SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_094505402.1 NCBI__GCF_002252445.1:WP_094505402.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
58% identity, 92% coverage: 11:339/358 of query aligns to 4:334/350 of Q88H32

query
sites
Q88H32

F

F

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F

R45 (= R52) binding L-ornithine
K69 (= K76) binding L-ornithine
T84 (= T91) binding NAD(+)
R112 (= R119) binding L-ornithine; binding NAD(+)
AQ 139:140 (= AQ 146:147) binding NAD(+)
D161 (= D168) binding NAD(+)
T202 (= T208) binding NAD(+)
VGGD 225:228 (= VGGD 231:234) binding NAD(+)
D228 (= D234) binding L-ornithine
K232 (= K238) binding NAD(+)
S293 (= S299) binding NAD(+)
V294 (= V300) binding L-ornithine
K331 (≠ R336) binding NAD(+)

1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
58% identity, 92% coverage: 11:339/358 of query aligns to 3:333/340 of 1x7dA

query
sites
1x7dA

F

F

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D

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active site: E55 (= E63), D227 (= D234)
binding nicotinamide-adenine-dinucleotide: T83 (= T91), R111 (= R119), T112 (= T120), G137 (= G145), A138 (= A146), Q139 (= Q147), D160 (= D168), T161 (≠ I169), V200 (= V207), T201 (= T208), A202 (= A209), I209 (= I216), V224 (= V231), G225 (= G232), D227 (= D234), K231 (= K238), S292 (= S299), V293 (= V300), G294 (= G301)
binding L-ornithine: R44 (= R52), V53 (= V61), E55 (= E63), M57 (= M65), K68 (= K76), V70 (= V78), N71 (= N79), G72 (= G80), R111 (= R119), D227 (= D234), V293 (= V300)

1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
58% identity, 92% coverage: 11:339/358 of query aligns to 3:333/341 of 1u7hA

query
sites
1u7hA

F

F

V

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Y

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F

active site: E55 (= E63), D227 (= D234)
binding nicotinamide-adenine-dinucleotide: T83 (= T91), R111 (= R119), T112 (= T120), G137 (= G145), A138 (= A146), Q139 (= Q147), D160 (= D168), T161 (≠ I169), V200 (= V207), T201 (= T208), A202 (= A209), I209 (= I216), V224 (= V231), G225 (= G232), D227 (= D234), K231 (= K238), S292 (= S299), V293 (= V300), G294 (= G301)

1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
32% identity, 80% coverage: 30:315/358 of query aligns to 17:306/320 of 1omoA

query
sites
1omoA

L

M

K

D

E

E

L

A

A

M

D

N

Y

A

V

V

E

E

D

A

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E

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x
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M

T

G

L

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Y

A

G

G

F

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K

Y

W

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V

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N

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A

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A

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K

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G

E

A

E

G

G

V

T

H

H

I

I

N

N

A

A

V

I

G
|
G

G

A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

H

D

G

V

D

E

I

I

L

L

R

K

S

A

D

K

I

I

F

V

V

V

E

D

Y

D

P

L

P

E

Q

Q

T

A

R

K

I

H

E

G

G

G

E

E

I

I

Q

N

Q

V

L

A

P

V

D

S

D

K

Y

G

P

V

V

I

N

G

-

V

-

E

-

D

-

V

-

H

-

A

E

T

L

I

W

G

E

E

V

V

I

I

T

A

G

G

K

L

T

K

E

D

G

G

R

R

K

E

D

S

A

D

R

E

Q

E

I

I

T

T

L

I

F

F

D

D

S
|
S

V
x
T

G
|
G

F

L

A

A

T

I

E

Q

D

D

F

V

S

A

A

V

L

A

R

K

Y

V

V

V

R

Y

D

E

active site: R52 (≠ E63), D219 (= D234)
binding nicotinamide-adenine-dinucleotide: T109 (= T120), G134 (= G145), T135 (≠ A146), Q136 (= Q147), Y156 (= Y167), D157 (= D168), V158 (≠ I169), R159 (≠ D170), T195 (≠ V207), P196 (≠ T208), G217 (= G232), D219 (= D234), K223 (= K238), S290 (= S299), T291 (≠ V300), G292 (= G301)

O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
32% identity, 80% coverage: 30:315/358 of query aligns to 17:306/322 of O28608

query
sites
O28608

L

M

K

D

E

E

L

A

A

M

D

N

Y

A

V

V

E

E

D

A

F

F

R

R

R

L

W

Y

E

A

-

L

-

G

S

K

F

A

D

Q

K

M

T

P

P

P

R

K

V

V

A

Y

S

L

H

E

S

F

D

E

E

K

G

G

V

D

I

L

E

R

L

A

M

M

P

P

T

A

S

H

D

L

G

M

T

G

L

Y

Y

A

G

G

F

L

K

K

Y

W

V

V

N

N

G

S

H

H

P

P

K

G

N

N

T

P

R

D

D

K

G

G

L

L

Q

P

T

T

V

V

T

M

A

A

F

L

G

M

V

I

L

L

S

N

D

S

V

P

G

E

N

T

G

G

Y

F

P

P

M

L

L

A

L

V

T

M

E

D

M

A

T

T

I

Y

L

T

T

T

A

S

L

L

R
|
R

T

T

A

G

A

A

T

A

S

G

A

G

V

I

A

A

K

K

H

Y

L

L

A

A

P

R

K

K

N

N

A

S

R

S

T

V

M

F

A

G

I

F

I

I

G

G

N

C

G

G

A
x
T

Q
|
Q

S

A

E

Y

F

F

Q

Q

A

L

L

E

A

A

F

L

K

R

A

R

I

V

L

F

G

D

I

I

D

G

K

E

L

V

R

K

L

A

Y

Y

D
|
D

I
x
V

D
x
R

R

E

S

K

A

A

S

A

E

K

K

K

C

F

A

V

R

S

N

Y

L

C

Q

E

G

D

T

R

G

G

I

I

E

S

I

A

T

S

I

V

C

-

K

Q

D

P

V

A

E

E

V

A

V

S

E

R

G

C

A

D

E

V

I

L

I

V

T

T

V

T

T

P

A

S

D

R

K

K

L

-

N

-

A

-

T

P

I

V

L

V

T

K

D

A

N

E

L

W

V

V

G

E

A

E

G

G

V

T

H

H

I

I

N

N

A

A

V

I

G
|
G

G
x
A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

H

D

G

V

D

E

I

I

L

L

R

K

S

A

D

K

I

I

F

V

V

V

E

D

Y

D

P

L

P

E

Q

Q

T

A

R

K

I

H

E

G

G

G

E

E

I

I

Q

N

Q

V

L

A

P

V

D

S

D

K

Y

G

P

V

V

I

N

G

-

V

-

E

-

D

-

V

-

H

-

A

E

T

L

I

W

G

E

E

V

V

I

I

T

A

G

G

K

L

T

K

E

D

G

G

R

R

K

E

D

S

A

D

R

E

Q

E

I

I

T

T

L

I

F

F

D

D

S
|
S

V

T

G

G

F

L

A

A

T

I

E

Q

D

D

F

V

S

A

A

V

L

A

R

K

Y

V

V

V

R

Y

D

E

R108 (= R119) binding NAD(+)
TQ 135:136 (≠ AQ 146:147) binding NAD(+)
DVR 157:159 (≠ DID 168:170) binding NAD(+)
GAD 217:219 (≠ GGD 232:234) binding NAD(+)
K223 (= K238) binding NAD(+)
S290 (= S299) binding NAD(+)

6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
31% identity, 84% coverage: 30:328/358 of query aligns to 15:325/325 of 6t3eB

query
sites
6t3eB

L

M

K

K

E

E

L

V

A

I

D

E

Y

S

V

V

E

E

E

R

D

A

F

F

R

L

R

E

-

L

W

Y

E

N

S

G

F

K

D

A

K

K

T

V

P

P

-

L

-
x
R

-

T

-

I

R

E

V

V

A

E

S

K

H

H

S

N

D

-

E

-

G

G

V

F

I

I

E

L

L

Y

M
|
M

P

P

T

S

-

Y

-

L

S

E

D

D

G

S

T

E

L

A

Y

L

G

A

F

V

K
|
K

Y

V

V

V

N

S

G

L

H

Y

P

P

K

E

N

N

T

T

R

K

D

K

G

G

L

L

Q

P

T
x
S

V

V

T

L

A

A

F

S

G

I

V

L

L

L

S

N

D

D

V

P

G

K

N

T

G

G

Y

A

P

P

M

L

L

A

L

L

T

M

E

E

M

G

T

T

I

F

L

I

T

T

A

A

L

M

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

G

V

V

A

A

A

T

K

K

H

Y

L

L

A

A

P

R

K

K

N

D

A

S

R

K

T

I

M

A

A

G

I

I

G

G

N

A

G
|
G

A
x
V

Q
|
Q

S

A

E

R

F

T

Q

Q

A

L

L

W

A

A

F

V

K

C

A

E

I

V

L

R

G

N

I

I

D

E

K

K

L

A

R

L

L

V

Y

Y

D
|
D

I
|
I

D

N

R

P

S

K

A

N

S

A

E

K

K

K

C

F

A

A

R

E

N

E

L

M

-

S

Q

K

G

K

T

L

G

G

I

I

E

E

I

I

T

K

I

T

C

V

K

E

D

S

V

A

E

R

E

E

V

A

V

T

E

E

G

K

A

S

E

D

I

I

I

L

T

I

T

V

V
x
A

T
|
T

A
x
T

D
x
A

K

R

L

-

N

-

A

E

T

P

I
x
V

L

V

T

K

D

G

N

G

L

W

V

I

G

R

A

E

G

G

V

T

H

H

I

I

N

N

A

S

V
|
V

G
|
G

G
x
W

D

V

C

G

P

R

G

D

K

A

T

R

E

E

L

L

H

D

G

S

D

E

I

T

L

V

R

R

R

K

S

S

D

K

I

L

F

V

V

V

E

D

Y

S

P

K

P

E

Q

G

T

V

R

L

I

N

E

E

-

S

G

G

E

D

I

I

-

I

-

P

-

M

-

K

Q

E

Q

G

L

V

P

I

D

D

D

E

Y

G

P

H

V

I

N

H

-

A

E

E

L

L

W

A

E

E

V

I

I

V

T

A

G

G

K

V

T

K

E

K

G

G

R

R

K

E

D

N

A

N

R

R

Q

E

I

I

T

T

L

L

F

F

D

K

S
|
S

V
|
V

G
|
G

F

L

A

A

T

I

E

E

D

D

F

A

S

I

A

T

L

A

R

K

Y

L

V

A

R

Y

D

E

K

K

L

A

K

L

D

E

T

H

G

G

F

V

Y

G

V

T

Q

N

L

V

D

E

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: S82 (≠ T91), T111 (= T120), G136 (= G145), V137 (≠ A146), Q138 (= Q147), D159 (= D168), I160 (= I169), A199 (≠ V207), T200 (= T208), T201 (≠ A209), A202 (≠ D210), V206 (≠ I216), V221 (= V231), G222 (= G232), W223 (≠ G233), S296 (= S299), V297 (= V300), G298 (= G301)
binding proline: R39 (vs. gap), M54 (= M65), K67 (= K76), R110 (= R119)

5gziA Cyclodeaminase_pa
32% identity, 79% coverage: 60:341/358 of query aligns to 64:347/354 of 5gziA

query
sites
5gziA

G

G

V

I

I

W

E

E

L

W

M

M

P

P

T

H

S

R

D

E

-

P

G

G

T

D

L

H

Y

I

G

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

D

F

G

G

L

L

Q

P

T
|
T

V

I

T

L

A

G

F

T

G

V

V

A

L

R

S

Y

D

D

V

D

G

T

N

T

G

G

Y

A

P

L

M

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T

T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

V

V

A

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

N

D

A

S

R

H

T

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

K

S

A

L

I

V

L

L

G

P

I

L

D

Q

K

R

L

A

R

L

L

V

Y

W

D
|
D

I
x
T

D

D

R

P

S

A

A
x
H

S

R

E

E

K

S

C

F

A

A

R

R

N

R

L

A

Q

A

G

F

T

T

G

G

I

V

E

S

I

V

T

E

I

I

C

A

K

E

D

P

V

A

E

R

E

I

V

A

E

E

G

-

A

A

E

D

I

V

I

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

L

V

-

G

N

Q

A

G

T

P

I

V

L

L

T

P

D

D

N

T

L

G

V

V

G

R

A

E

G

H

V

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D

D

C

L

P

V

G

G

K

K

T

T

E

E

L

L

H

P

G

L

D

G

I

L

L

L

R

E

R

R

S

A

D

F

I

V

F

T

V

A

E

D

Y

H

P

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

E

E

I

C

Q

Q

L

L

P

S

D

A

D

D

Y

R

P

L

V

G

N

P

E

Q

L

L

W

A

E

H

V

L

I

C

T

A

-

D

-

P

G

A

K

A

T

A

E

A

G

G

R

R

K

Q

D

D

A

-

R

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

T

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

V

F

R

L

D

E

K

A

L

A

K

A

D

E

T

R

G

D

F

L

Y

G

V

I

Q

R

L

V

D

G

L

I

L

E

A

H

D

H

P

P

D

G

E

D

P

A

R

L

D

D

L

P

Y

Y

G

A

M

L

binding nicotinamide-adenine-dinucleotide: Y85 (≠ G80), T97 (= T91), R125 (= R119), T126 (= T120), G151 (= G145), A152 (= A146), Q153 (= Q147), D174 (= D168), T175 (≠ I169), H179 (≠ A173), A212 (≠ V207), T213 (= T208), S214 (≠ A209), V215 (≠ D210), V237 (= V231), G238 (= G232), A239 (≠ G233), S305 (= S299), T306 (≠ V300), G307 (= G301)
binding (2S)-piperidine-2-carboxylic acid: K81 (= K76), R125 (= R119), A239 (≠ G233), T306 (≠ V300), G307 (= G301)

Sites not aligning to the query:

binding (2S)-piperidine-2-carboxylic acid: 53

5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
32% identity, 79% coverage: 60:341/358 of query aligns to 60:343/344 of 5yu4A

query
sites
5yu4A

G

G

V

I

I

W

E
|
E

L

W

M

M

P

P

T

H

S

R

D

E

-

P

G

G

T

D

L

H

Y

I

G

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

D

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

V

A

L

R

S

Y

D

D

V

D

G

T

N

T

G

G

Y

A

P

L

M

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T

T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

V

V

A

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

N

D

A

S

R

H

T

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

K

S

A

L

I

V

L

L

G

P

I

L

D

Q

K

R

L

A

R

L

L

V

Y

W

D
|
D

I
x
T

D

D

R

P

S

A

A
x
H

S

R

E

E

K

S

C

F

A

A

R

R

N

R

L

A

Q

A

G

F

T

T

G

G

I

V

E

S

I

V

T

E

I

I

C

A

K

E

D

P

V

A

E

R

E

I

V

A

E

E

G

-

A

A

E

D

I

V

I

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

L

V

-

G

N

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

N

T

L

G

V

V

G

R

A

E

G

H

V

L

H

H

I

I

N

N

A

A

V
|
V

G

G

G
x
A

D

D

C

L

P

V

G

G

K

K

T

T

E

E

L

L

H

P

G

L

D

G

I

L

L

L

R

E

R

R

S

A

D

F

I

V

F

T

V

A

E

D

Y

H

P

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

E

E

I

C

Q

Q

L

L

P

S

D

A

D

D

Y

R

P

L

V

G

N

P

E

Q

L

L

W

A

E

H

V

L

I

C

T

A

-

D

-

P

G

A

K

A

T

A

E

A

G

G

R

R

K

Q

D

D

A

-

R

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

T

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

V

F

R

L

D

E

K

A

L

A

K

A

D

E

T

R

G

D

F

L

Y

G

V

I

Q

R

L

V

D

G

L

I

L

E

A

H

D

H

P

P

D

G

E

D

P

A

R

L

D

D

L

P

Y

Y

G

A

M

L

binding 2,4-diaminobutyric acid: E63 (= E63), K77 (= K76), R121 (= R119), T302 (≠ V300), G303 (= G301)
binding nicotinamide-adenine-dinucleotide: Y81 (≠ G80), T93 (= T91), I94 (≠ V92), R121 (= R119), T122 (= T120), G147 (= G145), A148 (= A146), Q149 (= Q147), D170 (= D168), T171 (≠ I169), H175 (≠ A173), A208 (≠ V207), T209 (= T208), S210 (≠ A209), V211 (≠ D210), V218 (≠ I216), V233 (= V231), A235 (≠ G233), S301 (= S299), T302 (≠ V300), G303 (= G301)

5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
32% identity, 79% coverage: 60:341/358 of query aligns to 60:343/344 of 5yu3A

query
sites
5yu3A

G

G

V

I

I

W

E

E

L

W

M
|
M

P

P

T

H

S

R

D

E

-

P

G

G

T

D

L

H

Y

I

G

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

D

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

V

A

L

R

S

Y

D

D

V

D

G

T

N

T

G

G

Y

A

P

L

M

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T

T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

V

V

A

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

N

D

A

S

R

H

T

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

K

S

A

L

I

V

L

L

G

P

I

L

D

Q

K

R

L

A

R

L

L

V

Y

W

D
|
D

I
x
T

D

D

R

P

S

A

A

H

S

R

E

E

K

S

C

F

A

A

R

R

N

R

L

A

Q

A

G

F

T

T

G

G

I

V

E

S

I

V

T

E

I

I

C

A

K

E

D

P

V

A

E

R

E

I

V

A

E

E

G

-

A

A

E

D

I

V

I

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

L

V

-

G

N

Q

A

G

T

P

I

V

L

L

T

P

D

D

N

T

L

G

V

V

G

R

A

E

G

H

V

L

H

H

I

I

N

N

A

A

V
|
V

G

G

G
x
A

D

D

C

L

P

V

G

G

K

K

T

T

E

E

L

L

H

P

G

L

D

G

I

L

L

L

R

E

R

R

S

A

D

F

I

V

F

T

V

A

E

D

Y

H

P

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

E

E

I

C

Q

Q

L

L

P

S

D

A

D

D

Y

R

P

L

V

G

N

P

E

Q

L

L

W

A

E

H

V

L

I

C

T

A

-

D

-

P

G

A

K

A

T

A

E

A

G

G

R

R

K

Q

D

D

A

-

R

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

T

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

V

F

R

L

D

E

K

A

L

A

K

A

D

E

T

R

G

D

F

L

Y

G

V

I

Q

R

L

V

D

G

L

I

L

E

A

H

D

H

P

P

D

G

E

D

P

A

R

L

D

D

L

P

Y

Y

G

A

M

L

binding nicotinamide-adenine-dinucleotide: Y81 (≠ G80), T93 (= T91), I94 (≠ V92), T122 (= T120), G147 (= G145), A148 (= A146), Q149 (= Q147), D170 (= D168), T171 (≠ I169), A208 (≠ V207), T209 (= T208), S210 (≠ A209), V211 (≠ D210), V233 (= V231), A235 (≠ G233), S301 (= S299), T302 (≠ V300), G303 (= G301)
binding proline: M65 (= M65), K77 (= K76), R121 (= R119)

5gzlA Cyclodeaminase_pa
32% identity, 79% coverage: 60:341/358 of query aligns to 64:347/357 of 5gzlA

query
sites
5gzlA

G

G

V
x
I

I

W

E
|
E

L

W

M

M

P

P

T

H

S

R

D

E

-

P

G

G

T

D

L

H

Y

I

G

T

F

L

K

K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

D

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

V

A

L

R

S

Y

D

D

V

D

G

T

N

T

G

G

Y

A

P

L

M

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T

T

A

A

L

L

R

R

T
|
T

A

G

A

A

T

A

S

S

A

A

V

V

A

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

N

D

A

S

R

H

T

T

M

L

A

G

I

L

I

I

G

G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

K

S

A

L

I

V

L

L

G

P

I

L

D

Q

K

R

L

A

R

L

L

V

Y

W

D
|
D

I
x
T

D

D

R

P

S

A

A
x
H

S

R

E

E

K

S

C

F

A

A

R

R

N

R

L

A

Q

A

G

F

T

T

G

G

I

V

E

S

I

V

T

E

I

I

C

A

K

E

D

P

V

A

E

R

E

I

V

A

E

E

G

-

A

A

E

D

I

V

I

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D

V

K

A

L

V

-

G

N

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

N

T

L

G

V

V

G

R

A

E

G

H

V

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

H

P

G

L

D

G

I

L

L

L

R

E

R

R

S

A

D

F

I

V

F

T

V

A

E

D

Y

H

P

P

P

E

Q

Q

T

A

R

L

I
x
R

E
|
E

G

G

E

E

I

C

Q

Q

L

L

P

S

D

A

D

D

Y

R

P

L

V

G

N

P

E

Q

L

L

W

A

E

H

V

L

I

C

T

A

-

D

-

P

G

A

K

A

T

A

E

A

G

G

R

R

K

Q

D

D

A

-

R

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

T

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

V

F

R

L

D

E

K

A

L

A

K

A

D

E

T

R

G

D

F

L

Y

G

V

I

Q

R

L

V

D

G

L

I

L

E

A

H

D

H

P

P

D

G

E

D

P

A

R

L

D

D

L

P

Y

Y

G

A

M

L

binding lysine: I65 (≠ V61), E67 (= E63), D240 (= D234), R267 (≠ I261), E268 (= E262)
binding nicotinamide-adenine-dinucleotide: Y85 (≠ G80), T97 (= T91), I98 (≠ V92), T126 (= T120), G151 (= G145), A152 (= A146), Q153 (= Q147), D174 (= D168), T175 (≠ I169), H179 (≠ A173), A212 (≠ V207), T213 (= T208), S214 (≠ A209), V222 (≠ I216), V237 (= V231), G238 (= G232), A239 (≠ G233), D240 (= D234), K244 (= K238), S305 (= S299), T306 (≠ V300), G307 (= G301)

Sites not aligning to the query:

binding lysine: 55, 57

4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
32% identity, 61% coverage: 97:315/358 of query aligns to 87:298/303 of 4bvaA

query
sites
4bvaA

V

L

L

L

S

F

D

D

V

P

G

S

N

N

G

G

Y

S

P

L

M

L

L

A

L

V

T

M

E

D

M

G

T

N

I

V

L

I

T
|
T

A

A

L

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

V

I

A

A

A

T

K

K

H

L

L

L

A

K

P

P

K

P

N

G

A

S

R

D

T

V

M

L

A

C

I

I

I

L

G

G

N

A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

K

T

A

E

I

Q

L

F

G

S

I

F

D

K

K

E

L

V

R

R

L

M

Y

W

D
x
N

I
x
R

D
x
T

R

R

S

E

A
x
N

S

A

E

E

K

K

C

F

A

A

R

S

N

T

L

V

Q

Q

G

G

T

-

G

-

I

-

E

D

I

V

T

R

I

V

C

C

K

S

D

S

V

V

E

Q

E

E

V

A

V

V

E

T

G

G

A

A

E

D

I

V

I

I

T

I

T

T

V
|
V

T
|
T

A

-

D

-

K
x
M

L
x
A

N

T

A

E

T

P

I

I

L

L

T

F

D

G

N

E

L

W

V

V

G

K

A

P

G

G

V

A

H

H

I

I

N

N

A

A

V
|
V

G

G

G

A

D
x
S

C
x
R

P

P

G

D

K
x
W

T

R

E

E

L

L

H

D

G

D

D

E

I

L

L

M

R

R

R

Q

S

A

D

V

I

L

F

Y

V

V

E

D

Y

S

P

R

P

E

Q

A

T

A

R

L

I

K

E
|
E

G

S

E

G

I

D

Q

V

Q

L

L

L

P

S

D

G

D

A

Y

D

P

I

V

F

N

A

E

E

L

L

W

G

E

E

V

V

I

I

T

S

G

G

K

A

T

K

E

P

G

A

R

H

K

-

D

-

A

C

R

E

Q

K

I

T

T

T

L

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

T

V

E

E

D

D

F

L

S

V

A

A

L

A

R

K

Y

L

V

V

R

Y

D

D

active site: S219 (≠ D234)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T116), R109 (= R119), T110 (= T120), G135 (= G145), V136 (≠ A146), Q137 (= Q147), N158 (≠ D168), R159 (≠ I169), T160 (≠ D170), N163 (≠ A173), V194 (= V207), T195 (= T208), M196 (≠ K211), A197 (≠ L212), V216 (= V231), S282 (= S299), L283 (≠ V300), G284 (= G301)
binding 3,5,3'triiodothyronine: S219 (≠ D234), R220 (≠ C235), W223 (≠ K238), E247 (= E262)

Sites not aligning to the query:

active site: 59
binding 3,5,3'triiodothyronine: 46, 57, 59, 76, 78

4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
32% identity, 61% coverage: 97:316/358 of query aligns to 88:300/303 of 4bv9A

query
sites
4bv9A

V

L

L

L

S

F

D

D

V

P

G

S

N

N

G

G

Y

S

P

L

M

L

L

A

L

V

T

M

E

D

M

G

T

N

I

V

L

I

T

T

A

A

L

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

V

I

A

A

A

T

K

K

H

L

L

L

A

K

P

P

K

P

N

G

A

S

R

D

T

V

M

L

A

C

I

I

I

L

G
|
G

N

A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

K

T

A

E

I

Q

L

F

G

S

I

F

D

K

K

E

L

V

R

R

L

M

Y

W

D
x
N

I
x
R

D
x
T

R

R

S

E

A

N

S

A

E

E

K

K

C

F

A

A

R

S

N

T

L

V

Q

Q

G

G

T

-

G

-

I

-

E

D

I

V

T

R

I

V

C

C

K

S

D

S

V

V

E

Q

E

E

V

A

V

V

E

T

G

G

A

A

E

D

I

V

I

I

T

I

T

T

V
|
V

T
|
T

A

-

D

-

K
x
M

L
x
A

N

T

A

E

T

P

I

I

L

L

T

F

D

G

N

E

L

W

V

V

G

K

A

P

G

G

V

A

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G

A

D
x
S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

H

D

G

D

D

E

I

L

L

M

R

R

R

Q

S

A

D

V

I

L

F

Y

V

V

E

D

Y

S

P

R

P

E

Q

A

T

A

R

L

I

K

E

E

G

S

E

G

I

D

Q

V

Q

L

L

L

P

S

D

G

D

A

Y

D

P

I

V

F

N

A

E

E

L

L

W

G

E

E

V

V

I

I

T

S

G

G

K

A

T

K

E

P

G

A

R

H

K

-

D

-

A

C

R

E

Q

K

I

T

T

T

L

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

T

V

E

E

D

D

F

L

S

V

A

A

L

A

R

K

Y

L

V

V

R

Y

D

D

K

S

active site: S220 (≠ D234)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T111 (= T120), G134 (= G143), G136 (= G145), V137 (≠ A146), Q138 (= Q147), N159 (≠ D168), R160 (≠ I169), T161 (≠ D170), V195 (= V207), T196 (= T208), M197 (≠ K211), A198 (≠ L212), V217 (= V231), G218 (= G232), S283 (= S299), L284 (≠ V300), G285 (= G301)
binding pyruvic acid: R110 (= R119)

Sites not aligning to the query:

active site: 58
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 82, 83
binding pyruvic acid: 45, 60, 73

Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
29% identity, 74% coverage: 50:315/358 of query aligns to 74:308/314 of Q14894

query
sites
Q14894

T

T

P

K

R

L

V

V

A

T

S

F

H

Y

S

E

D

D

E

R

G

G

V

I

I

T

E

S

L

V

M

V

P

P

T

S

S

H

D

Q

G

A

T

T

L

V

Y

L

G

L

F

F

K

E

Y

P

V

S

N

N

G

G

H

T

P

L

K

L

N

A

T

V

R

M

D

D

G

G

L

-

Q

-

T

-

V

-

T

-

A

-

F

-

G

-

V

-

L

-

S

-

D

-

V

-

G

-

N

-

G

-

Y

-

P

-

M

-

L

-

T

-

E

-

M

-

T

N

I

V

L

I

T

T

A

A

L

K

R

R

T

T

A

A

T

V

S

S

A

A

V

I

A

A

A

T

K

K

H

F

L

L

A

K

P

P

K

P

N

S

A

S

R

E

T

V

M

L

A

C

I

I

I

L

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S
x
A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

K

T

A

E

I

Q

L

F

G

S

I

F

D

K

K

E

L

V

R

R

L

I

Y

W

D
x
N

I
x
R

D

T

R

K

S

E

A

N

S

A

E

E

K

K

C

F

A

A

R

D

N

T

L

V

Q

Q

G

G

T

-

G

-

I

-

E

E

I

V

T

R

I

V

C

C

K

S

D

S

V

V

E

Q

E

E

V

A

V

V

E

A

G

G

A

A

E

D

I

V

I

I

T

I

T

T

V

V

T

T

A

L

D

A

K

-

L

-

N

T

A

E

T

P

I

I

L

L

T

F

D

G

N

E

L

W

V

V

G

K

A

P

G

G

V

A

H

H

I

I

N

N

A

A

V

V

G

G

G

A

D

S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

H

D

G

D

D

E

I

L

L

M

R

K

R

E

S

A

D

V

I

L

F

Y

V

V

E

D

Y

S

P

Q

P

E

Q

A

T

A

R

L

I

K

E

E

G

S

E

G

I

D

Q

V

Q

L

L

L

P

S

D

G

D

A

Y

E

P

I

V

F

N

A

E

E

L

L

W

G

E

E

V

V

I

I

T

K

G

G

K

V

T

K

E

P

G

A

R

H

K

-

D

-

A

C

R

E

Q

K

I

T

T

T

L

V

F

F

D

K

S

S

V

L

G

G

F

M

A

A

T

V

E

E

D

D

F

T

S

V

A

A

L

A

R

K

Y

L

V

I

R

Y

D

D

GAGVQA 143:148 (≠ GNGAQS 143:148) binding NADP(+)
N168 (≠ D168) binding NADP(+)
R169 (≠ I169) binding NADP(+)

2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
29% identity, 74% coverage: 50:315/358 of query aligns to 73:307/312 of 2i99A

query
sites
2i99A

T

T

P

K

R

L

V

V

A

T

S

F

H

Y

S

E

D
|
D

E

R

G

G

V

I

I

T

E

S

L

V

M

V

P

P

T
x
S

S
x
H

D

Q

G

A

T

T

L

V

Y

L

G

L

F

F

K

E

Y

P

V

S

N

N

G

G

H

T

P

L

K

L

N

A

T

V

R

M

D

D

G

G

L

-

Q

-

T

-

V

-

T

-

A

-

F

-

G

-

V

-

L

-

S

-

D

-

V

-

G

-

N

-

G

-

Y

-

P

-

M

-

L

-

T

-

E

-

M

-

T

N

I

V

L

I

T

T

A

A

L

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

V

I

A

A

A

T

K

K

H

F

L

L

A

K

P

P

K

P

N

S

A

S

R

E

T

V

M

L

A

C

I

I

I

L

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

K

T

A

E

I

Q

L

F

G

S

I

F

D

K

K

E

L

V

R

R

L

I

Y

W

D
x
N

I
x
R

D
x
T

R

K

S

E

A

N

S

A

E

E

K

K

C

F

A

A

R

D

N

T

L

V

Q

Q

G

G

T

-

G

-

I

-

E

E

I

V

T

R

I

V

C

C

K

S

D

S

V

V

E

Q

E

E

V

A

V

V

E

A

G

G

A

A

E

D

I

V

I

I

T

I

T

T

V
|
V

T
|
T

A
x
L

D
x
A

K

-

L

-

N

T

A

E

T

P

I

I

L

L

T

F

D

G

N

E

L

W

V

V

G

K

A

P

G

G

V

A

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G

A

D
x
S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

H

D

G

D

D

E

I

L

L

M

R

K

R

E

S

A

D

V

I

L

F

Y

V

V

E

D

Y

S

P

Q

P

E

Q

A

T

A

R

L

I

K

E

E

G

S

E

G

I

D

Q

V

Q

L

L

L

P

S

D

G

D

A

Y

E

P

I

V

F

N

A

E

E

L

L

W

G

E

E

V

V

I

I

T

K

G

G

K

V

T

K

E

P

G

A

R

H

K

-

D

-

A

C

R

E

Q

K

I

T

T

T

L

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

T

V

E

E

D

D

F

T

S

V

A

A

L

A

R

K

Y

L

V

I

R

Y

D

D

active site: S228 (≠ D234)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: D81 (= D58), S90 (≠ T67), H91 (≠ S68), R118 (= R119), T119 (= T120), G142 (= G143), A143 (≠ N144), G144 (= G145), V145 (≠ A146), Q146 (= Q147), N167 (≠ D168), R168 (≠ I169), T169 (≠ D170), V203 (= V207), T204 (= T208), L205 (≠ A209), A206 (≠ D210), V225 (= V231), G226 (= G232), S291 (= S299), L292 (≠ V300), G293 (= G301)

Sites not aligning to the query:

active site: 59

6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
30% identity, 72% coverage: 72:328/358 of query aligns to 65:322/322 of 6rqaB

query
sites
6rqaB

L

V

Y

L

G

G

F

L

K

K

Y

A

V

G

N

G

G

Y

H

W

P

P

K
x
N

N

N

T

Q

R

K

D

H

G

N

L

L

Q

I

T

N

V

H

T

Q

A

S

F

T

G

V

V

F

L

L

S

F

D

D

V

P

G

D

N

T

G

G

Y

R

P

V

M

S

L

A

T

V

E

G

M

G

T

N

I

L

L

L

T

T

A

A

L

L

R

R

T
|
T

A

A

T

A

S

S

A

A

V

V

A

S

A

I

K

K

H

Y

L

L

A

A

P

P

K

K

N

G

A

A

R

K

T

V

M

L

A

G

I

M

I

I

G

G

N

A

G
|
G

A

H

Q
|
Q

S

S

E

A

F

F

Q

Q

A

M

L

R

A

A

F

A

K

A

A

N

I

V

L

H

G

R

I

F

D

E

K

K

L

V

R

-

L

-

Y

-

D

-

I

I

D

G

R

W

S

N

A
x
P

S
x
H

E

P

K

E

C

M

A

L

R

S

N

R

L

L

Q

A

G

D

T

T

G

A

I

A

E

E

I

L

T

G

I

L

C

P

K

F

D

E

V

A

E

V

E

E

V

L