SitesBLAST
Comparing WP_099617823.1 NCBI__GCF_002744735.1:WP_099617823.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 96% coverage: 8:247/251 of query aligns to 5:245/246 of 3osuA
5fffA Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and piperonal (see paper)
35% identity, 96% coverage: 5:246/251 of query aligns to 9:253/257 of 5fffA
- active site: K206 (= K196)
- binding 1,3-benzodioxole-5-carbaldehyde: Y100 (vs. gap), H158 (≠ S148)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G14), T20 (≠ S16), G22 (= G18), I23 (= I19), R43 (= R39), C67 (= C63), D68 (= D64), V69 (= V65), N96 (= N91), I146 (≠ L137), Y161 (= Y151), K165 (= K155), P191 (= P181), A193 (≠ S183), I194 (= I184), T196 (≠ F186), G198 (= G188), T199 (≠ G189)
5ff9B Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and tyramine (see paper)
35% identity, 96% coverage: 5:246/251 of query aligns to 9:253/257 of 5ff9B
- active site: K206 (= K196)
- binding 4-(2-aminoethyl)phenol: Y100 (vs. gap), I155 (≠ V145), H158 (≠ S148)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G14), T20 (≠ S16), K21 (= K17), I23 (= I19), S42 (≠ A38), R43 (= R39), C67 (= C63), D68 (= D64), V69 (= V65), N96 (= N91), I146 (≠ L137), S148 (= S139), Y161 (= Y151), K165 (= K155), P191 (= P181), A193 (≠ S183), I194 (= I184), T196 (≠ F186), G198 (= G188), T199 (≠ G189)
A0A1A9TAK5 Noroxomaritidine/norcraugsodine reductase; NorRed; EC 1.1.1.- from Narcissus pseudonarcissus (Daffodil) (see paper)
35% identity, 96% coverage: 5:246/251 of query aligns to 9:253/257 of A0A1A9TAK5
- TKGI 20:23 (≠ SKGI 16:19) binding NADP(+)
- SR 42:43 (≠ AR 38:39) binding NADP(+)
- DV 68:69 (= DV 64:65) binding NADP(+)
- NAG 96:98 (≠ N-- 91) binding NADP(+)
- Y100 (vs. gap) binding substrate
- C149 (≠ I140) binding substrate
- Y161 (= Y151) binding NADP(+)
- K165 (= K155) binding NADP(+)
- IRTPGT 194:199 (≠ IKFPGG 184:189) binding NADP(+)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
38% identity, 96% coverage: 8:247/251 of query aligns to 2:238/239 of 3sj7A
- active site: G12 (= G18), S138 (= S139), Q148 (= Q149), Y151 (= Y151), K155 (= K155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), S10 (= S16), R11 (≠ K17), I13 (= I19), N31 (vs. gap), Y32 (≠ C37), A33 (= A38), G34 (≠ R39), S35 (= S40), A58 (≠ C63), N59 (≠ D64), V60 (= V65), N86 (= N91), A87 (≠ S93), T109 (≠ L110), S138 (= S139), Y151 (= Y151), K155 (= K155), P181 (= P181), G182 (= G182)
3ai2A The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH (see paper)
34% identity, 99% coverage: 1:249/251 of query aligns to 1:262/263 of 3ai2A
- active site: G18 (= G18), S144 (= S139), Y157 (= Y151), K161 (= K155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G14), S16 (= S16), S17 (≠ K17), G18 (= G18), I19 (= I19), A38 (= A38), R39 (= R39), Q40 (≠ S40), V64 (≠ C63), D65 (= D64), V66 (= V65), N92 (= N91), G94 (= G96), N142 (≠ L137), Y157 (= Y151), K161 (= K155), P187 (= P181), I190 (= I184), T192 (≠ F186), W195 (vs. gap)
3ai3C The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH and l-sorbose (see paper)
34% identity, 99% coverage: 1:249/251 of query aligns to 1:262/263 of 3ai3C
- active site: G18 (= G18), S144 (= S139), Y157 (= Y151), K161 (= K155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G14), S16 (= S16), S17 (≠ K17), G18 (= G18), I19 (= I19), A38 (= A38), R39 (= R39), Q40 (≠ S40), R43 (≠ P43), D65 (= D64), V66 (= V65), N92 (= N91), G94 (= G96), N142 (≠ L137), Y157 (= Y151), K161 (= K155), P187 (= P181), G188 (= G182), I190 (= I184), T192 (≠ F186), W195 (vs. gap)
- binding alpha-L-sorbopyranose: A252 (≠ C239), F254 (≠ P241)
- binding L-sorbose: G96 (= G98), E154 (≠ Q149), Y157 (= Y151), W195 (vs. gap)
Sites not aligning to the query:
3ai3A The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH and l-sorbose (see paper)
34% identity, 99% coverage: 1:249/251 of query aligns to 1:262/263 of 3ai3A
- active site: G18 (= G18), S144 (= S139), Y157 (= Y151), K161 (= K155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G14), S16 (= S16), S17 (≠ K17), G18 (= G18), I19 (= I19), A38 (= A38), R39 (= R39), Q40 (≠ S40), R43 (≠ P43), V64 (≠ C63), D65 (= D64), V66 (= V65), N92 (= N91), G94 (= G96), N142 (≠ L137), S144 (= S139), Y157 (= Y151), K161 (= K155), P187 (= P181), G188 (= G182), I190 (= I184), T192 (≠ F186), W195 (vs. gap)
- binding L-sorbose: G96 (= G98), S144 (= S139), L151 (≠ G146), E154 (≠ Q149), Y157 (= Y151), G188 (= G182)
3toxA Crystal structure of a short chain dehydrogenase in complex with NAD(p) from sinorhizobium meliloti 1021
36% identity, 96% coverage: 5:244/251 of query aligns to 3:248/254 of 3toxA
- active site: G16 (= G18), S142 (= S139), V153 (vs. gap), Y156 (= Y151), K160 (= K155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (= S16), S15 (≠ K17), G16 (= G18), I17 (= I19), A36 (= A38), R37 (= R39), N38 (≠ S40), V63 (= V65), N89 (= N91), A90 (≠ V92), G91 (≠ S93), T140 (≠ L137), S142 (= S139), Y156 (= Y151), K160 (= K155), P186 (= P181), G188 (≠ S183), T189 (≠ I184), T191 (≠ F186)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
36% identity, 98% coverage: 3:247/251 of query aligns to 1:242/244 of 6t77A
- active site: G16 (= G18), S138 (= S139), Y151 (= Y151)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (= S16), R15 (≠ K17), T37 (≠ R39), L58 (≠ C63), N59 (≠ D64), V60 (= V65), A87 (≠ V92), G88 (≠ S93), I89 (≠ A94)
Q9ZW19 Tropinone reductase homolog At2g29360; EC 1.1.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 96% coverage: 5:244/251 of query aligns to 16:258/271 of Q9ZW19
- S209 (≠ K196) mutation to Y: Loss of activity with quinuclidinone and decreased activity with cyclohexanones.
3pk0B Crystal structure of short-chain dehydrogenase/reductase sdr from mycobacterium smegmatis (see paper)
33% identity, 96% coverage: 5:246/251 of query aligns to 8:251/262 of 3pk0B
P50162 Tropinone reductase 1; Tropine dehydrogenase; Tropinone reductase I; TR-I; EC 1.1.1.206 from Datura stramonium (Jimsonweed) (Common thornapple) (see paper)
35% identity, 96% coverage: 5:244/251 of query aligns to 19:265/273 of P50162
- 25:49 (vs. 11:35, 56% identical) binding NADP(+)
- S158 (= S139) binding substrate
- Y171 (= Y151) active site, Proton acceptor
A7DY56 Tropinone reductase; EC 1.1.1.206; EC 1.1.1.236 from Cochlearia officinalis (Common scurvygrass) (see paper)
33% identity, 96% coverage: 5:244/251 of query aligns to 16:258/273 of A7DY56
- Y209 (≠ R201) mutation to S: Loss of tropinone or nortropinone reduction, but faster reduction of cyclohexanones.
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 98% coverage: 3:247/251 of query aligns to 1:242/244 of P0AEK2
- GASR 12:15 (≠ GGSK 14:17) binding NADP(+)
- T37 (≠ R39) binding NADP(+)
- NV 59:60 (≠ DV 64:65) binding NADP(+)
- N86 (= N91) binding NADP(+)
- Y151 (= Y151) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YAATK 151:155) binding NADP(+)
- A154 (≠ T154) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K155) mutation to A: Defect in the affinity for NADPH.
- I184 (= I184) binding NADP(+)
- E233 (≠ A238) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 98% coverage: 3:247/251 of query aligns to 1:242/244 of P0A2C9
- M125 (= M126) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A228) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S229) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
1ae1B Tropinone reductase-i complex with NADP (see paper)
35% identity, 96% coverage: 5:244/251 of query aligns to 4:250/258 of 1ae1B