SitesBLAST
Comparing WP_099618892.1 NCBI__GCF_002744735.1:WP_099618892.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
67% identity, 98% coverage: 7:395/396 of query aligns to 4:391/392 of P33221
- EL 22:23 (= EL 25:26) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E85) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R117) binding ATP
- K155 (= K158) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 163:168) binding ATP
- G162 (= G165) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ E182) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFV 198:201) binding ATP
- E203 (= E206) binding ATP
- E267 (= E270) binding Mg(2+)
- E279 (= E282) binding Mg(2+)
- D286 (= D289) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K359) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 366:367) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
67% identity, 98% coverage: 7:395/396 of query aligns to 3:388/389 of 1kjiA
- active site: E114 (= E118), K154 (= K158), S159 (= S163), G161 (= G165), E264 (= E270), E276 (= E282), D283 (= D289), T284 (= T290), R360 (= R367)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R117), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (= Q168), E192 (= E198), V195 (= V201), E200 (= E206), Q222 (= Q228), E264 (= E270), F266 (= F272), E276 (= E282)
- binding magnesium ion: E264 (= E270), E276 (= E282)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
67% identity, 98% coverage: 7:395/396 of query aligns to 3:388/389 of 1ez1A
- active site: E114 (= E118), K154 (= K158), S159 (= S163), G161 (= G165), E264 (= E270), E276 (= E282), D283 (= D289), T284 (= T290), R360 (= R367)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R117), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), E192 (= E198), V194 (≠ F200), V195 (= V201), F197 (= F203), E200 (= E206), Q222 (= Q228), E264 (= E270), F266 (= F272), E276 (= E282)
- binding glycinamide ribonucleotide: G20 (= G24), E21 (= E25), L22 (= L26), E81 (= E85), I82 (= I86), S160 (= S164), D283 (= D289), K352 (= K359), R359 (= R366), R360 (= R367)
- binding magnesium ion: E264 (= E270), E276 (= E282)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
67% identity, 98% coverage: 7:395/396 of query aligns to 3:388/389 of 1eyzA
- active site: E114 (= E118), K154 (= K158), S159 (= S163), G161 (= G165), E264 (= E270), E276 (= E282), D283 (= D289), T284 (= T290), R360 (= R367)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R117), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (= Q168), E192 (= E198), V195 (= V201), F197 (= F203), E200 (= E206), E264 (= E270), F266 (= F272), E276 (= E282)
- binding magnesium ion: E264 (= E270), E276 (= E282)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
67% identity, 98% coverage: 7:395/396 of query aligns to 3:387/388 of 1kjqA
- active site: E114 (= E118), K154 (= K158), E263 (= E270), E275 (= E282), D282 (= D289), T283 (= T290), R359 (= R367)
- binding adenosine-5'-diphosphate: R113 (= R117), I152 (≠ V156), K154 (= K158), E191 (= E198), V193 (≠ F200), V194 (= V201), F196 (= F203), E199 (= E206), Q221 (= Q228), F265 (= F272), E275 (= E282)
- binding magnesium ion: E263 (= E270), E275 (= E282)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
66% identity, 98% coverage: 7:395/396 of query aligns to 3:385/386 of 1kjjA
- active site: E114 (= E118), K154 (= K158), S159 (= S163), G161 (= G165), E261 (= E270), E273 (= E282), D280 (= D289), T281 (= T290), R357 (= R367)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R117), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (= Q168), E189 (= E198), V192 (= V201), E197 (= E206), Q219 (= Q228), E261 (= E270), F263 (= F272), E273 (= E282)
- binding magnesium ion: E261 (= E270), E273 (= E282)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
66% identity, 98% coverage: 7:395/396 of query aligns to 3:385/386 of 1kj8A
- active site: E114 (= E118), K154 (= K158), S159 (= S163), G161 (= G165), E261 (= E270), E273 (= E282), D280 (= D289), T281 (= T290), R357 (= R367)
- binding adenosine-5'-triphosphate: R113 (= R117), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (= Q168), E189 (= E198), V192 (= V201), F194 (= F203), E197 (= E206), Q219 (= Q228), G222 (= G231), E261 (= E270), F263 (= F272), E273 (= E282)
- binding glycinamide ribonucleotide: G20 (= G24), E21 (= E25), L22 (= L26), E81 (= E85), I82 (= I86), S160 (= S164), D280 (= D289), K349 (= K359), R356 (= R366)
- binding magnesium ion: E261 (= E270), E273 (= E282)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
47% identity, 98% coverage: 7:394/396 of query aligns to 8:410/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
46% identity, 98% coverage: 7:394/396 of query aligns to 10:399/409 of 2dwcB
- active site: E265 (= E270), E277 (= E282), D284 (= D289), T285 (= T290), R372 (= R367)
- binding adenosine-5'-diphosphate: R120 (= R117), H159 (≠ V156), K161 (= K158), H190 (≠ F200), I191 (≠ V201), F193 (= F203), E196 (= E206), F267 (= F272), E277 (= E282)
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 92% coverage: 30:394/396 of query aligns to 16:357/360 of 3ax6A
- active site: E231 (= E270), E244 (= E282), N251 (≠ D289), S252 (≠ T290), K330 (≠ R367)
- binding adenosine-5'-diphosphate: K101 (≠ R117), V136 (= V156), K138 (= K158), E164 (= E198), F166 (= F200), V167 (= V201), E172 (= E206), F233 (= F272), N243 (≠ S281)
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 92% coverage: 25:387/396 of query aligns to 19:369/381 of 3v4sB
- binding adenosine-5'-triphosphate: R108 (= R117), K148 (= K158), Y154 (vs. gap), D155 (≠ S164), G156 (= G165), Q159 (= Q168), E183 (= E198), W185 (≠ F200), V186 (= V201), F188 (= F203), E191 (= E206), H214 (≠ Q228), N217 (≠ G231), E256 (= E270), F258 (= F272), E269 (= E282)
- binding carbonate ion: R273 (= R286), H275 (= H288), N276 (≠ D289)
- binding magnesium ion: T105 (= T114), E111 (≠ R121), E256 (= E270), E269 (= E282), L270 (≠ V283)
3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 92% coverage: 25:387/396 of query aligns to 18:368/377 of 3q2oB