SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_094506262.1 NCBI__GCF_002252445.1:WP_094506262.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
33% identity, 80% coverage: 59:292/292 of query aligns to 50:296/296 of P68183

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P68183

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E190 (= E186) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A188) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G192) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P205) mutation to A: No effect.

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
27% identity, 97% coverage: 10:292/292 of query aligns to 3:281/281 of P94530

query
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P94530

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D180 (= D191) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
29% identity, 33% coverage: 157:253/292 of query aligns to 357:456/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: N361 (≠ Y161), G365 (≠ N165), Y368 (≠ F168), S418 (≠ A218), N422 (≠ S222)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 310

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
29% identity, 33% coverage: 157:253/292 of query aligns to 372:471/514 of P02916

query
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P02916

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L372 (= L157) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ Y161) mutation N->K,H: No growth on maltose.
G380 (≠ N165) mutation G->C,S: No growth on maltose.
E401 (= E186) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A188) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G192) mutation G->A,P: No effect.
P420 (= P205) mutation to A: No effect.

Sites not aligning to the query:

334 L→W: Ability to transport lactose in a saturable manner.

Query Sequence

>WP_094506262.1 NCBI__GCF_002252445.1:WP_094506262.1
MASTTLSNDQHRLLRRVKKVAYLIGLFLAMTIICLPGLWIVLSSLRPPVEIMAKPPVWIP
QEITLDAYYAMFSGAGGGGIPVWDYFRNSLIISVTSTIIALVVGVSGGYAFARFRFWGKS
TTFLGLMLTRSVPGIALSLPLFMLYSRIGIIDTHFGLIITYVALNVPFTIWLIDGFFRQV
PKDLAEAAQIDGCTRWQAFWQVEFPLAGPGIATAGIFAFLTSWNEYALASQLTRSINSKT
LPVGLLDYTAEFTIDWRGMCALAVVMIIPALTLTFIVQKHLVAGLTFGAVKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory