SitesBLAST
Comparing WP_097029409.1 NCBI__GCF_900207575.1:WP_097029409.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4hcxA Structure of icdh-1 from m.Tuberculosis complexed with NADPH & mn2+ (see paper)
71% identity, 99% coverage: 3:400/404 of query aligns to 2:401/402 of 4hcxA
- binding manganese (ii) ion: D274 (= D273), D278 (= D277)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A73 (= A74), T74 (= T75), T76 (= T77), E305 (= E304), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), R313 (= R312), H314 (= H313), N327 (= N326)
P33198 Isocitrate dehydrogenase [NADP], mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Sus scrofa (Pig) (see 2 papers)
65% identity, 99% coverage: 3:403/404 of query aligns to 12:419/421 of P33198
- T86 (= T77) binding D-threo-isocitrate
- 103:109 (vs. 94:100, 100% identical) binding D-threo-isocitrate
- R118 (= R109) binding D-threo-isocitrate
- R141 (= R132) binding D-threo-isocitrate
- Y148 (= Y139) Critical for catalysis; mutation to F: Large decrease in Vmax and insignificant change in KM for isocitrate and NADP.
- K220 (= K210) Critical for catalysis; mutation K->Q,Y: Large decrease in Vmax and insignificant change in KM for isocitrate and NADP.
- D260 (= D250) binding Mn(2+)
- D283 (= D273) binding Mn(2+)
5h3eB Crystal structure of mouse isocitrate dehydrogenases 2 k256q mutant complexed with isocitrate (see paper)
66% identity, 99% coverage: 3:403/404 of query aligns to 2:409/410 of 5h3eB
6ajcA Crystal structure of trypanosoma cruzi cytosolic isocitrate dehydrogenase in complex with NADP+, isocitrate and ca2+
66% identity, 99% coverage: 3:401/404 of query aligns to 3:407/413 of 6ajcA
- binding calcium ion: D274 (= D273), D278 (= D277)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T75 (= T75), I76 (= I76), T77 (= T77), N96 (= N96), D252 (= D251), K259 (= K258), L287 (= L286), A307 (= A306), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), R313 (= R312), H314 (= H313), N327 (= N326)
P48735 Isocitrate dehydrogenase [NADP], mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Homo sapiens (Human) (see 4 papers)
66% identity, 99% coverage: 3:403/404 of query aligns to 43:450/452 of P48735
- P158 (= P118) to L: in GLM; somatic mutation; dbSNP:rs2151549649
- P162 (= P122) to S: in GLM; somatic mutation; dbSNP:rs1382680247
- R172 (= R132) to G: in GLM; somatic mutation; reduces enzymatic activity; dbSNP:rs1057519906; to K: in GLM; somatic mutation; reduces enzymatic activity; dbSNP:rs121913503; to M: in GLM; somatic mutation; reduces enzymatic activity; dbSNP:rs121913503; to S: found in patients with cartilagenous tumors; dbSNP:rs1057519736; to T: found in patients with cartilagenous tumors; dbSNP:rs121913503; to W: found in patients with cartilagenous tumors; dbSNP:rs1057519906
- K413 (= K372) modified: N6-acetyllysine; mutation to A: 44-fold loss in activity.; mutation to Q: 20-fold decrease in Vmax.; mutation to R: No appreciable difference in Km for isocitrate and NADP.
4ja8A Complex of mitochondrial isocitrate dehydrogenase r140q mutant with agi-6780 inhibitor (see paper)
65% identity, 99% coverage: 3:403/404 of query aligns to 3:410/416 of 4ja8A
- binding 1-[5-(cyclopropylsulfamoyl)-2-thiophen-3-yl-phenyl]-3-[3-(trifluoromethyl)phenyl]urea: W124 (= W124), V257 (≠ A256), Y271 (= Y270), D272 (= D271), V275 (= V274), Q276 (= Q275), I279 (≠ T278)
- binding calcium ion: D274 (= D273), D278 (= D277)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T75 (= T75), T77 (= T77), N96 (= N96), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), T312 (= T311), R313 (= R312), H314 (= H313), N327 (= N326)
5svoA Structure of idh2 mutant r140q (see paper)
65% identity, 99% coverage: 3:403/404 of query aligns to 2:409/410 of 5svoA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T74 (= T75), T76 (= T77), H307 (= H307), G308 (= G308), T309 (= T309), V310 (= V310), T311 (= T311), R312 (= R312), H313 (= H313), T325 (= T325), N326 (= N326)
5i95A Crystal structure of human mitochondrial isocitrate dehydrogenase r140q mutant homodimer bound to NADPH and alpha-ketoglutaric acid (see paper)
65% identity, 99% coverage: 3:403/404 of query aligns to 3:410/413 of 5i95A
- binding 2-oxoglutaric acid: T77 (= T77), S94 (= S94), N96 (= N96), R109 (= R109), D274 (= D273)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T75 (= T75), I76 (= I76), T77 (= T77), N96 (= N96), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), T312 (= T311), R313 (= R312), H314 (= H313), N327 (= N326)
5i96A Crystal structure of human mitochondrial isocitrate dehydrogenase (idh2) r140q mutant homodimer in complex with ag-221 (enasidenib) inhibitor. (see paper)
65% identity, 99% coverage: 3:403/404 of query aligns to 3:410/417 of 5i96A
- binding 2-methyl-1-[(4-[6-(trifluoromethyl)pyridin-2-yl]-6-{[2-(trifluoromethyl)pyridin-4-yl]amino}-1,3,5-triazin-2-yl)amino]propan-2-ol: Y271 (= Y270), D272 (= D271), V275 (= V274), Q276 (= Q275), I279 (≠ T278)
- binding calcium ion: D274 (= D273), S277 (= S276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T75 (= T75), T77 (= T77), E305 (= E304), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), T312 (= T311), R313 (= R312), H314 (= H313), N327 (= N326)
6adiA Crystal structures of idh2 r140q in complex with ag-881 (see paper)
65% identity, 99% coverage: 3:403/404 of query aligns to 3:410/418 of 6adiA
- binding 6-(6-chloropyridin-2-yl)-N2,N4-bis[(2R)-1,1,1-trifluoropropan-2-yl]-1,3,5-triazine-2,4-diamine: V254 (= V253), V257 (≠ A256), D272 (= D271), V275 (= V274), Q276 (= Q275), I279 (≠ T278)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T75 (= T75), I76 (= I76), T77 (= T77), N96 (= N96), E305 (= E304), H308 (= H307), G309 (= G308), T310 (= T309), V311 (= V310), H314 (= H313), T326 (= T325), N327 (= N326)
O88844 Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; IDH1; Cytosolic NADP-isocitrate dehydrogenase; IDPc; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Mus musculus (Mouse) (see paper)
65% identity, 100% coverage: 1:403/404 of query aligns to 1:410/414 of O88844