SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_095511499.1 NCBI__GCF_002283365.1:WP_095511499.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8rplB Amp-forming acetyl-coa synthetase from chloroflexota bacterium with bound acetyl amp (see paper)
36% identity, 96% coverage: 15:643/658 of query aligns to 9:630/630 of 8rplB

query
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8rplB

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binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate: G391 (= G402), E392 (≠ S403), P393 (= P404), T416 (≠ Y427), W417 (≠ S428), W418 (≠ G429), Q419 (≠ G430), T420 (= T431), D502 (= D515), R517 (= R530), K523 (= K536), R528 (= R541)
binding magnesium ion: V539 (≠ N552), H541 (≠ A554)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
40% identity, 100% coverage: 1:658/658 of query aligns to 1:651/651 of P9WQD1

query
sites
P9WQD1

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R

R

A

D

A

V

H

A

L

E

D

N

G

R

D

E

L

L

G

G

N

D

V

T

T

S

A

T

L

L

E

L

D

D

E

P

D

T

A

V

V

F

E

D

A

A

I

I

R

R

R

A

A

A

R

K

K617 (= K624) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
38% identity, 88% coverage: 81:658/658 of query aligns to 67:642/648 of Q89WV5

query
sites
Q89WV5

R

K

W

W

C

F

V

E

G

D

G

G

R

V

M

L

N

N

V

V

V

A

H

H

N

N

C

C

L

I

D

D

R

R

W

H

A

L

G

H

T

K

E

R

A

A

W

-

E

N

R

Q

D

T

A

A

I

I

R

I

Y

W

E

E

S

G

E

D

E

D

-

P

G

S

T

Q

T

S

R

R

S

H

L

I

T

T

Y

Y

L

K

E

E

L

L

H

H

A

D

E

E

V

V

E

C

A

R

C

M

A

A

A

N

G

I

L

L

R

R

A

T

H

R

G

N

L

V

G

K

A

K

G

G

D

D

A

R

V

V

G

T

L

I

Y

Y

L

L

P

P

M

M

T

I

P

P

E

E

I

A

V

A

V

Y

A

A

F

M

L

L

A

A

V

C

A

A

R

R

I

I

G

G

G

A

I

I

I

H

L

S

P

V

L

V

F

F

S

A

G

G

Y

F

G

S

A

P

E

D

A

S

V

L

A

A

T

Q

R

R

L

I

A

N

D

D

G

C

N

Q

A

S

K

K

A

V

L

I

V

I

V

T

A

A

D

D

G

E

A

G

P

L

R

R

R

G

G

G

R

K

A

K

V

V

P

P

M

L

K

K

A

A

I

N

A

V

D

D

E

A

A

A

L

L

A

A

E

K

A

A

P

D

S

G

V

V

R

D

H

W

V

V

F

V

V

V

V

V

D

K

R

R

M

T

G

G

D

G

E

K

L

I

I

D

G

M

T

N

P

P

R

T

T

-

G

-

G

-

R

R

D

D

I

L

P

W

W

Y

S

H

R

E

L

A

L

A

E

A

I

M

G

-

R

-

Q

V

S

T

T

T

A

E

T

C

G

P

C

V

A

E

D

H

T

M

A

H

A

A

E

E

D

D

P

P

V

L

L

F

L

I

I

L

Y

Y

T

T

S

S

G
|
G

T

S

T

T

G
|
G

T

Q

P

P

K
|
K

G

G

A

V

V

L

H

H

T

T

H

S

C

A

G

G

F

Y

P

L

I

V

K

Y

A

A

A

A

Q

M

D

T

M

H

R

Q

H

Y

P

V

M

F

D

D

V

Y

R

H

A

D

G

G

D

D

V

I

V

Y

W

W

W

C

M

T

S

A

D

D

M

V

G

G

W

W

M

V

M

T

G

G

-

H

P

S

W

Y

L

I

V

L

F

Y

G

G

A

P

L

L

L

A

N

N

G

G

A

A

T

T

M

T

V

L

L

M

F

F

D

E

G

G

A

V

P

P

D

N

F

Y

P

P

E

D

A

N

D

S

R

R

T

F

W

W

A

N

I

V

C

I

E

D

R

K

H

H

G

K

V

V

T

N

L

T

L

F

G

Y

L

T

S

A

P

P

T

T

L

A

I

I

R

R

A

A

L

L

M

M

P

Q

H

G

G

G

A

D

G

E

P

P

V

V

Q

K

A

K

H

T

D

S

L

R

S

A

A

S

L

L

R

R

A

L

V

L

G

G

S

S

T

V

G

G

S

E

P

P

W

I

D

N

P

P

E

E

S

A

W

W

R

E

W

W

L

Y

F

H

S

R

T

V

V

V

L

G

D

E

S

D

N

R

R

C

P

P

I

I

L

V

N

D

Y

T

S

W

G

W

G

Q

T

T

E
|
E

I

-

S

T

G

G

G

G

I

I

V

L

C

I

G

T

N

P

-

L

-

P

H

G

V

A

Q

T

S

K

L

L

K

K

P

P

T

G

G

S

F

A

S

T

G

Q

P

P

V

F

L

F

G

G

M

V

D

V

A

P

D

E

V

I

V

V

D

D

E

A

S

D

G

G

E

K

P

V

V

L

R

E

G

G

-

E

A

T

V

T

G

G

E

N

L

L

A

C

I

L

R

T

Q

R

P

A

W

W

I

P

G

G

M

M

T

M

R

R

G

T

F

V

W

Y

G

G

D

D

E

-

D

-

N

H

A

A

R

R

Y

F

H

E

A

Q

T

T

Y

Y

W

F

A

S

R

T

T

Y

P

K

G

G

L

K

W

Y

V

F

H

T

G

G

D

D

F

G

A

C

A

R

V

R

D

D

A

A

D

D

G

G

Q

Y

W

Y

F

W

L

I

L

T

G

G

R

R

S

V

D

D

D

D

T

V

I

I

K

N

V

V

A

S

G

G

K

H

R

R

L

M

G

G

P

T

A

A

E

E

V

V

E

E

S

S

A

A

L

L

N

V

A

A

A

H

P

E

E

K

V

V

L

S

E

E

S

A

A

A

A

V

V

V

G

G

V

F

P

P

D

H

D

D

V

I

K

K

G

G

Q

Q

A

G

V

I

V

Y

A

A

F

Y

A

V

V

T

L

L

A

M

A

A

G

G

A

V

T

Q

E

P

S

T

D

E

A

D

L

L

R

R

E

K

A

E

L

L

I

V

G

T

R

W

V

V

T

R

D

K

A

E

L

I

G

G

K

P

A

I

L

A

R

S

P

P

K

D

A

Q

V

I

L

Q

F

F

V

A

D

P

A

G

L

L

P

P

K

K

T

T

R

R

N

S

A

G

K

K

V

I

M

M

R

R

R

R

V

I

I

L

R

R

A

K

A

I

H

A

L

E

D

D

-

E

-

P

G

G

D

S

L

L

G

G

N

D

V

T

T

S

A

T

L

L

E

A

D

D

E

P

D

A

A

V

V

V

E

D

A

D

I

L

R

V

R

K

A

N

R

R

G263 (= G282) mutation to I: Loss of activity.
G266 (= G285) mutation to I: Great decrease in activity.
K269 (= K288) mutation to G: Great decrease in activity.
E414 (= E432) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
35% identity, 95% coverage: 37:658/658 of query aligns to 24:645/652 of P27550

query
sites
P27550

Y

Y

A

E

A

A

L

M

E

Y

R

Q

W

Q

A

S

L

I

D

N

D

V

I

P

G

D

R

T

F

F

W

W

D

G

L

E

V

Q

M

G

E

K

D

I

L

L

G

-

I

-

E

D

F

W

S

I

Q

K

P

P

Y

Y

T

Q

R

K

A

V

L

K

D

N

A

T

S

S

-

F

-

A

G

P

G

G

I

N

E

V

R

S

P

I

R

K

W

W

C

Y

V

E

G

D

G

G

R

T

M

L

N

N

V

L

V

A

H

A

N

N

C

C

L

L

D

D

R

R

W

H

A

L

G

-

T

Q

E

E

A

N

W

G

E

D

R

R

D

T

A

A

I

I

R

I

Y

W

E

E

S

G

E

D

E

D

G

A

T

S

-

Q

T

S

R

K

S

H

L

I

T

S

Y

Y

L

K

E

E

L

L

H

H

A

R

E

D

V

V

E

C

A

R

C

F

A

A

A

N

G

T

L

L

R

L

A

E

H

L

G

G

L

I

G

K

A

K

G

G

D

D

A

V

V

V

G

A

L

I

Y

Y

L

M

P

P

M

M

T

V

P

P

E

E

I

A

V

A

V

V

A

A

F

M

L

L

A

A

V

C

A

A

R

R

I

I

G

G

G

A

I

V

I

H

L

S

P

V

L

I

F

F

S

G

G

G

Y

F

G

S

A

P

E

E

A

A

V

V

A

A

T

G

R

R

L

I

A

I

D

D

G

S

N

N

A

S

K

R

A

L

L

V

V

I

V

T

A

S

D

D

G

E

A

G

P

V

R

R

R

A

G

G

R

R

A

S

V

I

P

P

M

L

K

K

A

K

I

N

A

V

D

D

E

D

A

A

L

L

A

K

E

N

-

P

-

N

A

V

P

T

S

S

V

V

R

E

H

H

V

V

F

V

V

V

V

L

D

K

R

R

M

T

G

G

D

G

E

K

L

I

I

-

G

-

T

-

P

D

R

W

T

Q

G

E

G

G

R

R

D

D

I

L

P

W

W

W

S

H

R

D

L

L

L

V

E

E

I

-

G

-

R

Q

Q

A

S

S

T

D

A

Q

T

H

G

Q

C

A

A

E

D

E

T

M

A

N

A

A

E

E

D

D

P

P

V

L

L

F

L

I

I

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

T

K

P

P

K

K

G

G

A

V

V

L

H

H

T

T

H

T

C

G

G

G

F

Y

P

L

I

V

K

Y

A

A

A

A

Q

L

D

T

M

F

R

K

H

Y

P

V

M

F

D

D

V

Y

R

H

A

P

G

G

D

D

V

I

V

Y

W

W

W

C

M

T

S

A

D

D

M

V

G

G

W

W

M

V

M

T

G

G

-

H

P

S

W

Y

L

L

V

L

F

Y

G

G

A

P

L

L

L

A

N

C

G

G

A

A

T

T

M

T

V

L

L

M

F

F

D

E

G

G

A

V

P

P

D

N

F

W

P

P

E

T

A

P

D

A

R

R

T

M

W

A

A

Q

I

V

C

V

E

D

R

K

H

H

G

Q

V

V

T

N

L

I

L

L

G

Y

L

T

S

A

P

P

T

T

L

A

I

I

R

R

A

A

L

L

M

M

P

A

H

E

G

G

A

D

G

K

P

A

V

I

Q

E

A

G

H

T

D

D

L

R

S

S

A

S

L

L

R

R

A

I

V

L

G

G

S

S

T

V

G

G

S

E

P

P

W

I

D

N

P

P

E

E

S

A

W

W

R

E

W

W

L

Y

F

W

S

K

T

K

V

I

L

G

D

N

S

E

N

K

R

C

P

P

I

V

L

V

N

D

Y

T

S

W

G

W

G

Q

T

T

E

E

I

T

S

G

G

G

G

F

I

M

V

I

C

T

G

P

-

L

N

P

H

G

V

A

Q

T

S

E

L

L

K

K

P

A

T

G

G

S

F

A

S

T

G

R

P

P

V

F

L

F

G

G

M

V

D

Q

A

P

D

A

V

L

V

V

D

D

E

N

S

E

G

G

E

N

P

P

V

L

R

E

G

G

A

A

V

T

-

E

G

G

E

S

L

L

A

V

I

I

R

T

Q

D

P

S

W

W

I

P

G

G

M

Q

T

A

R

R

G

T

F

L

W

F

G

G

D

D

E

H

D

E

N

-

A

-

R

R

Y

F

H

E

A

Q

T

T

Y

Y

W

F

A

S

R

T

T

F

P

K

G

N

L

M

W

Y

V

F

H

S

G

G

D

D

F

G

A

A

A

R

V

R

D

D

A

E

D

D

G

G

Q

Y

W

Y

F

W

L

I

L

T

G

G

R

R

S

V

D

D

D

D

T

V

I

L

K

N

V

V

A

S

G

G

K

H

R

R

L

L

G

G

P

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

N

V

A

A

A

H

P

P

E

K

V

I

L

A

E

E

S

A

A

A

A

V

V

V

G

G

V

I

P

P

D

H

D

N

V

I

K

K

G

G

Q

Q

A

A

V

I

V

Y

A

A

F

Y

A

V

V

T

L

L

A

N

A

H

G

G

A

E

T

E

E

P

S

S

D

P

A

E

L

L

R

Y

E

A

A

E

L

V

I

R

G

N

R

W

V

V

T

R

D

K

A

E

L

I

G

G

K

P

A

L

L

A

R

T

P

P

K

D

A

V

V

L

L

H

F

W

V

T

D

D

A

S

L

L

P

P

K

K

T

T

R

R

N

S

A

G

K
|
K

V

I

M

M

R

R

R

R

V

I

I

L

R

R

-

K

-

I

A

A

A

A

H

G

L

D

D

T

G

S

D

N

L

L

G

G

N

D

V

T

T

S

A

T

L

L

E

A

D

D

E

P

D

G

A

V

V

V

E

E

A

K

I

L

R

L

R

E

A

E

R

K

K609 (= K624) modified: N6-acetyllysine; by autocatalysis

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
35% identity, 95% coverage: 35:658/658 of query aligns to 18:638/640 of 5jrhA

query
sites
5jrhA

D

E

G

Q

Y

Y

A

E

A

T

L

K

E

Y

R

K

W

Q

A

S

L

I

D

N

D

D

I

P

G

D

R

T

F

F

W

W

D

G

L

E

V

Q

M

G

E

K

D

I

L

L

G

-

I

-

E

D

F

W

S

I

Q

T

P

P

Y

Y

T

Q

R

K

A

V

L

K

D

N

A

T

S

S

-

F

-

A

G

P

G

G

I

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active site: T260 (= T280), T412 (= T431), E413 (= E432), N517 (≠ K536), R522 (= R541), K605 (= K624)
binding (r,r)-2,3-butanediol: W93 (≠ Y111), E140 (= E157), G169 (≠ T186), K266 (≠ T286), P267 (= P287)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G402), E384 (≠ S403), P385 (= P404), T408 (≠ Y427), W409 (≠ S428), W410 (≠ G429), Q411 (≠ G430), T412 (= T431), D496 (= D515), I508 (≠ L527), N517 (≠ K536), R522 (= R541)
binding coenzyme a: F159 (= F176), G160 (≠ S177), G161 (= G178), R187 (= R204), S519 (≠ A538), R580 (≠ T599), P585 (≠ K604)
binding magnesium ion: V533 (≠ N552), H535 (≠ A554), I538 (≠ V557)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
35% identity, 95% coverage: 35:658/658 of query aligns to 22:645/652 of Q8ZKF6

query
sites
Q8ZKF6

D

E

G

Q

Y

Y

A

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A

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x
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|
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|
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A

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A

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K

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A

A

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L

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A

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A

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x
R

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A

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A

L

L

A

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P

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D

A

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V

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L

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F

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T

D

D

A

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L

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P

K

K

T

T

R

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N

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A

G

K
|
K

V

I

M

M

R

R

R

R

V

I

I

L

R

R

-

K

-

I

A

A

A

A

H

G

L

D

D

T

G

S

D

N

L

L

G

G

N

D

V

T

T

S

A

T

L

L

E

A

D

D

E

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D

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A

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E

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A

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L

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E

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E

R

K

R194 (= R207) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (≠ M327) binding CoA
N335 (≠ D350) binding CoA
A357 (≠ S372) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D532) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (≠ A538) binding CoA
G524 (= G539) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R541) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ T599) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K624) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
34% identity, 95% coverage: 35:658/658 of query aligns to 18:639/641 of 2p20A

query
sites
2p20A

D

E

G

Q

Y

Y

A

E

A

T

L

K

E

Y

R

K

W

Q

A

S

L

I

D

N

D

D

I

P

G

D

R

T

F

F

W

W

D

G

L

E

V

Q

M

G

E

K

D

I

L

L

G

-

I

-

E

D

F

W

S

I

Q

T

P

P

Y

Y

T

Q

R

K

A

V

L

K

D

N

A

T

S

S

-

F

-

A

G

P

G

G

I

N

E

V

R

S

P

I

R

K

W

W

C

Y

V

E

G

D

G

G

R

T

M

L

N

N

V

L

V

A

H

A

N

N

C

C

L

L

D

D

R

R

W

H

A

L

G

-

T

Q

E

E

A

N

W

G

E

D

R

R

D

T

A

A

I

I

R

I

Y

W

E

E

S

G

E

D

E

D

G

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T

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-

Q

T

S

R

K

S

H

L

I

T

S

Y

Y

L

R

E

E

L

L

H

H

A

R

E

D

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V

E

C

A

R

C

F

A

A

A

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G

T

L

L

R

L

A

D

H

L

G

G

L

I

G

K

A

K

G

G

D

D

A

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G

A

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Y

L

M

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E

E

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A

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A

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A

A

F

M

L

L

A

A

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C

A

A

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R

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G

G

G

A

I

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H

L

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L

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G

G

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A

A

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A

A

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G

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L

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A

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A

G

G

R

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L

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K

A

K

I

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A

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D

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D

A

A

L

L

A

K

E

N

-

P

-

N

A

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S

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E

H

H

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F

I

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L

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K

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G

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D

L

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G

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L

L

L

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E

I

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G

A

P

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L

L

A

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C

G

G

A

A

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M

T

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L

L

M

F

F

D

E

G

G

A

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D

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F

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L

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G

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x
E

P
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P

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P

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A

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W

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W

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G

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x
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x
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x
W

G
x
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T

E
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E

I

T

S

G

G

G

G

F

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L

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F

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A

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G

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G

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A

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-

E

G

G

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A

R

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L

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G

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D

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E

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-

A

-

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F

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F

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R

T

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D

F

G

A

A

A

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V

R

D

D

A

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D

D

G

G

Q

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Y

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x
I

L

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G

G

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R

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D

D

D

D

T

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x
N

V

V

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G

G

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|
R

L

L

G

G

P

T

A

A

E

E

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I

E

E

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S

A

A

L

L

N

V

A

A

A

H

P

P

E

K

V

I

L

A

E

E

S

A

A

A

A

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G

V

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P

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H

D

A

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K

G

G

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Q

A

A

V

I

V

Y

A

A

F

Y

A

V

V

T

L

L

A

N

A

H

G

G

A

E

T

E

E

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S

S

D

P

A

E

L

L

R

Y

E

A

A

E

L

V

I

R

G

N

R

W

V

V

T

A

D

K

A

E

L

I

G

G

K

P

A

L

L

A

R

T

P

P

K

D

A

V

V

L

L

H

F

W

V

T

D

D

A

S

L

L

P

P

K

K

T

T

R

R

N

S

A

G

K
|
K

V

I

M

M

R

R

R

R

V

I

I

L

R

R

A

K

A

I

H

A

L

A

D

G

G

D

D

T

L

S

G

N

N

D

V

T

T

S

A

T

L

L

E

A

D

D

E

P

D

G

A

V

V

V

E

E

A

K

I

L

R

L

R

E

A

E

R

K

active site: T260 (= T280), T412 (= T431), E413 (= E432), N517 (≠ K536), R522 (= R541), K605 (= K624)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G402), E384 (≠ S403), P385 (= P404), T408 (≠ Y427), W409 (≠ S428), W410 (≠ G429), Q411 (≠ G430), T412 (= T431), D496 (= D515), I508 (≠ L527), R511 (= R530), R522 (= R541)

8rwjD Cryoem structure of acs1 filament determined by filamentid (see paper)
35% identity, 93% coverage: 33:647/658 of query aligns to 35:663/676 of 8rwjD

query
sites
8rwjD

G

G

L

L

D

Q

G

D

Y

Y

A

Q

A

R

L

L

E

H

R

K

W

E

A

S

L

I

D

E

D

D

I

P

G

A

R

K

F

F

W

F

D

G

L

S

V

K

M

A

E

T

D

Q

L

F

G

-

I

L

E

N

F

W

S

S

Q

K

P

P

Y

F

T

D

R

K

A

V

L

F

D

I

A

P

S

D

G

S

G

K

I

T

E

G

R

R

P

P

R

S

-

F

-

Q

-

N

-

N

-

A

W

W

C

F

V

L

G

N

G

G

R

Q

M

L

N

N

V

A

V

C

H

Y

N

N

C

C

L

V

D

D

R

R

W

H

A

A

G

-

T

L

E

K

A

T

W

P

E

N

R

K

D

K

A

A

I

I

R

I

Y

F

E

E

S

G

E

D

E

E

-

P

G

G

T

Q

T

G

R

Y

S

S

L

I

T

T

Y

Y

L

K

E

E

L

L

H

L

A

E

E

E

V

V

E

C

A

Q

C

V

A

A

A

Q

G

V

L

L

-

T

R

Y

A

S

H

M

G

G

L

V

G

R

A

K

G

G

D

D

A

T

V

V

G

A

L

V

Y

Y

L

M

P

P

M

M

T

V

P

P

E

E

I

A

V

I

V

I

A

T

F

L

L

L

A

A

V

I

A

S

R

R

I

I

G

G

G

A

I

I

I

H

L

S

P

V

L

V

F

F

S

A

G

G

Y

F

G

S

A

S

E

N

A

S

V

L

A

R

T

D

R

R

L

I

A

N

D

D

G

G

N

D

A

S

K

K

A

V

L

V

V

I

V

T

A

T

D

D

G

E

A

S

P

N

R

R

R

G

G

G

R

K

A

V

V

I

P

E

M

T

K

K

A

R

I

I

A

V

D

D

E

D

A

A

L

L

A

R

E

E

A

T

P

P

S

G

V

V

R

R

H

H

V

V

F

L

V

V

V

Y

D

R