SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_093426890.1 NCBI__GCF_900112605.1:WP_093426890.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
68% identity, 100% coverage: 1:643/646 of query aligns to 1:642/648 of Q89WV5

query
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Q89WV5

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G263 (= G264) mutation to I: Loss of activity.
G266 (= G267) mutation to I: Great decrease in activity.
K269 (= K270) mutation to G: Great decrease in activity.
E414 (= E415) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
62% identity, 100% coverage: 1:643/646 of query aligns to 1:645/652 of P27550

query
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P27550

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K609 (= K607) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
62% identity, 100% coverage: 1:643/646 of query aligns to 1:645/652 of Q8ZKF6

query
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Q8ZKF6

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N

V

I

A

A

A

D

R

R

A

C

H

L

V

I

T

N

Q

P

A

E

E

Q

Y

Y

E

E

A

T

M

K

Y

Y

R

K

R

Q

S

S

V

I

D

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

D

-

R

K

F

I

L

L

D

D

W

W

F

I

E

T

K

P

W

Y

D

Q

T

K

T

V

L

K

D

N

W

T

S

S

F

F

E

A

-

P

G

G

D

N

V

V

H

S

V

I

E

K

W

W

F

Y

K

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

E

E

H

N

A

G

E

D

K

R

T

T

A

A

L

I

I

I

W

W

E

E

G

G

D

D

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D

P

T

D

S

Q

Q

D

S

Q

K

H

H

I

I

T

S

F

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

S

C

R

R

L

F

G

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

S

K

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

K

A

D

G

R

R

I

I

Q

I

D

D

A

S

E

S

A

S

S

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

G

A

G

G

R
|
R

K

S

V

I

G

P

L

L

K

K

A

K

N

N

T

V

D

D

K

D

A

A

V

L

D

K

Q

N

-

P

-

N

C

V

P

T

S

S

V

V

H

E

T

H

V

V

L

I

T

V

V

L

R

K

R

R

T

T

G

G

G

S

D

D

I

I

G

D

W

W

N

Q

D

E

S

G

R

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

V

I

E

E

A

K

A

A

S

S

A

P

D

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

I

T

T

T

T

F

W

K

Y

Y

T

V

F

F

D

D

L

Y

Q

H

P

P

D

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

T

A

T

T

S

T

V

L

V

M

F

F

E

E

G

G

V

V

P

P

S
x
N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

S

N

V

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

E

K

P

A

V

I

T

E

K

G

T

T

D

D

R

R

S

S

L

L

R

R

I

I

L

L

G

G

T

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

H

K

V

K

V

I

G

G

K

K

E

E

Q

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

H

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

E

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

V

Q

P

P

A

A

L

L

M

V

D

D

T

N

D

E

G

G

H

H

V

P

V

Q

E

E

G

G

E

A

G

T

E

E

G

G

A

N

L

L

V

V

I

I

T

T

R

D

P

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

I

L

Y

F

G

G

N

D

H

H

Q

E

R

R

F

F

V

E

E

Q

T

T

Y

Y

F

F

S

S

A

T

F

F

K

K

G

N

C

M

Y

Y

F

F

S

S

G

G

D

D

G

G

A

A

R

R

D

D

G

E

N

D

G

G

D

Y

Y

Y

W

W

I

I

T

T

G

G

R

R

M

V

D
|
D

D

D

V

V

L

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

L

A

H

H

D

P

A

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

V

N

K

H

D

G

A

E

E

E

P

P

S

S

D

P

A

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

L

W

V

V

R
|
R

S

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

A

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

N

G

E

D

L

T

D

S

S

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

A

G

V

V

D

E

E

K

L

L

I

L

N

E

R

K

R194 (= R194) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T309) binding CoA
N335 (≠ S333) binding CoA
A357 (= A355) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D515) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S521) binding CoA
G524 (= G522) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R524) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R582) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K607) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
62% identity, 99% coverage: 4:643/646 of query aligns to 1:638/640 of 5jrhA

query
sites
5jrhA

H

H

K

K

V

-

Y

H

P

A

V

I

P

P

E

A

S

N

V

I

A

A

A

D

R

R

A

C

H

L

V

I

T

N

Q

P

A

E

E

Q

Y

Y

E

E

A

T

M

K

Y

Y

R

K

R

Q

S

S

V

I

D

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

D

-

R

K

F

I

L

L

D

D

W

W

F

I

E

T

K

P

W

Y

D

Q

T

K

T

V

L

K

D

N

W

T

S

S

F

F

E

A

-

P

G

G

D

N

V

V

H

S

V

I

E

K

W

W

F

Y

K

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

E

E

H

N

A

G

E

D

K

R

T

T

A

A

L

I

I

I

W
|
W

E

E

G

G

D

D

E

D

P

T

D

S

Q

Q

D

S

Q

K

H

H

I

I

T

S

F

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

S

C

R

R

L

F

G

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

S

K

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E
|
E

A

A

V

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

K

A

D
x
G

R

R

I

I

Q

I

D

D

A

S

E

S

A

S

S

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

R

R

K

S

V

I

G

P

L

L

K

K

A

K

N

N

T

V

D

D

K

D

A

A

V

L

D

K

Q

N

-

P

-

N

C

V

P

T

S

S

V

V

H

E

T

H

V

V

L

I

T

V

V

L

R

K

R

R

T

T

G

G

G

S

D

D

I

I

G

D

W

W

N

Q

D

E

S

G

R

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

V

I

E

E

A

K

A

A

S

S

A

P

D

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

I

T

T

T

T

F

W

K

Y

Y

T

V

F

F

D

D

L

Y

Q

H

P

P

D

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

T

A

T

T

S

T

V

L

V

M

F

F

E

E

G

G

V

V

P

P

S

N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

S

N

V

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

E

K

P

A

V

I

T

E

K

G

T

T

D

D

R

R

S

S

L

L

R

R

I

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

H

K

V

K

V

I

G

G

K

K

E

E

Q

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

E

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

V

Q

P

P

A

A

L

L

M

V

D

D

T

N

D

E

G

G

H

H

V

P

V

Q

E

E

G

G

E

A

G

T

E

E

G

G

A

N

L

L

V

V

I

I

T

T

R

D

P

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

I

L

Y

F

G

G

N

D

H

H

Q

E

R

R

F

F

V

E

E

Q

T

T

Y

Y

F

F

S

S

A

T

F

F

K

K

G

N

C

M

Y

Y

F

F

S

S

G

G

D
|
D

G

G

A

A

R

R

D

D

G

E

N

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R

R

M

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

L

A

H
|
H

D

P

A

K

V
x
I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

V

N

K

H

D

G

A

E

E

E

P

P

S

S

D

P

A

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

L

W

V

V

R
|
R

S

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

A

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

N

G

E

D

L

-

D

-

S

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

A

G

V

V

D

E

E

K

L

L

I

L

N

E

R

K

active site: T260 (= T262), T412 (= T414), E413 (= E415), N517 (= N519), R522 (= R524), K605 (= K607)
binding (r,r)-2,3-butanediol: W93 (= W97), E140 (= E144), G169 (≠ D173), K266 (= K268), P267 (= P269)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G385), E384 (= E386), P385 (= P387), T408 (= T410), W409 (= W411), W410 (= W412), Q411 (= Q413), T412 (= T414), D496 (= D498), I508 (= I510), N517 (= N519), R522 (= R524)
binding coenzyme a: F159 (= F163), G160 (= G164), G161 (= G165), R187 (= R191), S519 (= S521), R580 (= R582), P585 (= P587)
binding magnesium ion: V533 (= V535), H535 (= H537), I538 (≠ V540)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
62% identity, 99% coverage: 4:643/646 of query aligns to 1:639/641 of 2p20A

query
sites
2p20A

H

H

K

K

V

-

Y

H

P

A

V

I

P

P

E

A

S

N

V

I

A

A

A

D

R

R

A

C

H

L

V

I

T

N

Q

P

A

E

E

Q

Y

Y

E

E

A

T

M

K

Y

Y

R

K

R

Q

S

S

V

I

D

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

D

-

R

K

F

I

L

L

D

D

W

W

F

I

E

T

K

P

W

Y

D

Q

T

K

T

V

L

K

D

N

W

T

S

S

F

F

E

A

-

P

G

G

D

N

V

V

H

S

V

I

E

K

W

W

F

Y

K

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

E

E

H

N

A

G

E

D

K

R

T

T

A

A

L

I

I

I

W

W

E

E

G

G

D

D

E

D

P

T

D

S

Q

Q

D

S

Q

K

H

H

I

I

T

S

F

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

S

C

R

R

L

F

G

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

S

K

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

K

A

D

G

R

R

I

I

Q

I

D

D

A

S

E

S

A

S

S

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

G

A

G

G

R

R

K

S

V

I

G

P

L

L

K

K

A

K

N

N

T

V

D

D

K

D

A

A

V

L

D

K

Q

N

-

P

-

N

C

V

P

T

S

S

V

V

H

E

T

H

V

V

L

I

T

V

V

L

R

K

R

R

T

T

G

G

G

S

D

D

I

I

G

D

W

W

N

Q

D

E

S

G

R

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

V

I

E

E

A

K

A

A

S

S

A

P

D

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

I

T

T

T

T

F

W

K

Y

Y

T

V

F

F

D

D

L

Y

Q

H

P

P

D

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

T

A

T

T

S

T

V

L

V

M

F

F

E

E

G

G

V

V

P

P

S

N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

S

N

V

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

E

K

P

A

V

I

T

E

K

G

T

T

D

D

R

R

S

S

L

L

R

R

I

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

H

K

V

K

V

I

G

G

K

K

E

E

Q

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

E

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

V

Q

P

P

A

A

L

L

M

V

D

D

T

N

D

E

G

G

H

H

V

P

V

Q

E

E

G

G

E

A

G

T

E

E

G

G

A

N

L

L

V

V

I

I

T

T

R

D

P

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

I

L

Y

F

G

G

N

D

H

H

Q

E

R

R

F

F

V

E

E

Q

T

T

Y

Y

F

F

S

S

A

T

F

F

K

K

G

N

C

M

Y

Y

F

F

S

S

G

G

D
|
D

G

G

A

A

R

R

D

D

G

E

N

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

M

V

D

D

V

V

L

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

L

A

H

H

D

P

A

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

V

N

K

H

D

G

A

E

E

E

P

P

S

S

D

P

A

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

L

W

V

V

R

A

S

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

A

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

N

G

E

-

L

-

D

D

S

T

L

S

G

N

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

A

G

V

V

D

E

E

K

L

L

I

L

N

E

R

K

active site: T260 (= T262), T412 (= T414), E413 (= E415), N517 (= N519), R522 (= R524), K605 (= K607)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G385), E384 (= E386), P385 (= P387), T408 (= T410), W409 (= W411), W410 (= W412), Q411 (= Q413), T412 (= T414), D496 (= D498), I508 (= I510), R511 (= R513), R522 (= R524)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
62% identity, 99% coverage: 7:643/646 of query aligns to 2:635/637 of 2p2fA

query
sites
2p2fA

Y

H

P

A

V

I

P

P

E

A

S

N

V

I

A

A

A

D

R

R

A

C

H

L

V

I

T

N

Q

P

A

E

E

Q

Y

Y

E

E

A

T

M

K

Y

Y

R

K

R

Q

S

S

V

I

D

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

D

-

R

K

F

I

L

L

D

D

W

W

F

I

E

T

K

P

W

Y

D

Q

T

K

T

V

L

K

D

N

W

T

S

S

F

F

E

A

-

P

G

G

D

N

V

V

H

S

V

I

E

K

W

W

F

Y

K

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

Q

E

E

H

N

A

G

E

D

K

R

T

T

A

A

L

I

I

I

W

W

E

E

G

G

D

D

E

D

P

T

D

S

Q

Q

D

S

Q

K

H

H

I

I

T

S

F

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

S

C

R

R

L

F

G

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

S

K

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

K

A

D

G

R

R

I

I

Q

I

D

D

A

S

E

S

A

S

S

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

R

R

K

S

V

I

G

P

L

L

K

K

A

K

N

N

T

V

D

D

K

D

A

A

V

L

D

K

Q

N

-

P

-

N

C

V

P

T

S

S

V

V

H

E

T

H

V

V

L

I

T

V

V

L

R

K

R

R

T

T

G

G

G

S

D

D

I

I

G

D

W

W

N

Q

D

E

S

G

R

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

V

I

E

E

A

K

A

A

S

S

A

P

D

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

I

T

T

T

T

F

W

K

Y

Y

T

V

F

F

D

D

L

Y

Q

H

P

P

D

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

A

G

T

A

T

T

S

T

V

L

V

M

F

F

E

E

G

G

V

V

P
|
P

S

N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

Q

Q

V

V

S

N

V

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

R

A

E

E

G

G

D

D

E

K

P

A

V

I

T

E

K

G

T

T

D

D

R

R

S

S

L

L

R

R

I

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

H

K

V

K

V

I

G

G

K

K

E

E

Q

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

E

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

I

V

V

Q

P

P

A

A

L

L

M

V

D

D

T

N

D

E

G

G

H

H

V

P

V

Q

E

E

G

G

E

A

G

T

E

E

G

G

A

N

L

L

V

V

I

I

T

T

R

D

P

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

I

L

Y

F

G

G

N

D

H

H

Q

E

R

R

F

F

V

E

E

Q

T

T

Y

Y

F

F

S

S

A

T

F

F

K

K

G

N

C

M

Y

Y

F

F

S

S

G

G

D
|
D

G

G

A

A

R

R

D

D

G

E

N

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

M

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

L

A

H

H

D

P

A

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

L

L

V

N

K

H

D

G

A

E

E

E

P

P

S

S

D

P

A

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

L

W

V

V

R
|
R

S

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

A

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

N

-

E

-

L

-

D

-

S

-

L

-

G

G

D

D

T

T

-

S

S

S

T

T

L

L

A

A

D

D

P

P

A

G

V

V

D

E

E

K

L

L

I

L

N

E

R

K

active site: T259 (= T262), T411 (= T414), E412 (= E415), N516 (= N519), R521 (= R524), K604 (= K607)
binding adenosine monophosphate: G382 (= G385), E383 (= E386), P384 (= P387), T407 (= T410), W408 (= W411), W409 (= W412), Q410 (= Q413), T411 (= T414), D495 (= D498), I507 (= I510), R510 (= R513), N516 (= N519), R521 (= R524)
binding coenzyme a: F158 (= F163), R186 (= R191), W304 (= W307), T306 (= T309), P329 (= P332), A352 (= A355), A355 (= A358), S518 (= S521), R579 (= R582), P584 (= P587)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
61% identity, 99% coverage: 4:643/646 of query aligns to 1:632/634 of 1pg3A

query
sites
1pg3A

H

H

K

K

V

-

Y

H

P

A

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I

P

P

E

A

S

N

V

I

A

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A

D

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A

C

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N

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P

A

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Y

Y

E

E

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Y

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D

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A

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A

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D

-

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K

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L

D

D

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W

F

I

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W

Y

D

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T

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T

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L

K

D

N

W

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S

F

F

E

A

-

P

G

G

D

N

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V

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S

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I

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K

W

W

F

Y

K

E

G

D

G

G

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T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

A

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E

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N

A

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T

A

A

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I

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D

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Y

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E

L

L

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N

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G

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A

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M

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M

M

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M

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L

A

A

C

C

A

A

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G

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A

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F

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G

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A

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A

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G

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R

G

A

G

G

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R

K

S

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I

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P

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L

K

K

A

K

N

N

T

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D

D

K

D

A

A

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D

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Q

N

-

P

-

N

C

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I

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K

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A

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D

N

A

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D

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P

L

L

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F

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T

S

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G

G

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T

T

G

G

K

K

P

P

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K

G

G

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V

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L

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H

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T

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G

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Y

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A

A

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Y

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D

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Y

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A

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G

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W

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T

G

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H

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Y

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L

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Y

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G

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P

L

L

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A

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C

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T

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W

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A

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A

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W

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D

D

K

K

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H

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Q

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Y

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A

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A
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A

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I

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A

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M

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A

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G

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E

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I

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N

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P

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A

A

W

W

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E

W

W

Y

Y

Y

W

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C

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T

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W

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T

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L

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A

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A

A

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Y

Y

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Y

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G

G

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D

G

G

A

A

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D

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D

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G

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W

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R

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D

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L

L

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N

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S

G

G

H

H

R
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R

M

L

G

G

T

T

A

A

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E

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I

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E

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S

A

A

L

L

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V

L

A

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H

D

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A

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V
x
I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

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Y

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V

I

T

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L

V

N

K

H

D

G

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A

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N

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R

S

K

E

E

I

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G

G

P
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P

I

L

A

A

S

T

P

P

D

D

A

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

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P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

N

G

E

D

L

-

D

-

S

-

L

-

G

-

D

-

T

-

S

-

T

-

L

L

A

A

D

D

P

P

A

G

V

V

D

E

E

K

L

L

I

L

N

E

R

K

active site: T260 (= T262), T412 (= T414), E413 (= E415), N517 (= N519), R522 (= R524), K605 (= K607)
binding coenzyme a: F159 (= F163), G160 (= G164), R187 (= R191), R190 (= R194), A301 (= A303), T307 (= T309), P330 (= P332), A356 (= A358), S519 (= S521), R580 (= R582), P585 (= P587)
binding magnesium ion: V533 (= V535), H535 (= H537), I538 (≠ V540)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G385), E384 (= E386), P385 (= P387), T408 (= T410), W409 (= W411), W410 (= W412), Q411 (= Q413), T412 (= T414), D496 (= D498), R511 (= R513), R522 (= R524)

Q9NR19 Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 from Homo sapiens (Human) (see paper)
55% identity, 98% coverage: 10:644/646 of query aligns to 32:697/701 of Q9NR19

query
sites
Q9NR19

P
|
P

E
x
P

S
x
E

V
|
V

A
x
S