SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_101442924.1 NCBI__GCF_002846395.1:WP_101442924.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 85% coverage: 22:245/263 of query aligns to 24:252/265 of P07821

query
sites
P07821

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K50 (= K48) mutation to Q: Lack of activity.
D172 (= D167) mutation to E: Lack of activity.
E173 (= E168) mutation to A: Lack of activity.

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
30% identity, 89% coverage: 9:241/263 of query aligns to 2:236/240 of 6mjpA

query
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6mjpA

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binding calcium ion: E111 (≠ R114), D115 (= D121)

3c4jA Abc protein artp in complex with atp-gamma-s
31% identity, 91% coverage: 9:248/263 of query aligns to 3:241/242 of 3c4jA

query
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3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y19), V18 (= V24), S38 (≠ N44), G39 (= G45), S40 (≠ A46), G41 (= G47), K42 (= K48), S43 (= S49), T44 (= T50)

3c41J Abc protein artp in complex with amp-pnp/mg2+
31% identity, 91% coverage: 9:248/263 of query aligns to 3:241/242 of 3c41J

query
sites
3c41J

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binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y19), V18 (= V24), S38 (≠ N44), G39 (= G45), G41 (= G47), K42 (= K48), S43 (= S49), T44 (= T50)
binding magnesium ion: S43 (= S49), D163 (= D167)

2olkA Abc protein artp in complex with adp-beta-s
31% identity, 91% coverage: 9:248/263 of query aligns to 3:241/242 of 2olkA

query
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2olkA

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y19), V18 (= V24), S38 (≠ N44), G39 (= G45), S40 (≠ A46), G41 (= G47), K42 (= K48), S43 (= S49), T44 (= T50)

2oljA Abc protein artp in complex with adp/mg2+
31% identity, 91% coverage: 9:248/263 of query aligns to 3:241/242 of 2oljA

query
sites
2oljA

V

M

V

I

E

D

V

V

H

H

D

Q

L

L

T

K

V

K

S

S

Y
x
F

E

G

R

S

K

L

P

E

V
|
V

L

L

W

K

G

G

I

I

D

N

L

V

T

H

L

I

P

R

A

E

G

G

A

E

L

V

V

V

G

V

V

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

A

C

M

L

M

N

D

L

L

L

L

E

P

D

V

F

S

D

S

E

G

G

Y

E

V

I

R

I

I

I

-

D

-

G

-

I

-

N

-

L

-

K

-

A

F

K

D

D

Q

T

P

N

L

L

K

N

Q

K

V

V

Y

R

S

E

R

E

I

V

S

G

Y

M

V

V

P

F

Q

Q

R

R

E

F

S

N

V

L

D

-

W

-

D

-

F

F

P

P

A

H

-

M

S

T

V

V

F

L

D

N

V

N

V

I

L

T

M

L

G

A

R

-

Y

-

G

-

Q

P

L

M

G

K

L

V

F

R

K

K

R

W

P

P

R

R

K

E

A

K

D

A

K

E

D

A

A

K

A

A

M

M

E

E

A

L

L

L

E

D

K

K

V

V

G

G

M

L

Q

K

S

D

F

K

A

A

N

H

R

A

Q

Y

I

P

S

D

Q

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

V

V

F

A

L

I

A

A

R

R

A

A

L

L

A

A

Q

M

N

E

A

P

D

K

V

I

Y

M

F

L

M

F

D

D

E

E

P

P

F

T

A

S

G

A

V

L

D

D

I

P

A

E

T

M

E

V

T

G

A

E

I

V

I

L

Q

S

L

V

L

M

R

K

Q

Q

M

L

R

A

D

N

Q

E

G

G

K

M

T

T

V

M

I

V

V

V

H

T

H

H

D

E

L

M

Q

G

S

F

A

A

S

R

E

E

Y

V

F

G

D

D

W

R

V

V

V

L

L

F

L

M

N

D

-

G

M

G

R

Y

L

I

V

I

A

E

S

E

G

G

P

K

T

P

E

E

Q

D

V

L

L

F

N

D

Q

R

E

P

L

Q

L

H

E

E

Q

R

T

T

Y

K

G

-

K

-

L

-

T

A

L

F

L

L

S

S

K

K

V

V

binding adenosine-5'-diphosphate: F13 (≠ Y19), V18 (= V24), S38 (≠ N44), G39 (= G45), S40 (≠ A46), G41 (= G47), K42 (= K48), S43 (= S49), T44 (= T50)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
31% identity, 87% coverage: 8:237/263 of query aligns to 1:232/241 of 4u00A

query
sites
4u00A

P

P

V

I

E

R

V

I

H

R

D

N

L

L

T

H

V

K

S

W

Y
x
F

E

G

R

P

K

L

P

H

V
|
V

L

L

W

K

G

G

I

I

D

H

L

L

T

E

L

V

P

A

A

P

G

G

A

E

L

K

V

L

G

V

V

I

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

A

T

M

I

M

N

D

R

L

L

L

E

P

D

V

F

S

Q

S

E

G

G

Y

E

V

V

R

V

I

V

F

D

-

G

-

L

-

S

-

V

-

K

-

D

D

D

Q

R

P

A

L

L

K

R

Q

E

V

I

Y

R

S

R

R

E

I

V

S

G

Y

M

V

V

P

F

Q

Q

R

Q

E

F

S

N

V

L

D

-

W

-

D

-

F

F

P

P

A

H

-

M

S

T

V

V

F

L

D

E

V

N

V

V

L

T

M

L

G

A

R

-

Y

-

G

-

Q

P

L

M

G

R

L

V

F

R

K

R

R

W

P

P

R

R

K

E

A

K

D

A

K

E

D

K

A

K

A

A

M

L

E

E

A

L

L

L

E

E

K

R

V

V

G

G

M

I

Q

L

S

D

F

Q

A

A

N

R

R

K

Q

Y

I

P

S

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

V

V

F

A

L

I

A

A

R

R

A

A

L

L

A

A

Q

M

N

E

A

P

D

K

V

I

Y

M

F

L

M

F

D

D

E

E

P

P

F

T

A

S

G

A

V

L

D

D

I

P

A

E

T

M

E

V

T

G

A

E

I

V

I

L

Q

D

L

V

L

M

R

R

Q

D

M

L

R

A

D

Q

Q

G

G

G

K

M

T

T

V

M

I

V

V

V

H

T

H

H

D

E

L

M

Q

G

S

F

A

A

S

R

E

E

Y

V

F

A

D

D

W

R

V

V

L

F

L

M

N

D

-

G

M

G

R

Q

L

I

V

V

A

E

S

E

G

G

P

R

T

P

E

E

Q

E

V

I

L

F

N

T

Q

R

E

P

L

K

L

E

E

E

Q

R

T

T

binding adenosine-5'-diphosphate: F12 (≠ Y19), V17 (= V24), S37 (≠ N44), G38 (= G45), S39 (≠ A46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
30% identity, 87% coverage: 14:241/263 of query aligns to 7:236/238 of 6s8nB

query
sites
6s8nB

D

N

L

L

T

A

V

K

S

A

Y

Y

E

K

R

G

K

R

P

R

V

V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L

L

K

H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P

P

Q

Q

R

E

E

A

S

S

V

I

D

F

W
x
R

D

R

F

L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G

V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q
x
M

I
x
G

S
x
Q

Q

S

L

L

S

S

G

G

Q

E

Q

R

R

R

V

V

F

E

L

I

A

A

R

R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E

E

P

P

F

F

A

A

G

G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H

H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

G

L

G

G

K

E

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ W91), M133 (≠ Q139), G134 (≠ I140), Q135 (≠ S141)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
30% identity, 87% coverage: 14:241/263 of query aligns to 7:236/238 of 6s8gA

query
sites
6s8gA

D

N

L

L

T

A

V

K

S

A

Y
|
Y

E

K

R

G

K
x
R

P

R

V

V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L

L

K

H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P

P

Q
|
Q

R

E

E

A

S

S

V

I

D

F

W

R

D

R

F

L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G

V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q

M

I

G

S

Q

Q
x
S

L

L

S
|
S

G

G

Q
x
E

Q

R

R

R

V

V

F

E

L

I

A

A

R

R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E

E

P

P

F

F

A

A

G

G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H

H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

G

L

G

G

K

E

binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y19), R15 (≠ K22), N37 (= N44), G40 (= G47), K41 (= K48), T42 (≠ S49), T43 (= T50), Q84 (= Q85), S136 (≠ Q142), S138 (= S144), E141 (≠ Q147)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
30% identity, 86% coverage: 14:240/263 of query aligns to 7:235/235 of 6mhzA

query
sites
6mhzA

D

N

L

L

T

A

V

K

S

A

Y
|
Y

E

K

R

G

K

R

P

R

V

V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L

L

K

H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P

P

Q
|
Q

R

E

E

A

S

S

V

I

D

F

W

R

D

R

F

L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G

V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q

M

I

G

S

Q

Q
x
S

L

L

S
|
S

G
|
G

Q

E

Q

R

R

R

V

V

F

E

L

I

A

A

R

R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E
|
E

P

P

F

F

A

A

G
|
G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H
|
H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

G

L

G

G

binding adp orthovanadate: Y12 (= Y19), N37 (= N44), G38 (= G45), G40 (= G47), K41 (= K48), T42 (≠ S49), T43 (= T50), Q84 (= Q85), S136 (≠ Q142), S138 (= S144), G139 (= G145), G140 (= G146), E162 (= E168), G166 (= G172), H194 (= H200)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
30% identity, 86% coverage: 14:238/263 of query aligns to 7:233/234 of 6b89A

query
sites
6b89A

D

N

L

L

T

A

V

K

S

A

Y
|
Y

E

K

R

G

K
x
R

P

R

V
|
V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L
|
L

K
x
H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P
|
P

Q
|
Q

R

E

E
x
A

S
|
S

V

I

D
x
F

W
x
R

D
x
R

F
x
L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G
x
V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q

M

I

G

S
x
Q

Q

S

L

L

S

S

G

G

Q

E

Q

R

R

R

V

V

F

E

L

I

A

A

R
|
R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E

E

P

P

F

F

A

A

G

G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H

H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y19), R15 (≠ K22), V17 (= V24), N37 (= N44), G38 (= G45), G40 (= G47), K41 (= K48), T42 (≠ S49), T43 (= T50)
binding magnesium ion: T42 (≠ S49), Q84 (= Q85)
binding novobiocin: L71 (= L73), H72 (≠ K74), P83 (= P84), A86 (≠ E87), S87 (= S88), F89 (≠ D90), R90 (≠ W91), R91 (≠ D92), L92 (≠ F93), V101 (≠ G104), Q135 (≠ S141), R149 (= R155)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
30% identity, 86% coverage: 14:238/263 of query aligns to 7:233/234 of 4p31A

query
sites
4p31A

D

N

L

L

T

A

V

K

S

A

Y
|
Y

E

K

R

G

K
x
R

P

R

V
|
V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L

L

K

H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P

P

Q
|
Q

R

E

E

A

S

S

V

I

D

F

W

R

D

R

F

L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G

V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q

M

I

G

S

Q

Q

S

L

L

S

S

G

G

Q

E

Q

R

R

R

V

V

F

E

L

I

A

A

R

R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E

E

P

P

F

F

A

A

G

G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H

H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y19), R15 (≠ K22), V17 (= V24), G38 (= G45), G40 (= G47), K41 (= K48), T42 (≠ S49), T43 (= T50)
binding magnesium ion: T42 (≠ S49), Q84 (= Q85)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
30% identity, 86% coverage: 14:238/263 of query aligns to 7:233/233 of 6b8bA

query
sites
6b8bA

D

N

L

L

T

A

V

K

S

A

Y
|
Y

E

K

R

G

K
x
R

P

R

V
|
V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L

L

K

H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P

P

Q

Q

R

E

E

A

S

S

V

I

D
x
F

W
x
R

D
x
R

F

L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G

V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q

M

I

G

S

Q

Q

S

L

L

S

S

G

G

Q

E

Q

R

R

R

V

V

F

E

L

I

A

A

R

R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E

E

P

P

F

F

A

A

G

G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H

H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y19), R15 (≠ K22), V17 (= V24), G38 (= G45), G40 (= G47), K41 (= K48), T42 (≠ S49), T43 (= T50)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ D90), R90 (≠ W91), R91 (≠ D92)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 76% coverage: 7:207/263 of query aligns to 2:209/648 of P75831

query
sites
P75831

N

T

P

P

V

L

E

E

V

L

H

K

D

D

L

I

T

R

V

R

S

S

Y

Y

-

P

-

A

-

G

-

D

E

E

R

Q

K

V

P

E

V

V

L

L

W

K

G

G

I

I

D

S

L

L

T

D

L

I

P

Y

A

A

G

G

A

E

L

M

V

V

G

A

V

I

I

V

G

G

P

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

I

M

K

N

A

I

M

L

M

G

D

C

L

L

L

D

P

K

V

A

S

T

S

S

G

G

Y

T

V

Y

R

R

I

V

F

A

D

G

Q

Q

P

D

L

V

K

A

Q

T

V

L

Y

D

S

A

R

D

I

A

S

L

Y

A

V

Q

P

L

Q

R

R

R

E

E

S

H

V

F

D

G

W

F

D

I

F

F

P

Q

-

R

-

Y

-

H

-

L

-

S

-

H

-

L

A

T

S

A

V

E

F

Q

D

N

V

V

V

E

L

V

M

P

G

A

R

V

Y

Y

G

A

Q

G

L

L

G

-

L

-

F

-

K

-

R

-

P

E

R

R

K

K

A

Q

D

R

K

L

D

L

A

R

A

A

M

Q

E

E

A

L

L

L

E

Q

K

R

V

L

G

G

M

L

Q

E

S

D

F

R

A

T

N

E

R

Y

Q

Y

I

P

S

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

V

V

F

S

L

I

A

A

R

R

A

A

L

L

A

M

Q

N

N

G

A

G

D

Q

V

V

Y

I

F

L

M

A

D
|
D

E

E

P

P

F

T

A

G

G

A

V

L

D

D

I

S

A

H

T

S

E

G

T

E

A

E

I

V

I

M

Q

A

L

I

L

L

R

H

Q

Q

M

L

R

R

D

D

Q

R

G

G

K

H

T

T

V

V

I

I

V

I

V

V

H

T

H

H

D

D

L

P

Q

Q

S

V

A

A

S

A

E

Q

K47 (= K48) mutation to L: Lack of activity.
D169 (= D167) mutation to N: Lack of activity.

8xokA Cryo-em structure of human abcc4 (see paper)
31% identity, 85% coverage: 9:232/263 of query aligns to 404:619/1206 of 8xokA

query
sites
8xokA

V

M

V

V

E

H

V

V

H

Q

D

D

L

F

T

T

V

A

S

F

Y

W

E

D

R

K

-

A

-

S

-

E

K

T

P

P

V

T

L

L

W

Q

G

G

I

L

D

S

L

F

T

T

L

V

P

R

A

P

G

G

A

E

L

L

V

L

G

A

V

V

I

V

G

G

P

P

N

V

G

G

A

A

G

G

K

K

S

S

T

S

L

L

I

L

K

S

A

A

M

V

M

L

D

G

L

E

L

L

P

A

V

P

S

S

S

H

G

G

Y

L

V

V

R

-

I

-

F

-

D

-

Q

-

P

-

L

-

K

-

Q

S

V

V

Y

H

S

G

R

R

I

I

S

A

Y

Y

V

V

P

S

Q

Q

R

Q

E

P

S

-

V

-

D

-

W

W

D

V

F

F

P

S

A

G

S

T

V

L

F

R

D

S

V

N

V

I

L

L

M

F

G

G

R

K

Y

K

G

Y

Q

E

L

K

G

E

L

R

F

Y

K

E

R

K

P

V

R

I

K

K

A

A

-

C

-

A

-

L

D

K

K

K

D

D

A

-

M

L

E

Q

A

L

L

L

E

E

K

D

V

G

G

D

M

L

Q

T

S

V

F

I

A

G

N

D

R

R

Q

G

I

-

S

T

Q

T

L

L

S

S

G

G

Q

Q

Q

K

Q

A

R

R

V

V

F

N

L

L

A

A

R

R

A

A

L

V

A

Y

Q

Q

N

D

A

A

D

D

V

I

Y

Y

F

L

M

L

D

D

E

D

P

P

F

L

A

S

G

A

V

V

D

D

I

A

A

E

T

V

E

S

T

R

A

H

I

L

I

F

Q

E

L

L

L

C

R

I

Q

C

M

Q

R

I

D

L

Q

H

G

E

K

K

T

I

V

T

I

I

V

L

V

V

H

T

H

H

D

Q

L

L

Q

Q

S

Y

A

L

S

K

E

A

Y

A

F

S

D

Q

W

I

V

L

V

I

L

L

L

K

N

D

M

G

R

K

L

M

V

V

A

Q

S

K

G

G

P

T

T

Y

E

T

Q

E

V

F

L

L

N

K

Q

S

E

E

Sites not aligning to the query:

binding 2-[2-[(1~{S},2~{S},4~{S},5'~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{R},16~{S})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol: 107, 128, 131, 135, 210, 221, 222, 368, 372, 652, 655, 656, 659, 688, 689, 696, 697, 777, 781, 785, 785, 788, 789, 791, 888, 904

6mbnA Lptb e163q in complex with atp (see paper)
29% identity, 87% coverage: 14:241/263 of query aligns to 8:237/241 of 6mbnA

query
sites
6mbnA

D

N

L

L

T

A

V

K

S

A

Y
|
Y

E

K

R

G

K
x
R

P

R

V
|
V

L

V

W

E

G

D

I

V

D

S

L

L

T

T

L

V

P

N

A

S

G

G

A

E

L

I

V

V

G

G

V

L

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

A

M

M

V

D

G

L

I

L

V

P

P

V

R

S

D

S

A

G

G

Y

N

V

I

R

I

I

I

F

D

D

D

Q

D

-

D

-

I

-

S

-

L

P

P

L

L

K

H

Q

A

V

R

Y

A

S

R

R

R

-

G

I

I

S

G

Y

Y

V

L

P

P

Q

Q

R

E

E

A

S

S

V

I

D

F

W

R

D

R

F

L

P

-

A

-

S

S

V

V

F

Y

D

D

V

N

V

L

L

M

M

A

G

V

R

L

Y

Q

G

I

Q

R

L

D

G

D

L

L

F

S

K

A

R

E

P

Q

R

R

K

E

A

-

D

-

K

-

D

D

A

R

A

A

M

N

E

E

A

L

L

M

E

E

K

E

V

F

G

H

M

I

Q

E

S

H

F

L

A

R

N

D

R

S

Q

M

I

G

S

Q

Q

S

L

L

S

S

G

G

Q

E

Q

R

R

R

V

V

F

E

L

I

A

A

R

R

A

A

L

L

A

A

Q

A

N

N

A

P

D

K

V

F

Y

I

F

L

M

L

D

D

E

Q

P

P

F

F

A

A

G

G

V

V

D

D

I

P

A

I

T

S

E

V

T

I

A

D

I

I

I

K

Q

R

L

I

R

E

Q

H

M

L

R

R

D

D

Q

S

G

G

K

L

T

G

V

V

I

L

V

I

V

T

H

D

H
|
H

D

N

L

V

Q

R

S

E

A

T

S

L

E

A

Y

V

F

C

D

E

W

R

V

A

V

Y

L

I

L

V

N

S

M

Q

-

G

R

H

L

L

V

I

A

A

S

H

G

G

P

T

T

P

E

T

Q

E

V

I

L

L

N

Q

Q

D

E

E

L

H

L

V

E

K

Q

R

T

V

Y

Y

G

L

G

G

K

E

binding adenosine-5'-triphosphate: Y13 (= Y19), R16 (≠ K22), V18 (= V24), N38 (= N44), G39 (= G45), G41 (= G47), K42 (= K48), T43 (≠ S49), T44 (= T50), H195 (= H200)

8iz9A Cryo-em structure of pge1-bound hmrp4
30% identity, 92% coverage: 1:242/263 of query aligns to 384:622/1224 of 8iz9A

query
sites
8iz9A

M

L

I

L

L

L

Q

D

V

E

E

I

N

S

P

K

V

M

V

V

E

H

V

V

H

Q

D

D

L

F

T

T

V

A

S

F

Y

W

E

D

R

K

-

A

-

S

-

E

K

T

P

P

V

T

L

L

W

Q

G

G

I

L

D

S

L

F

T

T

L

V

P

R

A

P

G

G

A

E

L

L

V

L

G

A

V

V

I

V

G

G

P

P

N

V

G

G

A

A

G

G

K

K

S

S

T

S

L

L

I

L

K

S

A

A

M

V

M

L

D

G

L

E

L

L

P

A

V

P

S

S

S

H

G

G

Y

L

V

V

R

-

I

-

F

-

D

-

Q

-

P

-

L

-

K

-

Q

S

V

V

Y

H

S

G

R

R

I

I

S

A

Y

Y

V

V

P

S

Q

Q

R

Q

E

P

S

-

V

-

D

-

W

W

D

V

F

F

P

S

A

G

S

T

V

L

F

R

D

S

V

N

V

I

L

L

M

F

G

G

R

K

Y

K

G

Y

Q

E

L

K

G

E

L

R

F

Y

K

E

R

K

P

V

R

I

K

K

A

A

-

C

-

A

-

L

D

K

K

K

D

D

A

-

M

L

E

Q

A

L

L

L

E

E

K

D

V

G

G

D

M

L

Q

T

S

V

F

I

A

G

N

D

R

R

Q

G

I

-

S

T

Q

T

L

L

S

S

G

G

Q

Q

Q

K

Q

A

R

R

V

V

F

N

L

L

A

A

R

R

A

A

L

V

A

Y

Q

Q

N

D

A

A

D

D

V

I

Y

Y

F

L

M

L

D

D

E

D

P

P

F

L

A

S

G

A

V

V

D

D

I

A

A

E

T

V

E

S

T

R

A

H

I

L

I

F

Q

E

L

L

L

C

R

I

Q

C

M

Q

R

I

D

L

Q

H

G

E

K

K

T

I

V

T

I

I

V

L

V

V

H

T

H

H

D

Q

L

L

Q

Q

S

Y

A

L

S

K

E

A

Y

A

F

S

D

Q

W

I

V

L

V

I

L

L

L

K

N

D

M

G

R

K

L

M

V

V

A

Q

S

K

G

G

P

T

T

Y

E

T

Q

E

V

F

L

L

N

K

Q

S

-

G

-

I

-

D

-

F

-

G

E

S

L

L

E

E

Q

N

T

R

Y

S

G

E

G

G

K

K

L

V

Sites not aligning to the query:

binding 7-[(1R,3R)-3-hydroxy-2-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-5-oxocyclopentyl]heptanoic acid: 357, 917, 921

8bwrA Cryo-em structure of nanodisc-reconstituted wildtype human mrp4 (in complex with prostaglandin e2) (see paper)
32% identity, 83% coverage: 7:223/263 of query aligns to 393:601/1195 of 8bwrA

query
sites
8bwrA

N

N

P

K

V

M

V

V

E

H

V

V

H

Q

D

D

L

F

T

T

V

A

S

F

Y

W

E

D

R

K

-

A

-

S

-

E

K

T

P

P

V

T

L

L

W

Q

G

G

I

L

D

S

L

F

T

T

L

V

P

R

A

P

G

G

A

E

L

L

V

L

G

A

V

V

I

V

G

G

P

P

N

V

G

G

A

A

G

G

K

K

S

S

T

S

L

L

I

L

K

S

A

A

M

V

M

L

D

G

L

E

L

L

P

A

V

P

S

S

S

H

G

G

Y

L

V

V

R

-

I

-

F

-

D

-

Q

-

P

-

L

-

K

-

Q

S

V

V

Y

H

S

G

R

R

I

I

S

A

Y

Y

V

V

P

S

Q

Q

R

Q

E

P

S

-

V

-

D

-

W

W

D

V

F

F

P

S

A

G

S

T

V

L

F

R

D

S

V

N

V

I

L

L

M

F

G

G

R

K

Y

K

G

Y

Q

E

L

K

G

E

L

R

F

Y

K

E

R

K

P

V

R

I

K

K

A

A

-

C

-

A

-

L

D

K

K

K

D

D

A

-

M

L

E

Q

A

L

L

L

E

E

K

D

V

G

G

D

M

L

Q

T

S

V

F

I

A

G

N

D

R

R

Q

G

I

-

S

T

Q

T

L

L

S

S

G

G

Q

Q

Q

K

Q

A

R

R

V

V

F

N

L

L

A

A

R

R

A

A

L

V

A

Y

Q

Q

N

D

A

A

D

D

V

I

Y

Y

F

L

M

L

D

D

E

D

P

P

F

L

A

S

G

A

V

V

D

D

I

A

A

E

T

V

E

S

T

R

A

H

I

L

I

F

Q

E

L

L

L

C

R

I

Q

C

M

Q

R

I

D

L

Q

H

G

E

K

K

T

I

V

T

I

I

V

L

V

V

H

T

H

H

D

Q

L

L

Q

Q

S

Y

A

L

S

K

E

A

Y

A

F

S

D

Q

W

I

V

L

V

I

L

L

L

K

N

D

M

G

R

K

L

M

V

V

A

Q

S

K

G

G

Sites not aligning to the query:

binding (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid: 362, 847, 892, 896

8bwqA Cryo-em structure of nanodisc-reconstituted wildtype human mrp4 (in complex with topotecan) (see paper)
32% identity, 83% coverage: 7:223/263 of query aligns to 393:601/1195 of 8bwqA

query
sites
8bwqA

N

N

P

K

V

M

V

V

E

H

V

V

H

Q

D

D

L

F

T

T

V

A

S

F

Y

W

E

D

R

K

-

A

-

S

-

E

K

T

P

P

V

T

L

L

W

Q

G

G

I

L

D

S

L

F

T

T

L

V

P

R

A

P

G

G

A

E

L

L

V

L

G

A

V

V

I

V

G

G

P

P

N

V

G

G

A

A

G

G

K

K

S

S

T

S

L

L

I

L

K

S

A

A

M

V

M

L

D

G

L

E

L

L

P

A

V

P

S

S

S

H

G

G

Y

L

V

V

R

-

I

-

F

-

D

-

Q

-

P

-

L

-

K

-

Q

S

V

V

Y

H

S

G

R

R

I

I

S

A

Y

Y

V

V

P

S

Q

Q

R

Q

E

P

S

-

V

-

D

-

W

W

D

V

F

F

P

S

A

G

S

T

V

L

F

R

D

S

V

N

V

I

L

L

M

F

G

G

R

K

Y

K

G

Y

Q

E

L

K

G

E

L

R

F

Y

K

E

R

K

P

V

R

I

K

K

A

A

-

C

-

A

-

L

D

K

K

K

D

D

A

-

M

L

E

Q

A

L

L

L

E

E

K

D

V

G

G

D

M

L

Q

T

S

V

F

I

A

G

N

D

R

R

Q

G

I

-

S

T

Q

T

L

L

S

S

G

G

Q

Q

Q

K

Q

A

R

R

V

V

F

N

L

L

A

A

R

R

A

A

L

V

A

Y

Q

Q

N

D

A

A

D

D

V

I

Y

Y

F

L

M

L

D

D

E

D

P

P

F

L

A

S

G

A

V

V

D

D

I

A

A

E

T

V

E

S

T

R

A

H

I

L

I

F

Q

E

L

L

L

C

R

I

Q

C

M

Q

R

I

D

L

Q

H

G

E

K

K

T

I

V

T

I

I

V

L

V

V

H

T

H

H

D

Q

L

L

Q

Q

S

Y

A

L

S

K

E

A

Y

A

F

S

D

Q

W

I

V

L

V

I

L

L

L

K

N

D

M

G

R

K

L

M

V

V

A

Q

S

K

G

G

Sites not aligning to the query:

binding (s)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1h-pyrano[3',4':6,7]inolizino[1,2-b]-quinoline-3,14(4h,12h)-dione: 205, 361, 743, 746, 896

8bwpA Cryo-em structure of nanodisc-reconstituted wildtype human mrp4 (in complex with methotrexate) (see paper)
32% identity, 83% coverage: 7:223/263 of query aligns to 393:601/1195 of 8bwpA

query
sites
8bwpA

N

N

P

K

V

M

V

V

E

H

V

V

H

Q

D

D

L

F

T

T

V

A

S

F

Y

W

E

D

R

K

-

A

-

S

-

E

K

T

P

P

V

T

L

L

W

Q

G

G

I

L

D

S

L

F

T

T

L

V

P

R

A

P

G

G

A

E

L

L

V

L

G

A

V

V

I

V

G

G

P

P

N

V

G

G

A

A

G

G

K

K

S

S

T

S

L

L

I

L

K

S

A

A

M

V

M

L

D

G

L

E

L

L

P

A