SitesBLAST
Comparing WP_099617893.1 NCBI__GCF_002744735.1:WP_099617893.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
25% identity, 54% coverage: 8:515/938 of query aligns to 11:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ F69), G75 (≠ A71), S76 (≠ A72), G77 (= G73), T78 (= T74), G79 (≠ S75), L80 (= L76), A83 (≠ Q79), C84 (≠ A80), P137 (= P131), G138 (≠ A132), E139 (≠ S133), A142 (= A136), T143 (≠ C137), G146 (= G141), N147 (≠ I142), S149 (≠ A144), T150 (≠ N145), A152 (= A147), G153 (≠ S148), E203 (= E249), G204 (= G250), I209 (≠ V255), E422 (= E474), H423 (= H475)
- binding fe (iii) ion: H377 (= H429), H384 (= H436), E422 (= E474)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
27% identity, 50% coverage: 52:518/938 of query aligns to 51:458/459 of P9WIT1
- K354 (≠ E407) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
22% identity, 53% coverage: 12:512/938 of query aligns to 49:513/521 of Q8N465
- S109 (≠ E50) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ T68) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (≠ A72) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ L86) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ Q92) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V111) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ G128) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A144) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ V170) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G172) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (= D365) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R376) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ F380) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (= V393) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ I395) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ Q413) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ G420) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H429) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (≠ L431) to V: slight reduction in catalytic activity
- N439 (= N434) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H436) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (≠ V438) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ F439) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ Q441) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E474) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H475) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G476) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
23% identity, 53% coverage: 13:512/938 of query aligns to 18:460/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R376), T337 (≠ F380), K348 (≠ I395), Y379 (≠ F427), H381 (= H429), H388 (= H436), H423 (= H475)
- binding flavin-adenine dinucleotide: W39 (vs. gap), P75 (≠ F69), Q76 (≠ R70), G77 (≠ A71), G78 (≠ A72), N79 (≠ G73), T80 (= T74), G81 (≠ S75), M82 (≠ L76), G85 (≠ Q79), S86 (≠ A80), L139 (≠ P131), G140 (≠ A132), A141 (≠ S133), C145 (= C137), G149 (= G141), N150 (≠ I142), A152 (= A144), T153 (≠ N145), G157 (= G149), G207 (= G250), I212 (≠ V255), E422 (= E474), N459 (= N511)
- binding zinc ion: H381 (= H429), H388 (= H436), E422 (= E474)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
23% identity, 53% coverage: 13:512/938 of query aligns to 18:460/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (vs. gap), P75 (≠ F69), G77 (≠ A71), G78 (≠ A72), N79 (≠ G73), T80 (= T74), G81 (≠ S75), G85 (≠ Q79), S86 (≠ A80), L139 (≠ P131), G140 (≠ A132), A141 (≠ S133), C145 (= C137), H146 (≠ K138), G148 (= G140), G149 (= G141), N150 (≠ I142), A152 (= A144), T153 (≠ N145), A155 (= A147), E206 (= E249), G207 (= G250), I211 (≠ F254), I212 (≠ V255), E422 (= E474), N459 (= N511)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R376), T337 (≠ F380), K348 (≠ I395), Y379 (≠ F427), H381 (= H429), H388 (= H436), H423 (= H475)
- binding zinc ion: H381 (= H429), H388 (= H436), E422 (= E474)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
23% identity, 53% coverage: 13:512/938 of query aligns to 18:460/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (vs. gap), P75 (≠ F69), G77 (≠ A71), G78 (≠ A72), N79 (≠ G73), T80 (= T74), G81 (≠ S75), G85 (≠ Q79), S86 (≠ A80), L139 (≠ P131), G140 (≠ A132), A141 (≠ S133), C145 (= C137), H146 (≠ K138), G149 (= G141), N150 (≠ I142), A152 (= A144), T153 (≠ N145), A155 (= A147), G157 (= G149), E206 (= E249), G207 (= G250), I211 (≠ F254), I212 (≠ V255), E422 (= E474), N459 (= N511)
- binding d-malate: M82 (≠ L76), R333 (= R376), T337 (≠ F380), K348 (≠ I395), Y379 (≠ F427), H381 (= H429), H388 (= H436), E422 (= E474), H423 (= H475)
- binding zinc ion: H381 (= H429), H388 (= H436), E422 (= E474)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
23% identity, 53% coverage: 13:512/938 of query aligns to 18:460/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R376), T337 (≠ F380), K348 (≠ I395), Y379 (≠ F427), H381 (= H429), H388 (= H436), N390 (≠ V438), H423 (= H475)
- binding flavin-adenine dinucleotide: W39 (vs. gap), P75 (≠ F69), G77 (≠ A71), G78 (≠ A72), N79 (≠ G73), T80 (= T74), G81 (≠ S75), M82 (≠ L76), G85 (≠ Q79), S86 (≠ A80), L139 (≠ P131), G140 (≠ A132), A141 (≠ S133), C145 (= C137), G149 (= G141), N150 (≠ I142), A152 (= A144), T153 (≠ N145), A155 (= A147), G157 (= G149), G207 (= G250), I212 (≠ V255), E422 (= E474), H423 (= H475)
- binding zinc ion: H381 (= H429), H388 (= H436), E422 (= E474)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
23% identity, 53% coverage: 13:512/938 of query aligns to 19:461/467 of 6lpnB