SitesBLAST

P0AEN8 L-fucose mutarotase; D-ribose pyranase; Fucose 1-epimerase; Type-2 mutarotase; EC 5.1.3.29; EC 5.4.99.62 from Escherichia coli (strain K12) (see paper)
41% identity, 98% coverage: 1:137/140 of query aligns to 1:138/140 of P0AEN8

query
sites
P0AEN8

M

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H22 (= H22) active site, Proton donor; mutation to A: Affects slightly binding affinity toward L-fucose. Significantly reduced ribose pyranase activity.
D30 (= D30) binding substrate
D64 (= D64) active site; mutation to A: Affects slightly binding affinity toward L-fucose. Significantly reduced ribose pyranase activity.
M73 (≠ E75) binding substrate
Y111 (= Y110) active site; binding substrate; mutation to F: Reduces binding affinity toward L-fucose. Significantly reduced ribose pyranase activity.
Y129 (= Y128) binding substrate
N131 (= N130) binding substrate

4a34I Crystal structure of the fucose mutarotase in complex with l-fucose from streptococcus pneumoniae (see paper)
36% identity, 98% coverage: 2:138/140 of query aligns to 1:139/142 of 4a34I

query
sites
4a34I

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binding beta-L-fucopyranose: H21 (= H22), D29 (= D30), Y32 (= Y33), Y65 (≠ F66), Y129 (= Y128), V138 (= V137)

P04982 D-ribose pyranase; EC 5.4.99.62 from Escherichia coli (strain K12) (see paper)
26% identity, 93% coverage: 8:137/140 of query aligns to 6:137/139 of P04982

query
sites
P04982

L

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H20 (= H22) mutation to A: Loss of pyranase activity.
H106 (≠ R106) mutation to A: Retains one-third of the original activity.

1ogeA The structure of bacillus subtilis rbsd complexed with ribose 5- phosphate (see paper)
30% identity, 94% coverage: 8:138/140 of query aligns to 6:130/131 of 1ogeA

query
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1ogeA

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binding 5-O-phosphono-beta-D-ribofuranose: D28 (= D30), G30 (≠ N32), L31 (≠ Y33), H98 (≠ R106), Y120 (= Y128)

1ogdA The structure of bacillus subtilis rbsd complexed with d-ribose (see paper)
30% identity, 94% coverage: 8:138/140 of query aligns to 6:130/131 of 1ogdA

query
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1ogdA

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binding beta-D-ribopyranose: D28 (= D30), G30 (≠ N32), L31 (≠ Y33), H98 (≠ R106), Y120 (= Y128)

P36946 D-ribose pyranase; EC 5.4.99.62 from Bacillus subtilis (strain 168) (see paper)
30% identity, 94% coverage: 8:138/140 of query aligns to 6:130/131 of P36946

query
sites
P36946

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H98 (≠ R106) mutation to A: Reduced sugar-binding affinity.

Query Sequence

>WP_094505081.1 NCBI__GCF_002252445.1:WP_094505081.1
MLKNINPLLSGSLLEVLADMGHGDDLVIVDANYPAQASGVQVLDFPGISATDIAEAVLSL
LPLDDFVDKPAAVMEAPKETPAIFGEFEAVIEKAEGRKIAVDPIERFAFYDRASAAYAVI
RSGEKRLYGNIIFKKGVIRS

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory