SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_101446326.1 NCBI__GCF_002846395.1:WP_101446326.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
27% identity, 51% coverage: 31:508/941 of query aligns to 39:460/467 of 7qh2C

query
sites
7qh2C

G

G

F

S

Y

I

Y

H

L

S

R

Y

P

P

K

E

A

V

V

L

V

I

Q

K

P

V

V

T

S

S

E

T

E

E

I

V

K

S

Q

K

L

I

F

M

A

K

F

Y

S

A

H

Y

Q

E

H

H

K

N

V

I

P

P

L

V

T

V

F
x
V

R

R

T
x
G

A
x
S

G
|
G

T
|
T

S
x
G

L
|
L

S

V

G

G

Q
x
A

S
x
C

I

V

T

P

-

L

-

F

D

G

G

G

I

I

L

M

V

L

D

E

L

T

S

T

Q

L

Y

M

W

N

N

N

K

I

V

L

L

E

V

L

E

D

L

T

E

E

G

N

A

L

Q

T

V

V

R

T

V

V

Q

E

P

P

G

G

V

V

I

L

G

L

A

M

I

E

V

L

N

S

A

K

H

F

L

V

R

E

P

E

Y

N

M

D

R

L

K

F

I

Y

G

P

P

P

D

D

P
|
P

A
x
G

S
x
E

I

-

N

K

S

S

A
|
A

M
x
T

M

I

G

A

G
|
G

I
x
N

L

I

S
|
S

N
x
T

N

N

A
|
A

S
x
G

G

G

M

M

C

R

C

A

-

V

-

K

-

Y

G

G

V

V

S

T

K

R

N

D

S

-

Y

-

H

-

T

-

K

-

Y

Y

I

V

R

R

-

G

-

L

-

T

F

V

V

V

L

L

P

A

N

N

G

G

L

-

S

-

Y

-

T

-

E

-

K

-

Q

-

I

-

D

-

Y

-

L

-

R

-

F

-

S

-

E

-

E

E

C

I

P

I

E

E

L

L

Y

G

L

G

T

K

L

I

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

-

H

-

Y

-

L

-

I

-

R

-

H

-

K

-

Y

-

K

-

T

V

K

K

N

N

T

S

V

S

G

G

Y

Y

S

S

V

-

N

-

A

-

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

L

A

K

H

D

L

L

L

V

I

I

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

C

F

V

I
|
I

A

T

E

K

A

A

V

I

L

L

Q

K

T

L

V

L

P

P

D

-

Y

L

P

P

S

K

K

M

S

T

T

L

A

S

L

L

Y

I

-

P

F

F

P

E

D

N

I

I

Y

S

A

D

A

A

C

A

Q

-

A

G

I

I

V

V

P

P

L

K

-

I

-

K

T

S

E

K

A

A

G

I

A

P

E

T

A

A

V

I

E

E

L

F

M

M

D

E

R

R

A

Q

S

T

L

I

R

L

S

F

I

A

E

E

H

D

M

F

A

L

G

G

V

-

P

-

A

-

V

-

I

-

K

K

T

K

L

F

P

P

E

D

T

S

A

S

-

S

-

N

A

A

A

Y

L

I

L

L

V

L

E

T

F

F

Q

D

A

G

N

N

T

T

P

K

E

E

E

Q

V

V

Q

E

Q

A

K

E

I

Y

L

E

S

T

F

V

M

A

A

N

L

L

A

C

P

-

D

-

L

L

A

A

L

E

L

G

E

A

Q

K

P

D

V

V

F

Y

T

I

T

V

D

D

P

T

I

V

E

E

Q

R

A

K

-

D

F

S

L

V

W

W

K

S

V

A

R

R

K

-

G

G

M

A

F

F

P

-

A

-

V

L

G

E

A

A

V

I

R

K

A

A

S

S

G

T

S

T

T

E

V

M

L

D

L

E

E

C

D

D

I

V

A

V

V

V

P

P

V

R

A

N

Q

R

L

I

G

A

D

E

A

F

I

I

L

E

D

F

L

T

Q

H

E

D

L

L

F

A

R

K

K

E

H

M

A

D

Y

V

H

R

N

I

A

P

I

S

I

-

F

F

G

G

H
|
H

A

A

K

G

D

D

G

G

N

N

I

L

H
|
H

F

I

V

Y

V

V

T

C

Q

R

A

-

F

-

D

-

T

-

P

-

D

D

E

E

V

L

-

C

-

Q

A

A

R

D

Y

W

D

E

R

A

F

K

L

L

K

A

E

E

V

A

V

M

K

D

L

R

V

M

V

Y

K

A

K

K

-

A

-

L

-

T

Y

F

E

E

G

G

A

L

L

V

K

S

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

Y

N

A

M

K

A

R

P

K

F

Y

V

L

E

L

T

N

E

D

W

F

G

G

P

T

E

E

V

H

Y

L

R

A

I

L

M

M

K

A

T

G

L

I

K

K

Q

Q

V

T

V

F

D

D

P

P

E

K

N

N

L

L

N

N

P

P

binding flavin-adenine dinucleotide: V73 (≠ F65), G75 (≠ T67), S76 (≠ A68), G77 (= G69), T78 (= T70), G79 (≠ S71), L80 (= L72), A83 (≠ Q75), C84 (≠ S76), P137 (= P127), G138 (≠ A128), E139 (≠ S129), A142 (= A133), T143 (≠ M134), G146 (= G137), N147 (≠ I138), S149 (= S140), T150 (≠ N141), A152 (= A143), G153 (≠ S144), E203 (= E245), G204 (= G246), I209 (= I251), E422 (= E470), H423 (= H471)
binding fe (iii) ion: H377 (= H425), H384 (= H432), E422 (= E470)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:455/455 of 8jduA

query
sites
8jduA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

H

R

M

H

A

S

G

K

V

L

P

N

A

C

V

P

I

V

K

-

T

-

L

-

P

-

E

-

T

-

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

-

P

-

E

-

V

-

Q

Q

K

T

I

L

L

A

S

E

F

Q

M

L

A

Q

L

R

A

T

P

E

D

A

L

I

A

T

L

Q

L

N

E

G

Q

G

P

S

V

H

F

F

T

S

T

W

D

A

P

A

I

E

E

K

Q

R

A

N

F

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

V

A

D

F

P

D

D

T

D

P

A

D

E

E

E

V

Q

A

R

R

R

Y

V

D

K

R

A

F

F

L

A

K

E

E

N

V

L

V

G

K

R

L

R

V

A

V

L

K

A

K

-

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding 2-oxopentanoic acid: R318 (= R372), W322 (≠ F376), H369 (= H425), H376 (= H432), H414 (= H471)
binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W322 (≠ F376), E413 (= E470), N450 (= N507)
binding manganese (ii) ion: H369 (= H425), H376 (= H432), E413 (= E470)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:455/455 of 8jdxA

query
sites
8jdxA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

-

R

H

H

M

S

A

L

G

N

V

C

P

P

A

-

V

-

I

-

K

-

T

-

L

-

P

-

E

-

T

V

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

Q

P

-

E

Q

E

T

V

L

Q

A

Q

E

K

Q

I

L

L

Q

S

R

F

T

M

E

A

A

L

I

A

T

P

Q

D

D

L

N

A

G

L

G

L

S

E

H

Q

-

P

-

V

-

F

F

T

S

T

W

D

A

P

A

I

E

E

K

Q

R

A

N

F

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

V

A

D

F

P

D

D

T

D

P

A

D

E

E

E

V

Q

A

R

R

R

Y

V

D

K

R

A

F

F

L

A

K

E

E

N

V

L

V

G

K

R

L

R

V

A

V

L

K

A

K

-

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W322 (≠ F376), E413 (= E470), H414 (= H471), N450 (= N507)
binding 3-methyl-2-oxobutanoic acid: R318 (= R372), H369 (= H425), H376 (= H432), H414 (= H471)
binding manganese (ii) ion: H369 (= H425), H376 (= H432), E413 (= E470)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:456/456 of 8jdsA

query
sites
8jdsA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

H

R

M

H

A

S

G

K

V

L

P

N

A

C

V

P

I

V

K

-

T

-

L

-

P

-

E

-

T

-

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

Q

P

-

E

Q

E

T

V

L

Q

A

Q

E

K

Q

I

L

L

Q

S

R

F

T

M

E

A

A

L

I

A

T

P

Q

D

D

L

N

A

G

L

G

L

S

E

H

Q

-

P

-

V

-

F

F

T

S

T

W

D

A

P

A

I

E

E

K

Q

R

A

N

F

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

V

A

D

F

P

D

D

T

D

P

A

D

E

E

E

V

Q

A

R

R

R

Y

V

D

K

R

A

F

F

L

A

K

E

E

N

V

L

V

G

K

R

L

R

V

A

V

L

K

A

K

-

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W323 (≠ F376), E414 (= E470), H415 (= H471), N451 (= N507)
binding manganese (ii) ion: H370 (= H425), H377 (= H432), E414 (= E470)
binding pyruvic acid: R319 (= R372), H370 (= H425), H377 (= H432), H415 (= H471)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:455/455 of 8jdeA

query
sites
8jdeA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

H

R

M

H

A

S

G

K

V

L

P

N

A

C

V

P

I

V

K

-

T

-

L

-

P

-

E

-

T

-

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

-

P

-

E

-

V

-

Q

Q

K

T

I

L

L

A

S

E

F

Q

M

L

A

Q

L

R

A

T

P

E

D

A

L

I

A

T

L

Q

L

D

E

N

Q

G

P

G

V

S

F

H

T

F

T

S

D

W

P

A

I

A

E

E

Q

K

A

N

F

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

V

A

D

F

P

D

D

T

D

P

A

D

E

E

E

V

Q

A

R

R

R

Y

V

D

K

R

A

F

F

L

A

K

E

E

N

V

L

V

G

K

R

L

R

V

A

V

L

K

A

K

-

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W322 (≠ F376), E413 (= E470), H414 (= H471), N450 (= N507)
binding lactic acid: R318 (= R372), H369 (= H425), H376 (= H432), H414 (= H471)
binding manganese (ii) ion: H369 (= H425), H376 (= H432), E413 (= E470)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:454/454 of 8jdvA

query
sites
8jdvA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T
x
V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

H

R

M

H

A

S

G

K

V

L

P

N

A

C

V

P

I

V

K

-

T

-

L

-

P

-

E

-

T

-

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

Q

P

-

E

Q

E

T

V

L

Q

A

Q

E

K

Q

I

L

L

Q

S

R

F

T

M

E

A

A

L

I

A

T

P

Q

D

D

L

N

A

G

L

G

L

S

E

H

Q

-

P

-

V

-

F

F

T

S

T

A

D

E

P

K

I

R

E

N

Q

E

A

-

F

-

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P
x
Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

V

A

D

F

P

D

D

T

D

P

A

D

E

E

E

V

Q

A

R

R

R

Y

V

D

K

R

A

F

F

L

A

K

E

E

N

V

L

V

G

K

R

L

R

V

A

V

L

K

A

K

-

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding 2-Ketohexanoic acid: V75 (≠ T70), R317 (= R372), W321 (≠ F376), H368 (= H425), H375 (= H432), H413 (= H471)
binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W321 (≠ F376), Y322 (≠ P377), E412 (= E470), H413 (= H471), N449 (= N507)
binding manganese (ii) ion: H368 (= H425), H375 (= H432), E412 (= E470)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
23% identity, 51% coverage: 37:512/941 of query aligns to 40:455/455 of 8jdtA

query
sites
8jdtA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

H

R

M

H

A

S

G

K

V

L

P

N

A

C

V

P

I

V

K

-

T

-

L

-

P

-

E

-

T

-

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

-

T

-

P

-

E

-

V

-

Q

-

K

-

I

-

L

-

S

S

F

Q

M

Q

A

T

L

L

A

A

P

E

D

Q

L

L

A

Q

L

R

L

T

E

E

Q

-

P

-

V

A

F

I

T

T

T

Q

D

D

P

N

I

G

E

G

Q

S

A

H

F

F

-

S

-

W

-

A

-

E

-

K

-

R

-

N

-

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

V

A

D

F

P

D

D

T

D

P

A

D

E

E

E

V

Q

A

R

R

R

Y

V

D

K

R

A

F

F

L

A

K

E

E

N

V

L

V

G

K

R

L

R

V

A

V

L

K

-

K

A

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding 2-ketobutyric acid: R318 (= R372), H369 (= H425), H376 (= H432), H414 (= H471)
binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W322 (≠ F376), E413 (= E470), H414 (= H471), N450 (= N507)
binding manganese (ii) ion: H369 (= H425), H376 (= H432), E413 (= E470)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:454/454 of 8jdzA

query
sites
8jdzA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

-

R

H

H

M

S

A

L

G

N

V

C

P

P

A

-

V

-

I

-

K

-

T

-

L

-

P

-

E

-

T

V

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

Q

P

-

E

Q

E

T

V

L

Q

A

Q

E

K

Q

I

L

L

Q

S

R

F

T

M

E

A

A

L

I

A

T

P

Q

D

D

L

N

A

G

L

G

L

S

E

H

Q

-

P

-

V

-

F

F

T

S

T

W

D

A

P

A

I

E

E

K

Q

R

A

N

F

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L

S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

-

A

-

F

V

D

D

T

P

P

D

D

A

E

E

V

E

A

Q

R

R

Y

R

D

V

R

K

F

A

L

F

K

A

E

E

V

N

V

L

K

G

L

R

V

R

V

A

K

L

K

A

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R372), W322 (≠ F376), H369 (= H425), H376 (= H432), H413 (= H471)
binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F65), T71 (≠ R66), G72 (≠ T67), T73 (≠ A68), G74 (= G69), G78 (≠ S73), V79 (≠ G74), L90 (= L85), P132 (= P127), G133 (≠ A128), A134 (≠ S129), G140 (= G137), M141 (≠ I138), A143 (≠ S140), T144 (≠ N141), A146 (= A143), S147 (= S144), E200 (= E245), G201 (= G246), I206 (= I251), W322 (≠ F376), E412 (= E470), H413 (= H471), N449 (= N507)
binding manganese (ii) ion: H369 (= H425), H376 (= H432), E412 (= E470)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
22% identity, 51% coverage: 37:512/941 of query aligns to 40:454/454 of 8jdyA

query
sites
8jdyA

P

P

K

D

A

A

V

V

Q

W

P

P

V

Q

S

N

E

V

E

D

E

Q

I

V

K

S

Q

R

L

V

F

A

A

S

F

L

S

C

H

Y

Q

N

H

Q

K

G

V

V

P

P

L

I

T

I

-
x
P

-

F

F
x
G

R
x
T

T
x
G

A
x
T

G
|
G

T

V

S

E

L

G

S
x
G

G
x
V

Q

C

S

A

I

V

T

Q

D

G

G

G

I

V

L

C

V

I

D

N

L
|
L

S

T

Q

H

Y

M

W

D

N

Q

K

I

V

T

L

E

V

L

E

N

L

T

E

E

G

D

A

F

Q

S

V

V

R

V

V

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

I

A

V

L

N

N

A

T

H

H

L

L

R

R

P

D

Y

S

M

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

-

N

-

S

D

A

A

M

S

M

L

G

C

G
|
G

I
x
M

L

A

S
x
A

N
x
T

N

G

A
|
A

S
|
S

G

G

M

T

C

-

G

-

V

-

S

-

K

-

N

-

S

-

Y

-

H

-

T

-

K

-

Y

-

I

-

R

-

F

-

V

-

L

-

P

-

N

N

G

A

L

V

S

R

Y

Y

T

G

T

T

E

M

K

R

Q

D

I

-

D

N

Y

V

L

I

R

N

F

L

S

E

E

V

C

L

P

P

E

D

L

G

Y

R

L

L

T

-

L

-

Q

-

K

-

I

-

K

-

Q

-

V

-

R

-

N

-

P

-

E

-

L

L

H

H

Y

T

L

A

I

G

R

R

H

G

K

R

Y

H

K

Y

T

R

K

K

N

S

T

A

V

A

G

G

Y

Y

S

N

V

L

N

T

A

G

F

-

I

-

D

-

Y

-

E

-

H

-

P

-

L

-

D

-

I

-

M

-

A

-

H

-

L

L

L

F

I

V

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

A

T

E

S

A

T

V

T

L

L

Q

R

T

L

V

H

P

P

D

A

Y

P

P

E

S

A

K

T

S

V

T

A

A

A

L

T

L

C

Y

A

F

F

P

P

D

S

I

V

Y

Q

A

A

C

V

Q

D

A

S

I

T

V

V

P

Q

L

I

T

L

E

Q

A

A

G

A

A

V

E

P

A

V

-

A

-

R

V

I

E

E

L

F

M

L

D

D

R

D

A

V

S

M

L

M

R

D

S

A

I

C

E

N

-

R

H

H

M

S

A

L

G

N

V

C

P

P

A

-

V

-

I

-

K

-

T

-

L

-

P

-

E

-

T

V

A

A

A

P

A

T

L

L

L

F

V

L

E

E

F

F

Q

H

A

G

N

S

T

Q

P

-

E

Q

E

T

V

L

Q

A

Q

E

K

Q

I

L

L

Q

S

R

F

T

M

E

A

A

L

I

A

T

P

Q

D

D

L

N

A

G

L

G

L

S

E

H

Q

-

P

-

V

-

F

F

T

S

T

W

D

A

P

A

I

E

E

K

Q

R

A

N

F

E

L

L

W

W

K

A

V

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

A

S

S

P

G

G

S

S

T

K

V

A

L

Y

L
x
S

E

T

D

D

I

V

A

C

V

V

P

P

V

I

A

S

Q

R

L

L

G

P

D

E

A

I

I

L

L

V

D

E

L

T

Q

K

E

E

L

E

F

I

R

K

K

A

H

S

A

K

Y

L

H

T

N

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

K

G

D

D

G

G

N

N

I

F

H
|
H

F

C

V

I

V

L

T

L

Q

-

A

-

F

V

D

D

T

P

P

D

D

A

E

E

V

E

A

Q

R

R

Y

R

D

V

R

K

F

A

L

F

K

A

E

E

V

N

V

L

K

G

L

R

V

R

V

A

K

L

K

A

Y

L

E

G

G

G

A

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

F

L

V

L

E

Q

T

E

E

E

W

V

G

G

P

P

E

V

V

G

Y

V

R

E

I

T

M

M

K

R

T

Q

L

L

K

K

Q

N

V

T

V

L

D

D

P

P

E

R

N

G

L

L

L

M

N
|
N

P

P

G

G

V

K

I

V

I

L

binding 2-oxo-4-methylpentanoic acid: