SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_095190355.1 NCBI__GCF_003031005.1:WP_095190355.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
27% identity, 93% coverage: 3:407/437 of query aligns to 6:420/425 of O59010

query
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O59010

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S65 (≠ V56) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A268) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 268:270) binding L-aspartate
M311 (≠ L303) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ F306) binding L-aspartate
V355 (≠ I347) binding L-aspartate
D394 (≠ G381) binding L-aspartate
M395 (≠ I382) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R384) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N388) binding L-aspartate
D405 (≠ N392) mutation to N: Strongly decreased affinity for aspartate.

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
26% identity, 93% coverage: 3:410/437 of query aligns to 1:420/424 of 6zl4A

query
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6zl4A

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binding decyl-beta-d-maltopyranoside: L3 (≠ C5), L191 (≠ R188), G195 (≠ M192), R282 (= R279)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A268), S272 (= S269), S273 (= S270), M307 (≠ L303), T310 (≠ F306), G353 (= G349), A354 (≠ S350), R394 (= R384), T395 (≠ A385)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
26% identity, 93% coverage: 3:410/437 of query aligns to 2:421/425 of 6zgbA

query
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6zgbA

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L

-

T

P

P

A

G

I

S

P

P

V

V

-

A

V

L

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A
x
T

L

M

T

V

N

N

L

V

I

T

G

G

N

D

C

L

V

A

A

G

T

T

V

V

A

I

I

V

A

A

R

K

W

T

E

E

K

K

D

E

I

L

D

D

V

E

Q

S

R

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A268), S273 (= S269), S274 (= S270), M308 (≠ L303), D392 (≠ G381), R395 (= R384), T396 (≠ A385)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
26% identity, 93% coverage: 3:410/437 of query aligns to 4:423/427 of 5e9sA

query
sites
5e9sA

R

R

W

Y

C

L

S

D

R

Y

S

P

I

V

F

L

L

W

Q

K

V

I

V

L

L

W

G

G

L

L

V

V

L

L

G

G

I

A

V

V

C

F

G

G

L

L

T

I

L

A

P

G

E

H

Y

F

-

G

-

Y

-

A

-

G

-

A

-

V

S

K

A

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

L

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F
x
Y

E

L

V

A

L
x
T

T
x
S

T

A

I

M

A

A

L

V

V

F

I

F

G

G

L

L

V

I

F

V

A

G

F

R

S

L

T

F

G

N

I

V

G

G

S

A

G

N

A

V

N

N

I

L

H

-

L

-

E

-

Q

-

L

-

S

-

A

G

A

S

D

G

M

T

G

G

D

K

L

A

A

I

E

E

R

-

S

-

Q

A

H

Q

M

P

H

P

T

S

T

L

T

V

Q

Q

F

T

L

L

M

L

D

N

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

A

A

S

F

L

A

A

D

K

N

G

N

E

I

V

L

L

Q

P

V

V

L

I

L

F

F

F

S

A

V

I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

R

L

V

I

F

N

D

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R
x
L

I

I

M

V

G

G

M
x
G

I

V

V

M

R

Q

L
x
Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

R

S

V

L

V

Q

G

H

P

L

L

G

A

S

K

L

V

V

V

G

G

L

A

F

V

Y

Y

L

T

T

G

C

L

I

F

A

L

F

Q

V

I

S

V

V

I

I

T

L

Y

G

F

V

I

V

L

M

L

R

K

A

V

S

F

G

G

L

I

R

D

M

P

W

I

P

K

L

F

L

I

K

R

Y

K

L

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

M

F

G

V

T

T

A
x
R

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

-

V

K

A

L

E

E

E

H

E

L

M

G

G

I

V

G

D

S

K

S

G

T

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

F
x
T

S

A

I

L

Y

Y

L

Q

T

G

L

V

A

T

I

V

V

L

F

F

I

V

A

A

N

N

A

A

T

I

G

G

T

H

P

P

L

L

A

T

M

L

T

G

D

Q

L

Q

L

L

T

V

I

V

L

V

L

L

V

T

S

A

L

V

I

L

T

A

S
|
S

K
x
I

G
|
G

A
x
T

H

A

G
|
G

I
x
V

P

P

G

G

S
x
A

A
x
G

L

A

V

I

I

M

L

L

A

A

A

M

T

V

L

L

T

Q

A

S

I

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

I

S

P

P

V

V

-

A

V

L

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L
x
V

I

T

G

G

N
x
D

C

L

V

A

A

G

T

T

V

V

A

I

I

V

A

A

R

K

W

T

E

E

K

K

D

E

I

L

D

D

V

E

Q

S

R

K

binding aspartic acid: R274 (≠ A268), S275 (= S269), S276 (= S270), T313 (≠ F306), G353 (= G346), V354 (≠ I347), A357 (≠ S350), G358 (≠ A351), D394 (≠ G381), R397 (= R384), T398 (≠ A385)
binding decyl-beta-d-maltopyranoside: L194 (≠ R188), G198 (≠ M192), Y202 (≠ L196)
binding sodium ion: Y87 (≠ F81), T90 (≠ L84), S91 (≠ T85), S276 (= S270), G305 (= G298), A306 (≠ Y299), T307 (≠ S300), N309 (= N302), N309 (= N302), M310 (≠ L303), D311 (= D304), S348 (= S341), I349 (≠ K342), G350 (= G343), T351 (≠ A344), N401 (= N388), V402 (≠ L389), D405 (≠ N392)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
26% identity, 93% coverage: 3:410/437 of query aligns to 4:423/426 of 6xwnB

query
sites
6xwnB

R

R

W

Y

C

L

S

D

R

Y

S

P

I

V

F

L

L

W

Q

K

V

I

V

L

L

W

G

G

L

L

V

V

L

L

G

G

I

A

V

V

C

F

G

G

L

L

T

I

L

A

P

G

E

H

Y

F

-

G

-

Y

-

A

-

G

-

A

-

V

S

K

A

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

L

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F

Y

E

L

V

A

L

T

T

S

T

A

I

M

A

A

L

V

V

F

I

F

G

G

L

L

V

I

F

V

A

G

F

R

S

L

T

F

G

N

I

V

G

G

S

A

G

N

A

V

N

N

I

L

H

-

L

-

E

-

Q

-

L

-

S

-

A

G

A

S

D

G

M

T

G

G

D

K

L

A

A

I

E

E

R

-

S

-

Q

A

H

Q

M

P

H

P

T

S

T

L

T

V

Q

Q

F

T

L

L

M

L

D

N

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

A

A

S

F

L

A

A

D

K

N

G

N

E

I

V

L

L

Q

P

V

V

L

I

L

F

F

F

S

A

V

I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

R

L

V

I

F

N

D

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R

L

I

I

M

V

G

G

M

G

I

V

V

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

R

S

V

L

V

Q

G

H

P

L

L

G

A

S

K

L

V

V

V

G

G

L

A

F

V

Y

Y

L

T

T

G

C

L

I

F

A

L

F

Q

V

I

S

V

V

I

I

T

L

Y

G

F

V

I

V

L

M

L

R

K

A

V

S

F

G

G

L

I

R

D

M

P

W

I

P

K

L

F

L

I

K

R

Y

K

L

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

M

F

G

V

T

T

A

R

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

-

V

K

A

L

E

E

E

H

E

L

M

G

G

I

V

G

D

S

K

S

G

T

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

F
x
T

S

A

I

L

Y

Y

L

Q

T

G

L

V

A

T

I

V

V

L

F

F

I

V

A

A

N

N

A

A

T

I

G

G

T

H

P

P

L

L

A

T

M

L

T

G

D

Q

L

Q

L

L

T

V

I

V

L

V

L

L

V

T

S

A

L

V

I

L

T

A

S

S

K

I

G

G

A

T

H

A

G

G

I

V

P

P

G

G

S

A

A

G

L

A

V

I

I

M

L

L

A

A

A

M

T

V

L

L

T

Q

A

S

I

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

I

S

P

P

V

V

-

A

V

L

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A

T

L

M

T

V

N

N

L

V

I

T

G

G

N

D

C

L

V

A

A

G

T

T

V

V

A

I

I

V

A

A

R

K

W

T

E

E

K

K

D

E

I

L

D

D

V

E

Q

S

R

K

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S269), S276 (= S270), M310 (≠ L303), T313 (≠ F306), D394 (≠ G381), R397 (= R384)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
27% identity, 90% coverage: 12:403/437 of query aligns to 6:407/407 of 2nwwA

query
sites
2nwwA

Q

K

V

I

V

L

L

I

G

G

L

L

V

I

L

L

G

G

I

A

V

I

C

V

G

G

L

L

T

I

L

L

P

G

E

H

Y

Y

-

G

-

Y

-

A

-

H

-

A

-

V

S

H

A

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

F

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F

Y

E

L

V

L

L

T

T

S

T

A

I

F

A

A

L

V

V

T

I

L

G

G

L

I

V

I

F

M

A

A

F

R

S

L

T

-

G

-

I

F

G

N

S

P

G

G

A

A

N

G

I

I

H

H

L

L

E

A

Q

-

L

-

S

-

A

-

D

-

M

V

G

G

D

G

L

Q

A

Q

E

F

R

Q

S

P

Q

H

H

Q

M

A

H

P

T

P

T

L

T

V

Q

H

F

I

L

L

M

L

D

D

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

G

A

A

F

L

A

A

D

N

N

G

N

Q

I

V

L

L

Q

P

V

T

L

I

L

F

F

F

S

A

V

I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

D

L

A

I

I

N

N

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R

K

I

I

M

V

G

N

M

G

I

V

V

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

H

S

V

L

V

Q

G

H

E

L

L

G

A

S

K

L

V

V

T

G

A

L

A

F

V

Y

Y

L

V

T

G

C

L

I

T

A

L

F

Q

V

I

S

L

V

L

I

V

L

Y

G

F

V

V

V

L

M

L

R

K

A

I

S

Y

G

G

L

I

R

D

M

P

W

I

P

S

L

F

L

I

K

K

Y

H

L

A

R

K

E

D

E

A

L

M

L

L

I

T

V

A

M

F

G

V

T

T

A

R

S

S

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

K

V

L

A

E

K

H

E

L

M

G

G

I

I

G

S

S

E

S

G

T

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

F
x
T

S

A

I

L

Y

Y

L

Q

T

G

L

V

A

C

I

T

V

F

F

F

I

I

A

A

N

N

A

A

T

L

G

G

T

S

P

H

L

L

A

T

M

V

T

G

D

Q

L

Q

L

L

T

T

I

I

L

V

L

L

V

T

S

A

L

V

I

L

T

A

S

S

K

I

G

G

A

T

H

A

G

G

I

V

P

P

G

G

S
x
A

A
x
G

L

A

V

I

I

M

L

L

A

A

A

M

T

V

L

L

T

H

A

S

I

V

P

-

A

G

I

L

P

P

V

L

V

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L

V

I

T

G

G

N

D

C

L

V

T

A

G

T

T

V

A

A

I

I

V

A

A

R

K

W

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S270), T305 (≠ F306), A349 (≠ S350), G350 (≠ A351), D385 (≠ G381), R388 (= R384), T389 (≠ A385), N392 (= N388)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
27% identity, 92% coverage: 3:403/437 of query aligns to 6:416/419 of 6x15A

query
sites
6x15A

R

K

W
x
Y

C

I

S

E

R

Y

S

P

I

V

F

L

L

Q

Q

K

V

I

V

L

L

I

G

G

L

L

V

I

L

L

G

G

I

A

V

I

C

V

G

G

L

L

T

I

L

L

P

G

E

H

Y

Y

-

G

-

Y

-

A

-

H

-

A

-

V

S

H

A

T

Q

Y

L

V

K

K

P

P

L
x
F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

C

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

F

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D
x
P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F
x
Y

E

L

V
x
L

L
x
T

T
x
S

T

A

I
x
F

A

A

L

V

V

T

I

L

G

G

L

I

V

I

F

M

A

A

F

R

S

L

T

-

G

-

I

F

G

N

S

P

G

G

A

A

N

G

I

I

H

H

L

L

E

A

Q

-

L

-

S

-

A

-

D

-

M

V

G

G

D

G

L

Q

A

Q

E

F

R

Q

S

P

Q

H

H

Q

M

A

H

P

T

P

T
x
L

T

V

Q

H

F
x
I

L

L

M

L

D

D

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

G

A

A

F

L

A

A

D

N

N

G

N

Q

I

V

L

L

Q

P

V

T

L

I

L

F

F

F

S

A

V
x
I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

D

L

A

I

I

N

N

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R
x
K

I

I

M

V

G

N

M
x
G

I

V

V

M

R

Q

L

Y

A

A

P

P

I
|
I

G

G

V

V

F
|
F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

H

S

V

L

V

Q

G

H

E

L

L

G

A

S

K

L

V

V

T

G

A

L

A

F

V

Y

Y

L

V

T

G

C

L

I

T

A

L

F

Q

V

I

S

L

V

L

I

V

L

Y

G

F

V

V

V
x
L

M

L

R

K

A

I

S

Y

G

G

L

I

R

D

M

P

W

I

P

S

L

F

L
x
I

K

K

Y

H

L

A

R

K

E

D

E

A

L
x
M

L

L

I

T

V

A

M

F

G

V

T

T

A

R

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

K

V

L

A

E

K

H

E

L

M

G

G

I

I

G

S

S

E

S

G

T

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y

A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

F
x
T

S

A

I

L

Y

Y

L

Q

T

G

L

V

A

A

I

T

V

F

F

F

I

I

A

A

N

N

A

A

T

L

G

G

T

S

P

H

L

L

A
x
T

M
x
V

T
x
G

D

Q

L

Q

L

L

T
|
T

I

I

L

V

L
|
L

V

T

S

A

L

V

I

L

T

A

S
|
S

K
x
I

G

G

A
x
T

H

A

G
|
G

I

V

P

P

G

G

S
x
A

A
x
G

L

A

V

I

I

M

L

L

A

C

A

M

T

V

L

L

T

H

A

S

I

V

P

-

A

G

I

L

P

P

V

L

V

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A
x
T

L
x
M

T

V

N
|
N

L
x
V

I

T

G

G

N
x
D

C

L

V

T

A

G

T

T

V

A

A

I

I

V

A

A

R

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y7 (≠ W4), F46 (≠ L37), F46 (≠ L37), P75 (≠ D66), L91 (≠ V83), F95 (≠ I87), L130 (≠ T130), I133 (≠ F133), I159 (≠ V159), Y167 (≠ L167), K196 (≠ R188), G200 (≠ M192), I207 (= I199), F210 (= F202), L250 (≠ V242), I262 (≠ L254), M269 (≠ L261), T334 (≠ A326), V335 (≠ M327), G336 (≠ T328), T340 (= T332), L343 (= L335), M399 (≠ L386)
binding aspartic acid: S277 (= S269), S278 (= S270), T314 (≠ F306), G354 (= G346), A358 (≠ S350), G359 (≠ A351), D394 (≠ G381), R397 (= R384), T398 (≠ A385)
binding sodium ion: Y89 (≠ F81), T92 (≠ L84), S93 (≠ T85), G306 (= G298), T308 (≠ S300), N310 (= N302), N310 (= N302), M311 (≠ L303), D312 (= D304), S349 (= S341), I350 (≠ K342), T352 (≠ A344), N401 (= N388), V402 (≠ L389), D405 (≠ N392)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
27% identity, 92% coverage: 3:403/437 of query aligns to 3:413/413 of 6x14A

query
sites
6x14A

R

K

W
x
Y

C

I

S

E

R

Y

S

P

I

V

F

L

L

Q

Q

K

V
x
I

V

L

L

I

G

G

L

L

V

I

L

L

G

G

I

A

V

I

C

V

G

G

L

L

T

I

L

L

P

G

E

H

Y

Y

-

G

-

Y

-

A

-

H

-

A

-

V

S

H

A

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

C

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

F

C

A

V

S

V

L

V

V

S

V

G
|
G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V
|
V

I

V

Y

Y

F

Y

E

L

V

L

L

T

T

S

T

A

I

F

A

A

L

V

V

T

I

L

G

G

L

I

V

I

F

M

A

A

F

R

S

L

T

-

G

-

I

F

G

N

S

P

G

G

A

A

N

G

I

I

H

H

L

L

E

A

Q

-

L

-

S

-

A

-

D

-

M

V

G

G

D

G

L

Q

A

Q

E

F

R

Q

S

P

Q

H

H

Q

M

A

H

P

T

P

T

L

T

V

Q

H

F

I

L

L

M

L

D

D

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

G

A

A

F

L

A

A

D

N

N

G

N

Q

I

V

L

L

Q

P

V

T

L

I

L

F

F

F

S

A

V

I

L
x
I

F

L

G

G

S

I

A

A

L

I

N

T

L
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

D

L

A

I

I

N

N

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R
x
K

I

I

M

V

G

N

M

G

I

V

V
x
M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

H

S

V

L

V

Q

G

H

E

L

L

G

A

S

K

L

V

V

T

G

A

L

A

F

V

Y

Y

L

V

T

G

C

L

I

T

A

L

F

Q

V

I

S

L

V

L

I

V

L

Y

G

F

V

V

V

L

M

L

R

K

A

I

S

Y

G

G

L

I

R

D

M

P

W

I

P

S

L

F

L

I

K

K

Y

H

L

A

R

K

E

D

E

A

L

M

L

L

I

T

V

A

M

F

G

V

T

T

A

R

S

S

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

K

V

L

A

E

K

H

E

L

M

G

G

I

I

G

S

S

E

S

G

T

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S
x
T

F
x
I

N

N

L

M

D

D

G

G

F
x
T

S

A

I

L

Y

Y

L

Q

T

G

L

V

A

A

I

T

V

F

F

F

I

I

A

A

N

N

A

A

T

L

G

G

T

S

P

H

L

L

A

T

M

V

T

G

D

Q

L

Q

L

L

T

T

I

I

L

V

L

L

V

T

S

A

L

V

I

L

T

A

S

S

K
x
I

G

G

A

T

H

A

G

G

I

V

P

P

G

G

S

A

A
x
G

L

A

V

I

I

M

L

L

A

C

A

M

T

V

L

L

T

H

A

S

I

V

P

-

A

G

I

L

P

P

V

L

V

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

V

A

L

M

V

I

L
|
L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A

T

L

M

T

V

N

N

L

V

I

T

G

G

N

D

C

L

V

T

A

G

T

T

V

A

A

I

I

V

A

A

R

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ W4), G66 (= G60), V83 (= V78), I157 (≠ L160), Y164 (≠ L167), K193 (≠ R188), T305 (≠ S300), I306 (≠ F301), I347 (≠ K342)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V13), M199 (≠ V194), S275 (= S270), T311 (≠ F306), G356 (≠ A351), L384 (= L374), D391 (≠ G381), R394 (= R384)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
27% identity, 92% coverage: 9:410/437 of query aligns to 3:412/416 of 6r7rA

query
sites
6r7rA

I

V

F

L

L

W

Q

K

V

I

V

L

L

W

G

G

L

L

V

V

L

L

G

G

I

A

V

V

C

F

G

G

L

L

T

I

L

A

P

G

E
x
H

Y

F

-

G

-

Y

-

A

-

G

-

A

-

V

S

K

A

T

Q

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

L

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F

Y

E

L

V

A

L

T

T

S

T

A

I

M

A

A

L

V

V

F

I

F

G

G

L

L

V

I

F

V

A

G

F

R

S

L

T

F

G

N

I

V

G

G

S

-

G

-

A

A

N

N

I

V

H

N

L

L

E

G

Q

S

L

G

S

T

A

G

A

A

D

Q

M

P

G

P

D

S

L

L

A

-

E

-

R

-

S

-

Q

-

H

-

M

-

H

-

T

-

T

V

Q

Q

F

T

L

L

M

L

D

N

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

A

A

S

F

L

A

A

D

K

N

G

N

E

I

V

L

L

Q

P

V

V

L

I

L

F

F

F

S

A

V

I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

R

L

V

I

F

N

D

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R

L

I

I

M

V

G

G

M

G

I

V

V

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

R

S

V

L
x
V

Q

G

H

P

L

L

G
x
A

S

K

L

V

V

V

G

G

L

A

F

V

Y

Y

L

T

T

G

C

L

I

F

A

L

F

Q

V

I

S

V

V

I

I

T

L

Y

G

F

V

I

V

L

M

L

R

K

A

V

S

F

G

G

L

I

R

D

M

P

W

I

P

K

L

F

L

I

K

R

Y

K

L

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

M

F

G

V

T

T

A
x
R

S

S

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

-

V

K

A

L

E

E

E

H

E

L

M

G

G

I

V

G

D

S

K

S

G

T

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

F
x
T

S

A

I

L

Y

Y

L

Q

T

G

L

V

A

T

I

V

V

L

F

F

I

V

A

A

N

N

A

A

T

I

G

G

T

H

P

P

L

L

A

T

M

L

T

G

D

Q

L

Q

L

L

T

V

I

V

L

V

L

L

V

T

S

A

L

V

I

L

T

A

S

S

K

I

G

G

A
x
T

H

A

G
|
G

I
x
V

P

P

G

G

S

A

A
x
G

L

A

V

I

I

M

L

L

A

A

A

M

T

V

L

L

T

Q

A

S

I

V

-

G

-

L

-

D

-

L

-

T

P

P

A

G

I

S

P

P

V

V

-

A

V

L

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D

D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L

V

I

T

G

G

N

D

C

L

V

A

A

G

T

T

V

V

A

I

I

V

A

A

R

K

W

T

E

E

K

K

D

E

I

L

D

D

V

E

Q

S

R

K

binding d-aspartic acid: R263 (≠ A268), S265 (= S270), M299 (≠ L303), T302 (≠ F306), T340 (≠ A344), G342 (= G346), V343 (≠ I347), G347 (≠ A351), D383 (≠ G381), R386 (= R384), T387 (≠ A385), N390 (= N388)
binding decyl-beta-d-maltopyranoside: H23 (≠ E29), V212 (≠ L217), A216 (≠ G221)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
27% identity, 90% coverage: 12:403/437 of query aligns to 7:408/409 of 6bavA

query
sites
6bavA

Q

K

V

I

V

L

L

I

G

G

L

L

V

I

L

L

G

G

I

A

V

I

C

V

G

G

L

L

T

I

L

L

P

G

E

H

Y

Y

-

G

-

Y

-

A

-

D

-

A

-

V

S

K

A

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

F

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F

Y

E

L

V

L

L

T

T

S

T

A

I

F

A

A

L

V

V

T

I

L

G

G

L

I

V

I

F

M

A

A

F

R

S

L

T

-

G

-

I

F

G

N

S

P

G

G

A

A

N

G

I

I

H

H

L

L

E

A

Q

-

L

-

S

-

A

-

D

-

M

V

G

G

D

G

L

Q

A

Q

E

F

R

Q

S

P

Q

K

H

Q

M

A

H

P

T

P

T

L

T

V

Q

K

F

I

L

L

M

L

D

D

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

G

A

A

F

L

A

A

D

N

N

G

N

Q

I

V

L

L

Q

P

V

T

L

I

L

F

F

F

S

A

V

I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

D

L

A

I

I

N

N

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R

K

I

I

M

V

G

N

M

G

I

V

V

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

K

S

V

L

V

Q

G

H

E

L

L

G

A

S

K

L

V

V

T

G

A

L

A

F

V

Y

Y

L

V

T

G

C

L

I

T

A

L

F

Q

V

I

S

L

V

L

I

V

L

Y

G

F

V

V

V

L

M

L

R

K

A

I

S

Y

G

G

L

I

R

D

M

P

W

I

P

S

L

F

L

I

K

K

Y

K

L

A

R

K

E

D

E

A

L

M

L

L

I

T

V

A

M

F

G

V

T

T

A
x
R

S

S

S
|
S

D

S

A

G

V

T

L

L

P

P

Q

V

I

T

M

M

R

R

K

V

L

A

E

K

H

E

L

M

G

G

I

I

G

S

S

E

S

G

T

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

F
x
T

S

A

I

L

Y
|
Y

L

Q

T

G

L

V

A

C

I

T

V

F

F

F

I

I

A

A

N

N

A

A

T

L

G

G

T

S

P

H

L

L

A

T

M

V

T

G

D

Q

L

Q

L

L

T

T

I

I

L

V

L

L

V

T

S

A

L

V

I

L

T

A

S

S

K

I

G

G

A

T

H

A

G

G

I

V

P

P

G

G

S

A

A

G

L

A

V

I

I

M

L

L

A

A

A

M

T

V

L

L

T

E

A

S

I

V

P

-

A

G

I

L

P

P

V

L

V

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

V

A

L

M

V

I

L

L

A

G

V

I

D
|
D

W

A

F

I

M

L

G
x
D

I

M

G

G

R
x
C

A
x
T

L

M

T

V

N

N

L

V

I

T

G

G

N

D

C

L

V

T

A

G

T

T

V

A

A

I

I

V

A

A

R

K

W

T

E

E

binding benzylcysteine: R268 (≠ A268), S270 (= S270), T306 (≠ F306), Y309 (= Y309), D382 (= D377), D386 (≠ G381), C389 (≠ R384), T390 (≠ A385)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
27% identity, 90% coverage: 12:403/437 of query aligns to 7:408/408 of 6bauA

query
sites
6bauA

Q

K

V

I

V

L

L

I

G

G

L

L

V

I

L

L

G

G

I

A

V

I

C

V

G

G

L

L

T

I

L

L

P

G

E

H

Y

Y

-

G

-

Y

-

A

-

D

-

A

-

V

S

K

A

T

Q

Y

L

V

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

I

V

G

M

L

P

I

I

V

V

F

F

C

A

V

S

V

L

V

V

S

V

G

G

I

A

S

A

G

S

A

I

G

S

D

P

L

-

K

A

K

R

V

L

G

G

R

R

I

V

G

G

L

V

K

K

S

I

V

V

I

V

Y

Y

F

Y

E

L

V

L

L

T

T

S

T

A

I

F

A

A

L

V

V

T

I

L

G

G

L

I

V

I

F

M

A

A

F

R

S

L

T

-

G

-

I

F

G

N

S

P

G

G

A

A

N

G

I

I

H

H

L

L

E

A

Q

-

L

-

S

-

A

-

D

-

M

V

G

G

D

G

L

Q

A

Q

E

F

R

Q

S

P

Q

K

H

Q

M

A

H

P

T

P

T

L

T

V

Q

K

F

I

L

L

M

L

D

D

L

I

I

V

P

P

T

T

S

N

V

P

I

F

G

G

A

A

F

L

A

A

D

N

N

G

N

Q

I

V

L

L

Q

P

V

T

L

I

L

F

F

F

S

A

V

I

L

I

F

L

G

G

S

I

A

A

L

I

N

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

E

K

A

S

A

A

S

E

G

T

I

L

S

L

R

D

L

A

I

I

N

N

E

G

L

L

S

A

H

E

V

A

I

M

F

Y

R

K

I

I

M

V

G

N

M

G

I

V

V

M

R

Q

L

Y

A

A

P

P

I

I

G

G

V

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

T

M

S

A

K

E

Y

Q

G

G

L

V

D

K

S

V

L

V

Q

G

H

E

L

L

G

A

S

K

L

V

V

T

G

A

L

A

F

V

Y

Y

L

V

T

G

C

L

I

T

A

L

F

Q

V

I

S

L

V

L

I

V

L

Y

G

F

V

V

V

L

M

L

R

K

A

I

S

Y

G

G

L

I

R

D

M

P

W

I

P

S

L

F

L

I

K

K

Y

K

L

A

R

K