SitesBLAST
Comparing WP_101289457.1 NCBI__GCF_002844595.1:WP_101289457.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
55% identity, 98% coverage: 6:472/475 of query aligns to 3:465/465 of 3pm9A
- active site: A149 (= A154), L159 (≠ N164)
- binding flavin-adenine dinucleotide: P69 (= P72), Q70 (= Q73), G71 (= G74), G72 (= G75), N73 (= N76), T74 (= T77), G75 (= G78), L76 (= L79), G79 (= G82), Q80 (= Q83), L91 (= L96), L133 (= L138), G134 (≠ A139), A135 (≠ S140), C139 (= C144), T140 (≠ Q145), G142 (= G147), G143 (= G148), S146 (≠ A151), T147 (= T152), A149 (= A154), G150 (= G155), E200 (= E205), G201 (= G206), I205 (= I210), I206 (= I211), E423 (= E430)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
40% identity, 92% coverage: 34:472/475 of query aligns to 37:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R334), T337 (≠ S338), K348 (= K349), Y379 (≠ F382), H381 (= H384), H388 (= H391), H423 (= H431)
- binding flavin-adenine dinucleotide: W39 (= W36), P75 (= P72), Q76 (= Q73), G77 (= G74), G78 (= G75), N79 (= N76), T80 (= T77), G81 (= G78), M82 (≠ L79), G85 (= G82), S86 (≠ Q83), L139 (= L138), G140 (≠ A139), A141 (≠ S140), C145 (= C144), G149 (= G148), N150 (= N149), A152 (= A151), T153 (= T152), G157 (= G156), G207 (= G206), I212 (= I211), E422 (= E430), N459 (= N467)
- binding zinc ion: H381 (= H384), H388 (= H391), E422 (= E430)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
40% identity, 92% coverage: 34:472/475 of query aligns to 37:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W36), P75 (= P72), G77 (= G74), G78 (= G75), N79 (= N76), T80 (= T77), G81 (= G78), G85 (= G82), S86 (≠ Q83), L139 (= L138), G140 (≠ A139), A141 (≠ S140), C145 (= C144), H146 (≠ Q145), G148 (= G147), G149 (= G148), N150 (= N149), A152 (= A151), T153 (= T152), A155 (= A154), E206 (= E205), G207 (= G206), I211 (= I210), I212 (= I211), E422 (= E430), N459 (= N467)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R334), T337 (≠ S338), K348 (= K349), Y379 (≠ F382), H381 (= H384), H388 (= H391), H423 (= H431)
- binding zinc ion: H381 (= H384), H388 (= H391), E422 (= E430)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
40% identity, 92% coverage: 34:472/475 of query aligns to 37:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W36), P75 (= P72), G77 (= G74), G78 (= G75), N79 (= N76), T80 (= T77), G81 (= G78), G85 (= G82), S86 (≠ Q83), L139 (= L138), G140 (≠ A139), A141 (≠ S140), C145 (= C144), H146 (≠ Q145), G149 (= G148), N150 (= N149), A152 (= A151), T153 (= T152), A155 (= A154), G157 (= G156), E206 (= E205), G207 (= G206), I211 (= I210), I212 (= I211), E422 (= E430), N459 (= N467)
- binding d-malate: M82 (≠ L79), R333 (= R334), T337 (≠ S338), K348 (= K349), Y379 (≠ F382), H381 (= H384), H388 (= H391), E422 (= E430), H423 (= H431)
- binding zinc ion: H381 (= H384), H388 (= H391), E422 (= E430)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
40% identity, 92% coverage: 34:472/475 of query aligns to 37:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R334), T337 (≠ S338), K348 (= K349), Y379 (≠ F382), H381 (= H384), H388 (= H391), N390 (= N393), H423 (= H431)
- binding flavin-adenine dinucleotide: W39 (= W36), P75 (= P72), G77 (= G74), G78 (= G75), N79 (= N76), T80 (= T77), G81 (= G78), M82 (≠ L79), G85 (= G82), S86 (≠ Q83), L139 (= L138), G140 (≠ A139), A141 (≠ S140), C145 (= C144), G149 (= G148), N150 (= N149), A152 (= A151), T153 (= T152), A155 (= A154), G157 (= G156), G207 (= G206), I212 (= I211), E422 (= E430), H423 (= H431)
- binding zinc ion: H381 (= H384), H388 (= H391), E422 (= E430)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
40% identity, 92% coverage: 34:472/475 of query aligns to 38:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W36), P76 (= P72), G78 (= G74), G79 (= G75), N80 (= N76), T81 (= T77), G82 (= G78), M83 (≠ L79), G86 (= G82), S87 (≠ Q83), L140 (= L138), A142 (≠ S140), C146 (= C144), H147 (≠ Q145), G150 (= G148), N151 (= N149), A153 (= A151), T154 (= T152), G208 (= G206), I212 (= I210), I213 (= I211), E423 (= E430), N460 (= N467)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
40% identity, 92% coverage: 34:472/475 of query aligns to 90:517/521 of Q8N465
- S109 (≠ T53) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V71) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G75) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V93) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L99) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ M118) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P135) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A151) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (= A177) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G179) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S323) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R334) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ S338) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ S347) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K349) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ M367) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ C377) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H384) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G386) to V: slight reduction in catalytic activity
- N439 (= N389) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H391) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N393) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (= V394) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ Q396) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E430) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H431) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G432) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 98% coverage: 7:472/475 of query aligns to 29:496/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
29% identity, 90% coverage: 46:473/475 of query aligns to 47:465/467 of 7qh2C