SitesBLAST

P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
33% identity, 37% coverage: 44:173/349 of query aligns to 258:385/658 of P97275

query
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P97275

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H300 (≠ C86) mutation to A: Loss of activity.
303:309 (vs. 89:96, 25% identical) binding FAD
T309 (= T96) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
TRAS 316:319 (≠ TGLS 103:106) binding FAD
S367 (= S155) mutation to A: Strongly reduced activity.
368:374 (vs. 156:162, 57% identical) binding FAD

Sites not aligning to the query:

1:58 modified: transit peptide, Peroxisome
234:240 binding FAD
419 Important for enzyme activity; R→H: Loss of activity.; R→K: Strongly reduced activity.
469 L→P: Impaired FAD binding and protein stability. Loss of activity.
515 binding substrate; R→L: Impaired FAD binding and protein stability. Loss of activity.
576 C→A: No effect on activity.
578 active site, Proton donor/acceptor; Y→F: Loss of activity.
615 H→A: Loss of activity.
615:617 Important for enzyme activity
616 H→A: Loss of activity.
617 H→A: Loss of activity.
654:658 Important for enzyme activity

4bc9B Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
33% identity, 38% coverage: 44:174/349 of query aligns to 164:292/542 of 4bc9B

query
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4bc9B

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binding propanenitrile: D209 (≠ P89)
binding flavin-adenine dinucleotide: P208 (= P88), D209 (≠ P89), S210 (≠ A90), S214 (≠ A95), T215 (= T96), G218 (= G99), W219 (≠ M100), S221 (≠ A102), T222 (= T103), A224 (≠ L105), S225 (= S106), E274 (≠ Y156), G275 (= G157), I280 (≠ L162)

Sites not aligning to the query:

binding propanenitrile: 462, 501
binding flavin-adenine dinucleotide: 140, 142, 143, 144, 145, 146, 147, 150, 151, 500

5ae2B Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
33% identity, 38% coverage: 44:174/349 of query aligns to 164:292/542 of 5ae2B

query
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5ae2B

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binding flavin-adenine dinucleotide: P208 (= P88), D209 (≠ P89), S210 (≠ A90), S214 (≠ A95), T215 (= T96), G218 (= G99), W219 (≠ M100), S221 (≠ A102), T222 (= T103), A224 (≠ L105), S225 (= S106), E274 (≠ Y156), G275 (= G157), V279 (= V161), I280 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 16, 140, 142, 143, 144, 145, 146, 150, 151, 500
binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 399, 409, 410, 411, 462, 464, 466, 500, 501

5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
33% identity, 38% coverage: 44:174/349 of query aligns to 169:297/561 of 5ae2A

query
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5ae2A

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binding flavin-adenine dinucleotide: P213 (= P88), D214 (≠ P89), S215 (≠ A90), S219 (≠ A95), T220 (= T96), G223 (= G99), W224 (≠ M100), S226 (≠ A102), T227 (= T103), A229 (≠ L105), S230 (= S106), E279 (≠ Y156), V284 (= V161), I285 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 16, 145, 146, 147, 148, 149, 150, 151, 155, 156, 519
binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 415, 418, 428, 429, 430, 481, 483, 519

5ae1A Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
33% identity, 38% coverage: 44:174/349 of query aligns to 163:291/560 of 5ae1A

query
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5ae1A

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binding flavin-adenine dinucleotide: P207 (= P88), D208 (≠ P89), S209 (≠ A90), S213 (≠ A95), T214 (= T96), G216 (= G98), G217 (= G99), W218 (≠ M100), S220 (≠ A102), T221 (= T103), A223 (≠ L105), S224 (= S106), E273 (≠ Y156), G274 (= G157), V278 (= V161), I279 (≠ L162)

Sites not aligning to the query:

binding (3-(2-fluorophenyl)-n-(1-(2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5-yl)ethyl)butanamide): 416, 417, 427, 428, 429, 480, 518
binding flavin-adenine dinucleotide: 139, 140, 141, 142, 143, 144, 145, 146, 149, 150, 518

5ae1B Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
33% identity, 37% coverage: 44:173/349 of query aligns to 164:291/539 of 5ae1B

query
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5ae1B

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binding flavin-adenine dinucleotide: P208 (= P88), D209 (≠ P89), S210 (≠ A90), S214 (≠ A95), T215 (= T96), G218 (= G99), W219 (≠ M100), S221 (≠ A102), T222 (= T103), A224 (≠ L105), S225 (= S106), E274 (≠ Y156), V279 (= V161), I280 (≠ L162)

Sites not aligning to the query:

binding (3-(2-fluorophenyl)-n-(1-(2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5-yl)ethyl)butanamide): 392, 396, 406, 407, 408, 459, 497
binding flavin-adenine dinucleotide: 16, 140, 141, 142, 143, 144, 145, 146, 150, 151, 497, 535

5adzA Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
33% identity, 37% coverage: 44:173/349 of query aligns to 166:293/566 of 5adzA

query
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5adzA

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binding flavin-adenine dinucleotide: P210 (= P88), D211 (≠ P89), S212 (≠ A90), S216 (≠ A95), T217 (= T96), G220 (= G99), W221 (≠ M100), S223 (≠ A102), T224 (= T103), A226 (≠ L105), S227 (= S106), E276 (≠ Y156), G277 (= G157), V281 (= V161), I282 (≠ L162)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D211 (≠ P89)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 142, 143, 144, 145, 146, 147, 148, 152, 524
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): 423, 433, 434, 435, 486, 488, 489, 524, 525

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 42% coverage: 52:197/349 of query aligns to 96:230/459 of P9WIT1

query
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P9WIT1

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R

L

Y

P

T

C

V

E

Y

P

P

P

-

A

-

F

-

G

G

E

E

-

L

G

S

A

S

T

S

V

V

G

G

M

N

I

V

A

G

T

T

G

N

L

A

S

G

G

G

P

M

R

R

R

A

P

V

W

K

S

Y

G

G

S

V

V

A

R

R

D

H

F

N

V

V

L

L

G

G

T

L

R

Q

V

A

I

V

T

L

G

P

L

T

G

G

Q

E

H

I

L

I

R

R

F

T

G

G

E

R

V

M

M

A

K

K

N

V

V

S

A

T

G

G

Y

Y

D

D

L

L

S

T

R

Q

L

L

M

I

A

I

G

G

S

S

Y

E

G

G

C

T

L

L

G

A

V

L

L

V

T

T

E

E

V

V

S

I

L

V

K

K

V

L

L

H

P

P

K

-

P

-

R

-

Q

-

C

-

L

-

S

-

I

-

R

R

L

L

D

D

I

H

D

N

C

A

T

S

R

-

A

V

L

L

A

A

N

P

L

F

A

A

E

D

W

F

G

D

Q

Q

Sites not aligning to the query:

354 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
31% identity, 42% coverage: 27:174/349 of query aligns to 76:224/466 of 6lpxA

query
sites
6lpxA

Q
|
Q

G
|
G

N
|
N

S
x
T

K
x
G

A
x
M

F

V

L

G

G
|
G

R
x
S

E

V

V

P

A

V

G

F

E

D

-

E

-

I

V

I

L

L

D

S

T

T

R

A

A

R

H

M

R

N

G

R

I

V

V

L

R

S

Y

F

D

H

P

S

T

V

E

S

L

G

V

I

V

L

T

V

V

C

R

Q

A

A

G

G

T

C

P

V

L

L

S

E

E

E

L

L

L

S

A

R

A

Y

L

V

D

E

A

E

A

R

G

D

Q

F

M

I

L

M

P

P

C

L

E

D

P
x
L

P
x
G

A
|
A

F

K

G

G

E

S

G

-

A
x
C

T

H

V

I

G

G

G
|
G

M
x
N

I

V

A
|
A

T
|
T

G

N

L

A

S

G

G
|
G

P

L

R

R

R

F

P

L

W

R

S

Y

G

G

S

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

T

L

R

E

V

V

I

V

T

L

G

A

L

D

G

G

Q

T

H

V

L

L

R

D

F

C

G

L

G

T

E

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

L

L

S

K

R

Q

L

L

M

F

A

I

G

G

S

S

Y

E

G
|
G

C

T

L

L

G

G

V

I

L
x
I

T

T

E

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

K

K

P

P

R

R

binding flavin-adenine dinucleotide: Q76 (= Q27), G77 (= G28), G78 (= G29), N79 (= N30), T80 (≠ S31), G81 (≠ K32), M82 (≠ A33), G85 (= G36), S86 (≠ R37), L139 (≠ P88), G140 (≠ P89), A141 (= A90), C145 (≠ A95), G149 (= G99), N150 (≠ M100), A152 (= A102), T153 (= T103), G157 (= G107), G207 (= G157), I212 (≠ L162)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 333, 337, 348, 379, 381, 388, 423
binding flavin-adenine dinucleotide: 39, 75, 422, 459
binding zinc ion: 381, 388, 422

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
31% identity, 42% coverage: 27:174/349 of query aligns to 76:224/466 of 6lpuA

query
sites
6lpuA

Q

Q

G
|
G

N
|
N

S
x
T

K
x
G

A

M

F

V

L

G

G
|
G

R
x
S

E

V

V

P

A

V

G

F

E

D

-

E

-

I

V

I

L

L

D

S

T

T

R

A

A

R

H

M

R

N

G

R

I

V

V

L

R

S

Y

F

D

H

P

S

T

V

E

S

L

G

V

I

V

L

T

V

V

C

R

Q

A

A

G

G

T

C

P

V

L

L

S

E

E

E

L

L

L

S

A

R

A

Y

L

V

D

E

A

E

A

R

G

D

Q

F

M

I

L

M

P

P

C

L

E

D

P
x
L

P
x
G

A
|
A

F

K

G

G

E

S

G

-

A
x
C

T
x
H

V

I

G
|
G

M
x
N

I

V

A
|
A

T
|
T

G

N

L
x
A

S

G

G

G

P

L

R

R

R

F

P

L

W

R

S

Y

G

G

S

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

T

L

R

E

V

V

I

V

T

L

G

A

L

D

G

G

Q

T

H

V

L

L

R

D

F

C

G

L

G

T

E

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

L

L

S

K

R

Q

L

L

M

F

A

I

G

G

S

S

Y
x
E

G
|
G

C

T

L

L

G

G

V
x
I

L
x
I

T

T

E

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

K

K

P

P

R

R

binding flavin-adenine dinucleotide: G77 (= G28), G78 (= G29), N79 (= N30), T80 (≠ S31), G81 (≠ K32), G85 (= G36), S86 (≠ R37), L139 (≠ P88), G140 (≠ P89), A141 (= A90), C145 (≠ A95), H146 (≠ T96), G148 (= G98), G149 (= G99), N150 (≠ M100), A152 (= A102), T153 (= T103), A155 (≠ L105), E206 (≠ Y156), G207 (= G157), I211 (≠ V161), I212 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39, 75, 422, 459
binding (2s)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 423
binding zinc ion: 381, 388, 422

6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
31% identity, 42% coverage: 27:174/349 of query aligns to 76:224/466 of 6lpqA

query
sites
6lpqA

Q

Q

G
|
G

N
|
N

S
x
T

K
x
G

A
x
M

F

V

L

G

G
|
G

R
x
S

E

V

V

P

A

V

G

F

E

D

-

E

-

I

V

I

L

L

D

S

T

T

R

A

A

R

H

M

R

N

G

R

I

V

V

L

R

S

Y

F

D

H

P

S

T

V

E

S

L

G

V

I

V

L

T

V

V

C

R

Q

A

A

G

G

T

C

P

V

L

L

S

E

E

E

L

L

L

S

A

R

A

Y

L

V

D

E

A

E

A

R

G

D

Q

F

M

I

L

M

P

P

C

L

E

D

P
x
L

P
x
G

A
|
A

F

K

G

G

E

S

G

-

A
x
C

T
x
H

V

I

G

G

G
|
G

M
x
N

I

V

A
|
A

T
|
T

G

N

L
x
A

S

G

G
|
G

P

L

R

R

R

F

P

L

W

R

S

Y

G

G

S

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

T

L

R

E

V

V

I

V

T

L

G

A

L

D

G

G

Q

T

H

V

L

L

R

D

F

C

G

L

G

T

E

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

L

L

S

K

R

Q

L

L

M

F

A

I

G

G

S

S

Y
x
E

G
|
G

C

T

L

L

G

G

V
x
I

L
x
I

T

T

E

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

K

K

P

P

R

R

binding flavin-adenine dinucleotide: G77 (= G28), G78 (= G29), N79 (= N30), T80 (≠ S31), G81 (≠ K32), G85 (= G36), S86 (≠ R37), L139 (≠ P88), G140 (≠ P89), A141 (= A90), C145 (≠ A95), H146 (≠ T96), G149 (= G99), N150 (≠ M100), A152 (= A102), T153 (= T103), A155 (≠ L105), G157 (= G107), E206 (≠ Y156), G207 (= G157), I211 (≠ V161), I212 (≠ L162)
binding d-malate: M82 (≠ A33)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39, 75, 422, 459
binding d-malate: 333, 337, 348, 379, 381, 388, 422, 423
binding zinc ion: 381, 388, 422

6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
31% identity, 42% coverage: 27:174/349 of query aligns to 76:224/466 of 6lppA

query
sites
6lppA

Q

Q

G
|
G

N
|
N

S
x
T

K
x
G

A
x
M

F

V

L

G

G
|
G

R
x
S

E

V

V

P

A

V

G

F

E

D

-

E

-

I

V

I

L

L

D

S

T

T

R

A

A

R

H

M

R

N

G

R

I

V

V

L

R

S

Y

F

D

H

P

S

T

V

E

S

L

G

V

I

V

L

T

V

V

C

R

Q

A

A

G

G

T

C

P

V

L

L

S

E

E

E

L

L

L

S

A

R

A

Y

L

V

D

E

A

E

A

R

G

D

Q

F

M

I

L

M

P

P

C

L

E

D

P
x
L

P
x
G

A
|
A

F

K

G

G

E

S

G

-

A
x
C

T

H

V

I

G

G

G
|
G

M
x
N

I

V

A
|
A

T
|
T

G

N

L
x
A

S

G

G
|
G

P

L

R

R

R

F

P

L

W

R

S

Y

G

G

S

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

T

L

R

E

V

V

I

V

T

L

G

A

L

D

G

G

Q

T

H

V

L

L

R

D

F

C

G

L

G

T

E

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

L

L

S

K

R

Q

L

L

M

F

A

I

G

G

S

S

Y

E

G
|
G

C

T

L

L

G

G

V

I

L
x
I

T

T

E

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

K

K

P

P

R

R

binding flavin-adenine dinucleotide: G77 (= G28), G78 (= G29), N79 (= N30), T80 (≠ S31), G81 (≠ K32), M82 (≠ A33), G85 (= G36), S86 (≠ R37), L139 (≠ P88), G140 (≠ P89), A141 (= A90), C145 (≠ A95), G149 (= G99), N150 (≠ M100), A152 (= A102), T153 (= T103), A155 (≠ L105), G157 (= G107), G207 (= G157), I212 (≠ L162)

Sites not aligning to the query:

binding (2R)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 390, 423
binding flavin-adenine dinucleotide: 39, 75, 422, 423
binding zinc ion: 381, 388, 422

6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
31% identity, 42% coverage: 27:174/349 of query aligns to 77:225/467 of 6lpnB

query
sites
6lpnB

Q

Q

G
|
G

N
|
N

S
x
T

K
x
G

A
x
M

F

V

L

G

G
|
G

R
x
S

E

V

V

P

A

V

G

F

E

D

-

E

-

I

V

I

L

L

D

S

T

T

R

A

A

R

H

M

R

N

G

R

I

V

V

L

R

S

Y

F

D

H

P

S

T

V

E

S

L

G

V

I

V

L

T

V

V

C

R

Q

A

A

G

G

T

C

P

V

L

L

S

E

E

E

L

L

L

S

A

R

A

Y

L

V

D

E

A

E

A

R

G

D

Q

F

M

I

L

M

P

P

C

L

E

D

P
x
L

P

G

A
|
A

F

K

G

G

E

S

G

-

A
x
C

T
x
H

V

I

G

G

G
|
G

M
x
N

I

V

A
|
A

T
|
T

G

N

L

A

S

G

G

G

P

L

R

R

R

F

P

L

W

R

S

Y

G

G

S

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

T

L

R

E

V

V

I

V

T

L

G

A

L

D

G

G

Q

T

H

V

L

L

R

D

F

C

G

L

G

T

E

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

L

L

S

K

R

Q

L

L

M

F

A

I

G

G

S

S

Y

E

G
|
G

C

T

L

L

G

G

V
x
I

L
x
I

T

T

E

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

K

K

P

P

R

R

binding flavin-adenine dinucleotide: G78 (= G28), G79 (= G29), N80 (= N30), T81 (≠ S31), G82 (≠ K32), M83 (≠ A33), G86 (= G36), S87 (≠ R37), L140 (≠ P88), A142 (= A90), C146 (≠ A95), H147 (≠ T96), G150 (= G99), N151 (≠ M100), A153 (= A102), T154 (= T103), G208 (= G157), I212 (≠ V161), I213 (≠ L162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 40, 76, 423, 460

Query Sequence

>WP_100848112.1 NCBI__GCF_003031005.1:WP_100848112.1
MADFDASSALLDQVNQARANATPLKIQGGNSKAFLGREVAGEVLDTRAHRGIVRYDPTEL
VVTVRAGTPLSELLAALDAAGQMLPCEPPAFGEGATVGGMIATGLSGPRRPWSGSVRDFV
LGTRVITGLGQHLRFGGEVMKNVAGYDLSRLMAGSYGCLGVLTEVSLKVLPKPRQCLSIR
LDIDCTRALANLAEWGQQPLPISAACHDGASLYLRLEGGEGSVTAAHQRLGGEPLDSVFW
RDLNEQRLPFFDEGLPLWRLSLPNNLGPLDLPGAQLIDWAGAQRWLKSDKPHIQILAQEL
GGHATCFTHGASDSPFQPLAWTLLRYHRQLKAQLDPQGLFNPGRMYAEF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory