SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_101586818.1 NCBI__GCF_900169175.1:WP_101586818.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

6r6uA Crystal structure of human cis-aconitate decarboxylase (see paper)
23% identity, 81% coverage: 87:488/498 of query aligns to 70:457/458 of 6r6uA

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binding (r,r)-2,3-butanediol: A148 (vs. gap), F155 (≠ E163), F242 (≠ W255), Y243 (≠ I256)

A6NK06 Cis-aconitate decarboxylase; CAD; Aconitate decarboxylase; Aconitate decarboxylase 1; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; EC 4.1.1.6 from Homo sapiens (Human) (see paper)
24% identity, 81% coverage: 87:488/498 of query aligns to 73:460/481 of A6NK06

query
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D93 (= D107) mutation to A: Abolished cis-aconitate decarboxylase activity.
T97 (= T111) to M: strongly reduced cis-aconitate decarboxylase activity; dbSNP:rs767323284; mutation to A: Strongly reduced cis-aconitate decarboxylase activity.
H103 (= H119) mutation to A: Abolished cis-aconitate decarboxylase activity.
N152 (≠ K157) to S: increased cis-aconitate decarboxylase activity
H159 (= H164) to Q: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; to R: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1471882722; mutation to A: Abolished cis-aconitate decarboxylase activity.
K207 (≠ W216) mutation to A: Abolished cis-aconitate decarboxylase activity.
K272 (= K284) to Q: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1018207074; mutation to A: Abolished cis-aconitate decarboxylase activity.
R273 (≠ E285) to H: does not affect cis-aconitate decarboxylase activity
H277 (≠ E289) to Y: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1289708092; mutation to A: Abolished cis-aconitate decarboxylase activity.
Y318 (= Y325) mutation to A: Abolished cis-aconitate decarboxylase activity.
R331 (= R343) to H: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs755737247

Query Sequence

>WP_101586818.1 NCBI__GCF_900169175.1:WP_101586818.1
MINHEVRTYKSSESLAREDQLAWKIAEVASDDVAVETDVQDMVINRVIDNAAVAAASVRR
GAPVHAREQAQGHPMKPGASVFGLSLDETISPEWAAWANGVAVRELDFHDTFLAKEYSHP
GDNIPAVLAVAQHKGISGADVVRGLATGYEIQVDLVKGICLHEHKIDHVAHLGPSAAAGI
GTLLNLPTEVIYQAVQQALHVTTATRQSRKGEISTWKAHAPAFAGKMAVEAVDRAMRGEG
APSPIYEGEDGFIAWILSGPEARYTVPLPGTGEAKRAIMDTYTKEHSAEYQAQALIDLAR
RMGRQIDDFSTIRRVTIHTSHHTHYVIGTGANDPQKMDPKASRETLDHSIMYMFTVALED
QAWHHEKSYAPERAGRPETVELWHKVETREDPEWTRRYHSTDPDEMAFGGRVEIEFEDGT
TQVDEIAVADAHPNGARPFVRENYVNKFRTLAEGIVSEQEQNRFLDLAENLASLGSDDVR
ALGFAVEGLEHSGSKGIF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory