

S

F

R

R

E

N

L

L

I

A

A

S

D

M

S

D

V

V

E

E

L

E

M

S

A

I

K

R

A

G

H

N

P

P

F

L

D

D

G

G

L

V

V

V

L

L

I

L

P

M

N

G

C
|
C

D
|
D

K
|
K

I

T

I

T

P

P

G

S

M

L

L

M

M

M

A

G

A

A

L

A

R

S

L

C

N

D

I

L

P

P

S

T

I

I

V

G

V

V

S

S

G

G

G

G

P

P

M

M

L

L

A

S

G

G

R

K

L

F

-

R

G

G

S

R

Q

E

P

L

V

G

D

S

L

G

I

T

S

D

V

V

F

W

E

K

A

M

V

S

G

E

S

E

V

V

R

R

A

A

G

G

K

Q

M

M

D

S

R

Q

E

E

T

E

L

F

K

F

E

E

L

A

E

E

D

S

E

C

A

M

C

H

P

R

G

S

C

H

G

G

S

H

C
|
C

A

M

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

V

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

G

G

N

N

G

A

T

A

I

I

P

P

A

A

V

V

H

D

A

A

A

R

R

R

I

N

R

L

L

L

A

A

K

R

Y

A

A

S

G

G

M

R

R

R

I

I

V

V

E

Q

L

M

V

V

R

K

K

D

G

D

I

L

Y

V

P

M

R

S

D

K

I

I

V

L

V

T

K

R

E

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

V

V

D

N

M

A

A

A

L

I

G

G

C

G

S

S

T

T

N

N

T

A

V

V

L

I

H

H

V

L

P

L

A

A

I

I

A

A

H

G

E

R

A

I

G

G

I

V

S

D

I

L

D

T

L

L

N

A

I

D

F

W

N

D

D

A

I

L

S

G

R

H

K

K

T

L

P

P

H

C

L

L

C

V