SitesBLAST
Comparing WP_097031309.1 NCBI__GCF_900207575.1:WP_097031309.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
62% identity, 99% coverage: 1:443/448 of query aligns to 1:442/444 of 2vr1A
- active site: K116 (= K116), K159 (= K158), D194 (= D195), H207 (= H208), R233 (= R234), T272 (= T273), E274 (= E275), E286 (= E287), N288 (= N289), R290 (= R291), E294 (= E295), R336 (= R337)
- binding phosphodifluoromethylphosphonic acid-adenylate ester: K159 (= K158), R165 (= R166), M167 (= M168), Y201 (= Y202), L202 (= L203), E274 (= E275), L276 (= L277), E286 (= E287), N288 (= N289), I435 (= I436)
4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
61% identity, 99% coverage: 1:443/448 of query aligns to 1:441/442 of 4mv4A
- active site: K116 (= K116), K159 (= K158), D193 (= D195), H206 (= H208), R232 (= R234), T271 (= T273), E273 (= E275), E285 (= E287), N287 (= N289), R289 (= R291), E293 (= E295), R335 (= R337)
- binding phosphomethylphosphonic acid adenylate ester: K159 (= K158), G164 (= G163), M166 (= M168), E198 (= E200), Y200 (= Y202), L201 (= L203), H233 (= H235), L275 (= L277), E285 (= E287)
- binding magnesium ion: E273 (= E275), E285 (= E287)
4mv3A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and bicarbonate (see paper)
60% identity, 99% coverage: 1:443/448 of query aligns to 1:438/439 of 4mv3A
- active site: K116 (= K116), K159 (= K158), D190 (= D195), H203 (= H208), R229 (= R234), T268 (= T273), E270 (= E275), E282 (= E287), N284 (= N289), R286 (= R291), E290 (= E295), R332 (= R337)
- binding phosphomethylphosphonic acid adenylate ester: K159 (= K158), M163 (= M168), E195 (= E200), Y197 (= Y202), L198 (= L203), E270 (= E275), L272 (= L277), E282 (= E287)
- binding bicarbonate ion: R286 (= R291), Q288 (= Q293), V289 (= V294)
6oi8A Crystal structure of haemophilus influenzae biotin carboxylase complexed with 7-((1r,5s,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl)- 6-(2-chloro-6-(pyridin-3-yl)phenyl)pyrido[2,3-d]pyrimidin-2-amine (see paper)
60% identity, 99% coverage: 1:443/448 of query aligns to 1:439/440 of 6oi8A
- active site: K116 (= K116), K159 (= K158), D191 (= D195), H204 (= H208), R230 (= R234), T269 (= T273), E271 (= E275), E283 (= E287), N285 (= N289), R287 (= R291), E291 (= E295), R333 (= R337)
- binding 7-[(1R,5S,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl]-6-[2-chloro-6-(pyridin-3-yl)phenyl]pyrido[2,3-d]pyrimidin-2-amine: I157 (= I156), K159 (= K158), M164 (= M168), E196 (= E200), Y198 (= Y202), L199 (= L203), H204 (= H208), Q228 (= Q232), E271 (= E275), L273 (= L277), E283 (= E287), I432 (= I436)
P24182 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Escherichia coli (strain K12) (see 3 papers)
62% identity, 100% coverage: 1:446/448 of query aligns to 1:447/449 of P24182
- R19 (= R19) mutation to E: Loss of homodimerization. No effect on ATP binding.
- E23 (= E23) mutation to R: Loss of homodimerization. No effect on ATP binding.
- K116 (= K116) binding ATP
- K159 (= K158) binding ATP
- GG 165:166 (= GG 164:165) binding ATP
- EKYL 201:204 (= EKYL 200:203) binding ATP
- H209 (= H208) binding ATP
- H236 (= H235) binding ATP
- K238 (= K237) binding hydrogencarbonate
- E276 (= E275) binding ATP; binding Mg(2+)
- E288 (= E287) binding ATP; binding Mg(2+)
- R292 (= R291) active site; binding hydrogencarbonate
- V295 (= V294) binding hydrogencarbonate
- E296 (= E295) mutation to A: Severe reduction in catalytic activity.
- R338 (= R337) binding biotin; binding hydrogencarbonate; mutation to A: Severe reduction in catalytic activity.
- F363 (≠ L362) mutation to A: Loss of homodimerization. No effect on ATP binding.
- R366 (= R365) mutation to E: Loss of homodimerization. No effect on ATP binding.
P43873 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
61% identity, 99% coverage: 1:443/448 of query aligns to 1:444/448 of P43873
- K116 (= K116) binding ATP
- K159 (= K158) binding ATP
- EKYL 201:204 (= EKYL 200:203) binding ATP
- E276 (= E275) binding ATP; binding Mg(2+)
- E288 (= E287) binding ATP; binding Mg(2+)
- N290 (= N289) binding Mg(2+)
1dv2A The structure of biotin carboxylase, mutant e288k, complexed with atp (see paper)
62% identity, 100% coverage: 1:446/448 of query aligns to 4:450/450 of 1dv2A
- active site: K119 (= K116), K162 (= K158), D199 (= D195), H212 (= H208), R238 (= R234), T277 (= T273), E279 (= E275), K291 (≠ E287), N293 (= N289), R295 (= R291), E299 (= E295), R341 (= R337)
- binding adenosine-5'-triphosphate: K119 (= K116), K162 (= K158), G166 (= G162), G168 (= G164), M172 (= M168), E204 (= E200), K205 (= K201), Y206 (= Y202), L207 (= L203), H239 (= H235), L281 (= L277), K291 (≠ E287)
3jziA Crystal structure of biotin carboxylase from e. Coli in complex with benzimidazole series (see paper)
62% identity, 99% coverage: 1:443/448 of query aligns to 1:444/445 of 3jziA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E287), N290 (= N289), R292 (= R291), E296 (= E295), R338 (= R337)
- binding 7-amino-2-[(2-chlorobenzyl)amino]-1-{[(1S,2S)-2-hydroxycycloheptyl]methyl}-1H-benzimidazole-5-carboxamide: K116 (= K116), K159 (= K158), A160 (= A159), G164 (= G163), G165 (= G164), M169 (= M168), Y199 (= Y198), E201 (= E200), K202 (= K201), Y203 (= Y202), H209 (= H208), Q233 (= Q232), H236 (= H235), L278 (= L277), I287 (= I286), E288 (= E287)
2w6oA Crystal structure of biotin carboxylase from e. Coli in complex with 4-amino-7,7-dimethyl-7,8-dihydro-quinazolinone fragment (see paper)
62% identity, 99% coverage: 1:443/448 of query aligns to 1:444/445 of 2w6oA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E287), N290 (= N289), R292 (= R291), E296 (= E295), R338 (= R337)
- binding 4-amino-7,7-dimethyl-7,8-dihydroquinazolin-5(6H)-one: K159 (= K158), K202 (= K201), Y203 (= Y202), L204 (= L203), L278 (= L277), I437 (= I436)
2w6nA Crystal structure of biotin carboxylase from e. Coli in complex with amino-oxazole fragment series (see paper)
62% identity, 99% coverage: 1:443/448 of query aligns to 1:444/445 of 2w6nA