SitesBLAST

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
22% identity, 53% coverage: 174:386/402 of query aligns to 8:239/291 of 5odqB

query
sites
5odqB

G
|
G

C
|
C

V

I

Q

M

P

P

S

N

A

R

T

Y

P

A

N

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

T

E

M

L

V

V

E

D

A

M

P

T

E

G

A

A

G

S

C
|
C

G

P

A

A

V

P

N
x
G

Y

V

H

F

L

G

S

S

E

F

H

D

E

Q

K

K

G

T

-

W

L

L

E

T

R

L

M

A

R

A

Q

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

M

T

T

V

A
x
C

S

N

G
|
G

C
|
C

G

Y

A

G

M

S

V

L

Q

F

D

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

-

E

-

A

-

L

-

N

V

F

Y

V

A

N

A

E

K

K

A

L

Q

D

K

K

V

V

S

G

E

K

-

E

-

Y

-

K

-

G

-

N

-

V

-

K

-

V

-

R

-

H

-

F

-

A

-

E

-

L

L

I

C

Y

T

N

D

D

L

I

G

G

A

V

F

D

L

K

L

I

K

A

Q

E

D

K

L

V

E

E

L

-

L

R

K

P

V

L

R

N

Q

I

N

N

P

V

G

G

K

-

V

V

A

H

F

Y

H
x
G

C
|
C

-
x
H

-

F

-

L

-

K

P

P

C

T

T

D

L

V

Q

K

H

H

-

L

-

G

A

S

M

A

K

E

Q

R

N

P

G

V

V

M

V

L

E

D

Q

E

V

I

L

V

T

E

R

A

A

T

G

G

V

A

N

K

L

S

A

V

A

P

T

Y

K

A

D

D

K

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G

G

T

G

Y

V

S
x
R

V

A

T

R

Q

E

P

L

E

E

M

L

S

S

Q

L

K

D

L

M

L

T

G

N

N

E

K

K

L

I

K

E

A

N

L

M

T

I

I

K

D

A

N

G

P

A

D

D

R

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

M
x
L

H

Q

L

F

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), C41 (= C207), C42 (= C208), C78 (≠ A247), G80 (= G249), C81 (= C250), G152 (≠ H305), C153 (= C306), H154 (vs. gap), C193 (= C340), C194 (= C341), C231 (≠ N378), F233 (≠ G380), C234 (= C381)
binding 2-bromanylethanesulfonic acid: G46 (≠ N212), G80 (= G249), H154 (vs. gap), G197 (≠ A344), R201 (≠ S348), F233 (≠ G380), L236 (≠ M383)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
22% identity, 53% coverage: 174:386/402 of query aligns to 8:239/291 of 5odhH

query
sites
5odhH

G
|
G

C
|
C

V

I

Q

M

P

P

S

N

A

R

T

Y

P

A

N

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

T

E

M

L

V

V

E

D

A

M

P

T

E

G

A

A

G

S

C
|
C

G

P

A
|
A

V
x
P

N
x
G

Y

V

H

F

L

G

S

S

E

F

H

D

E

Q

K

K

G

T

-

W

L

L

E

T

R

L

M

A

R

A

Q

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

M

T

T

V

A
x
C

S

N

G
|
G

C
|
C

G

Y

A

G

M

S

V

L

Q

F

D

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

-

E

-

A

-

L

-

N

V

F

Y

V

A

N

A

E

K

K

A

L

Q

D

K

K

V

V

S

G

E

K

-

E

-

Y

-

K

-

G

-

N

-

V

-

K

-

V

-

R

-

H

-

F

-

A

-

E

-

L

L

I

C

Y

T

N

D

D

L

I

G

G

A

V

F

D

L

K

L

I

K

A

Q

E

D

K

L

V

E

E

L

-

L

R

K

P

V

L

R

N

Q

I

N

N

P

V

G

G

K

-

V

V

A

H

F

Y

H

G

C
|
C

-
x
H

-

F

-

L

-

K

P

P

C

T

T

D

L

V

Q

K

H

H

-

L

-

G

A

S

M

A

K

E

Q

R

N

P

G

V

V

M

V

L

E

D

Q

E

V

I

L

V

T

E

R

A

A

T

G

G

V

A

N

K

L

S

A

V

A

P

T

Y

K

A

D

D

K

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G
|
G

T

G

Y

V

S
x
R

V

A

T

R

Q

E

P

L

E

E

M

L

S

S

Q

L

K

D

L

M

L

T

G

N

N

E

K

K

L

I

K

E

A

N

L

M

T

I

I

K

D

A

N

G

P

A

D

D

R

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

M

L

H

Q

L

F

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), C41 (= C207), C42 (= C208), C78 (≠ A247), G80 (= G249), C81 (= C250), C153 (= C306), H154 (vs. gap), C193 (= C340), C194 (= C341), C231 (≠ N378), F233 (≠ G380), C234 (= C381)
binding 1-thioethanesulfonic acid: A44 (= A210), P45 (≠ V211), G46 (≠ N212), G80 (= G249), H154 (vs. gap), G197 (≠ A344), G198 (= G345)
binding Coenzyme B: R201 (≠ S348), F233 (≠ G380)

Sites not aligning to the query:

binding Coenzyme B: 240

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
22% identity, 53% coverage: 174:386/402 of query aligns to 8:239/291 of 5odhB

query
sites
5odhB

G
|
G

C
|
C

V
x
I

Q

M

P

P

S

N

A

R

T

Y

P

A

N

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

T

E

M

L

V

V

E

D

A

M

P

T

E

G

A

A

G

S

C
|
C

G

P

A

A

V
x
P

N
x
G

Y

V

H

F

L

G

S

S

E

F

H

D

E

Q

K

K

G

T

-

W

L

L

E

T

R

L

M

A

R

A

Q

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

M

T

T

V

A
x
C

S

N

G
|
G

C
|
C

G

Y

A

G

M

S

V

L

Q

F

D

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

-

E

-

A

-

L

-

N

V

F

Y

V

A

N

A

E

K

K

A

L

Q

D

K

K

V

V

S

G

E

K

-

E

-

Y

-

K

-

G

-

N

-

V

-

K

-

V

-

R

-

H

-

F

-

A

-

E

-

L

L

I

C

Y

T

N

D

D

L

I

G

G

A

V

F

D

L

K

L

I

K

A

Q

E

D

K

L

V

E

E

L

-

L

R

K

P

V

L

R

N

Q

I

N

N

P

V

G

G

K

-

V

V

A

H

F

Y

H
x
G

C
|
C

-
x
H

-

F

-

L

-

K

P

P

C

T

T

D

L

V

Q

K

H

H

-

L

-

G

A

S

M

A

K

E

Q

R

N

P

G

V

V

M

V

L

E

D

Q

E

V

I

L

V

T

E

R

A

A

T

G

G

V

A

N

K

L

S

A

V

A

P

T

Y

K

A

D

D

K

K

H

M

L

M

C
|
C

G

G

S

A

A

G

G

G

T

G

Y

V

S

R

V

A

T

R

Q

E

P

L

E

E

M

L

S

S

Q

L

K

D

L

M

L

T

G

N

N

E

K

K

L

I

K

E

A

N

L

M

T

I

I

K

D

A

N

G

P

A

D

D

R

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

M

L

H

Q

L

F

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), I10 (≠ V176), C41 (= C207), C42 (= C208), C78 (≠ A247), G80 (= G249), C81 (= C250), G152 (≠ H305), C153 (= C306), H154 (vs. gap), C193 (= C340), C194 (= C341), C231 (≠ N378), F233 (≠ G380), C234 (= C381)
binding 1-thioethanesulfonic acid: P45 (≠ V211), G46 (≠ N212), G80 (= G249), F233 (≠ G380)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
22% identity, 53% coverage: 174:386/402 of query aligns to 8:239/291 of 5odcB

query
sites
5odcB

G
|
G

C
|
C

V
x
I

Q

M

P

P

S

N

A

R

T

Y

P

A

N

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

T

E

M

L

V

V

E

D

A

M

P

T

E

G

A

A

G

S

C
|
C

G

P

A

A

V

P

N

G

Y

V

H

F

L

G

S

S

E

F

H

D

E

Q

K

K

G

T

-

W

L

L

E

T

R

L

M

A

R

A

Q

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

M

T

T

V

A
x
C

S
x
N

G
|
G

C
|
C

G

Y

A

G

M

S

V

L

Q

F

D

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

-

E

-

A

-

L

-

N

V

F

Y

V

A

N

A

E

K

K

A

L

Q

D

K

K

V

V

S

G

E

K

-

E

-

Y

-

K

-

G

-

N

-

V

-

K

-

V

-

R

-

H

-

F

-

A

-

E

-

L

L

I

C

Y

T

N

D

D

L

I

G

G

A

V

F

D

L

K

L

I

K

A

Q

E

D

K

L

V

E

E

L

-

L

R

K

P

V

L

R

N

Q

I

N

N

P

V

G

G

K

-

V

V

A

H

F

Y

H
x
G

C
|
C

-

H

-

F

-

L

-

K

P

P

C

T

T

D

L

V

Q

K

H

H

-

L

-

G

A

S

M

A

K

E

Q

R

N

P

G

V

V

M

V

L

E

D

Q

E

V

I

L

V

T

E

R

A

A

T

G

G

V

A

N

K

L

S

A

V

A

P

T

Y

K

A

D

D

K

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G

G

T

G

Y

V

S

R

V

A

T

R

Q

E

P

L

E

E

M

L

S

S

Q

L

K

D

L

M

L

T

G

N

N

E

K

K

L

I

K

E

A

N

L

M

T

I

I

K

D

A

N

G

P

A

D

D

R

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

M

L

H

Q

L

F

E

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), I10 (≠ V176), C41 (= C207), C42 (= C208), C78 (≠ A247), N79 (≠ S248), G80 (= G249), C81 (= C250), G152 (≠ H305), C153 (= C306), C193 (= C340), C194 (= C341), G197 (≠ A344), C231 (≠ N378), F233 (≠ G380), C234 (= C381)

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
25% identity, 24% coverage: 7:102/402 of query aligns to 168:270/280 of Q007T0

query
sites
Q007T0

Q

Q

F

Y

A

L

N

Q

T

S

A

I

E

E

G

E

Q

R

E

E

A

K

E

L

S

D

I

G

L

L

R

Y

A

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

E
x
W

L

N

N

G

D

D

E

K

R

Y

D

L

G

G

P

P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

M

W

F

M

L

I

E

D

G

S

A

R

E

D

V

D

T

F

E

T

K

E

T

E

R

R

-

L

-

A

-

K

-

L

-

Q

-

D

-

P

E

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C

I

R

M

S

N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

S

I

R

K

G

K

L

M

M

M

C186 (= C25) binding [4Fe-4S] cluster
C189 (= C28) binding [4Fe-4S] cluster
C192 (= C31) binding [4Fe-4S] cluster
C196 (= C35) binding [3Fe-4S] cluster
W201 (≠ E40) binding a ubiquinone
C243 (= C75) binding [3Fe-4S] cluster
C249 (= C81) binding [3Fe-4S] cluster
C253 (= C85) binding [4Fe-4S] cluster

Sites not aligning to the query:

93 binding [2Fe-2S] cluster
98 binding [2Fe-2S] cluster
101 binding [2Fe-2S] cluster
113 binding [2Fe-2S] cluster

8gs8B Cryo-em structure of the human respiratory complex ii (see paper)
25% identity, 24% coverage: 7:102/402 of query aligns to 134:236/239 of 8gs8B

query
sites
8gs8B

Q

Q

F

Y

A

L

N

Q

T

S

A

I

E

E

G

E

Q

R

E

E

A

K

E

L

S

D

I

G

L

L

R

Y

A

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

E
x
W

L

N

N

G

D

D

E

K

R
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

M

W

F

M

L

I

E

D

G

S

A

R

E

D

V

D

T

F

E

T

K

E

T

E

R

R

-

L

-

A

-

K

-

L

-

Q

-

D

-

P

E

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

S
x
N

C
|
C

E

T

T

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

S

I

R

K

G

K

L

M

M

M

binding fe3-s4 cluster: C162 (= C35), Y172 (≠ R45), P175 (= P48), C209 (= C75), H210 (≠ L76), T211 (= T77), I212 (≠ C78), M213 (≠ R79), N214 (≠ S80), C215 (= C81)
binding iron/sulfur cluster: C152 (= C25), C155 (= C28), C158 (= C31), A176 (≠ R49), C219 (= C85)
binding ubiquinone-1: P163 (= P36), W167 (≠ E40), I212 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 59, 60, 64, 65, 66, 67, 79

Query Sequence

>WP_099617495.1 NCBI__GCF_002744735.1:WP_099617495.1
MQTNLVQQFANTAEGQEAESILRACVHCGFCTATCPTYQELNDERDGPRGRIYLMKMFLE
GAEVTEKTREHLDRCLTCRSCETTCPSGVQYGRLVDISRGLMEKEMPRDPKDKWLRWTLA
RVIPNRQLFGLFLRLGQIFRPVLPEKLRTKVPPRKQANPWPAASHSRIVLALAGCVQPSA
TPNTNAAAARVLDRLGITMVEAPEAGCCGAVNYHLSEHEKGLERMRQNIDAWWPAIEAGA
EAIVMTASGCGAMVQDYGHLLKDDPVYAAKAQKVSELCTDLGAFLLKQDLELLKVRQNPG
KVAFHCPCTLQHAMKQNGVVEQVLTRAGVNLAATKDKHLCCGSAGTYSVTQPEMSQKLLG
NKLKALTIDNPDRIVTANIGCQMHLETRSPVPVQHWIELLDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory