SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_094507891.1 NCBI__GCF_002252445.1:WP_094507891.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
30% identity, 90% coverage: 17:419/448 of query aligns to 11:420/425 of O59010

query
sites
O59010

P

P

L

V

Y

L

K

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

A

I

A

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

Y

Y

P

A

E

D

-

A

L

V

G

K

T

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T
x
S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

V

T

I

L

G

G

L

I

I

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

A

P

V

A

G

S

G

L

Q

D

Q

P

F

Q

Q

A

-

V

-

A

-

N

-

Y

-

T

-

E

-

K

P

A

K

H

Q

E

A

Q

P

T

P

I

L

M

V

G

K

F

I

L

L

T

L

N

D

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

S

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

K

S

G

A

K

E

P

T

V

L

T

L

D

D

F

A

L

I

Q

N

M

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

K

L

I

V

V

S

N

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

S

V

I

V

A

G

N

E

L

L

A

A

M

K

L

V

I

T

A

A

T

A

F

V

Y

Y

I

V

T

G

A

L

L

T

L

L

F

Q

V

I

L

L

V

L

V

V

L

Y

G

F

S

V

V

L

A

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

K

V

M

A

E

K

K

E

A

M

G

G

C

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

I

S

P

H

L

L

S

T

L

V

T

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I
x
V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E
x
M

C

G

R
|
R

A

T

L

M

T

V

N
|
N

L

V

V

T

G

G

N
x
D

A

L

V

T

A

G

T

T

I

A

V

I

V

V

A

A

R

K

W

T

E

E

N

G

E

E

L

L

D

E

S65 (≠ T69) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S280) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 280:282) binding L-aspartate
M311 (≠ L315) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T318) binding L-aspartate
V355 (≠ I359) binding L-aspartate
D394 (≠ S393) binding L-aspartate
M395 (≠ E394) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R396) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N400) binding L-aspartate
D405 (≠ N404) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
30% identity, 89% coverage: 17:415/448 of query aligns to 2:407/407 of 2nwwA

query
sites
2nwwA

P

P

L

V

Y

L

K

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

A

I

A

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

Y

Y

P

A

E

H

-

A

L

V

G

H

T

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

V

T

I

L

G

G

L

I

I

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

-

P

-

A

-

S

-

L

-

D

-

P

-

Q

-

A

A

V

V

A

G

N

G

Y

Q

T

Q

E

F

K

Q

A

P

H

H

E

Q

Q

A

T

P

-

P

I

L

M

V

G

H

F

I

L

L

T

L

N

D

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

S

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

K

S

G

A

K

E

P

T

V

L

T

L

D

D

F

A

L

I

Q

N

M

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

K

L

I

V

V

S

N

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

H

S

V

I

V

A

G

N

E

L

L

A

A

M

K

L

V

I

T

A

A

T

A

F

V

Y

Y

I

V

T

G

A

L

L

T

L

L

F

Q

V

I

L

L

V

L

V

V

L

Y

G

F

S

V

V

L

A

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

H

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

K

V

M

A

E

K

K

E

A

M

G

G

C

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

I

S

P

H

L

L

S

T

L

V

T

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A
|
A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

H

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

I

A

V

I

V

V

A

A

R

K

W

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S282), T305 (= T318), A349 (= A362), G350 (= G363), D385 (≠ S393), R388 (= R396), T389 (≠ A397), N392 (= N400)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
30% identity, 90% coverage: 17:418/448 of query aligns to 11:419/419 of 6x15A

query
sites
6x15A

P

P

L

V

Y

L

K

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

A

I

A

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

Y

Y

P

A

E

H

-

A

L

V

G

H

T

T

Q

Y

L

V

K

K

P

P

L
x
F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

C

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D
x
P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F
x
Y

L

L

T
x
L

F
x
T

S
|
S

T

A

L
x
F

A

A

L

V

V

T

I

L

G

G

L

I

I

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

-

P

-

A

-

S

-

L

-

D

-

P

-

Q

-

A

A

V

V

A

G

N

G

Y

Q

T

Q

E

F

K

Q

A

P

H

H

E

Q

Q

A

T

P

-

P

I
x
L

M

V

G

H

F
x
I

L

L

T

L

N

D

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

S

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V
x
I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

K

S

G

A

K

E

P

T

V

L

T

L

D

D

F

A

L

I

Q

N

M

G

L

L

T

A

T

E

P

A

V

M

F

Y

K
|
K

L

I

V

V

S

N

I
x
G

L

V

M

M

K

Q

A

Y

A

A

P

P

I
|
I

G

G

A

V

F
|
F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

H

S

V

I

V

A

G

N

E

L

L

A

A

M

K

L

V

I

T

A

A

T

A

F

V

Y

Y

I

V

T

G

A

L

L

T

L

L

F

Q

V

I

L

L

V

L

V

V

L

Y

G

F

S

V

V
x
L

A

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I
|
I

R

K

Y

H

I

A

K

K

E

D

E

A

L
x
M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

K

V

M

A

E

K

K

E

A

M

G

G

C

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y

A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

A

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

I

S

P

H

L

L

S
x
T

L
x
V

T
x
G

D

Q

Q

Q

I

L

L
x
T

L

I

L

V

L
|
L

V

T

A

A

M

V

L

L

S

A

S
|
S

K
x
I

G

G

A
x
T

A

A

G
|
G

I

V

T

P

G

G

A
|
A

G
|
G

F

A

I

I

T

M

L

L

A

C

A

M

T

V

L

L

S

H

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L
x
M

T

V

N
|
N

L
x
V

V

T

G

G

N
x
D

A

L

V

T

A

G

T

T

I

A

V

I

V

V

A

A

R

K

W

T

E

E

N

G

E

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L50), F46 (≠ L50), P75 (≠ D79), L91 (≠ T96), F95 (≠ L100), L130 (≠ I142), I133 (≠ F145), I159 (≠ V171), Y167 (≠ M179), K196 (= K200), G200 (≠ I204), I207 (= I211), F210 (= F214), L250 (≠ V254), I262 (= I266), M269 (≠ L273), T334 (≠ S338), V335 (≠ L339), G336 (≠ T340), T340 (≠ L344), L343 (= L347), M399 (≠ L398)
binding aspartic acid: S277 (= S281), S278 (= S282), T314 (= T318), G354 (= G358), A358 (= A362), G359 (= G363), D394 (≠ S393), R397 (= R396), T398 (≠ A397)
binding sodium ion: Y89 (≠ F94), T92 (≠ F97), S93 (= S98), G306 (= G310), T308 (≠ S312), N310 (= N314), N310 (= N314), M311 (≠ L315), D312 (= D316), S349 (= S353), I350 (≠ K354), T352 (≠ A356), N401 (= N400), V402 (≠ L401), D405 (≠ N404)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
30% identity, 89% coverage: 17:415/448 of query aligns to 8:413/413 of 6x14A

query
sites
6x14A

P

P

L

V

Y

L

K

Q

Q

K

L
x
I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

A

I

A

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

Y

Y

P

A

E

H

-

A

L

V

G

H

T

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

C

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

A

V

T

V

G
|
G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M
x
V

I

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

V

T

I

L

G

G

L

I

I

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

-

P

-

A

-

S

-

L

-

D

-

P

-

Q

-

A

A

V

V

A

G

N

G

Y

Q

T

Q

E

F

K

Q

A

P

H

H

E

Q

Q

A

T

P

-

P

I

L

M

V

G

H

F

I

L

L

T

L

N

D

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

S

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L
x
I

F

L

G

G

V

I

S

A

L

I

A

T

M
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

K

S

G

A

K

E

P

T

V

L

T

L

D

D

F

A

L

I

Q

N

M

G

L

L

T

A

T

E

P

A

V

M

F

Y

K
|
K

L

I

V

V

S

N

I

G

L

V

M
|
M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

H

S

V

I

V

A

G

N

E

L

L

A

A

M

K

L

V

I

T

A

A

T

A

F

V

Y

Y

I

V

T

G

A

L

L

T

L

L

F

Q

V

I

L

L

V

L

V

V

L

Y

G

F

S

V

V

L

A

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

H

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

K

V

M

A

E

K

K

E

A

M

G

G

C

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S
x
T

F
x
I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

A

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

I

S

P

H

L

L

S

T

L

V

T

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K
x
I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G
|
G

F

A

I

I

T

M

L

L

A

C

A

M

T

V

L

L

S

H

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L
|
L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A

T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

I

A

V

I

V

V

A

A

R

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G73), V83 (≠ M91), I157 (≠ L172), Y164 (≠ M179), K193 (= K200), T305 (≠ S312), I306 (≠ F313), I347 (≠ K354)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L22), M199 (= M206), S275 (= S282), T311 (= T318), G356 (= G363), L384 (= L386), D391 (≠ S393), R394 (= R396)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
29% identity, 89% coverage: 17:416/448 of query aligns to 3:409/409 of 6bavA

query
sites
6bavA

P

P

L

V

Y

L

K

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

A

I

A

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

Y

Y

P

A

E

D

-

A

L

V

G

K

T

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

V

T

I

L

G

G

L

I

I

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

A

P

V

A

G

S

G

L

Q

D

Q

P

F

Q

Q

A

-

V

-

A

-

N

-

Y

-

T

-

E

-

K

P

A

K

H

Q

E

A

Q

P

T

P

I

L

M

V

G

K

F

I

L

L

T

L

N

D

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

S

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

K

S

G

A

K

E

P

T

V

L

T

L

D

D

F

A

L

I

Q

N

M

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

K

L

I

V

V

S

N

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

S

V

I

V

A

G

N

E

L

L

A

A

M

K

L

V

I

T

A

A

T

A

F

V

Y

Y

I

V

T

G

A

L

L

T

L

L

F

Q

V

I

L

L

V

L

V

V

L

Y

G

F

S

V

V

L

A

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S
x
R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

K

V

M

A

E

K

K

E

A

M

G

G

C

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

N

A

I

L

Y
|
Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

I

S

P

H

L

L

S

T

L

V

T

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D
|
D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
x
C

A
x
T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

I

A

V

I

V

V

A

A

R

K

W

T

E

E

N

G

binding benzylcysteine: R268 (≠ S280), S270 (= S282), T306 (= T318), Y309 (= Y321), D382 (= D389), D386 (≠ S393), C389 (≠ R396), T390 (≠ A397)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
29% identity, 89% coverage: 17:415/448 of query aligns to 3:408/408 of 6bauA

query
sites
6bauA

P

P

L

V

Y

L

K

Q

Q

K

L

I

Y

L

V

I

Q

G

V

L

L

I

V

L

A

G

I

A

A

I

A

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

-

G

Y

Y

P

A

E

D

-

A

L

V

G

K

T

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

L

F

T

S

S

T

A

L

F

A

A

L

V

V

T

I

L

G

G

L

I

I

I

V

M

A

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

A

P

V

A

G

S

G

L

Q

D

Q

P

F

Q

Q

A

-

V

-

A

-

N

-

Y

-

T

-

E

-

K

P

A

K

H

Q

E

A

Q

P

T

P

I

L

M

V

G

K

F

I

L

L

T

L

N

D

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

S

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

K

S

G

A

K

E

P

T

V

L

T

L

D

D

F

A

L

I

Q

N

M

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

K

L

I

V

V

S

N

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

K

S

V

I

V

A

G

N

E

L

L

A

A

M

K

L

V

I

T

A

A

T

A

F

V

Y

Y

I

V

T

G

A

L

L

T

L

L

F

Q

V

I

L

L

V

L

V

V

L

Y

G

F

S

V

V

L

A

L

R

K

Y

I

N

Y

G

G

F

I

S

D

I

P

L

I

A

S

L

F

I

I

R

K

Y

K

I

A

K

K

E

D

E

A

L

M

L

L

L

T

V

A

L

F

G

V

T

T

S

R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

K

V

M

A

E

K

K

E

A

M

G

G

C

I

K

S

R

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

I

S

P

H

L

L

S

T

L

V

T

G

D

Q

Q

Q

I

L

L

T

L

I

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A
|
A

G
|
G

I
x
V

T

P

G

G

A

A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

E

V

S

V

V

-

G

-

L

-

P

-

L

-

T

-

D

P

P

A

N

V

V

P

-

V

A

A

A

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
x
C

A
x
T

L

M

T

V

N
|
N

L

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

I

A

V

I

V

V

A

A

R

K

W

T

E

E

binding cysteine: S270 (= S282), M303 (≠ L315), T306 (= T318), A345 (= A357), G346 (= G358), V347 (≠ I359), G351 (= G363), D386 (≠ S393), C389 (≠ R396), T390 (≠ A397), N393 (= N400)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
31% identity, 90% coverage: 18:422/448 of query aligns to 9:421/425 of 6zgbA

query
sites
6zgbA

L

L

Y

W

K

K

Q

I

L

L

Y

W

V

G

Q

L

V

V

L

L

V

G

A

A

I

V

A

F

A

-

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

E

A

L

V

G

K

T

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

V

F

I

F

G

G

L

L

I

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

-

M

-

H

N

I

V

D

N

P

L

A

G

S

S

L

G

D

T

P

G

Q

K

A

A

V

I

A

-

N

-

Y

-

T

-

E

-

K

E

A

A

H

Q

E

P

Q

P

T

S

I

L

M

V

G

Q

F

T

L

L

T

L

N

N

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

S

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

V

L

G

M

E

N

K

R

G

N

-

E

-

R

-

V

-

R

K

K

P

S

V

A

T

E

D

T

F

L

L

L

Q

R

M

V

-

F

-

D

-

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

L

L

I

V

V

S

G

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

S

V

I

V

A

G

N

P

L

L

A

A

M

K

L

V

I

V

A

G

T

A

F

V

Y

Y

I

T

T

G

A

L

L

F

L

L

F

Q

V

I

L

V

V

I

V

T

L

Y

G

F

S

I

V

L

A

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

C

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T

T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

I

H

P

P

L

L

S

T

L

L

T

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

I

V

V

I

V

V

A

A

R

K

W

T

E

E

N

K

E

E

L

L

D

D

T

E

E

S

R

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S280), S273 (= S281), S274 (= S282), M308 (≠ L315), D392 (≠ S393), R395 (= R396), T396 (≠ A397)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
31% identity, 90% coverage: 18:422/448 of query aligns to 11:423/426 of 6xwnB

query
sites
6xwnB

L

L

Y

W

K

K

Q

I

L

L

Y

W

V

G

Q

L

V

V

L

L

V

G

A

A

I

V

A

F

A

-

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

E

A

L

V

G

K

T

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

V

F

I

F

G

G

L

L

I

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

-

M

-

H

N

I

V

D

N

P

L

A

G

S

S

L

G

D

T

P

G

Q

K

A

A

V

I

A

-

N

-

Y

-

T

-

E

-

K

E

A

A

H

Q

E

P

Q

P

T

S

I

L

M

V

G

Q

F

T

L

L

T

L

N

N

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

S

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

V

L

G

M

E

N

K

R

G

N

-

E

-

R

-

V

-

R

K

K

P

S

V

A

T

E

D

T

F

L

L

L

Q

R

M

V

-

F

-

D

-

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

L

L

I

V

V

S

G

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

S

V

I

V

A

G

N

P

L

L

A

A

M

K

L

V

I

V

A

G

T

A

F

V

Y

Y

I

T

T

G

A

L

L

F

L

L

F

Q

V

I

L

V

V

I

V

T

L

Y

G

F

S

I

V

L

A

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S

R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

C

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

I

H

P

P

L

L

S

T

L

L

T

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G

G

A

A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A

T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

I

V

V

I

V

V

A

A

R

K

W

T

E

E

N

K

E

E

L

L

D

D

T

E

E

S

R

K

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S281), S276 (= S282), M310 (≠ L315), T313 (= T318), D394 (≠ S393), R397 (= R396)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
31% identity, 90% coverage: 18:422/448 of query aligns to 8:420/424 of 6zl4A

query
sites
6zl4A

L

L

Y

W

K

K

Q

I

L

L

Y

W

V

G

Q

L

V

V

L

L

V

G

A

A

I

V

A

F

A

-

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

E

A

L

V

G

K

T

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

V

F

I

F

G

G

L

L

I

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

-

M

-

H

N

I

V

D

N

P

L

A

G

S

S

L

G

D

T

P

G

Q

K

A

A

V

I

A

-

N

-

Y

-

T

-

E

-

K

E

A

A

H

Q

E

P

Q

P

T

S

I

L

M

V

G

Q

F

T

L

L

T

L

N

N

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

S

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

V

L

G

M

E

N

K

R

G

N

-

E

-

R

-

V

-

R

K

K

P

S

V

A

T

E

D

T

F

L

L

L

Q

R

M

V

-

F

-

D

-

G

L

L

T

A

T

E

P

A

V

M

F

Y

K
x
L

L

I

V

V

S

G

I
x
G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

S

V

I

V

A

G

N

P

L

L

A

A

M

K

L

V

I

V

A

G

T

A

F

V

Y

Y

I

T

T

G

A

L

L

F

L

L

F

Q

V

I

L

V

V

I

V

T

L

Y

G

F

S

I

V

L

A

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D
x
R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

C

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

I

H

P

P

L

L

S

T

L

L

T

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

T

P

G
|
G

A
|
A

G

G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S

D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

I

V

V

I

V

V

A

A

R

K

W

T

E

E

N

K

E

E

L

L

D

D

T

E

E

S

R

K

binding decyl-beta-d-maltopyranoside: L191 (≠ K200), G195 (≠ I204), R282 (≠ D291)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S280), S272 (= S281), S273 (= S282), M307 (≠ L315), T310 (= T318), G353 (= G361), A354 (= A362), R394 (= R396), T395 (≠ A397)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
31% identity, 90% coverage: 18:422/448 of query aligns to 11:423/427 of 5e9sA

query
sites
5e9sA

L

L

Y

W

K

K

Q

I

L

L

Y

W

V

G

Q

L

V

V

L

L

V

G

A

A

I

V

A

F

A

-

G

G

I

L

L

I

L

A

G

G

H

H

F

F

-

G

Y

Y

P

A

-

G

E

A

L

V

G

K

T

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F
x
Y

L

L

T

A

F
x
T

S
|
S

T

A

L

M

A

A

L

V

V

F

I

F

G

G

L

L

I

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

-

M

-

H

N

I

V

D

N

P

L

A

G

S

S

L

G

D

T

P

G

Q

K

A

A

V

I

A

-

N

-

Y

-

T

-

E

-

K

E

A

A

H

Q

E

P

Q

P

T

S

I

L

M

V

G

Q

F

T

L

L

T

L

N

N

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

S

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

V

L

G

M

E

N

K

R

G

N

-

E

-

R

-

V

-

R

K

K

P

S

V

A

T

E

D

T

F

L

L

L

Q

R

M

V

-

F

-

D

-

G

L

L

T

A

T

E

P

A

V

M

F

Y

K
x
L

L

I

V

V

S

G

I
x
G

L

V

M

M

K

Q

A
x
Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

S

V

I

V

A

G

N

P

L

L

A

A

M

K

L

V

I

V

A

G

T

A

F

V

Y

Y

I

T

T

G

A

L

L

F

L

L

F

Q

V

I

L

V

V

I

V

T

L

Y

G

F

S

I

V

L

A

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

C

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

N

A

I

L

Y

Y

M

Q

T

G

L

V

A

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

I

H

P

P

L

L

S

T

L

L

T

G

D

Q

Q

Q

I

L

L

V

L

L

V

T

A

A

M

V

L

L

S

A

S
|
S

K
x
I

G
|
G

A
x
T

A

A

G
|
G

I
x
V

T

P

G

G

A
|
A

G
|
G

F

A

I

I

T

M

L

L

A

A

A

M

T

V

L

L

S

Q

V

S

V

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

V

S

P

P

V

V

A

A

-

L

G

A

M

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

C

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L
x
V

V

T

G

G

N
x
D

A

L

V

A

A

G

T

T

I

V

V

I

V

V

A

A

R

K

W

T

E

E

N

K

E

E

L

L

D

D

T

E

E

S

R

K

binding aspartic acid: R274 (≠ S280), S275 (= S281), S276 (= S282), T313 (= T318), G353 (= G358), V354 (≠ I359), A357 (= A362), G358 (= G363), D394 (≠ S393), R397 (= R396), T398 (≠ A397)
binding decyl-beta-d-maltopyranoside: L194 (≠ K200), G198 (≠ I204), Y202 (≠ A208)
binding sodium ion: Y87 (≠ F94), T90 (≠ F97), S91 (= S98), S276 (= S282), G305 (= G310), A306 (≠ Y311), T307 (≠ S312), N309 (= N314), N309 (= N314), M310 (≠ L315), D311 (= D316), S348 (= S353), I349 (≠ K354), G350 (= G355), T351 (≠ A356), N401 (= N400), V402 (≠ L401), D405 (≠ N404)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
30% identity, 90% coverage: 18:422/448 of query aligns to 4:412/416 of 6r7rA

query
sites
6r7rA

L

L

Y

W

K

K

Q

I

L

L

Y

W

V

G

Q

L

V

V

L

L

V

G

A

A

I

V

A

F

A

-

G

G

I

L

L

I

L

A

G

G

H
|
H

F

F

-

G

-

Y

Y

A

P

G

E

A

L

V

G

K

T

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

V

L

K

K

M

M

I

L

I

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

A

V

T

V

G

G

I

A

A

A

G

S

M

I

T

S

D

P

M

-

K

A

K

R

V

L

G

G

R

R

V

V

A

G

G

V

K

K

A

I

M

V

I

V

Y

Y

F

Y

L

L

T

A

F

T

S

S

T

A

L

M

A

A

L

V

V

F

I

F

G

G

L

L

I

I

V

V

A

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

M

V

H

N

I

L

D

G

P

S

A

G

S

T

L

-

D

-

P

-

Q

-

A

-

V

-

A

-

N

-

Y

-

T

-

E

-

K

G

A

A

H

Q

E

P

Q

P

T

S

I

L

M

V

G

Q

F

T

L

L

T

L

N

N

I

I

I

V

P

P

T

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

S

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

F

F

S

A

V

I

L

I

F

L

G

G

V

I

S

A

L

I

A

T

M

Y

V

L

G

M

E

N

K

R

G

N

-

E

-

R

-

V

-

R

K

K

P

S

V

A

T

E

D

T

F

L

L

L

Q

R

M

V

-

F

-

D

-

G

L

L

T

A

T

E

P

A

V

M

F

Y

K

L

L

I

V

V

S

G

I

G

L

V

M

M

K

Q

A

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

I

V

G

R

S

V

I
x
V

A

G

N

P

L

L

A
|
A

M

K

L

V

I

V

A

G

T

A

F

V

Y

Y

I

T

T

G

A

L

L

F

L

L

F

Q

V

I

L

V

V

I

V

T

L

Y

G

F

S

I

V

L

A

L

R

K

Y

V

N

F

G

G

F

I

S

D

I

P

L

I

A

K

L

F

I

I

R

R

Y

K

I

A

K

K

E

D

E

A

L

M

L

I

L

T

V

A

L

F

G

V

T

T

S
x
R

S

S

S
|
S

E

S

A

G

A

T

L

L

P

P

S

V

L

T

M

M

D

R

-

V

K

A

M

E

E

E

K

E

A

M

G

G

C

V

K

D

R

K

S

G

V

I

V

F

G

S

L

F

V

T