SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_097030475.1 NCBI__GCF_900207575.1:WP_097030475.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 92% coverage: 26:535/553 of query aligns to 24:531/541 of Q5SKN9

query
sites
Q5SKN9

L

L

K

E

R

R

A

A

E

A

V

L

F

F

T

G

D

R

R

K

-

E

-

V

I

V

A

S

L

R

V

L

H

H

G

T

A

G

T

E

-

V

-

H

R

R

L

T

T

T

Y

Y

G

A

E

E

Y

V

H

Y

A

Q

R

R

V

A

S

R

R

R

L

L

A

M

S

G

A

G

L

L

A

R

G

A

M

L

G

G

I

V

Q

G

P

V

G

G

D

D

V

R

V

V

A

A

T

T

I

L

L

G

P

F

N

N

I

H

A

F

Q

R

Q

H

A

L

E

E

A

A

H

Y

W

F

G

A

V

V

P

P

A

G

A

M

G

G

A

A

I

V

L

L

N

H

T

T

I

A

N

N

I

P

R

R

L

L

D

S

V

P

E

K

T

E

V

I

A

A

Y

Y

I

I

F

L

D

N

H

H

G

A

G

E

A

D

K

K

L

V

V

L

L

L

C

F

D

D

T

P

A

N

F

L

L

L

P

P

L

L

A

-

R

-

A

-

C

V

E

E

K

A

M

I

E

R

G

G

K

E

P

L

P

K

T

T

I

V

-

Q

-

H

I

F

E

V

V

V

C

M

D

D

R

E

E

K

A

A

G

P

H

E

S

G

A

-

T

-

G

-

E

-

F

Y

L

L

E

A

Y

Y

E

E

D

E

L

A

L

L

A

-

K

-

G

G

S

E

P

E

W

A

A

D

P

P

W

V

V

R

M

V

P

P

E

E

D

R

E

A

W

-

E

-

S

A

L

C

A

G

L

M

N

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

R

L

P

P

K

K

G

G

V

V

Y

Y

H

S

H

H

R

R

G

A

A

L

Y

V

L

L

A

H

T

S

Y

L

G

A

N

A

A

S

I

L

A

V

W

D

R

G

M

T

Q

A

L

L

Y

S

P

E

-

K

-

D

V

V

Y

V

L

L

T

P

I

V

V

V

P

P

L

M

F

F

H

H

C

V

N

N

G

A

W

W

C

C

H

L

T

P

W

Y

M

A

V

A

P

T

M

L

L

V

G

G

G

A

T

K

V

Q

V

V

C

L

C

P

R

G

D

P

V

R

T

L

A

D

K

P

A

A

I

S

Y

L

A

V

A

E

I

L

A

F

D

D

-

G

E

E

G

G

V

V

T

T

H

F

F

T

G

A

G

G

A

V

P

P

I

T

V

V

L

W

N

L

A

A

I

L

I

A

N

D

A

Y

G

L

A

E

E

S

D

T

R

G

R

H

D

R

F

L

G

K

H

T

V

L

V

R

E

R

V

L

F

V

T

V

A

G

G
|
G

A

S

P

A

P

P

A

R

A

S

T

L

L

I

A

A

A

R

I

F

E

E

P

R

L

M

G

G

F

V

N

E

V

V

T

R

Q
|
Q

V
x
G

Y

Y

G

G

L

L

T
|
T

E
|
E

V

T

Y

S

G

P

P

V

A

V

T

V

E

Q

C

N

I

F

W

V

K

K

P

S

E

H

W

L

D

E

A

S

T

L

T

S

G

E

D

E

E

E

R

K

A

L

A

T

L

L

K

K

A

A

R

K

T

T

G

G

V

L

G

P

M

I

A

P

T

L

L

V

E

R

G

-

A

L

E

R

V

V

H

A

D

D

T

E

H

E

G

G

Q

R

P

P

I

V

P

P

R

K

D

D

T

G

V

K

H

A

L

L

G

G

E

E

I

V

A

Q

M

L

R

K

G

G

N

P

M

W

V

I

M

T

K

G

G

G

Y

Y

K

G

N

N

P

E

E

E

A

A

T

T

A

R

E

S

A

A

F

L

K

T

-

P

D

D

G

G

W

F

F

F

R

R

S

T

G

G

D
|
D

I

I

A

A

F

V

Q

W

H

D

P

E

D

E

G

G

Y

Y

I

V

K

E

I

I

T

K

D

D

R

R

A

L

K
|
K

D

D

I

L

I

I

I
x
K

S

S

G

G

E

E

N

W

V

I

S

S

V

V

E

D

V

L

E

E

G

N

V

A

I

L

A

M

H

G

H

H

P

P

A

K

V

V

S

K

L

E

C

A

A

A

V

V

A

A

R

I

P

P

D

H

D

P

R

K

W

W

G

Q

E

E

V

R

P

P

C

L

A

A

F

V

V

V

E

-

L

V

K

P

R

R

G

G

R

E

Q

K

A

P

T

T

E

P

A

E

E

E

I

L

I

N

A

E

-

H

F

L

V

L

R

K

E

A

R

G

L

F

A

A

G

K

F

W

K

Q

T

L

P

P

K

D

Q

A

V

Y

I

V

F

F

C

A

-

E

D

E

L

I

P

P

K

R

T

T

S

S

T

A

G

G

K

K

I

F

Q

L

K

K

F

R

E

A

L

L

R

R

T184 (= T192) binding Mg(2+)
G302 (= G307) binding tetradecanoyl-AMP
Q322 (= Q327) binding tetradecanoyl-AMP
G323 (≠ V328) binding tetradecanoyl-AMP
T327 (= T332) binding tetradecanoyl-AMP
E328 (= E333) binding Mg(2+)
D418 (= D423) binding tetradecanoyl-AMP
K435 (= K440) binding tetradecanoyl-AMP
K439 (≠ I444) binding tetradecanoyl-AMP

P0DX84 3-methylmercaptopropionyl-CoA ligase; MMPA-CoA ligase; EC 6.2.1.44 from Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) (Silicibacter lacuscaerulensis) (see paper)
32% identity, 90% coverage: 44:543/553 of query aligns to 38:538/539 of P0DX84

query
sites
P0DX84

T

T

R

R

L

T

T

N

Y

W

G

G

E

E

Y

I

H

A

A

A

R

N

V

A

S

R

R

R

L

M

A

G

S

S

A

A

L

L

A

T

G

K

M

L

G

G

I

L

Q

Q

P

P

G

Q

D

D

V

R

V

I

A

G

T

T

I

L

L

A

P

W

N

N

I

N

A

R

Q

R

Q

H

A

L

E

E

A

I

H

Y

W

Y

G

A

V

A

P

S

A

G

A

A

G

G

A

F

I

V

L

C

N

H

T

T

I

I

N

N

I

P

R

R

L

L

D

F

V

P

E

E

T

Q

V

L

A

V

Y

Y

I

I

F

I

D

N

H

H

G

A

G

Q

A

D

K

R

L

V

V

L

L

F

C

F

D

D

T

A

A

T

F

F

L

L

P

P

L

L

A

V

R

A

A

A

A

I

C

R

E

D

K

Q

M

L

-

T

E

E

G

V

K

K

P

H

P

F

T

V

I

L

I

M

E

G

V

P

C

R

D

N

R

E

E

D

A

A

G

L

H

Q

S

Q

A

I

T

P

G

G

-

L

E

E

F

F

L

-

E

-

Y

Y

E

D

D

E

L

L

L

I

A

E

K

T

G

G

S

D

P

T

W

D

A

F

P

E

W

W

V

P

M

V

P

-

E

F

D

D

E

E

W

N

E

T

S

A

L

S

A

S

L

L

N

C

Y

Y

T

T

S

S

G

G

T

T

G

G

R

H

P

P

K

K

G

G

V

V

V

L

Y

Y

H

S

H

H

R

R

G

S

A

T

Y

V

L

L

A

H

T

S

Y

F

G

A

-

S

-

N

-

T

-

R

N

D

A

V

I

I

A

G

W

Y

R

S

M

A

Q

M

L

-

Y

-

P

D

V

V

Y

V

L

M

T

P

I

V

V

V

P

P

L

M

F

F

H
|
H

C

V

N

N

G

A

W
|
W

C

G

H

S

T

P

W

Y

M

G

V

C

P

A

M

M

L

S

G

G

G

A

T

Q

V

M

V

V

C

L

-

P

C

G

R

P

D

D

V

L

T

H

A

G

K

E

A

A

I

L

Y

V

A

N

A

L

I

I

A

D

D

T

E

Y

G

G

V

V

T

T

H

L

F

A

G

M

G

G

A

V

P

P

I

T

V

I

L

W

N

Q

A

G

I

L

N

A

A

H

G

A

A

A

E

K

D

C

R

G

R

T

D

K

F

L

G

E

H

S

V

L

V

E

E

R

V

T

F

V

T

I

A
x
G

G
|
G

A

A

P

A

P

C

P

P

A

P

A

S

T

M

L

I

A

A

A

T

I

F

-

R

E

E

P

K

L

Y

G

G

F

V

N

D

V

T

T

V

Q

H

V

A

Y
x
W

G

G

L

M

T

S

E

E

V

M

Y

S

G
x
P

P

L

A

G

T

T

E

A

C

N

I

I

W

P

K

L

P

A

E

K

W

H

D

R

A

K

T

L

T

P

G

I

D

E

E

E

R

Q

A

H

A

K

L

L

K

R

A

E

R

N

T

Q

G

G

V

R

G

P

M

P

A

F

T

G

L

V

E

E

G

-

A

L

E

K

V

I

H

V

D

D

T

D

H

D

G

G

Q

N

P

D

I

L

P

P

R

H

D

D

T

G

V

V

H

T

L

Q

G

G

E

D

I

L

A

M

M

V

R

R

G

G

N

H

M

W

V

V

M

L

K

D

G

S

Y

Y

Y

F

K

Q

N

L

P

K

E

D

A

Q

T

-

A

-

E

E

A

L

F

L

K

Q

D

D

G

G

W

W

F

F

R

A

S

T

G

G

D

D

I

V

A

A

F

T

Q

L

H

D

P

P

D

D

G

G

Y

Y

I

M

K

T

I

I

T

R

D

D

R
|
R

A

S

K
|
K

D
|
D

I

I

I
x
K

S

S

G
|
G

E
|
E

N
x
W

V

I

S

S

V

V

E

E

V

L

E

E

G

N

V

I

I

A

A

V

H

A

H

H

P

P

A

K

V

L

S

A

L

T

C

A

A

A

V

V

V

I

A

G

R

V

P

P

D

H

D

P

R

K

W

W

G

D

E
|
E

V

R

P

P

C

L

A

L

F

V

V

A

E

V

L

K

K

A

R

E

G

G

R

E

Q

D

A

P

T

S

E

E

A

A

E

E

I

L

A

E

F

F

V

F

R

D

E

G

R

K

L

I

A

A

G

K

F

W

K

Q

T

V

P

P

K

D

Q

K

V

V

I

V

F

F

C

V

D

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
|
K

I

V

Q

L

K
|
K

F

R

E

K

L

L

R

R

A

D

V

E

A

F

R

K

L

D

A

A

L

L

T

T

H231 (= H236) mutation to A: Retains 74% of wild-type activity.
W235 (= W240) mutation to A: Almost completely abolishes the activity.
G302 (≠ A306) mutation to P: Almost completely abolishes the activity.
G303 (= G307) mutation to P: Almost completely abolishes the activity.
W326 (≠ Y329) mutation to A: Retains 7.7% of wild-type activity.
P333 (≠ G336) mutation to A: Retains 69% of wild-type activity.
R432 (= R438) mutation to A: Retains 4.3% of wild-type activity.
K434 (= K440) mutation to A: Retains 36% of wild-type activity.
D435 (= D441) mutation to A: Retains 76% of wild-type activity.
K438 (≠ I444) mutation to A: Retains 5.6% of wild-type activity.
G440 (= G446) mutation to P: Retains 3.6% of wild-type activity.
G441 (= G447) mutation to P: Retains 2.7% of wild-type activity.
E442 (= E448) mutation to A: Retains 27% of wild-type activity.
W443 (≠ N449) mutation to A: Retains 60% of wild-type activity.
E474 (= E480) mutation to A: Retains 33% of wild-type activity.
K523 (= K528) Plays an important role in catalysis; mutation to A: Retains 1.6% of wild-type activity.; mutation to E: Retains 1.4% of wild-type activity.; mutation to R: Retains 57% of wild-type activity.
K526 (= K531) mutation to A: Retains 48% of wild-type activity.

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
32% identity, 90% coverage: 44:543/553 of query aligns to 38:538/538 of 6ijbB

query
sites
6ijbB

T

T

R

R

L

T

T

N

Y

W

G

G

E

E

Y

I

H

A

A

A

R

N

V

A

S

R

R

R

L

M

A

G

S

S

A

A

L

L

A

T

G

K

M

L

G

G

I

L

Q

Q

P

P

G

Q

D

D

V

R

V

I

A

G

T

T

I

L

L

A

P

W

N

N

I

N

A

R

Q

R

Q

H

A

L

E

E

A

I

H

Y

W

Y

G

A

V

A

P

S

A

G

A

A

G

G

A

F

I

V

L

C

N

H

T

T

I

I

N

N

I

P

R

R

L

L

D

F

V

P

E

E

T

Q

V

L

A

V

Y

Y

I

I

F

I

D

N

H

H

G

A

G

Q

A

D

K

R

L

V

V

L

L

F

C

F

D

D

T

A

A

T

F

F

L

L

P

P

L

L

A

V

R

A

A

A

A

I

C

R

E

D

K

Q

M

L

-

T

E

E

G

V

K

K

P

H

P

F

T

V

I

L

I

M

E

G

V

P

C

R

D

N

R

E

E

D

A

A

G

L

H

Q

S

Q

A

I

T

P

G

G

-

L

E

E

F

F

L

-

E

-

Y

Y

E

D

D

E

L

L

L

I

A

E

K

T

G

G

S

D

P

T

W

D

A

F

P

E

W

W

V

P

M

V

P

-

E

F

D

D

E

E

W

N

E

T

S

A

L

S

A

S

L

L

N

C

Y

Y

T
|
T

S

S

G

G

T

T

G

G

R

H

P

P

K

K

G

G

V

V

V

L

Y

Y

H

S

H

H

R

R

G

S

A

T

Y

V

L

L

A
x
H

T

S

Y

F

G

A

-

S

-

N

-

T

-

R

N

D

A

V

I

I

A

G

W

Y

R

S

M

A

Q

M

L

-

Y

-

P

D

V

V

Y

V

L

M

T

P

I

V

V

V

P

P

L

M

F

F

H
|
H

C

V

N

N

G

A

W
|
W

C

G

H

S

T

P

W

Y

M

G

V

C

P

A

M

M

L

S

G

G

G

A

T

Q

V

M

V

V

C

L

-

P

C

G

R

P

D

D

V

L

T

H

A

G

K

E

A

A

I

L

Y

V

A

N

A

L

I

I

A

D

D

T

E

Y

G

G

V

V

T

T

H

L

F

A

G

M

G

G

A

V

P

P

I

T

V

I

L

W

N

Q

A

G

I

L

N

A

A

H

G

A

A

A

E

K

D

C

R

G

R

T

D

K

F

L

G

E

H

S

V

L

V

E

E

R

V

T

F

V

T

I

A

G

G
|
G

A
|
A

P
x
A

P

C

P

P

A

P

A

S

T

M

L

I

A

A

A

T

I

F

-

R

E

E

P

K

L

Y

G

G

F

V

N

D

V

T

T

V

Q
x
H

V
x
A

Y
x
W

G
|
G

L
x
M

T
x
S

E
|
E

V

M

Y

S

G

P

P

L

A

G

T

T

E

A

C

N

I

I

W

P

K

L

P

A

E

K

W

H

D

R

A

K

T

L

T

P

G

I

D

E

E

E

R

Q

A

H

A

K

L

L

K

R

A

E

R

N

T
x
Q

G

G

V

R

G

P

M

P

A

F

T

G

L

V

E

E

G

-

A

L

E

K

V

I

H

V

D

D

T

D

H

D

G

G

Q

N

P

D

I

L

P

P

R

H

D

D

T

G

V

V

H

T

L

Q

G

G

E

D

I

L

A

M

M

V

R

R

G

G

N

H

M

W

V

V

M

L

K

D

G

S

Y

Y

Y

F

K

Q

N

L

P

K

E

D

A

Q

T

-

A

-

E

E

A

L

F

L

K

Q

D

D

G

G

W

W

F

F

R

A

S

T

G

G

D
|
D

I

V

A

A

F

T

Q

L

H

D

P

P

D

D

G

G

Y

Y

I

M

K

T

I

I

T

R

D

D

R

R

A

S

K

K

D

D

I

I

I
x
K

S

S

G

G

E

E

N
x
W

V

I

S

S

V

V

E

E

V

L

E

E

G

N

V

I

I

A

A

V

H

A

H

H

P

P

A

K

V

L

S

A

L

T

C

A

A

A

V

V

V

I

A

G

R

V

P

P

D

H

D

P

R

K

W

W

G

D

E

E

V

R

P

P

C

L

A

L

F

V

V

A

E

V

L

K

K

A

R

E

G

G

R

E

Q

D

A

P

T

S

E

E

A

A

E

E

I

L

A

E

F

F

V

F

R

D

E

G

R

K

L

I

A

A

G

K

F

W

K

Q

T

V

P

P

K

D

Q

K

V

V

I

V

F

F

C

V

D

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
x
A

I

V

Q

L

K

K

F

R

E

K

L

L

R

R

A

D

V

E

A

F

R

K

L

D

A

A

L

L

T

T

active site: T185 (= T192), H205 (≠ A212), H231 (= H236), S329 (≠ T332), E330 (= E333), K438 (≠ I444), W443 (≠ N449), A523 (≠ K528)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W240), G303 (= G307), A325 (≠ V328), W326 (≠ Y329), G327 (= G330), M328 (≠ L331)
binding adenosine monophosphate: G303 (= G307), A304 (= A308), A305 (≠ P309), H324 (≠ Q327), W326 (≠ Y329), G327 (= G330), M328 (≠ L331), S329 (≠ T332), Q359 (≠ T362), D417 (= D423)

8i3iA Acyl-acp synthetase structure bound to amp-pnp in the presence of mgcl2
33% identity, 89% coverage: 45:537/553 of query aligns to 35:520/522 of 8i3iA

query
sites
8i3iA

R

R

L

H

T

S

Y

Y

G

K

E

T

Y

F

H

H

A

D

R

R

V

V

S

R

R

Q

L

F

A

A

S

N

A

A

L

L

A

T

G

K

M

M

G

G

I

V

Q

K

P

K

G

G

D

D

V

T

V

V

A

A

T

V

I

M

L

D

P

Y

N

D

I

S

A

H

Q

R

Q

Y

A

L

E

E

A

C

H

Y

W

F

G

A

V

I

P

P

A

M

A

I

G

G

A

A

I

K

L

L

N

H

T

M

I

I

N

N

I

V

R

R

L

L

D

S

V

P

E

E

T

Q

V

I

A

L

Y

Y

I

T

F

I

D

D

H

H

G

A

G

E

A

D

K

D

L

I

V

I

L

L

C

I

D

H

T

E

A

E

F

F

L

L

P

P

L

I

A

-

R

-

A

-

C

L

E

D

K

Q

M

I

E

K

G

G

K

R

P

I

P

D

T

T

I

V

I

T

E

R

-

Y

V

V

C

V

D

L

R

R

E

D

A

-

G

-

H

-

S

-

A

-

T

-

G

D

E

E

F

E

L

C

E

E

Y

Y

E

E

D

R

L

L

A

E

K

Q

G

E

S

S

P

-

W

-

A

T

P

E

W

Y

V

N

M

F

P

P

E

D

-

F

D

D

E

E

W

N

E

T

S

V

L

A

A

T

L

T

N

F

Y

Y

T
|
T

S

T

G
|
G

T
|
T

G

G

R

F

P

P

K
|
K

G

G

V

V

V

F

Y

F

H

T

H

H

R

R

G

Q

A

L

Y

V

L

L

A

H

T

T

Y

M

G

G

-

I

-

L

-

S

-

T

-

I

-

G

-

T

N

N

A

A

I

S

A

Q

W

G

R

R

M

L

Q

H

L

Q

Y

G

P

D

V

I

Y

Y

L

M

T

P

I

I

V

T

P

P

L

M

F

F

H

H

C

V

N

H

G

A

W

W

C

G

H

L

T

P

W

Y

M

M

V

A

P

T

M

M

L

L

G

G

G

V

T

K

V

Q

V

V

C

Y

C

P

R

G

D

K

V

Y

T

V

A

P

K

D

A

V

I

L

Y

L

A

N

A

L

I

I

A

E

D

Q

E

E

G

K

V

V

T

T

H

F

F

S

G

H

G

C

A

V

P

P

I

T

V

I

L

L

N

H

A

L

I

L

N

S

A

S

G

P

A

K

E

S

D

K

R

A

R

M

D

D

F

F

G

S

H

G

V

W

V

K

E

V

V

V

F

I

T

G

A
x
G

G
x
A

A
|
A

P

L

P

P

P

K

A

A

A

L

T

C

L

K

A

S

A

A

I

L

E

E

P

R

L

-

G

D

F

I

N

D

V

V

T

F

Q

A

V

G

Y
|
Y

G

G

L
x
M

T
x
S

E

E

V

T

Y

-

G

G

P

P

A

I

T

L

E

S

C

I

I

V

-

Q

W

L

K

T

P

P

E

E

W

Q

D

L

A

E

T

L

T

D

G

V

D

D

E

Q

R

Q

A

A

A

E

L

Y

K

R

A

S

R

K

T

T

G

G

V

K

G

K

M

V

A

A

T

L

L

V

E

E

G

-

A

A

E

Y

V

I

H

V

D

D

T

E

H

D

G

M

Q

N

P

K

I

L

P

P

R

H

D

D

T

G

V

E

H

T

L

A

G

G

E

E

I

I

A

V

M

V

R

R

G

A

N

P

M

W

V

L

M

T

K

P

G

N

Y

Y

K

K

N

D

P

N

E

K

A

N

T

S

A

K

E

A

A

L

F

W

K

R

D

G

G

G

W

Y

F

L

R

H

S

T

G

G

D
|
D

I

V

A

A

F

H

Q

I

H

D

P

D

D

E

G

G

Y

F

I

I

K

K

I

I

T

T

D

D

R
|
R

A

V

K

K

D

D

I

M

I

I

I

K

S

I

G

S

G

G

E

E

N

W

V

V

S

S

V

L

E

E

V

L

E

E

G

D

V

I

I

L

A

H

H

Q

H

H

P

Q

A

S

V

V

S

S

L

E

C

V

A

A

V

V

V

I

A

G

R

M

P

P

D

H

D

N

R

K

W

W

G

G

E

E

V

V

P

P

C

L

A

A

F

L

V

V

E

T

L

L

K

K

R

E

G

D

R

A

Q

Q

A

V

T

T

E

E

A

K

E

E

I

L

A

G

F

F

V

A

R

K

E

D

R

R

L

E

A

A

G

L

F

L

K

L

T

K

P

V

K

K

Q

-

V

-

I

I

F

V

C

D

D

E

L

I

P

A

K

K

T

T

S

S

T

V

G

G

K

K

I

V

Q

D

K

K

F

K

E

E

L

L

R

R

A

K

V

L

binding phosphoaminophosphonic acid-adenylate ester: T172 (= T192), G174 (= G194), T175 (= T195), T176 (= T196), K180 (= K200), G293 (≠ A306), A294 (≠ G307), A295 (= A308), Y315 (= Y329), M317 (≠ L331), S318 (≠ T332), D408 (= D423), R423 (= R438)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
34% identity, 88% coverage: 26:510/553 of query aligns to 17:487/491 of 1v25A

query
sites
1v25A

L

L

K

E

R

R

A

A

E

A

V

L

F

F

T

G

D

R

R

K

-

E

-

V

I

V

A

S

L

R

V

L

H

H

G

T

A

G

T

E

-

V

-

H

R

R

L

T

T

T

Y

Y

G

A

E

E

Y

V

H

Y

A

Q

R

R

V

A

S

R

R

R

L

L

A

M

S

G

A

G

L

L

A

R

G

A

M

L

G

G

I

V

Q

G

P

V

G

G

D

D

V

R

V

V

A

A

T

T

I

L

L

G

P

F

N

N

I

H

A

F

Q

R

Q

H

A

L

E

E

A

A

H

Y

W

F

G

A

V

V

P

P

A

G

A

M

G

G

A

A

I

V

L

L

N

H

T

T

I

A

N

N

I

P

R

R

L

L

D

S

V

P

E

K

T

E

V

I

A

A

Y

Y

I

I

F

L

D

N

H

H

G

A

G

E

A

D

K

K

L

V

V

L

L

L

C

F

D

D

T

P

A

N

F

L

L

L

P

P

L

L

A

-

R

-

A

-

C

V

E

E

K

A

M

I

E

R

G

G

K

E

P

L

P

K

T

T

I

V

-

Q

-

H

I

F

E

V

V

V

C

M

D

D

R

E

E

K

A

A

G

P

H

E

S

G

A

-

T

-

G

-

E

-

F

Y

L

L

E

A

Y

Y

E

E

D

E

L

A

L

L

A

-

K

-

G

G

S

E

P

E

W

A

A

D

P

P

W

V

V

R

M

V

P

P

E

E

D

R

E

-

W

-

E

A

S

A

L

C

A

G

L

M

N

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

R

L

P

P

K

K

G

G

V

V

Y

Y

H

S

H

H

R

R

G

A

A

L

Y

V

L

L

A
x
H

T

S

Y

L

G

A

N

A

A

S

I

L

A

V

W

D

R

G

M

T

Q

A

L

L

Y

S

P

E

-

K

-

D

V

V

Y

V

L

L

T

P

I

V

V

V

P

P

L

M

F

F

H
|
H

C
x
V

N

N

G

A

W

W

C

C

H

L

T

P

W

Y

M

A

V

A

P

T

M

L

L

V

G

G

G

A

T

K

V

Q

V

V

C

L

C

P

R

G

D

P

V

R

T

L

A

D

K

P

A

A

I

S

Y

L

A

V

A

E

I

L

A

F

D

D

-

G

E

E

G

G

V

V

T

T

H

F

F

T

G

A

G

G

A

V

P

P

I

T

V

V

L

W

N

L

A

A

I

L

I

A

N

D

A

Y

G

L

A

E

E

S

D

T

R

G

R

H

D

R

F

L

G

K

H

T

V

L

V

R

E

R

V

L

F

V

T

V

A

G

G
|
G

A
x
S

P
x
A

P

A

P

P

A

R

A

S

T

L

L

I

A

A

A

R

I

F

E

E

P

R

L

M

G

G

F

V

N

E

V

V

T

R

Q

Q

V

G

Y
|
Y

G
|
G

L
|
L

T
|
T

E
|
E

V

T

Y

S

G

P

P

V

A

V

T

V

E

Q

C

N

I

F

W

V

K

K

P

S

E

H

W

L

D

E

A

S

T

L

T

S

G

E

D

E

E

E

R

K

A

L

A

T

L

L

K

K

A

A

R

K

T

T

G

G

V

L

G

P

M

I

A

P

T

L

L

V

E

R

G

-

A

L

E

R

V

V

H

A

D

D

T

E

H

E

G

G

Q

R

P

P

I

V

P

P

R

K

D

D

T

G

V

K

H

A

L

L

G

G

E

E

I

V

A

Q

M

L

R

K

G

G

N

P

M

W

V

I

M

T

K

G

G

G

Y

Y

K

G

N

N

P

E

E

E

A

A

T

T

A

R

E

S

A

A

F

L

K

T

-

P

D

D

G

G

W

F

F

F

R

R

S

T

G

G

D
|
D

I

I

A

A

F

V

Q

W

H

D

P

E

D

E

G

G

Y

Y

I

V

K

E

I
|
I

T

K

D

D

R

R

A

L

K

K

D

D

I

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

V

I

S

S

V

V

E

D

V

L

E

E

G

N

V

-

I

-

A

-

H

-

P

-

A

-

V

-

S

-

L

-

C

A

A

A

V

V

A

A

R

I

P

P

D

H

D

P

R

K

W

W

G

Q

E

E

V

R

P

P

C

L

A

A

F

V

V

V

E

G

L

F

K

A

R

K

G

W

R

-

Q

Q

A

L

T

P

E

D

A

A

E

Y

I

L

I

K

A

R

F

A

V

L

R

R

E

E

R

Q

L

Y

A

K

G

N

F

Y

active site: T177 (= T192), H197 (≠ A212), H223 (= H236), T320 (= T332), E321 (= E333), K432 (≠ I444), W437 (≠ N449)
binding phosphoaminophosphonic acid-adenylate ester: H223 (= H236), V224 (≠ C237), G295 (= G307), S296 (≠ A308), A297 (≠ P309), Y317 (= Y329), G318 (= G330), L319 (= L331), T320 (= T332), D411 (= D423), I423 (= I435), K432 (≠ I444), W437 (≠ N449)
binding magnesium ion: T177 (= T192), E321 (= E333)

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
35% identity, 83% coverage: 26:484/553 of query aligns to 17:463/510 of 1v26B

query
sites
1v26B

L

L

K

E

R

R

A

A

E

A

V

L

F

F

T

G

D

R

R

K

-

E

-

V

I

V

A

S

L

R

V

L

H

H

G

T

A

G

T

E

-

V

-

H

R

R

L

T

T

T

Y

Y

G

A

E

E

Y

V

H

Y

A

Q

R

R

V

A

S

R

R

R

L

L

A

M

S

G

A

G

L

L

A

R

G

A

M

L

G

G

I

V

Q

G

P

V

G

G

D

D

V

R

V

V

A

A

T

T

I

L

L

G

P

F

N

N

I

H

A

F

Q

R

Q

H

A

L

E

E

A

A

H

Y

W

F

G

A

V

V

P

P

A

G

A

M

G

G

A

A

I

V

L

L

N

H

T

T

I

A

N

N

I

P

R

R

L

L

D

S

V

P

E

K

T

E

V

I

A

A

Y

Y

I

I

F

L

D

N

H

H

G

A

G

E

A

D

K

K

L

V

V

L

L

L

C

F

D

D

T

P

A

N

F

L

L

L

P

P

L

L

A

-

R

-

A

-

C

V

E

E

K

A

M

I

E

R

G

G

K

E

P

L

P

K

T

T

I

V

-

Q

-

H

I

F

E

V

V

V

C

M

D

D

R

E

E

K

A

A

G

P

H

E

S

G

A

-

T

-

G

-

E

-

F

Y

L

L

E

A

Y

Y

E

E

D

E

L

A

L

L

A

-

K

-

G

G

S

E

P

E

W

A

A

D

P

P

W

V

V

R

M

V

P

P

E

E

D

R

E

-

W

-

E

A

S

A

L

C

A

G

L

M

N

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

R

L

P

P

K

K

G

G

V

V

Y

Y

H

S

H

H

R

R

G

A

A

L

Y

V

L

L

A
x
H

T

S

Y

L

G

A

N

A

A

S

I

L

A

V

W

D

R

G

M

T

Q

A

L

L

Y

S

P

E

-

K

-

D

V

V

Y

V

L

L

T

P

I

V

V

V

P

P

L

M

F

F

H
|
H

C

V

N

N

G

A

W

W

C

C

H

L

T

P

W

Y

M

A

V

A

P

T

M

L

L

V

G

G

G

A

T

K

V

Q

V

V

C

L

C

P

R

G

D

P

V

R

T

L

A

D

K

P

A

A

I

S

Y

L

A

V

A

E

I

L

A

F

D

D

-

G

E

E

G

G

V

V

T

T

H

F

F

T

G

A

G

G

A

V

P

P

I

T

V

V

L

W

N

L

A

A

I

L

I

A

N

D

A

Y

G

L

A

E

E

S

D

T

R

G

R

H

D

R

F

L

G

K

H

T

V

L

V

R

E

R

V

L

F

V

T

V

A

G

G
|
G

A
x
S

P
x
A

P

A

P

P

A

R

A

S

T

L

L

I

A

A

A

R

I

F

E

E

P

R

L

M

G

G

F

V

N

E

V

V

T

R

Q

Q

V
x
G

Y
|
Y

G
|
G

L
|
L

T
|
T

E
|
E

V

T

Y

S

G

P

P

V

A

V

T

V

E

Q

C

N

I

F

W

V

K

K

P

S

E

H

W

L

D

E

A

S

T

L

T

S

G

E

D

E

E

E

R

K

A

L

A

T

L

L

K

K

A

A

R

K

T

T

G

G

V

L

G

P

M

I

A

P

T

L

L

V

E

R

G

-

A

L

E

R

V

V

H

A

D

D

T

E

H

E

G

G

Q

R

P

P

I

V

P

P

R

K

D

D

T

G

V

K

H

A

L

L

G

G

E

E

I

V

A

Q

M

L

R

K

G

G

N

P

M

W

V

I

M

T

K

G

G

G

Y

Y

K

G

N

N

P

E

E

E

A

A

T

T

A

R

E

S

A

A

F

L

K

T

-

P

D

D

G

G

W

F

F

F

R

R

S

T

G

G

D
|
D

I

I

A

A

F

V

Q

W

H

D

P

E

D

E

G

G

Y

Y

I

V

K

E

I

I

T

K

D

D

R

R

A

L

K
|
K

D

D

I

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

V

I

S

S

V

V

E

D

V

L

E

E

G

N

V

A

I

-

A

-

H

-

P

-

A

-

V

-

S

-

L

-

C

A

A

A

V

V

A

A

R

I

P

P

D

H

D

P

R

K

W

W

G

Q

E

E

V

R

P

P

C

L

A

A

active site: T177 (= T192), H197 (≠ A212), H223 (= H236), T320 (= T332), E321 (= E333), K432 (≠ I444), W437 (≠ N449)
binding adenosine monophosphate: G295 (= G307), S296 (≠ A308), A297 (≠ P309), G316 (≠ V328), Y317 (= Y329), G318 (= G330), L319 (= L331), T320 (= T332), D411 (= D423), K428 (= K440), K432 (≠ I444), W437 (≠ N449)
binding magnesium ion: T177 (= T192), E321 (= E333)

8i6mA Acyl-acp synthetase structure bound to amp-c18:1
32% identity, 89% coverage: 45:537/553 of query aligns to 33:526/528 of 8i6mA

query
sites
8i6mA

R

R

L

H

T

S

Y

Y

G

K

E

T

Y

F

H

H

A

D

R

R

V

V

S

R

R

Q

L

F

A

A

S

N

A

A

L

L

A

T

G

K

M

M

G

G

I

V

Q

K

P

K

G

G

D

D

V

T

V

V

A

A

T

V

I

M

L

D

P

Y

N

D

I

S

A

H

Q

R

Q

Y

A

L

E

E

A

C

H

Y

W

F

G

A

V

I

P

P

A

M

A

I

G

G

A

A

I

K

L

L

N

H

T

M

I

I

N

N

I

V

R

R

L

L

D

S

V

P

E

E

T

Q

V

I

A

L

Y

Y

I

T

F

I

D

D

H

H

G

A

G

E

A

D

K

D

L

I

V

I

L

L

C

I

D

H

T

E

A

E

F

F

L

L

P

P

L

I

A

-

R

-

A

-

C

L

E

D

K

Q

M

I

E

K

G

G

K

R

P

I

P

D

T

T

I

V

I

T

E

R

-

Y

V

V

C

V

D

L

R

R

E

D

A

-

G

-

H

-

S

-

A

-

T

-

G

D

E

E

F

E

L

C

E

E

Y

Y

E

E

D

R

L

L

A

E

K

Q

G

E

S

S

P

-

W

-

A

T

P

E

W

Y

V

N

M

F

P

P

E

D

-

F

D

D

E

E

W

N

E

T

S

V

L

A

A

T

L

T

N

F

Y

Y

T

T

S

T

G

G

T

T

G

G

R

F

P

P

K

K

G

G

V

V

V

F

Y

F

H

T

H

H

R

R

G

Q

A

L

Y

V

L

L

A

H

T

T

Y

M

G

G

-

I

-

L

-

S

-

T

-

I

-

G

-

T

N

N

A

A

I

S

A

Q

W

G

R

R

M

L

Q

H

L

Q

Y

G

P

D

V

I

Y

Y

L

M

T

P

I

I

V

T

P

P

L

M

F

F

H

H

C

V

N

H

G

A

W

W

C

G

H

L

T

P

W

Y

M

M

V

A

P

T

M

M

L

L

G

G

G

V

T

K

V

Q

V

V

C

Y

C

P

R

G

D

K

V

Y

T

V

A

P

K

D

A

V

I

L

Y

L

A

N

A

L

I

I

A

E

D

Q

E

E

G

K

V

V

T

T

H

F

F

S

G

H

G

C

A

V

P

P

I

T

V

I

L

L

N

H

A

L

I

L

N

S

A

S

G

P

A

K

E

S

D

K

R

A

R

M

D

D

F

F

G

S

H

G

V

W

V

K

E

V

V

V

F

I

T

G

A
x
G

G

A

A
|
A

P

L

P

P

P

K

A

A

A

L

T

C

L

K

A

S

A

A

I

L

E

E

P

R

L

-

G

D

F

I

N

D

V

V

T

F

Q

A

V
x
G

Y
|
Y

G
|
G

L
x
M

T
x
S

E
|
E

V

T

Y

-

G

G

P

P

A

I

T

L

E

S

C

I

I

V

-

Q

W

L

K

T

P

P

E

E

W

Q

D

L

A

E

T

L

T

D

G

V

D

D

E

Q

R

Q

A

A

A

E

L

Y

K

R

A

S

R

K

T

T

G

G

V

K

G

K

M

V

A

A

T

L

L

V

E

E

G

-

A

A

E

Y

V

I

H

V

D

D

T

E

H

D

G

M

Q

N

P

K

I

L

P

P

R

H

D

D

T

G

V

E

H

T

L

A

G

G

E

E

I

I

A

V

M

V

R

R

G

A

N

P

M

W

V

L

M

T

K

P

G

N

Y

Y

K

K

N

D

P

N

E

K

A

N

T

S

A

K

E

A

A

L

F

W

K

R

D

G

G

G

W

Y

F

L

R

H

S

T

G

G

D
|
D

I

V

A

A

F

H

Q

I

H

D

P

D

D

E

G

G

Y

F

I

I

K

K

I

I

T

T

D

D

R
|
R

A

V

K

K

D

D

I

M

I

I

I

K

S

I

G

S

G

G

E

E

N

W

V

V

S

S

V

L

E

E

V

L

E

E

G

D

V

I

I

L

A

H

H

Q

H

H

P

Q

A

S

V

V

S

S

L

E

C

V

A

A

V

V

V

I

A

G

R

M

P

P

D

H

D

N

R

K

W

W

G

G

E

E

V

V

P

P

C

L

A

A

F

L

V

V

E

T

L

L

K

K

R

E

G

D

R

A

Q

Q

A

V

T

T

E

E

A

K

E

E

I

L

A

G

F

F

V

A

R

K

E

D

R

F

L

I

A

N

G

K

F

G

K

I

T

L

P

A

K

R

Q

E

V

A

I

L

F

L

C

L

-

K

-

V

-

K

-

I

-

V

-

D

D

E

L

I

P

A

K

K

T

T

S

S

T

V

G

G

K

K

I

V

Q

D

K

K

F

K

E

E

L

L

R

R

A

K

V

L

binding adenosine monophosphate: G291 (≠ A306), A293 (= A308), G312 (≠ V328), Y313 (= Y329), G314 (= G330), M315 (≠ L331), S316 (≠ T332), D406 (= D423), R421 (= R438)
binding magnesium ion: M315 (≠ L331), S316 (≠ T332), E317 (= E333)

8i51A Acyl-acp synthetase structure bound to amp-mc7
32% identity, 89% coverage: 45:537/553 of query aligns to 33:526/528 of 8i51A

query
sites
8i51A

R

R

L

H

T

S

Y

Y

G

K

E

T

Y

F

H

H

A

D

R

R

V

V

S

R

R

Q

L

F

A

A

S

N

A

A

L

L

A

T

G

K

M

M

G

G

I

V

Q

K

P

K

G

G

D

D

V

T

V

V

A

A

T

V

I

M

L

D

P

Y

N

D

I

S

A

H

Q

R

Q

Y

A

L

E

E

A

C

H

Y

W

F

G

A

V

I

P

P

A

M

A

I

G

G

A

A

I

K

L

L

N

H

T

M

I

I

N

N

I

V

R

R

L

L

D

S

V

P

E

E

T

Q

V

I

A

L

Y

Y

I

T

F

I

D

D

H

H

G

A

G

E

A

D

K

D

L

I

V

I

L

L

C

I

D

H

T

E

A

E

F

F

L

L

P

P

L

I

A

-

R

-

A

-

C

L

E

D

K

Q

M

I

E

K

G

G

K

R

P

I

P

D

T

T

I

V

I

T

E

R

-

Y

V

V

C

V

D

L

R

R

E

D

A

-

G

-

H

-

S

-

A

-

T

-

G

D

E

E

F

E

L

C

E

E

Y

Y

E

E

D

R

L

L

A

E

K

Q

G

E

S

S

P

-

W

-

A

T

P

E

W

Y

V

N

M

F

P

P

E

D

-

F

D

D

E

E

W

N

E

T

S

V

L

A

A

T

L

T

N

F

Y

Y

T

T

S

T

G

G

T

T

G

G

R

F

P

P

K

K

G

G

V

V

V

F

Y

F

H

T

H

H

R

R

G

Q

A

L

Y

V

L

L

A

H

T

T

Y

M

G

G

-

I

-

L

-

S

-

T

-

I

-

G

-

T

N

N

A

A

I

S

A

Q

W

G

R

R

M

L

Q

H

L

Q

Y

G

P

D

V

I

Y

Y

L

M

T

P

I

I

V

T

P

P

L

M

F

F

H

H

C

V

N

H

G

A

W
|
W

C

G

H

L

T

P

W

Y

M

M

V

A

P

T

M

M

L

L

G

G

G

V

T

K

V

Q

V

V

C

Y

C

P

R

G

D

K

V

Y

T

V

A

P

K

D

A

V

I

L

Y

L

A

N

A

L

I

I

A

E

D

Q

E

E

G

K

V

V

T

T

H

F

F

S

G

H

G

C

A

V

P

P

I

T

V

I

L

L

N

H

A

L

I

L

N

S

A

S

G

P

A

K

E

S

D

K

R

A

R

M

D

D

F

F

G

S

H

G

V

W

V

K

E

V

V

V

F

I

T
x
G

A
x
G

G

A

A
|
A

P

L

P

P

P

K

A

A

A

L

T

C

L

K

A

S

A

A

I

L

E

E

P

R

L

-

G

D

F

I

N

D

V

V

T

F

Q

A

V
x
G

Y
|
Y

G
|
G

L
x
M

T
x
S

E

E

V

T

Y

-

G

G

P
|
P

A
x
I

T

L

E

S

C

I

I

V

-

Q

W

L

K

T

P

P

E

E

W

Q

D

L

A

E

T

L

T

D

G

V

D

D

E

Q

R

Q

A

A

A

E

L

Y

K

R

A

S

R

K

T

T

G

G

V

K

G

K

M

V

A

A

T

L

L

V

E

E

G

-

A

A

E

Y

V

I

H

V

D

D

T

E

H

D

G

M

Q

N

P

K

I

L

P

P

R

H

D

D

T

G

V

E

H

T

L

A

G

G

E

E

I

I

A

V

M

V

R

R

G

A

N

P

M

W

V

L

M

T

K

P

G

N

Y

Y

K

K

N

D

P

N

E

K

A

N

T

S

A

K

E

A

A

L

F

W

K

R

D

G

G

G

W

Y

F

L

R

H

S

T

G

G

D
|
D

I

V

A

A

F

H

Q

I

H

D

P

D

D

E

G

G

Y

F

I

I

K

K

I

I

T

T

D

D

R
|
R

A

V

K

K

D

D

I

M

I

I

I

K

S

I

G

S

G

G

E

E

N

W

V

V

S

S

V

L

E

E

V

L

E

E

G

D

V

I

I

L

A

H

H

Q

H

H

P

Q

A

S

V

V

S

S

L

E

C

V

A

A

V

V

V

I

A

G

R

M

P

P

D

H

D

N

R

K

W

W

G

G

E

E

V

V

P

P

C

L

A

A

F

L

V

V

E

T

L

L

K

K

R

E

G

D

R

A

Q

Q

A

V

T

T

E

E

A

K

E

E

I

L

A

G

F

F

V

A

R

K

E

D

R

F

L

I

A

N

G

K

F

G

K

I

T

L

P

A

K

R

Q

E

V

A

I

L

F

L

C

L

-

K

-

V

-

K

-

I

-

V

-

D

D

E

L

I

P

A

K

K

T

T

S

S

T

V

G

G

K

K

I

V

Q

D

K

K

F

K

E

E

L

L

R

R

A

K

V

L

binding adenosine monophosphate: G291 (≠ A306), A293 (= A308), Y313 (= Y329), M315 (≠ L331), S316 (≠ T332), D406 (= D423), R421 (= R438)
binding 7-methoxy-7-oxidanylidene-heptanoic acid: W225 (= W240), G290 (≠ T305), G312 (≠ V328), G314 (= G330), M315 (≠ L331), P320 (= P337), I321 (≠ A338)

8i8dA Acyl-acp synthetase structure bound to mc7-acp
32% identity, 89% coverage: 45:537/553 of query aligns to 35:528/529 of 8i8dA

query
sites
8i8dA

R

R

L

H

T

S

Y

Y

G

K

E

T

Y

F

H

H

A

D

R

R

V

V

S

R

R

Q

L

F

A

A

S

N

A

A

L

L

A

T

G

K

M

M

G

G

I

V

Q

K

P

K

G

G

D

D

V

T

V

V

A

A

T

V

I

M

L

D

P

Y

N

D

I

S

A

H

Q

R

Q

Y

A

L

E

E

A

C

H

Y

W

F

G

A

V

I

P

P

A

M

A

I

G

G

A

A

I

K

L

L

N

H

T

M

I

I

N

N

I

V

R
|
R

L

L

D

S

V

P

E

E

T

Q

V

I

A

L

Y

Y

I

T

F

I

D

D

H

H

G

A

G

E

A

D

K

D

L

I

V

I

L

L

C

I

D

H

T

E

A

E

F

F

L

L

P

P

L

I

A

-

R

-

A

-

C

L

E

D

K

Q

M

I

E

K

G

G

K

R

P

I

P

D

T

T

I

V

I

T

E

R

-

Y

V

V

C

V

D

L

R

R

E

D

A

-

G

-

H

-

S

-

A

-

T

-

G

D

E

E

F

E

L

C

E

E

Y

Y

E

E

D

R

L

L

A

E

K

Q

G

E

S

S

P

-

W

-

A

T

P

E

W

Y

V

N

M

F

P

P

E

D

-

F

D

D

E

E

W

N

E

T

S

V

L

A

A

T

L

T

N

F

Y

Y

T

T

S

T

G

G

T

T

G

G

R

F

P

P

K

K

G

G

V

V

V

F

Y

F

H

T

H

H

R

R

G

Q

A

L

Y

V

L

L

A

H

T

T

Y

M

G

G

-

I

-

L

-

S

-

T

-

I

-

G

-

T

N

N

A

A

I

S

A

Q

W

G

R

R

M

L

Q

H

L

Q

Y

G

P

D

V

I

Y

Y

L

M

T

P

I

I

V

T

P
|
P

L

M

F

F

H
|
H

C

V

N

H

G

A

W
|
W

C

G

H

L

T

P

W

Y

M

M

V

A

P

T

M

M

L

L

G

G

G

V

T

K

V

Q

V

V

C

Y

C

P

R

G

D

K

V

Y

T

V

A

P

K

D

A

V

I

L

Y

L

A

N

A

L

I

I

A

E

D

Q

E

E

G

K

V

V

T

T

H

F

F

S

G

H

G

C

A

V

P

P

I

T

V
x
I

L

L

N

H

A

L

I

L

N

S

A

S

G

P

A

K

E

S

D

K

R

A

R

M

D

D

F

F

G

S

H

G

V

W

V

K

E

V

V

V

F

I

T
x
G

A
x
G

G

A

A
|
A

P

L

P

P

P

K

A

A

A

L

T

C

L

K

A

S

A

A

I

L

E

E

P

R

L

-

G

D

F

I

N

D

V

V

T

F

Q

A

V
x
G

Y
|
Y

G
|
G

L
x
M

T
x
S

E

E

V

T

Y

-

G

G

P
|
P

A

I

T

L

E

S

C

I

I

V

-

Q

W

L

K

T

P

P

E

E

W

Q

D

L

A

E

T

L

T

D

G

V

D

D

E

Q

R

Q

A

A

A

E

L

Y

K

R

A

S

R

K

T

T

G

G

V

K

G

K

M

V

A

A

T

L

L

V

E

E

G

-

A

A

E

Y

V

I

H

V

D

D

T

E

H

D

G

M

Q

N

P

K

I

L

P

P

R

H

D

D

T

G

V

E

H

T

L

A

G

G

E

E

I

I

A

V

M

V

R

R

G

A

N

P

M

W

V

L

M

T

K

P

G

N

Y

Y

K

K

N

D

P

N

E

K

A

N

T

S

A

K

E

A

A

L

F

W

K

R

D

G

G

G

W

Y

F

L

R

H

S

T

G

G

D
|
D

I

V

A

A

F

H

Q

I

H

D

P

D

D

E

G

G

Y

F

I

I

K

K

I

I

T

T

D

D

R

R

A

V

K

K

D

D

I

M

I

I

I
x
K

S

I

G

S

G
|
G

E

E

N

W

V

V

S

S

V

L

E

E

V

L

E

E

G

D

V

I

I

L

A

H

H

Q

H

H

P

Q

A

S

V

V

S

S

L

E

C

V

A

A

V

V

V

I

A

G

R

M

P

P

D

H

D

N

R

K

W

W

G

G

E

E

V

V

P

P

C

L

A

A

F

L

V

V

E

T

L

L

K

K

R

E

G

D

R

A

Q

Q

A

V

T

T

E

E

A

K

E

E

I

L

A

G

F

F

V

A

R

K

E

D

R

F

L

I

A

N

G

K

F

G

K

I

T

L

P

A

K

R

Q

E

V

A

I

L

F

L

C

L

-

K

-

V

-

K

-

I

-

V

-

D

D

E

L

I

P

A

K

K

T

T

S

S

T

V

G

G

K

K

I

V

Q

D

K

K

F

K

E

E

L

L

R

R

A

K

V

L

binding adenosine monophosphate: G292 (≠ T305), G293 (≠ A306), A295 (= A308), G314 (≠ V328), Y315 (= Y329), G316 (= G330)