SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_101444172.1 NCBI__GCF_002846395.1:WP_101444172.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
58% identity, 98% coverage: 6:254/254 of query aligns to 9:257/257 of P0AAH0

query
sites
P0AAH0

S

S

K

K

L

I

E

Q

A

V

I

R

N

N

L

L

D

N

A

F

Y

Y

G

G

D

K

F

F

H

H

A

A

L

L

K

K

G

N

I

I

N

N

I

L

A

D

M

I

E

A

E

K

K

N

A

Q

V

V

T

T

A

A

F

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

T

T

L

F

N

N

R

K

M

M

N

F

D

E

Y

L

I

Y

D

P

G

E

F

Q

R

R

I

A

E

E

G

G

Q

E

V

I

L

L

D

D

G

G

R

D

D

N

I

I

Y

L

A

T

A

N

D

S

V

Q

R

D

V

I

D

A

E

L

L

L

R

R

K

A

E

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

K

P

P

N

T

P

P

F

F

P

P

K

M

S

S

I

I

F

Y

E

D

N

N

V

I

V

A

Y

F

G

G

L

V

K

R

I

L

Q

F

G

E

I

K

K

L

D

S

K

R

N

A

V

D

L

M

Q

D

E

E

A

R

C

V

E

Q

K

W

S

A

L

L

Q

T

H

K

A

A

L

L

W

W

N

N

E

E

V

T

K

K

D

D

K

K

L

L

D

H

K

Q

S

S

A

G

L

Y

A

S

L

L

S

S

G

G

Q

Q

R

R

L

L

C

C

I

I

A

A

R

R

A

G

L

I

A

A

I

I

Q

R

P

P

S

E

V

V

I

L

L

L

M

L

D

D

E

E

P

P

A

C

S

S

A

A

L

L

D

D

P

P

I

I

S

S

T

T

A

G

K

R

I

I

E

E

D

E

L

L

I

I

H

T

E

E

L

L

K

K

K

Q

D

D

Y

Y

T

T

I

V

V

V

I

I

V

V

T

T

H

H

N

N

M

M

Q

Q

A

A

G

A

R

R

V

C

S

S

D

D

H

H

T

T

A

A

F

F

F

M

Y

Y

M

L

G

G

E

E

L

L

V

I

E

E

Y

F

S

S

K

N

T

T

K

D

T

D

M

L

F

F

T

T

S

K

P

P

K

A

D

K

T

K

R

Q

T

T

Q

E

N

D

Y

Y

I

I

T

T

G

G

R

R

F

Y

G

G

G48 (= G45) mutation to I: Loss of phosphate transport.
K49 (= K46) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
39% identity, 94% coverage: 12:249/254 of query aligns to 7:236/241 of 4u00A

query
sites
4u00A

N

N

L

L

D

H

A

K

Y

W

Y
x
F

G

G

D

P

F

L

H

H

A
x
V

L

L

K

K

G

G

I

I

N

H

I

L

A

E

M

V

E

A

E

P

K

G

A

E

V

K

T

L

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

T

L

I

N

N

R

R

M

L

N

E

D

D

Y

F

I

Q

D

-

G

-

F

-

R

-

I

-

E

E

G

G

Q

E

V

V

L

V

D

D

G

G

R

L

D

S

I

V

Y

-

A

K

A

D

D

D

V

R

R

A

V

L

D

R

E

E

L

I

R

R

K

R

E

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

Q

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

E

E

N

N

V

V

V

T

Y

-

G

-

L

-

K

-

I

L

Q

A

G

P

I

M

K

R

D

V

K

R

N

R

V

W

L

P

Q

R

E

E

A

K

C

A

E

E

K

K

S

K

L

A

Q

L

H

E

A

L

L

E

W

R

N

V

E

G

V

I

K

L

D

D

K

Q

L

A

D

R

K

K

S

Y

A

P

L

A

A

Q

L

L

S

S

G

G

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

I

V

E

L

D

D

L

V

I

M

H

R

E

D

L

L

K

A

K

Q

-

G

D

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

E

V

V

S

A

D

D

H

R

T

V

A

V

F

F

F

M

Y

D

M

G

G

G

E

Q

L

I

V

V

E

E

Y

E

S

G

K

R

T

P

K

E

T

E

M

I

F

F

T

T

S

R

P

P

K

K

D

E

T

E

R

R

T

T

Q

R

N

S

Y

F

I

L

binding adenosine-5'-diphosphate: F12 (≠ Y17), V17 (≠ A22), S37 (= S42), G38 (= G43), S39 (≠ C44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (= T48)

3c4jA Abc protein artp in complex with atp-gamma-s
37% identity, 95% coverage: 13:253/254 of query aligns to 9:242/242 of 3c4jA

query
sites
3c4jA

L

L

D

K

A

K

Y

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

N

N

I

V

A

H

M

I

E

R

E

E

K

G

A

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

Y

F

I

D

D

-

G

-

F

-

R

-

I

-

E

E

G

G

Q

E

V

I

L

I

D

D

G

G

R

I

D

N

I

L

Y

K

A

A

A

K

D

D

V

T

R

N

V

L

D

N

E

K

L

V

R

R

K

E

E

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

S

M

I

-

F

-

E

-

N

T

V

V

V

L

Y

N

G

N

L

I

K

T

I

L

Q

A

G

P

I

M

K

K

D

V

K

R

N

K

V

W

L

P

Q

R

E

E

A

K

C

A

E

E

K

A

S

K

L

A

Q

M

H

E

A

L

L

D

W

K

N

V

E

G

V

L

K

K

D

D

K

K

L

A

D

H

K

A

S

Y

A

P

L

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

I

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

E

V

V

S

G

D

D

H

R

T

V

A

L

F

F

F

M

Y

D

M

G

G

G

E

Y

L

I

V

I

E

E

Y

E

S

G

K

K

T

P

K

E

T

D

M

L

F

F

T

D

S

R

P

P

K

Q

D

H

T

E

R

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y17), V18 (≠ A22), S38 (= S42), G39 (= G43), S40 (≠ C44), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)

3c41J Abc protein artp in complex with amp-pnp/mg2+
37% identity, 95% coverage: 13:253/254 of query aligns to 9:242/242 of 3c41J

query
sites
3c41J

L

L

D

K

A

K

Y

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

N

N

I

V

A

H

M

I

E

R

E

E

K

G

A

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

Y

F

I

D

D

-

G

-

F

-

R

-

I

-

E

E

G

G

Q

E

V

I

L

I

D

D

G

G

R

I

D

N

I

L

Y

K

A

A

A

K

D

D

V

T

R

N

V

L

D

N

E

K

L

V

R

R

K

E

E

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

S

M

I

-

F

-

E

-

N

T

V

V

V

L

Y

N

G

N

L

I

K

T

I

L

Q

A

G

P

I

M

K

K

D

V

K

R

N

K

V

W

L

P

Q

R

E

E

A

K

C

A

E

E

K

A

S

K

L

A

Q

M

H

E

A

L

L

D

W

K

N

V

E

G

V

L

K

K

D

D

K

K

L

A

D

H

K

A

S

Y

A

P

L

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

K

V

I

I

M

L

L

M

F

D
|
D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

I

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

E

V

V

S

G

D

D

H

R

T

V

A

L

F

F

F

M

Y

D

M

G

G

G

E

Y

L

I

V

I

E

E

Y

E

S

G

K

K

T

P

K

E

T

D

M

L

F

F

T

D

S

R

P

P

K

Q

D

H

T

E

R

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y17), V18 (≠ A22), S38 (= S42), G39 (= G43), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)
binding magnesium ion: S43 (= S47), D163 (= D175)

2olkA Abc protein artp in complex with adp-beta-s
37% identity, 95% coverage: 13:253/254 of query aligns to 9:242/242 of 2olkA

query
sites
2olkA

L

L

D

K

A

K

Y

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

N

N

I

V

A

H

M

I

E

R

E

E

K

G

A

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

Y

F

I

D

D

-

G

-

F

-

R

-

I

-

E

E

G

G

Q

E

V

I

L

I

D

D

G

G

R

I

D

N

I

L

Y

K

A

A

A

K

D

D

V

T

R

N

V

L

D

N

E

K

L

V

R

R

K

E

E

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

S

M

I

-

F

-

E

-

N

T

V

V

V

L

Y

N

G

N

L

I

K

T

I

L

Q

A

G

P

I

M

K

K

D

V

K

R

N

K

V

W

L

P

Q

R

E

E

A

K

C

A

E

E

K

A

S

K

L

A

Q

M

H

E

A

L

L

D

W

K

N

V

E

G

V

L

K

K

D

D

K

K

L

A

D

H

K

A

S

Y

A

P

L

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

I

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

E

V

V

S

G

D

D

H

R

T

V

A

L

F

F

F

M

Y

D

M

G

G

G

E

Y

L

I

V

I

E

E

Y

E

S

G

K

K

T

P

K

E

T

D

M

L

F

F

T

D

S

R

P

P

K

Q

D

H

T

E

R

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y17), V18 (≠ A22), S38 (= S42), G39 (= G43), S40 (≠ C44), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)

2oljA Abc protein artp in complex with adp/mg2+
37% identity, 95% coverage: 13:253/254 of query aligns to 9:242/242 of 2oljA

query
sites
2oljA

L

L

D

K

A

K

Y

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

N

N

I

V

A

H

M

I

E

R

E

E

K

G

A

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

Y

F

I

D

D

-

G

-

F

-

R

-

I

-

E

E

G

G

Q

E

V

I

L

I

D

D

G

G

R

I

D

N

I

L

Y

K

A

A

A

K

D

D

V

T

R

N

V

L

D

N

E

K

L

V

R

R

K

E

E

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

S

M

I

-

F

-

E

-

N

T

V

V

V

L

Y

N

G

N

L

I

K

T

I

L

Q

A

G

P

I

M

K

K

D

V

K

R

N

K

V

W

L

P

Q

R

E

E

A

K

C

A

E

E

K

A

S

K

L

A

Q

M

H

E

A

L

L

D

W

K

N

V

E

G

V

L

K

K

D

D

K

K

L

A

D

H

K

A

S

Y

A

P

L

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

I

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

E

V

V

S

G

D

D

H

R

T

V

A

L

F

F

F

M

Y

D

M

G

G

G

E

Y

L

I

V

I

E

E

Y

E

S

G

K

K

T

P

K

E

T

D

M

L

F

F

T

D

S

R

P

P

K

Q

D

H

T

E

R

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding adenosine-5'-diphosphate: F13 (≠ Y17), V18 (≠ A22), S38 (= S42), G39 (= G43), S40 (≠ C44), G41 (= G45), K42 (= K46), S43 (= S47), T44 (= T48)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
37% identity, 93% coverage: 14:250/254 of query aligns to 6:237/240 of 4ymuJ

query
sites
4ymuJ

D

D

A

V

Y

Y

-

K

-

N

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

V

N

T

I

L

A

K

M

V

E

N

E

K

K

G

A

E

V

V

T

V

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

T

C

L

I

N

N

R

L

M

L

N

E

D

E

Y

-

I

-

D

-

G

-

F

-

R

P

I

T

E

K

G

G

Q

E

V

V

L

F

L

I

D

D

G

G

R

V

D

K

I

I

Y

N

A

N

A

G

D

K

V

V

R

N

V

I

D

N

E

K

L

V

R

R

K

Q

E

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

F

P

P

K

H

-

L

S

T

I

A

F

I

E

E

N

N

V

I

V

T

Y

L

G

A

-

P

L

V

K

K

I

V

Q

K

G

K

I

M

K

N

D

K

K

K

N

E

V

A

L

-

Q

E

E

E

A

L

C

A

E

V

K

D

S

L

L

L

Q

A

H

K

A

V

A

G

L

L

W

L

N

-

E

-

V

-

K

-

D

D

K

K

L

K

D

D

K

Q

S

Y

A

P

L

I

A

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

Q

P

P

S

E

V

V

I

M

L

L

M

F

D

D

E
|
E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

K

K

E

I

V

E

L

D

N

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H
|
H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

E

V

V

S

G

D

D

H

R

T

V

A

I

F

F

F

M

Y

D

M

D

G

G

E

V

L

I

V

V

E

E

Y

E

S

G

K

T

T

P

K

E

T

E

M

I

F

F

T

Y

S

R

P

A

K

K

D

N

T

E

R

R

T

T

Q

R

N

E

Y

F

I

L

T

S

binding adenosine-5'-triphosphate: F11 (≠ Y17), V16 (≠ A22), S36 (= S42), G37 (= G43), S38 (≠ C44), G39 (= G45), K40 (= K46), S41 (= S47), T42 (= T48), E162 (= E176), H194 (= H207)
binding magnesium ion: S41 (= S47), E162 (= E176)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 88% coverage: 27:249/254 of query aligns to 46:263/382 of 7ahhC

query
sites
7ahhC

N

N

I

F

A

E

M

I

E

N

E

E

K

G

A

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

T

L

L

L

N

N

R

R

M

L

N

I

D

E

Y

P

I

T

D

-

G

-

F

-

R

-

I

-

E

S

G

G

Q

K

V

I

L

F

L

I

D

D

G

N

R

Q

D

D

I

V

Y

-

A

-

A

A

D

T

V

L

R

N

V

K

D

E

E

D

L

L

-

L

-

Q

-

V

-

R

R

R

K

K

E

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

E

E

N

N

V

T

V

E

Y

Y

G

G

L

L

K

E

I

V

Q

Q

G

N

I

V

K

P

D

-

K

K

N

E

V

E

L

R

Q

R

E

K

A

R

C

A

E

E

K

K

S

A

L

L

Q

D

H

N

A

A

A

N

L

L

W

L

N

-

E

D

V

F

K

K

D

D

K

Q

L

Y

D

P

K

K

S

Q

A

-

L

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

L

L

A

A

I

N

Q

D

P

P

S

E

V

I

I

L

L

L

M

M

D
|
D

E
|
E

P

A

A

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

A

R

K

E

I

M

E

Q

D

D

L

E

I

L

H

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

G

L

R

R

V

I

S

G

D

D

H

R

T

I

A

A

F

I

F

M

Y

K

M

D

G

G

E

K

L

I

V

M

E

Q

Y

I

S

G

K

T

T

G

K

E

T

E

M

I

F

L

T

T

S

N

P

P

K

A

D

N

T

D

R

Y

T

V

Q

K

N

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G43), G64 (= G45), K65 (= K46), D187 (= D175), E188 (= E176)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 88% coverage: 27:249/254 of query aligns to 46:263/382 of 7aheC

query
sites
7aheC

N

N

I

F

A

E

M

I

E

N

E

E

K

G

A

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

F

L

L

L

R

R

T

L

L

L

N

N

R

R

M

L

N

I

D

E

Y

P

I

T

D

-

G

-

F

-

R

-

I

-

E

S

G

G

Q

K

V

I

L

F

L

I

D

D

G

N

R

Q

D

D

I

V

Y

-

A

-

A

A

D

T

V

L

R

N

V

K

D

E

E

D

L

L

-

L

-

Q

-

V

-

R

R

R

K

K

E

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

E

E

N

N

V

T

V

E

Y

Y

G

G

L

L

K

E

I

V

Q

Q

G

N

I

V

K

P

D

-

K

K

N

E

V

E

L

R

Q

R

E

K

A

R

C

A

E

E

K

K

S

A

L

L

Q

D

H

N

A

A

A

N

L

L

W

L

N

-

E

D

V

F

K

K

D

D

K

Q

L

Y

D

P

K

K

S

Q

A

-

L

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

L

L

A

A

I

N

Q

D

P

P

S

E

V

I

I

L

L

L

M

M

D

D

E

E

P

A

A

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

A

R

K

E

I

M

E

Q

D

D

L

E

I

L

H

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

G

L

R

R

V

I

S

G

D

D

H

R

T

I

A

A

F

I

F

M

Y

K

M

D

G

G

E

K

L

I

V

M

E

Q

Y

I

S

G

K

T

T

G

K

E

T

E

M

I

F

L

T

T

S

N

P

P

K

A

D

N

T

D

R

Y

T

V

Q

K

N

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
35% identity, 84% coverage: 27:240/254 of query aligns to 46:254/260 of 7ahdC

query
sites
7ahdC

N

N

I

F

A

E

M

I

E

N

E

E

K

G

A

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

T

L

L

L

N

N

R

R

M

L

N

I

D

E

Y

P

I

T

D

S

G

G

F

-

R

-

I

-

E

-

G

-

Q

K

V

I

L

F

L

I

D

D

G

N

R

Q

D

D

I

V

Y

-

A

-

A

A

D

T

V

L

R

N

V

K

D

E

E

D

L

L

-

L

-

Q

-

V

-

R

R

R

K

K

E

T

V

M

G

S

M

M

V

V

F

F

Q
|
Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

E

E

N

N

V

T

V

E

Y

Y

G

G

L

L

K

E

I

V

Q

Q

G

N

I

V

K

P

D

-

K

K

N

E

V

E

L

R

Q

R

E

K

A

R

C

A

E

E

K

K

S

A

L

L

Q

D

H

N

A

A

A

N

L

L

W

L

N

-

E

D

V

F

K

K

D

D

K

Q

L

Y

D

P

K
|
K

S
x
Q

A

-

L

-

A

-

L

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

L

L

A

A

I

N

Q

D

P

P

S

E

V

I

I

L

L

L

M

M

D

D

E
x
Q

P

A

A

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

A

R

K

E

I

M

E

Q

D

D

L

E

I

L

H

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H
|
H

N

D

M

L

Q

N

Q

E

A

A

G

L

R

R

V

I

S

G

D

D

H

R

T

I

A

A

F

I

F

M

Y

K

M

D

G

G

E

K

L

I

V

M

E

Q

Y

I

S

G

K

T

T

G

K

E

T

E

M

I

F

L

T

T

S

N

P

P

binding adenosine-5'-triphosphate: S61 (= S42), G62 (= G43), G64 (= G45), K65 (= K46), S66 (= S47), T67 (= T48), Q111 (= Q95), K161 (= K146), Q162 (≠ S147), S164 (= S152), G166 (= G154), M167 (≠ Q155), Q188 (≠ E176), H221 (= H207)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 91% coverage: 20:249/254 of query aligns to 18:241/343 of P30750

query
sites
P30750

F

I

H

Q

A

A

L

L

K

N

G

N

I

V

N

S

I

L

A

H

M

V

E

P

E

A

K

G

A

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

Y

-

I

-

D

-

G

-

F

-

R

P

I

T

E

E

G

G

Q

S

V

V

L

L

L

V

D

D

G

G

R

Q

D

E

I

L

Y

T

A

T

-

L

A

S

D

E

V

S

R

E

V

L

D

T

E

K

L

A

R

R

K

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

V

A

Y

L

G

P

L

L

K

E

I

L

Q

D

G

-

I

-

K

-

D

-

K

-

N

N

V

T

L

P

Q

K

E

D

A

E

C

V

E

K

K

R

S

R

L

V

Q

T

H

E

A

L

L

S

W

L

N

V

E

G

V

L

K

G

D

D

K

K

L

H

D

D

K

S

S

Y

A

P

L

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

S

Q

N

P

P

S

K

V

V

I

L

L

L

M

C

D

D

E
|
E

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

G

K

R

R

V

I

S

C

D

D

H

C

T

V

A

A

F

V

F

I

Y

S

M

N

G

G

E

E

L

L

V

I

E

E

Y

Q

S

D

K

T

T

V

K

S

T

E

M

V

F

F

T

S

S

H

P

P

K

K

D

T

T

P

R

L

T

A

Q

Q

N

K

Y

F

I

I

40:46 (vs. 42:48, 86% identical) binding ATP
E166 (= E176) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 91% coverage: 20:249/254 of query aligns to 19:242/344 of 6cvlD

query
sites
6cvlD

F
x
I

H

Q

A

A

L

L

K

N

G

N

I

V

N

S

I

L

A

H

M

V

E

P

E

A

K

G

A

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

Y

-

I

-

D

-

G

-

F

-

R

P

I

T

E

E

G

G

Q

S

V

V

L

L

L

V

D

D

G

G

R

Q

D

E

I

L

Y

T

A

T

-

L

A

S

D

E

V

S

R

E

V

L

D

T

E

K

L

A

R

R

K

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

V

A

Y

L

G

P

L

L

K

E

I

L

Q

-

G

-

I

-

K

-

D

-

K

D

N

N

V

T

L

P

Q

K

E

D

A

E

C

V

E

K

K

R

S

R

L

V

Q

T

H

E

A

L

L

S

W

L

N

V

E

G

V

L

K

G

D

D

K

K

L

H

D

D

K

S

S

Y

A

P

L

S

A
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

S

Q

N

P

P

S

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H
|
H

N

E

M

M

Q

D

Q

V

A

V

G

K

R

R

V

I

S

C

D

D

H

C

T

V

A

A

F

V

F

I

Y

S

M

N

G

G

E

E

L

L

V

I

E

E

Y

Q

S

D

K

T

T

V

K

S

T

E

M

V

F

F

T

S

S

H

P

P

K

K

D

T

T

P

R

L

T

A

Q

Q

N

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ F20), S41 (= S42), G42 (= G43), A43 (≠ C44), G44 (= G45), K45 (= K46), S46 (= S47), T47 (= T48), N141 (≠ A150), S143 (= S152), Q146 (= Q155), H200 (= H207)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
32% identity, 91% coverage: 20:249/254 of query aligns to 19:242/344 of 3tuzC

query
sites
3tuzC

F

I

H

Q

A

A

L

L

K

N

G

N

I

V

N

S

I

L

A

H

M

V

E

P

E

A

K

G

A

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

Y

-

I

-

D

-

G

-

F

-

R

P

I

T

E

E

G

G

Q

S

V

V

L

L

L

V

D

D

G

G

R

Q

D

E

I

L

Y

T

A

T

-

L

A

S

D

E

V

S

R

E

V

L

D

T

E

K

L

A

R

R

K

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

V

A

Y

L

G

P

L

L

K

E

I

L

Q

-

G

-

I

-

K

-

D

-

K

D

N

N

V

T

L

P

Q

K

E

D

A

E

C

V

E

K

K

R

S

R

L

V

Q

T

H

E

A

L

L

S

W

L

N

V

E

G

V

L

K

G

D

D

K

K

L

H

D

D

K

S

S

Y

A

P

L

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

S

Q

N

P

P

S

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

G

K

R

R

V

I

S

C

D

D

H

C

T

V

A

A

F

V

F

I

Y

S

M

N

G

G

E

E

L

L

V

I

E

E

Y

Q

S

D

K

T

T

V

K

S

T

E

M

V

F

F

T

S

S

H

P

P

K

K

D

T

T

P

R

L

T

A

Q

Q

N

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G43), G44 (= G45), K45 (= K46), S46 (= S47), T47 (= T48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
32% identity, 91% coverage: 20:249/254 of query aligns to 19:242/344 of 3tuiC

query
sites
3tuiC

F

I

H

Q

A

A

L

L

K

N

G

N

I

V

N

S

I

L

A

H

M

V

E

P

E

A

K

G

A

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

Y

-

I

-

D

-

G

-

F

-

R

P

I

T

E

E

G

G

Q

S

V

V

L

L

L

V

D

D

G

G

R

Q

D

E

I

L

Y

T

A

T

-

L

A

S

D

E

V

S

R

E

V

L

D

T

E

K

L

A

R

R

K

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

V

A

Y

L

G

P

L

L

K

E

I

L

Q

-

G

-

I

-

K

-

D

-

K

D

N

N

V

T

L

P

Q

K

E

D

A

E

C

V

E

K

K

R

S

R

L

V

Q

T

H

E

A

L

L

S

W

L

N

V

E

G

V

L

K

G

D

D

K

K

L

H

D

D

K

S

S

Y

A

P

L

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

L

L

A

A

I

S

Q

N

P

P

S

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

G

K

R

R

V

I

S

C

D

D

H

C

T

V

A

A

F

V

F

I

Y

S

M

N

G

G

E

E

L

L

V

I

E

E

Y

Q

S

D

K

T

T

V

K

S

T

E

M

V

F

F

T

S

S

H

P

P

K

K

D

T

T

P

R

L

T

A

Q

Q

N

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G43), A43 (≠ C44), G44 (= G45), K45 (= K46), S46 (= S47), T47 (= T48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3d31A Modbc from methanosarcina acetivorans (see paper)
30% identity, 95% coverage: 8:249/254 of query aligns to 2:228/348 of 3d31A

query
sites
3d31A

L

I

E

E

A

I

I

E

N

S

L

L

D

S

A

R

Y

K

Y

W

G

K

D

N

F

F

H

-

A

S

L

L

K

D

G

N

I

L

N

S

I

L

A

K

M

V

E

E

E

S

K

G

A

E

V

Y

T

F

A

V

F

I

I

L

G

G

P

P

S

T

G

G

C

A

G

G

K

K

S

T

T

L

F

F

L

L

R

-

T

-

L

-

N

-

R

-

M

-

N

-

D

E

Y

L

I

I

D

A

G

G

F

F

R

H

I

V

-

P

-

D

E

S

G

G

Q

R

V

I

L

L

D

D

G

G

R

K

D

D

I

V

Y

-

A

-

A

T

D

D

V

L

R

S

V

P

D

E

E

-

L

-

R

K

K

H

E

D

V

I

G

A

M

F

V

V

F

Y

Q

Q

K

N

P

Y

N

S

P

L

F

F

P

P

K

H

-

M

S

N

I

V

F

K

E

K

N

N

V

L

V

E

Y

F

G

G

L

M

K

R

I

M

Q

K

G

K

I

I

K

K

D

D

-

P

K

K

N

R

V

V

L

L

Q

D

E

T

A

A

C

R

E

D

K

L

S

K

L

I

Q

E

H

H

A

L

A

-

L

-

W

-

N

-

E

-

V

-

K

-

D

-

K

-

L

L

D

D

K

R

S

N

A

P

L

L

A

T

L

L

S

S

G

G

Q

E

Q

Q

R

R

L

V

C

A

I

L

A

A

R

R

A

A

L

L

A

V

I

T

Q

N

P

P

S

K

V

I

I

L

L

L

M

L

D

D

E

E

P

P

A

L

S

S

A

A

L

L

D

D

P

P

I

R

S

T

T

Q

A

E

K

N

I

A

E

R

D

E

L

M

I

L

H

S

E

V

L

L

-

H

-

K

K

K

K

N

D

K

Y

L

T

T

I

V

V

L

I

H

V

I

T

T

H

H

N

D

M

Q

Q

T

Q

E

A

A

G

R

R

I

V

M

S

A

D

D

H

R

T

I

A

A

F

V

Y

M

M

D

G

G

E

K

L

L

V

I

E

Q

Y

V

S

G

K

K

T

P

K

E

T

E

M

I

F

F

T

E

S

K

P

P

K

V

D

E

T

G

R

R

T

V

Q

A

N

S

Y

F

I

V

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
35% identity, 97% coverage: 6:251/254 of query aligns to 1:251/258 of 1b0uA

query
sites
1b0uA

S

N

K

K

L

L

E

H

A

V

I

I

N

D

L

L

D

H

A

K

Y

R

Y
|
Y

G

G

D

G

F
x
H

H

E

A

V

L

L

K

K

G

G

I

V

N

S

I

L

A

Q

M

A

E

R

E

A

K

G

A

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

I

N

N

R

F

M

L

N

E

D

K

Y

P

I

S

D

E

G

G

-

A

F

I

R

I

I

V

E

N

G

G

Q

Q

V

N

L

I

-

N

-

L

-

V

-

R

-

D

L

K

D

D

G

G

R

Q

D

L

I

K

Y

V

A

A

A

D

D

K

V

N

R

Q

V

L

D

R

E

L

L

L

R

R

K

T

E

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

F

L

E

E

N

N

V

V

V

M

Y

E

G

A

-

P

L

I

K

Q

I

V

Q

L

G

G

I

L

K

S

D

K

K

-

N

-

V

-

L

-

Q

H

E

D

A

A

C

R

E

E

K

R

S

A

L

L

Q

K

H

Y

A

L

A

A

L

K

W

V

N

G

E

I

V

D

K

E

D

R

K

A

L

Q

D

G

K

K

S

Y

A

P

L

V

A

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

D

V

V

I

L

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

L

T

V

A

G

K

E

I

V

E

L

D

R

L

I

I

M

H

Q

E

Q

L

L

K

A

K

E

D

E

-

G

Y

K

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

H

V

V

S

S

D

S

H

H

T

V

A

I

F

F

F

L

Y

H

M

Q

G

G

E

K

L

I

V

E

E

E

Y

E

S

G

K

D

T

P

K

E

T

Q

M

V

F

F

T

G

S

N

P

P

K

Q

D

S

T

P

R

R

T

L

Q

Q

N

Q

Y

F

I

L

T

K

G

G

binding adenosine-5'-triphosphate: Y12 (= Y17), H15 (≠ F20), S37 (= S42), G38 (= G43), G40 (= G45), K41 (= K46), S42 (= S47), T43 (= T48)

P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 97% coverage: 6:251/254 of query aligns to 5:255/258 of P02915

query
sites
P02915

S

N

K

K

L

L

E

H

A

V

I

I

N

D

L

L

D

H

A

K

Y

R

Y

Y

G

G

D

G

F

H

H

E

A

V

L

L

K

K

G

G

I

V

N

S

I

L

A

Q

M

A

E

R

E

A

K

G

A

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

I

N

N

R

F

M

L

N

E

D

K

Y

P

I

S

D

E

G

G

-

A

F

I

R

I

I

V

E

N

G

G

Q

Q

V

N

L

I

-

N

-

L

-

V

-

R

-

D

L

K

D

D

G

G

R

Q

D

L

I

K

Y

V

A

A

A

D

D

K

V

N

R

Q

V

L

D

R

E

L

L

L

R

R

K

T

E

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

F

L

E

E

N

N

V

V

V

M

Y

E

G

A

-

P

L

I

K

Q

I

V

Q

L

G

G

I

L

K

S

D

K

K

-

N

-

V

-

L

-

Q

H

E

D

A

A

C

R

E

E

K

R

S

A

L

L

Q

K

H

Y

A

L

A

A

L

K

W

V

N

G

E

I

V

D

K

E

D

R

K

A

L

Q

D

G

K

K

S

Y

A

P

L

V

A

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

L

L

A

A

I

M

Q

E

P

P

S

D

V

V

I

L

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

L

T

V

A

G

K

E

I

V

E

L

D

R

L

I

I

M

H

Q

E

Q

L

L

K

A

K

E

D

E

-

G

Y

K

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

G

R

R

H

V

V

S

S

D

S

H

H

T

V

A

I

F

F

F

L

Y

H

M

Q

G

G

E

K

L

I

V

E

E

E

Y

E

S

G

K

D

T

P

K

E

T

Q

M

V

F

F

T

G

S

N

P

P

K

Q

D

S

T

P

R

R

T

L

Q

Q

N

Q

Y

F

I

L

T

K

G

G

S41 (= S42) binding ATP
G42 (= G43) binding ATP
G44 (= G45) binding ATP
K45 (= K46) binding ATP
S46 (= S47) binding ATP
T47 (= T48) binding ATP

7qkrA Cryo-em structure of abc transporter ste6-2p from pichia pastoris with verapamil at 3.2 a resolution (see paper)
31% identity, 86% coverage: 19:237/254 of query aligns to 376:595/1199 of 7qkrA

query
sites
7qkrA

D

D

F

I

H

T

A
x
V

L

L

K

S

G

D

I

F

N

T

I

L

A

D

M

I

E

K

E

P

K

G

A

Q

V

T

T

I

A

A

F

L

I

V

G

G

P

E

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

V

L

I

R

A

T

L

L

L

N

E

R

R

M

F

N

Y

D

E

Y

Y

I

L

D

D

G

G

F

-

R

-

I

-

E

-

G

-

Q

E

V

I

L

L

D

D

G

G

R

V

D

D

I

L

Y

K

A

S

A

L

D

N

V

I

R

K

V

-

D

-

E

W

L

V

R

R

K

Q

E

Q

V

M

G

A

M

L

V

V

F

Q

Q

Q

K

E

P

P

N

V

P

L

F

F

P

A

K

A

S

S

I

I

F

Y

E

E

N

N

V

V

V

C

Y

Y

G

G

L

L

-

V

-

G

-

S

K

K

I

Y

Q

E

G

N

I

V

K

T

D

E

K

K

N

-

V

V

L

K

Q

R

E

E

A

L

C

V

E

E

K

K

S

A

L

C

Q

K

H

D

A

A

A

N

L

A

W

W

-

E

-

F

-

I

-

S

-

Q

-

M

-

S

N

N

E

G

V

L

K

D

D

T

K

E

L

V

D

G

K

E

S

R

A

G

L

L

A

S

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

L

V

A

I

I

S

Q

E

P

P

S

K

V

I

I

L

L

L

M

L

D

D

E

E

P

A

A

T

S

S

A

A

L

L

D

D

P

T

I

R

S

S

T

E

A

G

K

I

I

V

E

Q

D

D

L

A

I

L

H

N

E

R

L

L

K

S

K

E

D

T

Y

R

T

T

I

T

V

I

I

V

V

I

T

A

H

H

N

R

M

L

Q

S

Q

T

A

I

G

-

R

Q

V

N

S

A

D

D

H

L

T

I

A

V

F

V

F

L

Y

S

M

K

G

G

E

K

L

I

V

V

E

E

Y

T

S

G

K

S

T

H

K

K

T

E

M

L

F

L

binding phosphoaminophosphonic acid-adenylate ester: V379 (≠ A22), S399 (= S42), G400 (= G43), S401 (≠ C44), G402 (= G45), K403 (= K46), S404 (= S47), T405 (= T48)

Sites not aligning to the query:

6qexA Nanodisc reconstituted human abcb1 in complex with uic2 fab and taxol (see paper)
34% identity, 79% coverage: 19:218/254 of query aligns to 955:1160/1182 of 6qexA

query
sites
6qexA

D

D

F

I

H

P

A

V

L

L

K

Q

G

G

I

L

N

S

I

L

A

E

M

V

E

K

K

G

A

Q

V

T

T

L

A

A

F

L

I

V

G

G

P

S

S

S

G

G

C

C

G

G

K

K

S

S

T

T

F

V

L

V

R

Q

T

L

L

L

N

E

R

R

M

F

N

Y

D

D

Y

-

I

-

D

-

G

-

F

-

R

P

I

L

E

A

G

G

Q

K

V

V

L

L

D

D

G

G

R

K

D

E

I

I

Y

-

A

-

A

K

D

R

V

L

R

N

V

V

D

Q

E

W

L

L

R

R

K

A

E

H

V

L

G

G

M

I

V

V

F

S

Q

Q

K

E

P

P

N

I

P

L

F

F

P

D

K

C

S

S

I

I

F

A

E

E

N

N

V

I

V

A

Y

Y

G

G

L

D

K

N

I

S

Q

R

G

V

I

V

K

S

D

Q

K

E

N

E

V

I

L

V

Q

R

E

A

A

A

C

K

E

E

K

A

S

N

L

I

Q

-

H

H

A

A

-

F

-

I

-

E

A

S

L

L

W

P

N

N

E

K

V

Y

K

S

D

T

K

K

L

V

D

G

K

D

S

K

A

G

L

T

A

Q

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

L

L

A

V

I

R

Q

Q

P

P

S

H

V

I

I

L

L

L

M

L

D

D

E

E

P

A

A

T

S

S

A

A

L

L

D

D

P

T

I

E

S

S

T

E

A

K

K

V

I

V

E

Q

D

E

L

A

I

L

H

D

E

K

L

A

K

R

K

E

D

G

Y

R

T

T

I

C

V

I

I

V

V

I

T

A

H

H

N

R

M

L

Q

S

-

T

-

I

-

Q

-

N

-

A

-

D

-

L

-

I

-

V

-

F

Q

Q

A

N

G

G

R

R

V

V

S

K

D

E

H

H

Sites not aligning to the query:

binding cholesterol: 15, 18, 36, 43, 81, 86, 89, 189, 281, 745, 749, 751, 755, 757, 761, 762, 765, 769, 772, 775, 854, 857, 861, 864, 865
binding taxol: 38, 276, 279, 305, 312, 313, 316, 631, 852, 859, 889, 892, 896

7o9wA Encequidar-bound human p-glycoprotein in complex with uic2-fab (see paper)
34% identity, 79% coverage: 19:218/254 of query aligns to 942:1147/1169 of 7o9wA

query
sites
7o9wA

D

D

F

I

H

P

A

V

L

L

K

Q