SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_101445098.1 NCBI__GCF_002846395.1:WP_101445098.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
41% identity, 97% coverage: 3:194/198 of query aligns to 539:727/758 of O14289

query
sites
O14289

K

K

F

F

E

T

I

V

R

E

T

G

G

I

A

A

V

A

P

P

L

L

P

P

I

M

E

A

N

N

I

V

D

D

T

T

D

D

Q

K

I

I

P

P

A

K

R

Q

F

F

L

L

K

K

A

T

T

I

S

K

R

R

E

T

G

G

F

L

G

G

E

Q

N

F

L

A

F

F

R

Y

D

E

W

I

R

R

Y

Y

S

D

S

A

S

D

G

G

E

K

P

E

K

I

R

P

D

D

F

F

V

V

L

L

N

N

N

R

S

E

R

P

Y

Y

K

R

-

H

G

A

Q

T

V

V

L

L

L

V

A

A

G

H

K

D

N

N

F

F

G

G

C

C

G

G

S

S

R

R

E

E

H

H

A

A

A

P

W

W

A

A

I

L

Y

N

D

D

A

F

G

G

F

I

R

R

V

V

V

I

I

I

S

A

S

P

Y

S

F

F

A

A

D

D

I

I

F

F

R

F

G

N

N

N

A

C

L

F

N

K

N

N

G

G

L

M

L

L

P

P

L

I

Q

P

V

T

T

P

D

I

E

E

V

Q

L

V

Q

N

R

D

L

M

F

-

A

-

L

M

V

K

E

A

A

A

D

E

P

N

Q

Q

T

V

E

K

F

F

V

S

I

V

N

D

L

L

P

V

E

N

Q

Q

E

T

L

I

Y

T

L

Y

P

-

A

-

S

G

G

D

E

K

R

Q

I

V

P

K

F

F

A

D

I

V

D

E

P

P

Y

F

K

R

K

K

E

H

C

C

L

L

I

V

K

N

G

G

Y

L

D

D

I

I

D

G

F

L

L

T

V

L

S

Q

Q

K

K

E

E

T

A

M

I

I

T

D

A

A

Y

F

E

E

S

A

T

A

R

R

Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 99% coverage: 1:196/198 of query aligns to 1:189/198 of P9WK95

query
sites
P9WK95

M

M

E

E

K

A

F

F

E

H

I

T

I

H

R

S

T

G

G

I

A

G

V

V

P

P

L

L

P

R

I

R

E

S

N

N

I

V

D

D

T

T

D

D

Q

Q

I

I

P

P

A

A

R

V

F

F

L

L

K

K

A

R

T

V

S

T

R

R

E

T

G

G

F

F

G

E

E

D

N

G

L

L

F

F

R

A

D

G

W

W

R

R

Y

S

S

D

S

P

S

A

G

-

E

-

P

-

K

-

R

-

D

-

F

F

V

V

L

L

N

N

N

L

S

S

R

P

Y

F

-

D

K

R

G

G

Q

S

V

V

L

L

L

V

A

A

G

G

K

P

N

D

F

F

G

G

C

T

G

G

S

S

R

R

E

E

H

H

A

A

A

V

W

W

A

A

I

L

Y

M

D

D

A

Y

G

G

F

F

R

R

V

V

I

I

S

S

Y

R

F

F

A

G

D

D

I

I

F

F

R

R

G

G

N

N

A

A

L

G

N

K

N

A

G

G

L

L

P

A

L

A

Q

E

V

V

T

A

D

Q

E

D

V

D

L

V

Q

E

R

L

L

L

F

W

A

K

L

L

V

I

E

E

A

Q

D

S

P

P

Q

G

T

L

E

E

F

I

V

T

I

A

N

N

L

L

P

-

E

-

Q

Q

E

D

L

R

Y

I

L

I

P

T

A

A

S

A

G

T

E

V

R

V

I

L

P

P

F

F

A
x
K

I

I

D

D

P

D

Y

H

K

S

K

A

E

W

C

R

L

L

I

L

K

E

G

G

Y

L

D

D

I

I

D

A

F

L

L

T

V

L

S

R

Q

K

K

L

E

D

A

E

I

I

T

E

A

A

Y

F

E

E

S

G

T

A

R

C

A

A

W

Y

K154 (≠ A161) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
34% identity, 64% coverage: 17:143/198 of query aligns to 12:129/167 of 2pkpA

query
sites
2pkpA

E

D

N
x
D

I

V

D

D

T

T

D

D

Q

A

I

I

P

P

A

G

R

P

F

Y

L

L

K

R

A

T

T

T

S

D

R

-

E

-

G

-

F

-

G

-

E

-

N

-

L

-

F

-

R

-

D

P

W

Y

R

E

Y

L

S

A

S

S

S

H

G

C

E

M

P

A

K

G

R

I

D

D

F

E

V

N

L

F

N

P

N

K

S

K

R

V

Y

K

K

E

G

G

Q

D

V

V

L

I

L

V

A

A

G

G

K

E

N

N

F

F

G

G

C
|
C

G

G

S

S

R

R

E

E

H

Q

A

A

A

V

W

I

A

A

I

I

Y

K

D

Y

A

C

G

G

F

I

R

K

V

A

V

V

I

I

S

A

S

K

Y

S

F

F

A

A

D

R

I

I

F

F

R

Y

G

R

N

N

A

A

L

I

N

N

N

V

G

G

L

L

L

I

P

P

L

I

Q

I

V

A

-

N

T

T

D

D

E

E

V

I

L

K

Q

D

R

G

L

D

F

I

A

V

L

E

V

I

E

D

A

L

D

D

P

K

Q

E

T

E

E

I

F

V

V

I

I

T

N

N

binding zinc ion: D13 (≠ N18), C63 (= C78)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
34% identity, 64% coverage: 17:143/198 of query aligns to 12:129/170 of Q58667

query
sites
Q58667

E

D

N

D

I

V

D

D

T

T

D

D

Q

A

I

I

P

P

A

G

R

P

F

Y

L

L

K
x
R

A
x
T

T

T

S

D

R

-

E

-

G

-

F

-

G

-

E

-

N

-

L

-

F

-

R

-

D

P

W

Y

R

E

Y

L

S

A

S

S

S

H

G

C

E

M

P

A

K

G

R

I

D

D

F

E

V

N

L

F

N

P

N

K

S

K

R

V

Y

K

K

E

G

G

Q

D

V

V

L

I

L

V

A

A

G

G

K

E

N

N

F

F

G

G

C

C

G

G

S

S

R

R

E

E

H

Q

A

A

A

V

W

I

A

A

I

I

Y

K

D

Y

A

C

G

G

F

I

R

K

V

A

V

V

I

I

S

A

S

K

Y

S

F

F

A

A

D

R

I

I

F

F

R

Y

G

R

N

N

A

A

L

I

N

N

N

V

G

G

L

L

L

I

P

P

L

I

Q

I

V

A

-

N

T

T

D

D

E

E

V

I

L

K

Q

D

R

G

L

D

F

I

A

V

L

E

V

I

E

D

A

L

D

D

P

K

Q

E

T

E

E

I

F

V

V

I

I

T

N

N

R26 (≠ K31) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (≠ A32) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 2 papers)
49% identity, 27% coverage: 67:119/198 of query aligns to 779:831/909 of P09339

query
sites
P09339

G

G

Q

L

V

V

L

V

L

L

A

A

G

G

K

K

N

D

F

Y

G

G

C

M

G

G

S

S

R

R

E

D

H

W

A

A

W

K

A

G

I

T

Y

N

D

L

A

L

G

G

F

I

R

R

V

T

V

V

I

I

S

A

S

E

Y

S

F

F

A

E

D

R

I

I

F

H

R

R

G

S

N

N

A

L

L

V

N

F

N

M

G

G

L

V

L

L

P

P

L

L

Q

Q

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
450 C→S: Loss of aconitase activity. It is glutamate auxotroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of inactive aconitase.
741 R→E: Same aconitase activity compared to the wild-type. It is glutamate prototroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of active aconitase.

P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see 2 papers)
44% identity, 27% coverage: 69:122/198 of query aligns to 658:711/780 of P20004

query
sites
P20004

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S

S

S
|
S

R
|
R

E

E

H

H

A

A

W

L

A

E

I

P

Y

R

D

H

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

SR 670:671 (= SR 81:82) binding substrate

Sites not aligning to the query:

99 binding substrate
192:194 binding substrate
385 binding [4Fe-4S] cluster
448 binding [4Fe-4S] cluster
451 binding [4Fe-4S] cluster
474 binding substrate
479 binding substrate
607 binding substrate

D9X0I3 Aconitate hydratase A; ACN; Aconitase; EC 4.2.1.3 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see paper)
45% identity, 26% coverage: 69:119/198 of query aligns to 805:855/931 of D9X0I3

query
sites
D9X0I3

V

V

L

V

L

L

A

A

G

G

K

K

N

E

F

Y

G

G

C

S

G

G

S

S

R

R

E

D

H

W

A

A

W

K

A

G

I

T

Y

A

D

L

A

L

G

G

F

V

R

K

V

A

V

V

I

I

S

A

S

E

Y

S

F

Y

A

E

D

R

I

I

F

H

R

R

G

S

N

N

A

L

L

I

N

G

N

M

G

G

L

V

L

L

P

P

L

L

Q

Q

Sites not aligning to the query:

125:129 mutation Missing: Retains 40% of aconitase activity. Improves RNA-binding ability.
538 C→A: Loss of aconitase activity. Cannot rescue the growth defect of a disruption mutant and results in only a slight increase in PTT production in the mutant. Shows weak IRE-binding activity.
763 R→E: Loss of aconitase activity and IRE-binding activity; when associated with E-767.
767 Q→E: Loss of aconitase activity and IRE-binding activity; when associated with E-763.

5acnA Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
39% identity, 32% coverage: 59:122/198 of query aligns to 616:684/754 of 5acnA

query
sites
5acnA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

S

S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

H

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D
x
R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: S642 (= S80), R644 (= R82)
binding tricarballylic acid: R666 (≠ D104)

Sites not aligning to the query:

active site: 100, 101, 165, 447
binding fe3-s4 cluster: 145, 147, 167, 358, 421, 424, 446
binding tricarballylic acid: 198, 235

A0QX20 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
43% identity, 26% coverage: 69:119/198 of query aligns to 811:861/943 of A0QX20

query
sites
A0QX20

V

V

L

V

L

L

A

G

G

G

K

K

N

E

F

Y

G

G

C

S

G

G

S

S

R

R

E

D

H

W

A

A

W

K

A

G

I

T

Y

V

D

L

A

L

G

G

F

V

R

K

V

A

V

V

I

I

S

T

S

E

Y

S

F

F

A

E

D

R

I

I

F

H

R

R

G

S

N

N

A

L

L

I

N

G

N

M

G

G

L

V

L

I

P

P

L

L

Q

Q

Sites not aligning to the query:

394 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P16276 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Sus scrofa (Pig) (see 3 papers)
39% identity, 32% coverage: 59:122/198 of query aligns to 643:711/781 of P16276

query
sites
P16276

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S

S

S
|
S

R
|
R

E

E

H

H

A

R

A

A

W

L

A

E

I

P

Y

R

D

H

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

SR 670:671 (= SR 81:82) binding substrate

Sites not aligning to the query:

28 modified: Pyrrolidone carboxylic acid
99 binding substrate
192:194 binding substrate
385 binding [4Fe-4S] cluster
448 binding [4Fe-4S] cluster
451 binding [4Fe-4S] cluster
474 binding substrate
479 binding substrate
607 binding substrate

1b0kA S642a:fluorocitrate complex of aconitase (see paper)
38% identity, 32% coverage: 59:122/198 of query aligns to 615:683/753 of 1b0kA

query
sites
1b0kA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
x
A

S
|
S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

H

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: A641 (≠ S80), R643 (= R82)
binding citrate anion: A641 (≠ S80), S642 (= S81), R643 (= R82)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding citrate anion: 71, 100, 164, 165, 446, 451, 579
binding oxygen atom: 164, 166
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423

8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound (see paper)
39% identity, 32% coverage: 59:122/198 of query aligns to 615:683/753 of 8acnA

query
sites
8acnA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

F

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: S641 (= S80), R643 (= R82)
binding nitroisocitric acid: S641 (= S80), S642 (= S81), R643 (= R82)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding nitroisocitric acid: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423, 424

1fghA Complex with 4-hydroxy-trans-aconitate (see paper)
39% identity, 32% coverage: 59:122/198 of query aligns to 615:683/753 of 1fghA

query
sites
1fghA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

F

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: S641 (= S80), R643 (= R82)
binding 4-hydroxy-aconitate ion: S641 (= S80), S642 (= S81), R643 (= R82)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding 4-hydroxy-aconitate ion: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423, 424, 451

1amjA Steric and conformational features of the aconitase mechanism (see paper)
39% identity, 32% coverage: 59:122/198 of query aligns to 615:683/753 of 1amjA

query
sites
1amjA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

S

S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

F

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: S641 (= S80), R643 (= R82)
binding sulfate ion: R643 (= R82)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding iron/sulfur cluster: 144, 166, 357, 420, 423
binding sulfate ion: 71, 579

1amiA Steric and conformational features of the aconitase mechanism (see paper)
39% identity, 32% coverage: 59:122/198 of query aligns to 615:683/753 of 1amiA

query
sites
1amiA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

F

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: S641 (= S80), R643 (= R82)
binding alpha-methylisocitric acid: S641 (= S80), S642 (= S81), R643 (= R82)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding alpha-methylisocitric acid: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 144, 164, 166, 356, 357, 420, 423, 445

1acoA Crystal structure of aconitase with transaconitate bound (see paper)
39% identity, 32% coverage: 59:122/198 of query aligns to 615:683/753 of 1acoA

query
sites
1acoA

V

V

L

P

N

D

N

T

S

A

R

R

Y

Y

K

Y

G

K

Q

Q

-

H

-

G

-

I

-

R

-

W

V

V

L

V

L

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

A

A

W

L

A

E

I

P

Y

R

D

F

A

L

G

G

F

G

R

R

V

A

V

I

I

I

S

T

S

K

Y

S

F

F

A

A

D

R

I

I

F

H

R

E

G

T

N

N

A

L

L

K

N

K

N

Q

G

G

L

L

P

P

L

L

Q

T

V

F

T

A

D

D

active site: S641 (= S80), R643 (= R82)
binding aconitate ion: S641 (= S80), S642 (= S81), R643 (= R82)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding iron/sulfur cluster: 100, 144, 164, 166, 356, 357, 420, 423, 445
binding aconitate ion: 71, 164, 165, 446, 451, 579

3snpA Crystal structure analysis of iron regulatory protein 1 in complex with ferritin h ire RNA (see paper)
37% identity, 26% coverage: 69:119/198 of query aligns to 728:778/850 of 3snpA

query
sites
3snpA

V

I

L

V

L

L

A

A

G

G

K

K

N

E

F

Y

G

G

C

S

G

G

S
|
S

S

S

R
|
R

E

D

H

W

A

A

W

K

A

G

I

P

Y

F

D

L

A

L

G

G

F

I

R

K

V

A

V

V

I

L

S

A

S

E

Y

S

F

Y

A

E

D

R

I

I

F

H

R

R

G

S

N

N

A

L

L

V

N

G

N

M

G

G

L

V

L

I

P

P

L

L

Q

E

active site: S739 (= S80), R741 (= R82)
binding : R741 (= R82)

Sites not aligning to the query:

active site: 124, 125, 186, 505
binding : 125, 126, 188, 243, 250, 279, 283, 352, 357, 360, 419, 420, 421, 504, 505, 520, 642, 643, 644, 645, 646, 649, 665, 669, 671, 674

2b3xA Structure of an orthorhombic crystal form of human cytosolic aconitase (irp1) (see paper)
37% identity, 26% coverage: 69:119/198 of query aligns to 766:816/888 of 2b3xA

query
sites
2b3xA

V

I

L

V

L

L

A

A

G

G

K

K

N

E

F

Y

G

G

C

A

G

G

S
|
S

S

S

R
|
R

E

D

H

W

A

A

W

K

A

G

I

P

Y

F

D

L

A

L

G

G

F

I

R

K

V

A

V

V

I

L

S

A

S

E

Y

S

F

Y

A

E

D

R

I

I

F

H

R

R

G

S

N

N

A

L

L

V

N

G

N

M

G

G

L

V

L

I

P

P

L

L

Q

E

active site: S777 (= S80), R779 (= R82)

Sites not aligning to the query:

active site: 124, 125, 204, 535
binding iron/sulfur cluster: 175, 206, 436, 502, 505, 506, 534

P21399 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Homo sapiens (Human) (see 2 papers)
37% identity, 26% coverage: 69:119/198 of query aligns to 767:817/889 of P21399

query
sites
P21399

V

I

L

V

L

L

A

A

G

G

K

K

N

E

F

Y

G

G

C

A

G

G

S
|
S

S

S

R
|
R

E

D

H

W

A

A

W

K

A

G

I

P

Y

F

D

L

A

L

G

G

F

I

R

K

V

A

V

V

I

L

S

A

S

E

Y

S

F

Y

A

E

D

R

I

I

F

H

R

R

G

S

N

N

A

L

L

V

N

G

N

M

G

G

L

V

L

I

P

P

L

L

Q

E

S778 (= S80) mutation to A: No effect on iron-regulated RNA binding. Loss of aconitase activity.
R780 (= R82) mutation to Q: Nearly abolishes RNA binding.

Sites not aligning to the query:

300 C→S: No effect on aconitase activity or on RNA binding.
318 T → M: in dbSNP:rs150373174
437 C→S: Loss of aconitase activity. Leads to constitutive RNA binding, irrespective of iron levels.
503 C→S: Loss of aconitase activity. Leads to constitutive RNA binding, irrespective of iron levels.
506 C→S: Loss of aconitase activity. Leads to constitutive RNA binding, irrespective of iron levels.
536 R→Q: Strongly reduced RNA binding.
541 R→Q: Strongly reduced RNA binding.
699 R→K: No effect on RNA binding.

Query Sequence

>WP_101445098.1 NCBI__GCF_002846395.1:WP_101445098.1
MEKFEIIRTGAVPLPIENIDTDQIIPARFLKATSREGFGENLFRDWRYSSSGEPKRDFVL
NNSRYKGQVLLAGKNFGCGSSREHAAWAIYDAGFRVVISSYFADIFRGNALNNGLLPLQV
TDEVLQRLFALVEADPQTEFVINLPEQELYLPASGERIPFAIDPYKKECLIKGYDDIDFL
VSQKEAITAYESTRAWIY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory