SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_094506690.1 NCBI__GCF_002252445.1:WP_094506690.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
57% identity, 96% coverage: 5:303/312 of query aligns to 3:301/306 of P0DX97

query
sites
P0DX97

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M10 (= M12) Important for substrate specificity; mutation to N: 2.5-fold decrease in Vmax for deoxyribokinase activity and 70-fold increase in KM for deoxyribose. 2-fold increase in KM for ribose.

6wjzA Crystal structure of human ribokinase in complex with ampcp
38% identity, 92% coverage: 7:294/312 of query aligns to 7:298/315 of 6wjzA

query
sites
6wjzA

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binding phosphomethylphosphonic acid adenosyl ester: N186 (= N186), T222 (= T222), G224 (= G224), A225 (≠ G225), G227 (= G227), T243 (≠ P241), V246 (= V244), A254 (= A252), G255 (= G253), N282 (≠ A278), A285 (= A281), A286 (= A282), V289 (≠ I285)

2fv7A Crystal structure of human ribokinase
38% identity, 92% coverage: 7:294/312 of query aligns to 6:297/308 of 2fv7A

query
sites
2fv7A

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active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
binding adenosine-5'-diphosphate: N185 (= N186), T221 (= T222), G223 (= G224), G226 (= G227), T242 (≠ P241), V245 (= V244), A253 (= A252), G254 (= G253), N281 (≠ A278), A284 (= A281), A285 (= A282), V288 (≠ I285)

5c3yA Structure of human ribokinase crystallized with amppnp
38% identity, 92% coverage: 7:294/312 of query aligns to 6:297/306 of 5c3yA

query
sites
5c3yA

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active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
binding amp phosphoramidate: T221 (= T222), G223 (= G224), V245 (= V244), T250 (= T249), G254 (= G253), N281 (≠ A278), A284 (= A281), A285 (= A282)

6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
38% identity, 92% coverage: 7:294/312 of query aligns to 7:298/311 of 6wk0B

query
sites
6wk0B

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K
|
K

G

G

A

A

N
|
N

Q

Q

A

C

V

V

A

Q

A

A

A

A

R

R

L

L

G

G

A

A

D

M

V

T

M

S

M

M

V

V

T

C

R

K

V

V

G

G

D

K

D

D

V

S

F

F

A

G

D

N

N

D

T

Y

I

I

R

E

N

N

L

L

N

K

N

Q

F

N

G

D

V

I

D

S

T

T

R

E

H

F

V

T

V

Y

K

Q

I

T

S

K

G

D

K

A

S

A

S

T

G

G

V

T

A
|
A

P

S

I

I

F

I

V

V

E

N

P

N

S

E

G

G

E

Q

N

N

S

I

I

I

L

V

I

I

V

V

K

A

G

G

A

A

N

N

A

L

D

L

L

L

L

N

P

T

A

E

E

D

V

L

D

R

K

A

A

A

S

A

E

N

D

V

L

I

K

S

T

R

C

A

G

K

L

V

I

M

L

V

M

C

Q

Q

M

L

E
|
E

V

I

P

T

V

P

E

A

T

T

V

S

Y

L

H

E

T

A

I

L

S

T

F

M

A

A

A

R

E

R

N

S

G

G

I

V

E

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

A

I

A

A

D

D

L

L

D

D

P

P

E

Q

R

F

I

Y

R

T

Q

L

V

S

T

D

F

V

L

F

V

C

P

C

N
|
N

E

E

S

S

E

E

L

A

A

E

L

I

L

L

S

T

G

G

L

L

P

T

T

V

D

G

T

S

N

A

D

A

E

D

I

A

V

G

I

E

A

A

A

A

R

L

S

V

L

L

I

L

E

K

R

R

G

G

I

C

R

Q

T

V

V

V

I

I

V

I

T
|
T

L

L

G
|
G

G
x
A

R

E

G
|
G

A

C

R

V

M

V

I

L

T

S

A

Q

N

T

-

E

-

P

E

E

I

P

V

K

N

H

I

I

P

P

P
x
T

V

E

K

K

V
|
V

T

K

P

A

K

V

D

D

T

T

T

T

G

G

A
|
A

G
|
G

D
|
D

A

S

F

F

I

V

G

G

S

A

F

L

A

A

R

F

F

Y

-

L

-

A

Y

Y

A

Y

E

P

T

N

G

L

E

S

V

L

E

E

A

D

S

M

L

L

K

N

K

R

A

S

A
x
N

L

F

Y

I

A
|
A

A
|
A

H

V

S

S

I
x
V

T

Q

R

A

P

A

G

G

T

T

Q

Q

K

S

A

S

Y

Y

binding phosphomethylphosphonic acid adenylate ester: N186 (= N186), T222 (= T222), G224 (= G224), A225 (≠ G225), G227 (= G227), T243 (≠ P241), V246 (= V244), A254 (= A252), G255 (= G253), N282 (≠ A278), A285 (= A281), A286 (= A282), V289 (≠ I285)
binding alpha-D-ribofuranose: D14 (= D14), G40 (= G40), K41 (= K41), N44 (= N44), A96 (= A96), E141 (= E141), D256 (= D254)

5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
38% identity, 92% coverage: 7:294/312 of query aligns to 7:298/317 of 5c41A

query
sites
5c41A

V

V

V

V

G

G

S

S

N

C

M

M

V

T

D

D

L

L

I

V

T

S

Y

L

V

T

N

S

R

R

M

L

P

P

G

K

P

T

G

G

E

E

T

T

L

I

E

H

A

G

P

H

T

K

F

F

E

F

I

I

G

G

C

F

G

G

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

A

A

R

R

L

L

G

G

A

A

D

M

V

T

M

S

M

M

V

V

T

C

R

K

V

V

G

G

D

K

D

D

V

S

F

F

A

G

D

N

N

D

T

Y

I

I

R

E

N

N

L

L

N

K

N

Q

F

N

G

D

V

I

D

S

T

T

R

E

H

F

V

T

V

Y

K

Q

I

T

S

K

G

D

K

A

S

A

S

T

G

G

V

T

A

A

P

S

I

I

F

I

V

V

E

N

P

N

S

E

G

G

E

Q

N

N

S

I

I

I

L

V

I

I

V

V

K

A

G

G

A

A

N

N

A

L

D

L

L

L

L

N

P

T

A

E

E

D

V

L

D

R

K

A

A

A

S

A

E

N

D

V

L

I

K

S

T

R

C

A

G

K

L

V

I

M

L

V

M

C

Q

Q

M

L

E

E

V

I

P

T

V

P

E

A

T

T

V

S

Y

L

H

E

T

A

I

L

S

T

F

M

A

A

A

R

E

R

N

S

G

G

I

V

E

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

A

I

A

A

D

D

L

L

D

D

P

P

E

Q

R

F

I

Y

R

T

Q

L

V

S

T

D

F

V

L

F

V

C

P

C

N
|
N

E

E

S

S

E

E

L

A

A

E

L

I

L

L

S

T

G

G

L

L

P

T

T

V

D

G

T

S

N

A

D

A

E

D

I

A

V

G

I

E

A

A

A

A

R

L

S

V

L

L

I

L

E

K

R

R

G

G

I

C

R

Q

T

V

V

V

I

I

V

I

T
|
T

L

L

G
|
G

G
x
A

R

E

G
|
G

A

C

R

V

M

V

I

L

T

S

A

Q

N

T

-

E

-

P

E

E

I

P

V

K

N

H

I

I

P

P

P

T

V

E

K

K

V
|
V

T

K

P

A

K

V

D

D

T

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

A

S

F

F

I

V

G

G

S

A

F

L

A

A

R

F

F

Y

-

L

-

A

Y

Y

A

Y

E

P

T

N

G

L

E

S

V

L

E

E

A

D

S

M

L

L

K

N

K

R

A

S

A
x
N

L

F

Y

I

A
|
A

A
|
A

H

V

S

S

I

V

T

Q

R

A

P

A

G

G

T

T

Q

Q

K

S

A

S

Y

Y

active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N186), T222 (= T222), G224 (= G224), A225 (≠ G225), G227 (= G227), V246 (= V244), G255 (= G253), N282 (≠ A278), A285 (= A281), A286 (= A282)

5byfA Crystal structure of human ribokinase in complex with amp
38% identity, 92% coverage: 9:294/312 of query aligns to 10:299/313 of 5byfA

query
sites
5byfA

G

G

S

S

N

C

M

M

V

T

D

D

L

L

I

V

T

S

Y

L

V

T

N

S

R

R

M

L

P

P

G

K

P

T

G

G

E

E

T

T

L

I

E

H

A

G

P

H

T

K

F

F

E

F

I

I

G

G

C

F

G

G

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

A

A

R

R

L

L

G

G

A

A

D

M

V

T

M

S

M

M

V

V

T

C

R

K

V

V

G

G

D

K

D

D

V

S

F

F

A

G

D

N

N

D

T

Y

I

I

R

E

N

N

L

L

N

K

N

Q

F

N

G

D

V

I

D

S

T

T

R

E

H

F

V

T

V

Y

K

Q

I

T

S

K

G

D

K

A

S

A

S

T

G

G

V

T

A

A

P

S

I

I

F

I

V

V

E

N

P

N

S

E

G

G

E

Q

N

N

S

I

I

I

L

V

I

I

V

V

K

A

G

G

A

A

N

N

A

L

D

L

L

L

L

N

P

T

A

E

E

D

V

L

D

R

K

A

A

A

S

A

E

N

D

V

L

I

K

S

T

R

C

A

G

K

L

V

I

M

L

V

M

C

Q

Q

M

L

E

E

V

I

P

T

V

P

E

A

T

T

V

S

Y

L

H

E

T

A

I

L

S

T

F

M

A

A

A

R

E

R

N

S

G

G

I

V

E

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

A

I

A

A

D

D

L

L

D

D

P

P

E

Q

R

F

I

Y

R

T

Q

L

V

S

T

D

F

V

L

F

V

C

P

C

N

N

E

E

S

S

E

E

L

A

A

E

L

I

L

L

S

T

G

G

L

L

P

T

T

V

D

G

T

S

N

A

D

A

E

D

I

A

V

G

I

E

A

A

A

A

R

L

S

V

L

L

I

L

E

K

R

R

G

G

I

C

R

Q

T

V

V

V

I

I

V

I

T
|
T

L

L

G
|
G

G
x
A

R

E

G
|
G

A

C

R

V

M

V

I

L

T

S

A

Q

N

T

-

E

-

P

E

E

I

P

V

K

N

H

I

I

P

P

P

T

V

E

K

K

V

V

T

K

P

A

K

V

D

D

T

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

A

S

F

F

I

V

G

G

S

A

F

L

A

A

R

F

F

Y

-

L

-

A

Y

Y

A

Y

E

P

T

N

G

L

E

S

V

L

E

E

A

D

S

M

L

L

K

N

K

R

A

S

A
x
N

L

F

Y

I

A
|
A

A

A

H

V

S

S

I

V

T

Q

R

A

P

A

G

G

T

T

Q

Q

K

S

A

S

Y

Y

active site: G254 (= G251), A255 (= A252), G256 (= G253), D257 (= D254)
binding adenosine monophosphate: T223 (= T222), G225 (= G224), A226 (≠ G225), G228 (= G227), N283 (≠ A278), A286 (= A281)

Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
38% identity, 92% coverage: 9:294/312 of query aligns to 22:311/322 of Q9H477

query
sites
Q9H477

G

G

S

S

N

C

M

M

V

T

D

D

L

L

I

V

T

S

Y

L

V

T

N

S

R

R

M

L

P

P

G

K

P

T

G

G

E

E

T

T

L

I

E

H

A

G

P

H

T

K

F

F

E

F

I

I

G

G

C

F

G

G

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

A

A

R

R

L

L

G

G

A

A

D

M

V

T

M

S

M

M

V

V

T

C

R

K

V

V

G

G

D

K

D

D

V

S

F

F

A

G

D

N

N

D

T

Y

I

I

R

E

N

N

L

L

N

K

N

Q

F

N

G

D

V

I

D

S

T

T

R

E

H

F

V

T

V

Y

K

Q

I

T

S

K

G

D

K

A

S

A

S

T

G

G

V

T

A

A

P

S

I

I

F

I

V

V

E

N

P

N

S

E

G

G

E

Q

N

N

S

I

I

I

L

V

I

I

V

V

K

A

G

G

A

A

N

N

A

L

D

L

L

L

L

N

P

T

A

E

E

D

V

L

D

R

K

A

A

A

S

A

E

N

D

V

L

I

K

S

T

R

C

A

G

K

L

V

I

M

L

V

M

C

Q

Q

M

L

E

E

V

I

P

T

V

P

E

A

T

T

V

S

Y

L

H

E

T

A

I

L

S

T

F

M

A

A

A

R

E

R

N

S

G

G

I

V

E

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

A

A

A

I

A

A

D

D

L

L

D

D

P

P

E

Q

R

F

I

Y

R

T

Q

L

V

S

T

D

F

V

L

F

V

C

P

C

N
|
N

E

E

S

S

E

E

L

A

A

E

L

I

L

L

S

T

G

G

L

L

P

T

T

V

D

G

T

S

N

A

D

A

E

D

I

A

V

G

I

E

A

A

A

A

R

L

S

V

L

L

I

L

E

K

R

R

G

G

I

C

R

Q

T

V

V

V

I

I

V

I

T
|
T

L
|
L

G
|
G

G
x
A

R
x
E

G
|
G

A

C

R

V

M

V

I

L

T

S

A

Q

N

T

-

E

-

P

E

E

I

P

V

K

N

H

I

I

P

P

P
x
T

V

E

K

K

V

V

T

K

P

A

K

V

D

D

T

T

T

T

G

G

A

A

G
|
G

D
|
D

A

S

F

F

I

V

G

G

S

A

F

L

A

A

R

F

F

Y

-

L

-

A

Y

Y

A

Y

E

P

T

N

G

L

E

S

V

L

E

E

A

D

S

M

L

L

K

N

K

R

A

S

A
x
N

L

F

Y

I

A

A

A

A

H

V

S

S

I

V

T

Q

R

A

P

A

G

G

T

T

Q

Q

K

S

A

S

Y

Y

N199 (= N186) binding ATP
TLGAEG 235:240 (≠ TLGGRG 222:227) binding ATP
T256 (≠ P241) binding ATP
GD 268:269 (= GD 253:254) binding ATP
N295 (≠ A278) binding ATP

8cqxA Ribokinase from t.Sp mutant a92g
39% identity, 96% coverage: 5:304/312 of query aligns to 2:298/300 of 8cqxA

query
sites
8cqxA

I

I

G

L

V

V

V

V

G

G

S

S

N

L

M

N

V

M

D

D

L

L

I

V

T

L

Y

R

V

V

N

K

R

R

M

L

P

P

G

R

P

P

G

G

E

E

T

T

L

V

E

L

A

G

P

E

T

D

F

Y

E

Q

I

T

G

H

C

P

G

G

G

G

K

K

G

G

A

A

N

N

Q

Q

A

A

V

V

A

A

A

I

A

A

R

R

L

L

G

G

A

G

D

K

V

V

M

R

M

M

V

L

T

G

R

R

V

V

G

G

D

E

D

D

V

P

F

F

A

G

D

Q

N

A

T

L

I

K

R

S

N

G

L

L

N

A

N

Q

F

E

G

G

V

V

D

D

T

V

R

A

H

W

V

V

V

L

K

E

I

T

S

P

G

G

K

P

S

-

S

S

G

G

V

T

A

G

P

F

I

I

F

L

V

V

E

D

P

P

S

E

G

G

E

Q

N

N

S

Q

I

I

L

A

I

V

V

A

K

P

G

G

A

A

N

N

A

A

D

R

L

L

L

V

P

P

A

E

E

-

V

-

D

D

K

L

A

P

S

A

E

T

D

A

L

F

K

Q

T

G

C

V

G

G

L

V

I

V

L

L

M

L

Q

Q

M

L

E

E

V

I

P

P

V

L

E

E

T

T

V

V

Y

V

H

R

T

A

I

A

S

A

F

L

A

G

A

R

E

K

N

A

G

G

I

A

E

R

T

I

I

L

L

L

N

N

P

A

A

A

P

P

A

A

A

H

A

A

D

-

L

L

D

P

P

S

E

E

R

I

I

L

R

Q

Q

S

V

V

T

D

F

L

L

L

V

L

P

V

N
|
N

E

E

S

V

E

E

L

A

A

A

L

Q

L

L

S

T

-

E

G

A

L

S

P

P

T

P

D

R

T

T

N

P

D

E

E

E

I

A

V

L

I

A

A

L

A

A

R

R

S

Q

L

L

I

R

E

G

R

R

G

A

I

P

R

Q

T

A

-

Q

V

V

I

V

V

L

T
|
T

L

L

G
|
G

G
x
A

R

Q

G
|
G

A

A

R

-

M

V

I

W

T

S

A

G

N

T

E

E

I

E

V

S

N

H

I

F

P

P

P

A

V

F

K

P

V

V

T

R

P

A

K

V

D
|
D

T

T

T
|
T

G

A

A

A

G

G

D

D

A

A

F
|
F

I

A

G

G

S

A

F

L

A

A

R

L

F

G

Y

L

A

A

E

E

T

G

G

Q

E

N

V

M

E

R

A

A

S

A

L

L

K

R

K

F

A

A

A
x
N

L

A

Y

A

A
x
G

A
|
A

H

L

S
x
A

I
x
T

T

T

R

R

P

P

G

G

T

A

Q

Q

K

P

A
x
S

Y

L

A

P

S

F

A

R

E

D

E

E

F

V

E

E

T

A

F

L

C

L

binding adenosine-5'-diphosphate: N179 (= N186), T217 (= T222), G219 (= G224), A220 (≠ G225), G222 (= G227), F250 (= F256), N272 (≠ A278), G275 (≠ A281), A276 (= A282), T279 (≠ I285)
binding magnesium ion: D242 (= D248), T244 (= T250), A278 (≠ S284), S287 (≠ A293)

1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
38% identity, 96% coverage: 7:304/312 of query aligns to 6:302/305 of 1rk2A

query
sites
1rk2A

V

V

V

L

G

G

S

S

N

I

M
x
N

V

A

D
|
D

L

H

I

I

T

L

Y

N

V

L

N

Q

R

S

M

F

P

P

G

T

P

P

G

G

E

E

T

T

L

V

E

T

A

G

P

N

T

H

F

Y

E

Q

I

V

G

A

C

F

G
|
G

G
|
G

K
|
K

G

G

A

A

N
|
N

Q

Q

A

A

V

V

A

A

A

A

A

G

R

R

L

S

G

G

A

A

D

N

V

I

M

A

M

F

V

I

T

A

R

C

V

T

G

G

D

D

D

D

V

S

F

I

A

G

D

E

N

S

T

V

I

R

R

Q

N

Q

L

L

N

A

N

T

F

D

G

N

V

I

D

D

T

I

R

T

H

P

V

V

V

S

K

V

I

I

S

K

G

G

K

E

S

S

S

T

G

G

V

V

A

A

P

L

I

I

F

F

V

V

E

N

P

G

S

E

G

G

E

E

N

N

S

V

I

I

L

G

I

I

V

H

K

A

G

G

A

A

N

N

A

A

D

A

L

L

L

S

P

P

A

A

E

L

V

V

D

E

K

A

A

Q

S

R

E

E

D

R

L

I

K

A

T

N

C

A

G

S

L

A

I

L

L

L

M

M

Q

Q

M

L

E
|
E

V

S

P

P

V

L

E

E

T

S

V

V

Y

M

H

A

T

A

I

A

S

K

F

I

A

A

A

H

E

Q

N

N

G

K

I

T

E

I

T

V

I

A

L

L

N

N

P

P

A

A

P

P

A

-

A

A

A

R

D

E

L

L

D

P

P

D

E

E

R

L

I

L

R

A

Q

L

V

V

T

D

F

I

L

I

V

T

P

P

N

N

E

E

S

T

E

E

L

A

A

E

L

K

L

L

S

T

G

G

L

I

P

R

T

V

D

E

T

N

N

D

D

E

E

D

I

A

V

A

I

K

A

A

A

A

R

Q

S

V

L

L

I

H

E

E

R

K

G
|
G

I

I

R
|
R

T

T

V

V

I

L

V

I

T
|
T

L

L

G
|
G

G
x
S

R

R

G

G

A

V

R

W

M

A

I

S

T

V

A

N

N

G

E

E

I

G

V

Q

N

R

I

V

P

P

P

G

V

F

K

R

V

V

T

Q

P

A

K

V

D
|
D

T

T

T

I

G
x
A

A
|
A

G
|
G

D
|
D

A

T

F

F

I

N

G

G

S

A

F

L

A

I

R

T

F

A

Y

L

A

L

E

E

T

E

G

K

E

P

V

L

E

P

A

E

S

A

L

I

K

R

K

F

A

A

A
x
H

L

A

Y

A

A
|
A

A

A

H

I

S
x
A

I

V

T

T

R
|
R

P

K

G

G

T

A

Q

Q

K

P

A
x
S

Y

V

A

P

S

W

A

R

E

E

E

E

F

I

E

D

T

A

F

F

C

L

active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
binding adenosine-5'-diphosphate: T220 (= T222), G222 (= G224), S223 (≠ G225), A250 (= A252), G251 (= G253), H276 (≠ A278), A279 (= A281)
binding tetrafluoroaluminate ion: G213 (= G215), R215 (= R217)
binding magnesium ion: D246 (= D248), A282 (≠ S284), R285 (= R287), S291 (≠ A293)
binding alpha-D-ribofuranose: N11 (≠ M12), D13 (= D14), G38 (= G39), G39 (= G40), K40 (= K41), N43 (= N44), E140 (= E141), D252 (= D254)

P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 96% coverage: 7:304/312 of query aligns to 9:305/309 of P0A9J6

query
sites
P0A9J6

V

V

V

L

G

G

S

S

N

I

M
x
N

V
x
A

D
|
D

L

H

I

I

T

L

Y

N

V

L

N

Q

R

S

M

F

P

P

G

T

P

P

G

G

E

E

T

T

L

V

E

T

A

G

P

N

T

H

F

Y

E

Q

I

V

G

A

C

F

G

G

G
|
G

K
|
K

G
|
G

A
|
A

N
|
N

Q

Q

A

A

V

V

A

A

A

A

A

G

R

R

L

S

G

G

A

A

D

N

V

I

M

A

M

F

V

I

T

A

R

C

V

T

G

G

D

D

D

D

V

S

F

I

A

G

D

E

N

S

T

V

I

R

R

Q

N

Q

L

L

N

A

N

T

F

D

G

N

V

I

D

D

T

I

R

T

H

P

V

V

V

S

K

V

I

I

S

K

G

G

K

E

S

S

S

T

G

G

V

V

A

A

P

L

I

I

F

F

V

V

E

N

P

G

S

E

G

G

E

E

N

N

S

V

I

I

L

G

I

I

V

H

K

A

G

G

A

A

N

N

A

A

D

A

L

L

L

S

P

P

A

A

E

L

V

V

D

E

K

A

A

Q

S

R

E

E

D

R

L

I

K

A

T

N

C

A

G

S

L

A

I

L

L

L

M

M

Q

Q

M

L

E
|
E

V

S

P

P

V

L

E

E

T

S

V

V

Y

M

H

A

T

A

I

A

S

K

F

I

A

A

A

H

E

Q

N

N

G

K

I

T

E

I

T

V

I

A

L

L

N

N

P

P

A

A

P

P

A

-

A

A

A

R

D

E

L

L

D

P

P

D

E

E

R

L

I

L

R

A

Q

L

V

V

T

D

F

I

L

I

V

T

P

P

N
|
N

E

E

S

T

E

E

L

A

A

E

L

K

L

L

S

T

G

G

L

I

P

R

T

V

D

E

T

N

N

D

D

E

E

D

I

A

V

A

I

K

A

A

A

A

R

Q

S

V

L

L

I

H

E

E

R

K

G

G

I

I

R

R

T

T

V

V

I

L

V

I

T
|
T

L
|
L

G
|
G

G
x
S

R
|
R

G
|
G

A

V

R

W

M

A

I

S

T

V

A

N

N

G

E

E

I

G

V

Q

N

R

I

V

P

P

P

G

V

F

K

R

V

V

T

Q

P

A

K

V

D

D

T

T

T

I

G

A

A

A

G
|
G

D
|
D

A

T

F

F

I

N

G

G

S

A

F

L

A

I

R

T

F

A

Y

L

A

L

E

E

T

E

G

K

E

P

V

L

E

P

A

E

S

A

L

I

K

R

K

F

A

A

A
x
H

L

A

Y

A

A

A

A

A

H

I

S

A

I

V

T

T

R

R

P

K

G

G

T

A

Q

Q

K

P

A

S

Y

V

A

P

S

W

A

R

E

E

E

E

F

I

E

D

T

A

F

F

C

L

NAD 14:16 (≠ MVD 12:14) binding substrate
GKGAN 42:46 (= GKGAN 40:44) binding substrate
E143 (= E141) binding substrate
N187 (= N186) binding ATP
TLGSRG 223:228 (≠ TLGGRG 222:227) binding ATP
GD 254:255 (= GD 253:254) binding ATP
D255 (= D254) binding substrate
H279 (≠ A278) binding ATP

1gqtB Activation of ribokinase by monovalent cations (see paper)
38% identity, 96% coverage: 7:304/312 of query aligns to 8:304/307 of 1gqtB

query
sites
1gqtB

V

V

V

L

G

G

S

S

N

I

M
x
N

V

A

D
|
D

L

H

I

I

T

L

Y

N

V

L

N

Q

R

S

M

F

P

P

G

T

P

P

G

G

E

E

T

T

L

V

E

T

A

G

P

N

T

H

F

Y

E

Q

I

V

G

A

C

F

G

G

G
|
G

K

K

G

G

A

A

N
|
N

Q

Q

A

A

V

V

A

A

A

A

A

G

R

R

L

S

G

G

A

A

D

N

V

I

M

A

M

F

V

I

T

A

R

C

V

T

G

G

D

D

D

D

V

S

F

I

A

G

D

E

N

S

T

V

I

R

R

Q

N

Q

L

L

N

A

N

T

F

D

G

N

V

I

D

D

T

I

R

T

H

P

V

V

V

S

K

V

I

I

S

K

G

G

K

E

S

S

S

T

G

G

V

V

A

A

P

L

I

I

F

F

V

V

E

N

P

G

S

E

G

G

E

E

N

N

S

V

I

I

L

G

I

I

V

H

K

A

G

G

A

A

N

N

A

A

D

A

L

L

L

S

P

P

A

A

E

L

V

V

D

E

K

A

A

Q

S

R

E

E

D

R

L

I

K

A

T

N

C

A

G

S

L

A

I

L

L

L

M

M

Q

Q

M

L

E
|
E

V

S

P

P

V

L

E

E

T

S

V

V

Y

M

H

A

T

A

I

A

S

K

F

I

A

A

A

H

E

Q

N

N

G

K

I

T

E

I

T

V

I

A

L

L

N

N

P

P

A

A

P

P

A

-

A

A

A

R

D

E

L

L

D

P

P

D

E

E

R

L

I

L

R

A

Q

L

V

V

T

D

F

I

L

I

V

T

P

P

N
|
N

E

E

S

T

E

E

L

A

A

E

L

K

L

L

S

T

G

G

L

I

P

R

T

V

D

E

T

N

N

D

D

E

E

D

I

A

V

A

I

K

A

A

A

A

R

Q

S

V

L

L

I

H

E

E

R

K

G

G

I

I

R

R

T

T

V

V

I

L

V

I

T
|
T

L

L

G
|
G

G
x
S

R

R

G

G

A

V

R

W

M

A

I

S

T

V

A

N

N

G

E

E

I

G

V

Q

N

R

I

V

P

P

P

G

V

F

K

R

V

V

T

Q

P

A

K

V

D
|
D

T

T

T
x
I

G
x
A

A
|
A

G
|
G

D
|
D

A

T

F

F

I

N

G

G

S

A

F

L

A

I

R

T

F

A

Y

L

A

L

E

E

T

E

G

K

E

P

V

L

E

P

A

E

S

A

L

I

K

R

K

F

A

A

A
x
H

L

A

Y

A

A
|
A

A

A

H

I

S
x
A

I

V

T

T

R
|
R

P

K

G

G

T

A

Q

Q

K

P

A
x
S

Y

V

A

P

S

W

A

R

E

E

E

E

F

I

E

D

T

A

F

F

C

L

active site: A251 (≠ G251), A252 (= A252), G253 (= G253), D254 (= D254)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N186), T222 (= T222), G224 (= G224), S225 (≠ G225), A252 (= A252), G253 (= G253), H278 (≠ A278), A281 (= A281)
binding cesium ion: D248 (= D248), I250 (≠ T250), A284 (≠ S284), R287 (= R287), S293 (≠ A293)
binding alpha-D-ribofuranose: N13 (≠ M12), D15 (= D14), G41 (= G40), N45 (= N44), E142 (= E141), D254 (= D254)

6a8cA Ribokinase from leishmania donovani with adp (see paper)
38% identity, 95% coverage: 5:300/312 of query aligns to 16:322/327 of 6a8cA

query
sites
6a8cA

I

I

G

L

V

V

V

V

G

G

S

S

N

C

M

F

V

L

D
|
D

L

Y

I

V

T

G

Y

Y

V

V

N

D

R

H

M

M

P

P

G

Q

P

V

G

G

E

E

T

T

L

M

E

H

A
x
S

P

V

T
x
S

F

F

E

H

I

K

G

G

C

F

G
|
G

G
|
G

K

K

G

G

A

A

N
|
N

Q

Q

A

A

V

V

A

A

A

A

A

G

R

R

L

L

G

G

A

A

D

K

V

V

M

A

M

M

V

V

T

S

R

M

V

V

G

G

D

T

D

D

V

G

F

D

A

G

D

S

N

D

T

Y

I

I

R

K

N

E

L

L

N

E

N

R

F

N

G

G

V

V

D

D

T

T

R

A

H

Y

V

M

V

F

K

R

I

T

S

G

G

K

K

S

S

S

S

T

G

G

V

L

A

A

P

M

I

I

F

L

V

V

E

D

-

T

P

K

S

S

G

S

E

N

N

N

S

E

I

I

L

V

I

I

V

C

K

P

G

N

A

A

-

T

-

N

-

H

-

F

N

T

A

P

D

E

L

L

L

L

P

R

A

A

E

Q

V

T

D

N

K

N

A

Y

S

E

E

R

D

I

L

L

K

H

T

T

C

-

G

G

L

L

-

K

-

Y

I

L

L

I

M

C

Q

Q

M

N

E

E

V

I

P

P

V

L

E

P

T

T

V

T

Y

L

H

D

T

T

I

I

S

K

F

E

A

A

A

H

E

S

N

R

G

G

I

V

E

Y

T

T

I

V

L

F

N

N

P

S

A

A

P

P

A

A

A

P

A

K

D

P

L

A

D

E

P

V

E

E

R

Q

I

I

R

K

-

P

-

F

-

L

-

P

Q

Y

V

V

T

S

F

L

L

F

V

C

P

P

N

N

E

E

S

V

E

E

L

A

A

T

L

L

L

I

S

T

G

G

L

V

P

K

T

V

D

T

T

D

N

T

D

E

E

S

I

A

V

F

I

S

A

A

A

I

R

K

S

A

L

L

I

Q

E

Q

R

L

G

G

I

V

R

R

T

D

V

V

I

V

V

I

T

T

L

L

G
|
G

G
x
A

R

A

G

G

A

F

R

-

M

V

I

L

T

S

A

E

N

N

-

G

-

A

E

E

I

P

V

V

N

H

I

V

P

T

P

G

V

K

K

H

V

V

T

K

P

A

K

V

D

D

T
|
T

T

T

G

G

A
|
A

G
|
G

D

D

A

C

F

F

I

V

G

G

S

S

F

M

A

V

R

Y

F

F

Y

M

A

S

E

R

T

G

G

R

E

N

V

L

E

L

A

E

S

A

L

C

K

K

K

R

A

A

A
x
N

L

E

Y

C

A
|
A

A

A

H

I

S

S

I

V

T

T

R

R

P

K

G

G

T

T

Q

Q

K

L

A

S

Y

Y

A

P

S

H

A

P

E

S

E

E

F

L

binding adenosine-5'-diphosphate: G245 (= G224), A246 (≠ G225), T271 (= T249), A274 (= A252), G275 (= G253), N300 (≠ A278), A303 (= A281)
binding glycerol: D25 (= D14), S42 (≠ A31), S44 (≠ T33), G50 (= G39), G51 (= G40), N55 (= N44)

6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
38% identity, 95% coverage: 5:300/312 of query aligns to 16:322/327 of 6a8bA

query
sites
6a8bA

I

I

G

L

V

V

V

V

G

G

S

S

N

C

M

F

V

L

D
|
D

L

Y

I

V

T

G

Y

Y

V

V

N

D

R

H

M

M

P

P

G

Q

P

V

G

G

E

E

T

T

L

M

E

H

A

S

P

V

T

S

F

F

E

H

I

K

G

G

C

F

G
|
G

G
|
G

K

K

G

G

A

A

N
|
N

Q

Q

A

A

V

V

A

A

A

A

A

G

R

R

L

L

G

G

A

A

D

K

V

V

M

A

M

M

V

V

T

S

R

M

V

V

G

G

D

T

D

D

V

G

F

D

A

G

D

S

N

D

T

Y

I

I

R

K

N

E

L

L

N

E

N

R

F

N

G

G

V

V

D

D

T

T

R

A

H

Y

V

M

V

F

K

R

I

T

S

G

G

K

K

S

S

S

S

T

G

G

V

L

A

A

P

M

I

I

F

L

V

V

E

D

-

T

P

K

S

S

G

S

E

N

N

N

S

E

I

I

L

V

I

I

V

C

K

P

G

N

A

A

-

T

-

N

-

H

-

F

N

T

A

P

D

E

L

L

L

L

P

R

A

A

E

Q

V

T

D

N

K

N

A

Y

S

E

E

R

D

I

L

L

K

H

T

T

C

-

G

G

L

L

-

K

-

Y

I

L

L

I

M

C

Q

Q

M
x
N

E

E

V
x
I

P

P

V

L

E

P

T

T

V

T

Y

L

H

D

T

T

I

I

S

K

F

E

A

A

A

H

E

S

N

R

G

G

I

V

E

Y

T

T

I

V

L

F

N

N

P

S

A

A

P

P

A

A

A

P

A

K

D

P

L

A

D
x
E

P

V

E

E

R

Q

I

I

R

K

-

P

-

F

-

L

-

P

Q

Y

V

V

T

S

F

L

L

F

V

C

P

P

N

N

E

E

S

V

E

E

L

A

A

T

L

L

L

I

S

T

G

G

L

V

P

K

T

V

D

T

T

D

N

T

D

E

E

S

I

A

V

F

I

S

A

A

A

I

R

K

S

A

L

L

I

Q

E

Q

R

L

G

G

I

V

R

R

T

D

V

V

I

V

V

I

T

T

L

L

G
|
G

G
x
A

R

A

G

G

A

F

R

-

M

V

I

L

T

S

A

E

N

N

-

G

-

A

E

E

I

P

V

V

N

H

I

V

P

T

P

G

V

K

K

H

V

V

T

K

P

A

K

V

D

D

T
|
T

T

T

G

G

A
|
A

G
|
G

D

D

A

C

F

F

I

V

G

G

S

S

F

M

A

V

R

Y

F

F

Y

M

A

S

E

R

T

G

G

R

E

N

V

L

E

L

A

E

S

A

L

C

K

K

K

R

A

A

A
x
N

L

E

Y

C

A
|
A

A

A

H

I

S

S

I
x
V

T

T

R

R

P

K

G

G

T

T

Q

Q

K

L

A

S

Y

Y

A

P

S

H

A

P

E

S

E

E

F

L

binding phosphomethylphosphonic acid adenylate ester: G245 (= G224), A246 (≠ G225), T271 (= T249), A274 (= A252), G275 (= G253), N300 (≠ A278), A303 (= A281), V307 (≠ I285)
binding glycerol: D25 (= D14), G50 (= G39), G51 (= G40), N55 (= N44), N157 (≠ M140), I159 (≠ V142), E190 (≠ D173)

6a8aA Ribokinase from leishmania donovani with atp (see paper)
38% identity, 95% coverage: 5:300/312 of query aligns to 16:322/327 of 6a8aA

query
sites
6a8aA

I

I

G

L

V

V

V

V

G

G

S

S

N

C

M

F

V

L

D
|
D

L

Y

I

V

T

G

Y

Y

V

V

N

D

R

H

M

M

P

P

G

Q

P

V

G

G

E

E

T

T

L

M

E

H

A

S

P

V

T

S

F

F

E

H

I

K

G

G

C

F

G
|
G

G
|
G

K

K

G

G

A

A

N
|
N

Q

Q

A

A

V

V

A

A

A

A

A

G

R

R

L

L

G

G

A

A

D

K

V

V

M

A

M

M

V

V

T

S

R

M

V

V

G

G

D

T

D

D

V

G

F

D

A

G

D

S

N

D

T

Y

I

I

R

K

N

E

L

L

N

E

N

R

F

N

G

G

V

V

D

D

T

T

R

A

H

Y

V

M

V

F

K

R

I

T

S

G

G

K

K

S

S

S

S

T

G

G

V

L

A

A

P

M

I

I

F

L

V

V

E

D

-

T

P

K

S

S

G

S

E

N

N

N

S

E

I

I

L

V

I

I

V

C

K

P

G

N

A

A

-

T

-

N

-

H

-

F

N

T

A

P

D

E

L

L

L

L

P

R

A

A

E

Q

V

T

D

N

K

N

A

Y

S

E

E

R

D

I

L

L

K

H

T

T

C

-

G

G

L

L

-

K

-

Y

I

L

L

I

M

C

Q

Q

M

N

E

E

V

I

P

P

V

L

E

P

T

T

V

T

Y

L

H

D

T

T

I

I

S

K

F

E

A

A

A

H

E

S

N

R

G

G

I

V

E

Y

T

T

I

V

L

F

N

N

P

S

A

A

P

P

A

A

A

P

A

K

D

P

L

A

D

E

P

V

E

E

R

Q

I

I

R

K

-

P

-

F

-

L

-

P

Q

Y

V

V

T

S

F

L

L

F

V

C

P

P

N
|
N

E

E

S

V

E

E

L

A

A

T

L

L

L

I

S

T

G

G

L

V

P

K

T

V

D

T

T

D

N

T

D

E

E

S

I

A

V

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