SitesBLAST

P46154 Glutathione-independent formaldehyde dehydrogenase; FALDH; FDH; Formaldehyde dismutase; EC 1.2.1.46; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
34% identity, 95% coverage: 2:388/407 of query aligns to 5:392/399 of P46154

query
sites
P46154

K

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A

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P

C47 (= C38) binding Zn(2+)
GSDQH 48:52 (≠ GSDLH 39:43) binding NAD(+)
H68 (= H60) binding Zn(2+)
C98 (= C90) binding Zn(2+)
C101 (= C93) binding Zn(2+)
C104 (= C96) binding Zn(2+)
C112 (= C104) binding Zn(2+)
V198 (= V197) binding NAD(+)
D218 (= D216) binding NAD(+)
R223 (= R221) binding NAD(+)
V263 (= V261) binding NAD(+)
RGH 268:270 (≠ HGL 266:268) binding NAD(+)
PGL 300:302 (≠ PGV 307:309) binding NAD(+)
GQT 337:339 (= GQT 332:334) binding NAD(+)

Q52078 Formaldehyde dismutase; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
32% identity, 95% coverage: 2:388/407 of query aligns to 5:392/399 of Q52078

query
sites
Q52078

K
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K

A
x
S

L
x
V

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x
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W
x
Y

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H

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-

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A
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A

K
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L
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L

G
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G

A
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A

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G

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N
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A
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E
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D

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Y
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Y

E
x
A

T
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F
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F

R

-

D

-

K
x
D

K
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K

D
x
G

D
x
S

C
x
P

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K
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K

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x
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V

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P

C46 (= C38) binding Zn(2+)
GSDQH 47:51 (≠ GSDLH 39:43) binding NAD(+)
H67 (= H60) binding Zn(2+)
C97 (= C90) binding Zn(2+)
C100 (= C93) binding Zn(2+)
C103 (= C96) binding Zn(2+)
C111 (= C104) binding Zn(2+)
D170 (= D169) binding Zn(2+)
T174 (= T173) binding NAD(+)
PV 197:198 (= PV 196:197) binding NAD(+)
DQ 218:219 (≠ DR 216:217) binding NAD(+)
R223 (= R221) binding NAD(+)
V263 (= V261) binding NAD(+)
H268 (= H266) binding NAD(+)
P299 (= P307) binding NAD(+)
PGI 299:301 (≠ PGV 307:309) binding NAD(+)
GMA 336:338 (≠ GQT 332:334) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2:399 modified: mature protein, Formaldehyde dismutase

2dphA Crystal structure of formaldehyde dismutase
32% identity, 95% coverage: 2:388/407 of query aligns to 4:391/398 of 2dphA

query
sites
2dphA

K

K

A

S

L

V

R

V

W

Y

H

H

G

G

T

T

G

R

D

D

V

L

S

R

V

V

D

E

T

T

V

V

D

P

D

Y

P

P

Q

K

I

L

-

E

-

H

-

N

-

R

-

K

L

L

E

E

P

H

T

A

D

-

C

V

I

I

I

L

E

K

T

V

L

S

T

T

A

N

I

I

C
|
C

G
|
G

S
|
S

D

D

L

Q

H
|
H

L

I

Y

Y

D

R

G

G

Y

R

I

F

P

-

T

I

M

V

E

P

D

K

G

G

D

H

I

V

L

L

G

G

H
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H

E
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E

F

I

M

T

G

G

R

E

V

V

E

E

V

K

G

G

S

S

E

D

V

V

Q

E

N

L

L

M

K

D

K

I

G

G

D

D

R

L

V

V

V

S

V

V

P

P

F
|
F

P

N

I

V

A

A

C
|
C

G

G

H

R

C
|
C

H

R

Y

N

C
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C

N

K

T

E

D

A

Q

R

Y

S

S

D

L

V

C
|
C

D

E

N

N

S

N

N
x
L

R

V

N

N

G

P

E

D

M

A

Q

D

A

L

E

G

A

A

Y

F

G

G

Y

F

A

D

A

-

G

-

I

-

F

-

G

-

Y

-

S

-

H

-

M

-

L

L

G

K

G

G

F

W

D

S

G

G

Q

Q

A

A

E

E

Y

Y

V

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R

L

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V

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P

Y

Y

A

A

D

D

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Y

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M

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L

M

L

K

K

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F

P

G

D

D

S

K

L

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T

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D

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E

E

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L

M

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E

-

K

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K

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D

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L

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T

F

L

L

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T
x
S

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D

I

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L

P
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P

T
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T

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Y

F

Q

H

A

G

A

C

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V

N

S

C

A

D

G

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E

K

E

P

G

G

D

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H

V

V

V

Y

V

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W
x
A

G
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G

S

A

G
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G

P
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P

V
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V

G

G

L

R

F

C

A

A

Q

A

I

A

S

G

A

A

K

R

M

L

L

L

G

G

A

A

N

A

V

C

I

V

G

I

I

V

-
x
G

D
|
D

R
x
Q

E

N

P

P

E

E

R
|
R

L

L

R

K

M

L

A

L

E

S

Q

D

F

-

A

A

G

G

S

F

A

E

T

T

I

I

N

D

F
x
L

E

R

D

N

-

S

-

A

-

P

-

L

E

R

D

D

V

Q

F

I

E

D

K

Q

L

I

K

L

G

G

L

K

N

P

D

E

G

-

R

-

G

-

P

V

D

D

A

C

C

G

I

V

D

D

A

A

V
|
V

G

G

L

F

E

E

A

A

H
|
H

G

G

L

L

G

G

L

D

T

E

G

A

A

N

A

T

Q

E

K

T

V

P

E

N

Q

G

T

A

L

L

K

-

L

-

Q

-

T

-

D

-

R

-

G

-

T

N

A

S

L

L

I

F

E

D

A

V

I

V

Q

R

S

A

C

-

G

-

K

-

G

G

T

A

V

I

S

G

I

I

P
|
P

G
|
G

V
x
I

Y

Y

G

V

G

G

V

S

-

D

-

P

-

D

-

P

I

V

N

N

-

K

-

D

-

A

-

G

-

S

-

G

-

R

-

L

H

H

F

L

N

D

I

F

G

G

A

K

A

M

F

W

G

T

K

K

G

S

L

I

T

R

F

I

K

M

M

T

G

G

Q

M

T
x
A

H

P

V

V

H

T

K

N

Y

Y

I

N

K

R

D

H

L

L

L

T

K

E

H

A

I

I

E

L

A

W

G

D

D

Q

L

M

D

P

-

Y

-

L

P

S

S

K

Y

V

I

M

I

N

T

I

H

E

K

V

A

I

P

T

L

L

A

D

K

Q

A

A

P

P

E

D

L

G

Y

Y

E

A

T

K

F

F

R

-

D

-

K

D

K

K

D

G

D

S

C

P

V

A

K
|
K

V

F

V

V

L

I

D

D

P

P

active site: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), H66 (= H60), E67 (= E61), C96 (= C90), C99 (= C93), C102 (= C96), C110 (= C104), L114 (≠ N108), S168 (≠ T168), D169 (= D169), P172 (= P172), K386 (= K383)
binding nicotinamide-adenine-dinucleotide: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), F92 (= F86), D169 (= D169), T173 (= T173), A192 (≠ W192), G193 (= G193), G195 (= G195), P196 (= P196), V197 (= V197), G216 (vs. gap), D217 (= D216), Q218 (≠ R217), R222 (= R221), L236 (≠ F236), V262 (= V261), H267 (= H266), P298 (= P307), G299 (= G308), I300 (≠ V309), A337 (≠ T334)
binding zinc ion: C45 (= C38), H66 (= H60), C96 (= C90), C99 (= C93), C102 (= C96), C110 (= C104), D169 (= D169)

7ux4A Crystallographic snapshots of ternary complexes of thermophilic secondary alcohol dehydrogenase from thermoanaerobacter pseudoethanolicus reveal the dynamics of ligand exchange and the proton relay network. (see paper)
28% identity, 89% coverage: 25:386/407 of query aligns to 22:347/350 of 7ux4A

query
sites
7ux4A

P

P

T

F

D

D

C

A

I

I

I

V

E

R

T

P

T

L

L

A

T

V

A

A

I

P

C
|
C

G

T

S
|
S

D

D

L

I

H

H

-

T

L

V

Y

F

D

E

G

G

Y

A

I

I

P

G

T

E

M

R

E

H

D

N

G

-

D

M

I

I

L

L

G

G

H
|
H

E
|
E

F

A

M

V

G

G

R

E

V

V

E

E

V

V

G

G

S

S

E

E

V

V

Q

K

N

D

L

F

K

K

K

P

G

G

D

D

R

R

V

V

P

P

F

-

P

-

I

-

A

-

C

-

G

A

H

A

C

T

H

P

Y

D

C

W

N

R

T

T

D

S

Q

E

Y

V

S

Q

L

R

C

G

D

Y

N

H

S

Q

N

H

R

S

N

G

G

G

E

M

M

L

Q

A

A

G

E
x
W

A

K

Y

F

G

S

Y

N

A

V

A

K

A

D

G

G

I

V

F

F

G

G

Y

-

S

-

H

-

M

-

L

-

G

-

F

-

D

-

G

-

Q

-

A

-

E

E

Y

F

V

F

R

H

V

V

P

N

Y

D

A

A

D

D

V

M

G

N

P

L

M

A

K

H

V

L

P

P

D

K

S

E

L

I

T

P

D

L

E

E

Q

A

V

A

L

V

F

M

L

I

T

P

D
|
D

I

M

Y

M

P

T

T
|
T

G

G

Y

F

Q

H

A

G

A

A

V

E

N

L

C

A

D

D

I

I

E

E

E

L

G

G

D

A

T

T

V

V

V

A

V

V

W

L

G
|
G

S
x
I

G
|
G

P
|
P

V
|
V

G

G

L

L

F

M

A

A

Q

V

I

A

S

G

A

A

K

K

M

L

L

R

G

G

A

A

-

G

N

R

V

I

I

I

G

A

I

V

D

G

R

S

E
x
R

P

P

E

V

R

C

L

V

R

D

M

A

A

A

E

K

Q

Y

F

Y

A

G

G

A

S

T

A

D

T

I

I

V

N

N

F

Y

E

K

D

D

E

G

D

P

V

I

F

E

E

S

K

Q

L

I

K

M

G

N

L

L

N

T

D

E

G

G

R

K

G

G

P

V

D

D

A

A

C

A

I

I

D

I

A

A

V

-

G

-

L

-

E

-

A

-

H

-

G

-

L

-

G

-

L

-

T

G

G
|
G

A

N

A

A

Q

D

K

I

V

M

E

A

Q

T

T

A

L

V

K

K

L

I

Q

V

T

K

D

P

R

G

G

G

T

T

A

-

L

-

I

I

E

A

A

N

I
x
V

Q
x
N

S
x
Y

C

F

G

G

K

E

G

G

G

E

T

V

V

L

S

P

I

V

P

P

G

R

V

L

-

E

Y

W

G

G

G

C

V

G

I

M

N

A

H

H

F

K

N

T

I

I

G

K

A

G

F

L

G
x
C

K

P

G

G

L

G

T

R

F

L

K

R

M

M

G

-

Q

-

T

-

H

-

V

-

H

-

K

-

Y

-

I

-

K

E

D

R

L

L

L

I

K

D

H

L

I

V

E

F

A

Y

G

K

D

R

L

V

D

D

P

P

S

S

Y

K

I

L

I

V

T

T

H

H

K

V

-

F

A

R

P

G

L

F

A

D

K

N

A

I

P

E

E

K

L

A

Y

F

E

M

T

L

F

M

R

K

D

D

K

K

K

P

D

K

D

D

C

L

V

I

K

K

V

P

V

V

L

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: D148 (= D169), T152 (= T173), G172 (= G193), I173 (≠ S194), G174 (= G195), P175 (= P196), V176 (= V197), R198 (≠ E218), G242 (= G272), V263 (≠ I295), N264 (≠ Q296), Y265 (≠ S297), C293 (≠ G324)
binding (1S,3S)-3-methylcyclohexan-1-ol: S37 (= S40), H57 (= H60), W108 (≠ E116), D148 (= D169)
binding zinc ion: C35 (= C38), H57 (= H60), E58 (= E61), D148 (= D169)

7uutA Ternary complex crystal structure of secondary alcohol dehydrogenases from the thermoanaerobacter ethanolicus mutants c295a and i86a provides better understanding of catalytic mechanism (see paper)
28% identity, 89% coverage: 25:386/407 of query aligns to 24:349/352 of 7uutA

query
sites
7uutA

P

P

T

F

D

D

C

A

I

I

I

V

E

R

T

P

T

L

L

A

T

V

A

A

I

P

C
|
C

G
x
T

S
|
S

D

D

L

I

H

H

-

T

L

V

Y

F

D

E

G

G

Y

A

I

I

P

G

T

E

M

R

E

H

D

N

G

-

D

M

I

I

L

L

G

G

H
|
H

E

E

F

A

M

V

G

G

R

E

V

V

E

E

V

V

G

G

S

S

E

E

V

V

Q

K

N

D

L

F

K

K

K

P

G

G

D

D

R

R

V

V

P

P

F

-

P

-

I

-

A

-

C

-

G
x
A

H

A

C

T

H

P

Y

D

C

W

N

R

T

T

D

S

Q

E

Y

V

S

Q

L

R

C

G

D

Y

N

H

S

Q

N

H

R

S

N

G

G

G

E

M

M

L

Q

A

A

G

E
x
W

A

K

Y

F

G

S

Y

N

A

V

A

K

A

D

G

G

I

V

F

F

G

G

Y

-

S

-

H

-

M

-

L

-

G

-

F

-

D

-

G

-

Q

-

A

-

E

E

Y

F

V

F

R

H

V

V

P

N

Y

D

A

A

D

D

V

M

G

N

P

L

M

A

K

H

V

L

P

P

D

K

S

E

L

I

T

P

D

L

E

E

Q

A

V

A

L

V

F

M

L

I

T

P

D
|
D

I

M

Y

M

P

T

T
|
T

G

G

Y

F

Q

H

A

G

A

A

V

E

N

L

C

A

D

D

I

I

E

E

E

L

G

G

D

A

T

T

V

V

V

A

V

V

W

L

G
|
G

S

I

G
|
G

P
|
P

V
|
V

G

G

L

L

F

M

A

A

Q

V

I

A

S

G

A

A

K

K

M

L

L

R

G

G

A

A

-

G

N

R

V

I

I

I

G

A

I

V

D

G

R
x
S

E
x
R

P

P

E

V

R

C

L

V

R

D

M

A

A

A

E

K

Q

Y

F

Y

A

G

G

A

S

T

A

D

T

I

I

V

N

N

F

Y

E

K

D

D

E

G

D

P

V

I

F

E

E

S

K

Q

L

I

K

M

G

N

L

L

N

T

D

E

G

G

R

K

G

G

P

V

D

D

A

A

C

A

I

I

D

I

A
|
A

V

-

G

-

L

-

E

-

A

-

H

-

G

-

L

-

G

-

L

-

T
x
G

G
|
G

A

N

A

A

Q

D

K
x
I

V

M

E

A

Q

T

T

A

L

V

K

K

L

I

Q

V

T

K

D

P

R

G

G

G

T

T

A

-

L

-

I

I

E

A

A

N

I
x
V

Q
x
N

S
x
Y

C

F

G

G

K

E

G

G

G

E

T

V

V

L

S

P

I

V

P

P

G

R

V

L

-

E

Y

W

G

G

G

C

V

G

I

M

N

A

H

H

F

K

N

T

I

I

G

K

A

G

F

L

G
x
C

K

P

G

G

L

G

T

R

F

L

K

R

M

M

G

-

Q

-

T

-

H

-

V

-

H

-

K

-

Y

-

I

-

K

E

D

R

L

L

L

I

K

D

H

L

I

V

E

F

A

Y

G

K

D

R

L

V

D

D

P

P

S

S

Y

K

I

L

I

V

T

T

H

H

K

V

-

F

A

R

P

G

L

F

A

D

K

N

A

I

P

E

E

K

L

A

Y

F

E

M

T

L

F

M

R

K

D

D

K
|
K

K

P

D

K

D

D

C

L

V

I

K

K

V

P

V

V

L

V

binding (2R)-pentan-2-ol: S39 (= S40), H59 (= H60), A85 (≠ G91), W110 (≠ E116), D150 (= D169), C295 (≠ G324)
binding nadp nicotinamide-adenine-dinucleotide phosphate: C37 (= C38), T38 (≠ G39), S39 (= S40), D150 (= D169), T154 (= T173), G174 (= G193), G176 (= G195), P177 (= P196), V178 (= V197), S199 (≠ R217), R200 (≠ E218), A242 (= A260), G243 (≠ T271), G244 (= G272), I248 (≠ K276), V265 (≠ I295), N266 (≠ Q296), Y267 (≠ S297), C295 (≠ G324), K340 (= K377)
binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D169)

1ykfA NADP-dependent alcohol dehydrogenase from thermoanaerobium brockii (see paper)
28% identity, 89% coverage: 25:386/407 of query aligns to 24:349/352 of 1ykfA

query
sites
1ykfA

P

P

T

F

D

D

C

A

I

I

I

V

E

R

T

P

T

L

L

A

T

V

A

A

I

P

C
|
C

G
x
T

S
|
S

D

D

L

I

H
|
H

-

T

L

V

Y

F

D

E

G

G

Y

A

I

I

P

G

T

E

M

R

E

H

D

N

G

-

D

M

I

I

L

L

G

G

H
|
H

E
|
E

F

A

M

V

G

G

R

E

V

V

E

E

V

V

G

G

S

S

E

E

V

V

Q

K

N

D

L

F

K

K

K

P

G

G

D

D

R

R

V

V

P

P

F

-

P

-

I

-

A

-

C

-

G

A

H

I

C

T

H

P

Y
x
D

C

W

N

R

T
|
T

D

S

Q

E

Y
x
V

S

Q

L

R

C

G

D

Y

N

H

S

Q

N

H

R
x
S

N

G

G

G

E

M

M

L

Q

A

A

G

E

W

A

K

Y

F

G

S

Y

N

A

V

A

K

A

D

G

G

I

V

F

F

G

G

Y

-

S

-

H

-

M

-

L

-

G

-

F

-

D

-

G

-

Q

-

A

-

E

E

Y

F

V

F

R

H

V

V

P

N

Y

D

A

A

D

D

V

M

G

N

P

L

M

A

K

H

V

L

P

P

D

K

S

E

L

I

T

P

D

L

E

E

Q

A

V

A

L

V

F

M

L

I

T

P

D
|
D

I

M

Y

M

P

T

T
|
T

G

G

Y

F

Q

H

A

G

A

A

V

E

N

L

C

A

D

D

I

I

E

E

E

L

G

G

D

A

T

T

V

V

V

A

V

V

W

L

G
|
G

S
x
I

G
|
G

P
|
P

V
|
V

G

G

L

L

F

M

A

A

Q

V

I

A

S

G

A

A

K

K

M

L

L

R

G

G

A

A

-

G

N

R

V

I

I

I

G

A

I

V

D

G

R
x
S

E
x
R

P

P

E

V

R

C

L

V

R

D

M

A

A

A

E

K

Q

Y

F

Y

A

G

G

A

S

T

A

D

T

I

I

V

N

N

F
x
Y

E

K

D

D

E

G

D

P

V
x
I

F

E

E

S

K

Q

L

I

K

M

G

N

L

L

N

T

D

E

G

G

R

K

G

G

P

V

D

D

A

A

C

A

I

I

D

I

A

A

V

-

G

-

L

-

E

-

A

-

H

-

G

-

L

-

G

-

L

-

T

G