SitesBLAST
Comparing WP_100847701.1 NCBI__GCF_003031005.1:WP_100847701.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
31% identity, 99% coverage: 1:305/309 of query aligns to 1:304/306 of 4xckA
- active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
- binding adenosine-5'-diphosphate: T220 (≠ R222), G222 (= G224), S223 (≠ P225), V242 (= V244), T247 (≠ S249), A250 (= A252), F254 (≠ H256), H276 (≠ N278), A279 (= A281), V283 (= V285)
- binding alpha-D-ribofuranose: N11 (≠ I11), D13 (= D13), G39 (= G39), K40 (vs. gap), N43 (= N42), A95 (≠ C93), I107 (≠ F105), I109 (≠ S107), E140 (≠ Q145), T248 (≠ N250), D252 (= D254)
8cqxA Ribokinase from t.Sp mutant a92g
32% identity, 97% coverage: 4:303/309 of query aligns to 2:298/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N189), T217 (≠ R222), G219 (= G224), A220 (≠ P225), G222 (= G227), F250 (≠ H256), N272 (= N278), G275 (≠ A281), A276 (= A282), T279 (≠ V285)
- binding magnesium ion: D242 (= D248), T244 (≠ N250), A278 (= A284), S287 (= S293)
6a8cA Ribokinase from leishmania donovani with adp (see paper)
29% identity, 97% coverage: 2:301/309 of query aligns to 14:324/327 of 6a8cA
- binding adenosine-5'-diphosphate: G245 (= G224), A246 (≠ P225), T271 (≠ S249), A274 (= A252), G275 (= G253), N300 (= N278), A303 (= A281)
- binding glycerol: D25 (= D13), S42 (≠ A30), S44 (= S32), G50 (≠ A37), G51 (= G38), N55 (= N42)
6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
29% identity, 97% coverage: 2:301/309 of query aligns to 14:324/327 of 6a8bA
- binding phosphomethylphosphonic acid adenylate ester: G245 (= G224), A246 (≠ P225), T271 (≠ S249), A274 (= A252), G275 (= G253), N300 (= N278), A303 (= A281), V307 (= V285)
- binding glycerol: D25 (= D13), G50 (≠ A37), G51 (= G38), N55 (= N42), N157 (≠ W150), I159 (≠ L152), E190 (≠ D172)
6a8aA Ribokinase from leishmania donovani with atp (see paper)
29% identity, 97% coverage: 2:301/309 of query aligns to 14:324/327 of 6a8aA
- binding adenosine-5'-triphosphate: N207 (= N189), T243 (≠ V220), G245 (= G224), A246 (≠ P225), G248 (= G227), T271 (≠ S249), G273 (= G251), A274 (= A252), G275 (= G253), N300 (= N278), A303 (= A281), V307 (= V285)
- binding glycerol: D25 (= D13), G50 (≠ A37), G51 (= G38), N55 (= N42)
6znxC Ribokinase from thermus species
32% identity, 86% coverage: 38:303/309 of query aligns to 24:263/265 of 6znxC
3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
28% identity, 78% coverage: 49:290/309 of query aligns to 47:291/308 of 3iq0B
- active site: G252 (= G251), A253 (= A252), G254 (= G253), D255 (= D254)
- binding adenosine-5'-triphosphate: S192 (≠ N189), K223 (≠ R222), G225 (= G224), E247 (≠ A246), A253 (= A252), G254 (= G253), F257 (≠ H256), N279 (= N278), G282 (≠ A281)
3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
27% identity, 83% coverage: 38:292/309 of query aligns to 40:296/312 of 3in1A
- active site: R106 (= R103), G255 (= G251), A256 (= A252), G257 (= G253), D258 (= D254)
- binding adenosine-5'-diphosphate: N194 (= N189), K225 (≠ R222), G227 (= G224), G230 (= G227), A244 (≠ G241), T253 (≠ S249), N282 (= N278), A285 (= A281)
1gqtB Activation of ribokinase by monovalent cations (see paper)
28% identity, 96% coverage: 8:305/309 of query aligns to 10:306/307 of 1gqtB
- active site: A251 (≠ G251), A252 (= A252), G253 (= G253), D254 (= D254)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N189), T222 (≠ V220), G224 (= G224), S225 (≠ P225), A252 (= A252), G253 (= G253), H278 (≠ N278), A281 (= A281)
- binding cesium ion: D248 (= D248), I250 (≠ N250), A284 (= A284), R287 (= R287), S293 (= S293)
- binding alpha-D-ribofuranose: N13 (≠ I11), D15 (= D13), G41 (= G39), N45 (= N42), E142 (≠ K143), D254 (= D254)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 96% coverage: 8:305/309 of query aligns to 11:307/309 of P0A9J6
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
28% identity, 96% coverage: 8:305/309 of query aligns to 8:304/305 of 1rk2A
- active site: A249 (≠ G251), A250 (= A252), G251 (= G253), D252 (= D254)
- binding adenosine-5'-diphosphate: T220 (≠ V220), G222 (= G224), S223 (≠ P225), A250 (= A252), G251 (= G253), H276 (≠ N278), A279 (= A281)
- binding tetrafluoroaluminate ion: G213 (≠ L213), R215 (≠ A215)
- binding magnesium ion: D246 (= D248), A282 (= A284), R285 (= R287), S291 (= S293)
- binding alpha-D-ribofuranose: N11 (≠ I11), D13 (= D13), G38 (= G38), G39 (= G39), K40 (vs. gap), N43 (= N42), E140 (≠ K143), D252 (= D254)
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
26% identity, 93% coverage: 2:289/309 of query aligns to 3:294/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N189), K225 (≠ R222), G227 (= G224), I246 (≠ V239), A248 (= A243), A257 (= A252), G258 (= G253), F261 (≠ H256), A286 (= A281), S287 (≠ A282)
- binding alpha-D-ribofuranose: N12 (≠ I11), D14 (= D13), G40 (= G39), K41 (vs. gap), N44 (= N42), E144 (≠ G142), D259 (= D254)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 93% coverage: 2:289/309 of query aligns to 69:360/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
28% identity, 87% coverage: 38:306/309 of query aligns to 34:304/309 of Q53W83
- GAEVN 34:38 (≠ GGGFN 38:42) binding substrate
- YYR 103:105 (≠ ISY 106:108) binding substrate
- R167 (vs. gap) binding substrate
- S193 (≠ N189) binding ATP
- 219:225 (vs. 222:228, 29% identical) binding ATP
- GAGD 248:251 (= GAGD 251:254) binding ATP
- D251 (= D254) binding substrate
- N275 (= N278) binding ATP
- D287 (≠ G289) binding substrate
5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
26% identity, 85% coverage: 42:303/309 of query aligns to 32:305/306 of 5eynA