SitesBLAST

P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 46% coverage: 3:166/360 of query aligns to 74:237/496 of P39976

query
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P39976

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Sites not aligning to the query:

17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
29% identity, 48% coverage: 1:173/360 of query aligns to 104:276/521 of Q8N465

query
sites
Q8N465

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S109 (≠ E6) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ G24) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (≠ R28) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
I147 (vs. gap) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ L43) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ T62) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (= P79) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A102) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (≠ G128) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
G233 (= G130) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; uncertain significance)
375 D → Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
386 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; R→A: Loss of catalytic activity.
390 binding (R)-2-hydroxyglutarate; binding (R)-malate; T→A: Significantly reduced catalytic activity.
399 V → M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
401 binding (R)-2-hydroxyglutarate; binding (R)-malate; K→A: Loss of catalytic activity.
419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
434 binding Zn(2+); H→A: Loss of catalytic activity.
436 G → V: slight reduction in catalytic activity
439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
441 binding Zn(2+); H→A: Loss of catalytic activity.
443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
475 binding Zn(2+); E→A: Loss of catalytic activity.
476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
29% identity, 48% coverage: 1:173/360 of query aligns to 51:223/466 of 6lpxA

query
sites
6lpxA

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binding flavin-adenine dinucleotide: P75 (≠ G25), Q76 (≠ G26), G77 (≠ T27), G78 (≠ R28), N79 (≠ G29), T80 (≠ I30), G81 (= G31), M82 (≠ R32), G85 (= G35), S86 (≠ A36), L139 (= L89), G140 (= G90), A141 (≠ R91), C145 (≠ S95), G149 (= G99), N150 (≠ V100), A152 (= A102), T153 (≠ S103), G157 (= G107), G207 (= G157), I212 (≠ L162)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 333, 337, 348, 379, 381, 388, 423
binding flavin-adenine dinucleotide: 39, 422, 459
binding zinc ion: 381, 388, 422

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
29% identity, 48% coverage: 1:173/360 of query aligns to 51:223/466 of 6lpuA

query
sites
6lpuA

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binding flavin-adenine dinucleotide: P75 (≠ G25), G77 (≠ T27), G78 (≠ R28), N79 (≠ G29), T80 (≠ I30), G81 (= G31), G85 (= G35), S86 (≠ A36), L139 (= L89), G140 (= G90), A141 (≠ R91), C145 (≠ S95), H146 (≠ T96), G148 (= G98), G149 (= G99), N150 (≠ V100), A152 (= A102), T153 (≠ S103), A155 (= A105), E206 (≠ H156), G207 (= G157), I211 (≠ V161), I212 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39, 422, 459
binding (2s)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 423
binding zinc ion: 381, 388, 422

6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
29% identity, 48% coverage: 1:173/360 of query aligns to 51:223/466 of 6lpqA

query
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6lpqA

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binding flavin-adenine dinucleotide: P75 (≠ G25), G77 (≠ T27), G78 (≠ R28), N79 (≠ G29), T80 (≠ I30), G81 (= G31), G85 (= G35), S86 (≠ A36), L139 (= L89), G140 (= G90), A141 (≠ R91), C145 (≠ S95), H146 (≠ T96), G149 (= G99), N150 (≠ V100), A152 (= A102), T153 (≠ S103), A155 (= A105), G157 (= G107), E206 (≠ H156), G207 (= G157), I211 (≠ V161), I212 (≠ L162)
binding d-malate: M82 (≠ R32)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39, 422, 459
binding d-malate: 333, 337, 348, 379, 381, 388, 422, 423
binding zinc ion: 381, 388, 422

6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
29% identity, 48% coverage: 1:173/360 of query aligns to 51:223/466 of 6lppA

query
sites
6lppA

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x
P

G

Q

T
x
G

R
x
G

G
x
N

I
x
T

G
|
G

R
x
M

C

V

A

G

G
|
G

A
x
S

V

V

-

P

-

V

-

F

-

D

-

E

-

I

L

L

E

S

T

T

C

A

G

R

L

M

A

N

G

R

I

V

R

L

L

S

Y

F

E

H

P

S

G

V

A

S

L

G

T

I

L

L

V

V

A

C

G

Q

A

A

G

G

T

C

R

V

L

L

A

E

D

E

V

L

E

S

A

R

A

Y

L

V

A

E

Q

R

G

D

Q

F

R

I

L

M

P

P

F

L

E

D

V

-

P

-

D

-

L

-

R

-

G

-

L

-

L
|
L

G
|
G

R
x
A

G

K

G

G

C

S

S
x
C

T

H

L

I

G

G

G
|
G

V
x
N

V

V

A
|
A

S
x
T

N

N

A
|
A

S

G

G
|
G

P

L

R

R

R

F

V

L

Q

R

A

Y

G

G

A

S

C

L

R

H

D

G

S

T

L

V

I

L

G

G

V

L

R

E

F

V

V

V

D

L

G

A

T

D

G

G

A

T

V

V

V

L

K

D

N

C

G

L

G

T

R

S

V

L

M

R

K

K

N

D

V

N

T

T

G

G

Y

Y

D

D

L

L

V

K

K

Q

L

L

L

F

A

I

G

G

S

S

H

E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

T

V

V

A

S

F

I

K

L

L

C

L

P

P

P

V

K

P

P

binding flavin-adenine dinucleotide: P75 (≠ G25), G77 (≠ T27), G78 (≠ R28), N79 (≠ G29), T80 (≠ I30), G81 (= G31), M82 (≠ R32), G85 (= G35), S86 (≠ A36), L139 (= L89), G140 (= G90), A141 (≠ R91), C145 (≠ S95), G149 (= G99), N150 (≠ V100), A152 (= A102), T153 (≠ S103), A155 (= A105), G157 (= G107), G207 (= G157), I212 (≠ L162)

Sites not aligning to the query:

binding (2R)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 390, 423
binding flavin-adenine dinucleotide: 39, 422, 423
binding zinc ion: 381, 388, 422

6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
29% identity, 48% coverage: 1:173/360 of query aligns to 52:224/467 of 6lpnB

query
sites
6lpnB

M

L

R

R

P

P

G

R

T

T

E

S

Q

E

E

E

V

V

A

S

E

H

I

I

V

L

R

R

A

H

A

C

E

H

G

E

P

R

L

N

R

L

I

A

V

V

G

N

G
x
P

G

Q

T
x
G

R
x
G

G
x
N

I
x
T

G
|
G

R
x
M

C

V

A

G

G
|
G

A
x
S

V

V

-

P

-

V

-

F

-

D

-

E

-

I

L

L

E

S

T

T

C

A

G

R

L

M

A

N

G

R

I

V

R

L

L

S

Y

F

E

H

P

S

G

V

A

S

L

G

T

I

L

L

V

V

A

C

G

Q

A

A

G

G

T

C

R

V

L

L

A

E

D

E

V

L

E

S

A

R

A

Y

L

V

A

E

Q

R

G

D

Q

F

R

I

L

M

P

P

F

L

E

D

V

-

P

-

D

-

L

-

R

-

G

-

L

-

L
|
L

G

G

R
x
A

G

K

G

G

C

S

S
x
C

T
x
H

L

I

G

G

G
|
G

V
x
N

V

V

A
|
A

S
x
T

N

N

A

A

S

G

G

G

P

L

R

R

R

F

V

L

Q

R

A

Y

G

G

A

S

C

L

R

H

D

G

S

T

L

V

I

L

G

G

V

L

R

E

F

V

V

V

D

L

G

A

T

D

G

G

A

T

V

V

V

L

K

D

N

C

G

L

G

T

R

S

V

L

M

R

K

K

N

D

V

N

T

T

G

G

Y

Y

D

D

L

L

V

K

K

Q

L

L

L

F

A

I

G

G

S

S

H

E

G
|
G

T

T

L

L

G

G

V
x
I

L
x
I

T

T

E

T

V

V

A

S

F

I

K

L

L

C

L

P

P

P

V

K

P

P

binding flavin-adenine dinucleotide: P76 (≠ G25), G78 (≠ T27), G79 (≠ R28), N80 (≠ G29), T81 (≠ I30), G82 (= G31), M83 (≠ R32), G86 (= G35), S87 (≠ A36), L140 (= L89), A142 (≠ R91), C146 (≠ S95), H147 (≠ T96), G150 (= G99), N151 (≠ V100), A153 (= A102), T154 (≠ S103), G208 (= G157), I212 (≠ V161), I213 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 40, 423, 460

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/454 of 8jdvA

query
sites
8jdvA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding 2-Ketohexanoic acid: 75, 317, 321, 368, 375, 413
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 321, 322, 412, 413, 449
binding manganese (ii) ion: 368, 375, 412

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/455 of 8jdtA

query
sites
8jdtA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding 2-ketobutyric acid: 318, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 322, 413, 414, 450
binding manganese (ii) ion: 369, 376, 413

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/455 of 8jdeA

query
sites
8jdeA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 322, 413, 414, 450
binding lactic acid: 318, 369, 376, 414
binding manganese (ii) ion: 369, 376, 413

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/455 of 8jduA

query
sites
8jduA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding 2-oxopentanoic acid: 318, 322, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 322, 413, 450
binding manganese (ii) ion: 369, 376, 413

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/456 of 8jdsA

query
sites
8jdsA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 323, 414, 415, 451
binding manganese (ii) ion: 370, 377, 414
binding pyruvic acid: 319, 370, 377, 415

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/456 of 8jdrA

query
sites
8jdrA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 324, 370, 414, 451
binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: 319, 323, 415

8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/456 of 8jdqA

query
sites
8jdqA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding (2R)-2-hydroxy-4-methylpentanoic acid: 319, 323, 370, 415
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 370, 414, 451

8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/456 of 8jdoA

query
sites
8jdoA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding (2R)-2-hydroxyhexanoic acid: 319, 323, 415
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 324, 370, 414, 451

8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/456 of 8jdnA

query
sites
8jdnA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 370, 414, 451
binding (2R)-2-oxidanylpentanoic acid: 319, 323, 415

8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
31% identity, 41% coverage: 92:238/360 of query aligns to 133:273/456 of 8jdgA

query
sites
8jdgA

G
|
G

G
x
A

C

D

S

A

T

S

L

L

G

C

G
|
G

V
x
M

V

A

A
|
A

S
x
T

N

G

A
|
A

S
|
S

G

G

P

T

R

N

R

A

V

V

Q

R

A

Y

G

G

A

T

C

M

R

R

D

D

S

N

L

V

I

I

G

N

V

L

R

E

F

V

V

V

D

L

G

P

T

D

G

G

A

R

V

L

K

H

N

T

G

A

G

G

R

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

T

A

G

G

Y

Y

D

N

L

L

V

T

K

G

L

L

L

F

A

V

G

G

S

S

H
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

T

T

E

S

V

T

A

T

F

L

K

R

L

L

L

H

P

P

V

A

P

P

E

E

D

-

E

-

V

-

T

-

L

-

M

-

L

-

S

A

G

T

V

V

S

A

A

A

E

T

R

C

A

A

V

F

A

P

A

S

M

V

A

Q

A

A

L

V

G

D

S

S

P

T

F

V

E

Q

V

I

S

L

G

Q

A

A

H

V

W

P

P

V

G

A

R

R

G

I

T

E

F

F

L

L

R

-

I

-

E

D

G

D

F

V

A

M

S

D

V

A

A

C

Y

N

R

R

A

H

G

S

R

K

L

L

A

N

A

C

L

P

L

V

A

A

P

P

binding flavin-adenine dinucleotide: G133 (= G92), A134 (≠ G93), G140 (= G99), M141 (≠ V100), A143 (= A102), T144 (≠ S103), A146 (= A105), S147 (= S106), E200 (≠ H156), G201 (= G157), I206 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 132, 370, 414, 451
binding (2R)-2-oxidanylbutanoic acid: 319, 415

Query Sequence

>WP_097030568.1 NCBI__GCF_900207575.1:WP_097030568.1
MRPGTEQEVAEIVRAAEGPLRIVGGGTRGIGRCAGAVLETCGLAGIRLYEPGALTLVAGA
GTRLADVEAALAAQGQRLPFEVPDLRGLLGRGGCSTLGGVVASNASGPRRVQAGACRDSL
IGVRFVDGTGAVVKNGGRVMKNVTGYDLVKLLAGSHGTLGVLTEVAFKLLPVPEDEVTLM
LSGVSAERAVAAMAAALGSPFEVSGAAHWPGRGTFLRIEGFAASVAYRAGRLAALLAPFG
AVEQVAGPWRSIRDVEPFQGREGDVWRVLVKPSEAPGVAVRSGGEVLIDWGGGLVWVLVE
PGCDLRARIGPLAGHATLVRGGEAGDLPVFPPEPAAVAALSRGLRARFDPRGLFNPGLMA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory