SitesBLAST
Comparing WP_095509899.1 NCBI__GCF_002283365.1:WP_095509899.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3r3sA Structure of the ygha oxidoreductase from salmonella enterica
60% identity, 95% coverage: 12:299/302 of query aligns to 2:289/292 of 3r3sA
- active site: G58 (= G68), S184 (= S194), L194 (= L204), Y197 (= Y207), K201 (= K211), Q242 (= Q252)
- binding magnesium ion: D56 (= D66), S57 (= S67), E82 (= E92)
- binding nicotinamide-adenine-dinucleotide: D56 (= D66), S57 (= S67), G58 (= G68), I59 (= I69), L79 (= L89), E82 (= E92), D106 (= D116), L107 (= L117), V133 (≠ N143), A134 (= A144), G135 (= G145), S184 (= S194), Y197 (= Y207), K201 (= K211), P227 (= P237), G228 (= G238), I230 (≠ F240), T232 (= T242), L234 (= L244), Q235 (= Q245)
5jydB Crystal structure of a putative short chain dehydrogenase from burkholderia cenocepacia
59% identity, 95% coverage: 12:298/302 of query aligns to 2:288/292 of 5jydB
- active site: G58 (= G68), S184 (= S194), L194 (= L204), Y197 (= Y207), K201 (= K211), P242 (≠ Q252)
- binding magnesium ion: D56 (= D66), S57 (= S67), E82 (= E92)
- binding nicotinamide-adenine-dinucleotide: G54 (= G64), D56 (= D66), S57 (= S67), G58 (= G68), I59 (= I69), L79 (= L89), E82 (= E92), D106 (= D116), I107 (≠ L117), N133 (= N143), A134 (= A144), A135 (≠ G145), T182 (= T192), S184 (= S194), Y197 (= Y207), K201 (= K211), P227 (= P237), G228 (= G238), P229 (= P239), Y230 (≠ F240), T232 (= T242), L234 (= L244), Q235 (= Q245)
P0AG84 Uncharacterized oxidoreductase YghA; EC 1.-.-.- from Escherichia coli (strain K12) (see paper)
58% identity, 95% coverage: 12:299/302 of query aligns to 4:291/294 of P0AG84
- K39 (≠ E47) modified: N6-acetyllysine
3ijrF 2.05 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD+
46% identity, 91% coverage: 24:298/302 of query aligns to 12:285/290 of 3ijrF
- active site: G57 (= G68), S182 (= S194), L192 (= L204), Y195 (= Y207), K199 (= K211), K240 (≠ D253)
- binding magnesium ion: D55 (= D66), S56 (= S67), E80 (= E92)
- binding nicotinamide-adenine-dinucleotide: P21 (= P32), D55 (= D66), S56 (= S67), G57 (= G68), I58 (= I69), Y77 (= Y88), L78 (= L89), E80 (= E92), G103 (= G115), D104 (= D116), L105 (= L117), N131 (= N143), V132 (≠ A144), A133 (≠ G145), Q134 (≠ K146), I155 (≠ T167), T180 (= T192), S182 (= S194), Y195 (= Y207), K199 (= K211), P225 (= P237), G226 (= G238), P227 (= P239), I228 (≠ F240), T230 (= T242), L232 (= L244)
3i3oA 2.06 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD-acetone
46% identity, 91% coverage: 24:298/302 of query aligns to 4:277/282 of 3i3oA
- active site: G49 (= G68), S174 (= S194), L184 (= L204), Y187 (= Y207), K191 (= K211), K232 (≠ D253)
- binding magnesium ion: D47 (= D66), S48 (= S67), E72 (= E92)
- binding nicotinamide adenine dinucleotide acetone adduct: G45 (= G64), D47 (= D66), S48 (= S67), G49 (= G68), I50 (= I69), Y69 (= Y88), L70 (= L89), E72 (= E92), G95 (= G115), D96 (= D116), L97 (= L117), N123 (= N143), V124 (≠ A144), A125 (≠ G145), Q126 (≠ K146), Q127 (= Q147), I147 (≠ T167), T172 (= T192), S174 (= S194), Y187 (= Y207), K191 (= K211), P217 (= P237), G218 (= G238), I220 (≠ F240), T222 (= T242), L224 (= L244)
8bcjB Crystal structure of short-chain dehydrogenase pa3128 from pseudomonas aeruginosa pao1 in complex with NADP+
37% identity, 79% coverage: 61:298/302 of query aligns to 8:249/250 of 8bcjB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G64), S13 (≠ D66), R14 (≠ S67), G15 (= G68), I16 (= I69), L36 (= L89), R37 (≠ D90), N38 (≠ E92), A61 (≠ G115), D62 (= D116), V63 (≠ L117), N89 (= N143), A90 (= A144), G91 (= G145), T113 (= T167), V143 (≠ T192), S145 (= S194), Y159 (= Y207), K163 (= K211), P189 (= P237), G190 (= G238), I192 (≠ F240), T194 (= T242), I196 (≠ L244), H197 (≠ Q245)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 81% coverage: 54:298/302 of query aligns to 4:241/244 of 4nbuB
- active site: G18 (= G68), N111 (= N168), S139 (= S194), Q149 (≠ L204), Y152 (= Y207), K156 (= K211)
- binding acetoacetyl-coenzyme a: D93 (≠ Q150), K98 (≠ D155), S139 (= S194), N146 (≠ S201), V147 (≠ A202), Q149 (≠ L204), Y152 (= Y207), F184 (≠ W241), M189 (≠ P246), K200 (≠ E257)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G64), N17 (≠ S67), G18 (= G68), I19 (= I69), D38 (≠ Y88), F39 (≠ L89), V59 (≠ G115), D60 (= D116), V61 (≠ L117), N87 (= N143), A88 (= A144), G89 (= G145), I90 (≠ Q147), T137 (= T192), S139 (= S194), Y152 (= Y207), K156 (= K211), P182 (= P237), F184 (≠ W241), T185 (= T242), T187 (≠ L244), M189 (≠ P246)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
36% identity, 83% coverage: 47:298/302 of query aligns to 1:251/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G64), S20 (≠ D66), K21 (≠ S67), G22 (= G68), I23 (= I69), A43 (≠ L89), S44 (≠ D90), S45 (≠ E92), G68 (= G115), D69 (= D116), V70 (≠ L117), N96 (= N143), S97 (≠ A144), G98 (= G145), Y100 (≠ Q147), I144 (≠ T192), S146 (= S194), Y159 (= Y207), K163 (= K211), P189 (= P237), G190 (= G238), M191 (≠ P239), I192 (≠ F240), T194 (= T242), G196 (≠ L244), T197 (≠ Q245)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S194), Y159 (= Y207), M191 (≠ P239), I202 (≠ Q250)
4iqgD Crystal structure of bpro0239 oxidoreductase from polaromonas sp. Js666 in NADP bound form
35% identity, 79% coverage: 61:298/302 of query aligns to 6:247/248 of 4iqgD
- active site: G13 (= G68), N112 (= N168), S143 (= S194), Y154 (≠ L204), Y157 (= Y207), K161 (= K211)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G64), S11 (≠ D66), R12 (≠ S67), G13 (= G68), I14 (= I69), N32 (= N87), A34 (≠ L89), S35 (≠ D90), N36 (≠ V91), A59 (≠ G115), D60 (= D116), V61 (≠ L117), N87 (= N143), A88 (= A144), G89 (= G145), V141 (≠ T192), S143 (= S194), Y157 (= Y207), K161 (= K211), P187 (= P237), G188 (= G238), I190 (≠ F240), T192 (= T242), I194 (≠ L244), H195 (≠ Q245)
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
33% identity, 82% coverage: 51:298/302 of query aligns to 1:254/258 of 3ak4A
- active site: G18 (= G68), S141 (= S194), L151 (= L204), Y154 (= Y207), K158 (= K211), E199 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ S67), G18 (= G68), I19 (= I69), D38 (≠ Y88), L39 (= L89), V60 (≠ L112), D61 (= D116), V62 (≠ L117), N88 (= N143), A89 (= A144), G90 (= G145), T139 (= T192), S141 (= S194), Y154 (= Y207), K158 (= K211), G185 (= G238), V187 (≠ F240), T189 (= T242), M191 (≠ L244)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
35% identity, 81% coverage: 54:298/302 of query aligns to 2:236/239 of 4nbtA
- active site: G16 (= G68), S132 (= S194), Y145 (= Y207), K149 (= K211)
- binding nicotinamide-adenine-dinucleotide: G12 (= G64), K15 (≠ S67), G16 (= G68), L17 (≠ I69), D36 (vs. gap), L37 (≠ I86), L52 (= L112), N53 (≠ L113), V54 (≠ P114), N80 (= N143), A81 (= A144), G82 (= G145), I130 (≠ T192), S132 (= S194), Y145 (= Y207), K149 (= K211), P177 (= P237), G178 (= G238), I180 (≠ F240), T182 (= T242)
G9FRD7 7alpha-hydroxysteroid dehydrogenase; 7alpha-HSDH; NADP-dependent 7alpha-hydroxysteroid dehydrogenase; EC 1.1.1.- from Clostridium sardiniense (Clostridium absonum) (see 2 papers)
35% identity, 81% coverage: 54:298/302 of query aligns to 3:248/262 of G9FRD7
- SSTRGI 13:18 (≠ GGDSGI 64:69) binding NADP(+)
- R38 (≠ E92) binding NADP(+); mutation to D: Loss of catalytic activity.
- NA 63:64 (≠ DL 116:117) binding NADP(+)
- N90 (= N143) binding NADP(+)
- T145 (≠ S194) binding taurochenodeoxycholate
- Y158 (= Y207) binding NADP(+); binding taurochenodeoxycholate
- K162 (= K211) binding NADP(+)
- IGTRA 191:195 (≠ FWT-- 240:242) binding NADP(+)
Sites not aligning to the query:
- 261:262 mutation Missing: 5-fold reduction in catalytic efficiency.
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
33% identity, 80% coverage: 58:298/302 of query aligns to 5:243/246 of 3osuA
5epoA The three-dimensional structure of clostridium absonum 7alpha- hydroxysteroid dehydrogenase (see paper)
35% identity, 81% coverage: 54:298/302 of query aligns to 2:247/261 of 5epoA
- active site: G16 (= G68), T144 (≠ S194), I152 (≠ A202), Y157 (= Y207), K161 (= K211), R193 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S12 (≠ G64), T14 (≠ D66), R15 (≠ S67), G16 (= G68), I17 (= I69), R37 (≠ E92), F61 (≠ G115), N62 (≠ D116), N89 (= N143), Y90 (≠ A144), G91 (= G145), Y157 (= Y207), K161 (= K211), P187 (= P237), G188 (= G238), I190 (≠ F240), T192 (= T242), R193 (vs. gap), A194 (vs. gap), A195 (≠ P243)
- binding taurochenodeoxycholic acid: T93 (= T148), T144 (≠ S194), G146 (≠ Q196), R154 (≠ L204), Y157 (= Y207), G188 (= G238), N198 (≠ P246), M199 (≠ S247), F203 (≠ P251)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
34% identity, 80% coverage: 58:298/302 of query aligns to 3:252/256 of Q48436
- 6:33 (vs. 61:90, 47% identical) binding NAD(+)
- D59 (= D116) binding NAD(+)
- K156 (= K211) binding NAD(+)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
33% identity, 80% coverage: 58:298/302 of query aligns to 2:236/239 of 3sj7A