SitesBLAST
Comparing WP_101588590.1 NCBI__GCF_900169175.1:WP_101588590.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 97% coverage: 9:265/265 of query aligns to 4:257/257 of 6slbAAA
- active site: Q64 (≠ A69), F69 (≠ K74), L80 (= L88), N84 (= N92), A108 (≠ V116), S111 (= S119), A130 (= A138), F131 (= F139), L136 (= L144), P138 (= P146), D139 (= D147), A224 (≠ N232), G234 (≠ L242)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R63), A62 (≠ S67), Q64 (≠ A69), D65 (= D70), L66 (= L71), Y76 (≠ G81), A108 (≠ V116), F131 (= F139), D139 (= D147)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 97% coverage: 9:265/265 of query aligns to 1:245/245 of 6slaAAA
- active site: Q61 (≠ A69), L68 (= L88), N72 (= N92), A96 (≠ V116), S99 (= S119), A118 (= A138), F119 (= F139), L124 (= L144), P126 (= P146), N127 (≠ D147), A212 (≠ N232), G222 (≠ L242)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L29), A59 (≠ S67), Q61 (≠ A69), D62 (= D70), L63 (= L71), L68 (= L88), Y71 (≠ V91), A94 (= A114), G95 (= G115), A96 (≠ V116), F119 (= F139), I122 (= I142), L124 (= L144), N127 (≠ D147), F234 (= F254), K237 (= K257)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
29% identity, 96% coverage: 12:265/265 of query aligns to 8:259/259 of 5zaiC
- active site: A65 (= A69), F70 (vs. gap), S82 (≠ G81), R86 (≠ G85), G110 (≠ V116), E113 (≠ S119), P132 (≠ A138), E133 (≠ F139), I138 (≠ L144), P140 (= P146), G141 (≠ D147), A226 (≠ N232), F236 (≠ L242)
- binding coenzyme a: K24 (≠ R28), L25 (= L29), A63 (≠ S67), G64 (= G68), A65 (= A69), D66 (= D70), I67 (≠ L71), P132 (≠ A138), R166 (= R172), F248 (= F254), K251 (= K257)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
34% identity, 96% coverage: 12:265/265 of query aligns to 8:261/261 of 5jbxB
- active site: A67 (= A69), R72 (≠ K74), L84 (= L88), R88 (≠ N92), G112 (≠ V116), E115 (≠ S119), T134 (≠ A138), E135 (≠ F139), I140 (≠ L144), P142 (= P146), G143 (≠ D147), A228 (≠ N232), L238 (= L242)
- binding coenzyme a: S24 (≠ H27), R25 (= R28), R26 (≠ L29), A28 (= A31), A65 (≠ S67), D68 (= D70), L69 (= L71), K70 (≠ Q72), L110 (≠ A114), G111 (= G115), T134 (≠ A138), E135 (≠ F139), L138 (≠ I142), R168 (= R172)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 95% coverage: 11:262/265 of query aligns to 4:255/258 of 1ey3A
- active site: A66 (= A69), M71 (≠ K74), S81 (= S84), L85 (= L88), G109 (≠ V116), E112 (≠ S119), P131 (≠ A138), E132 (≠ F139), T137 (≠ L144), P139 (= P146), G140 (≠ D147), K225 (≠ N232), F235 (≠ L242)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ H27), L26 (= L29), A28 (= A31), A64 (≠ S67), G65 (= G68), A66 (= A69), D67 (= D70), I68 (≠ L71), L85 (= L88), W88 (≠ V91), G109 (≠ V116), P131 (≠ A138), L135 (≠ I142), G140 (≠ D147)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 95% coverage: 11:262/265 of query aligns to 6:257/260 of 1dubA
- active site: A68 (= A69), M73 (≠ K74), S83 (= S84), L87 (= L88), G111 (≠ V116), E114 (≠ S119), P133 (≠ A138), E134 (≠ F139), T139 (≠ L144), P141 (= P146), G142 (≠ D147), K227 (≠ N232), F237 (≠ L242)
- binding acetoacetyl-coenzyme a: K26 (≠ H27), A27 (≠ R28), L28 (= L29), A30 (= A31), A66 (≠ S67), A68 (= A69), D69 (= D70), I70 (≠ L71), Y107 (≠ A112), G110 (= G115), G111 (≠ V116), E114 (≠ S119), P133 (≠ A138), E134 (≠ F139), L137 (≠ I142), G142 (≠ D147), F233 (≠ Q238), F249 (= F254)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
30% identity, 95% coverage: 11:262/265 of query aligns to 36:287/290 of P14604
- E144 (≠ S119) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ F139) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
31% identity, 91% coverage: 21:262/265 of query aligns to 20:257/260 of 2hw5C
- active site: A68 (= A69), M73 (≠ K74), S83 (= S84), L87 (= L88), G111 (≠ V116), E114 (≠ S119), P133 (≠ A138), E134 (≠ F139), T139 (≠ L144), P141 (= P146), G142 (≠ D147), K227 (≠ D230), F237 (≠ L242)
- binding crotonyl coenzyme a: K26 (≠ H27), A27 (≠ R28), L28 (= L29), A30 (= A31), K62 (≠ R63), I70 (≠ L71), F109 (≠ A114)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 95% coverage: 11:262/265 of query aligns to 6:255/258 of 1mj3A
- active site: A68 (= A69), M73 (≠ K74), S83 (= S84), L85 (vs. gap), G109 (≠ V116), E112 (≠ S119), P131 (≠ A138), E132 (≠ F139), T137 (≠ L144), P139 (= P146), G140 (≠ D147), K225 (≠ N232), F235 (≠ L242)
- binding hexanoyl-coenzyme a: K26 (≠ H27), A27 (≠ R28), L28 (= L29), A30 (= A31), A66 (≠ S67), G67 (= G68), A68 (= A69), D69 (= D70), I70 (≠ L71), G109 (≠ V116), P131 (≠ A138), E132 (≠ F139), L135 (≠ I142), G140 (≠ D147)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
31% identity, 97% coverage: 9:265/265 of query aligns to 5:256/256 of 3h81A
- active site: A64 (= A69), M69 (≠ K74), T79 (≠ S84), F83 (≠ L88), G107 (≠ V116), E110 (≠ S119), P129 (≠ A138), E130 (≠ F139), V135 (≠ L144), P137 (= P146), G138 (≠ D147), L223 (≠ N232), F233 (≠ L242)
- binding calcium ion: F233 (≠ L242), Q238 (≠ A247)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 95% coverage: 11:262/265 of query aligns to 5:251/254 of 2dubA
- active site: A67 (= A69), M72 (≠ E76), S82 (= S86), G105 (≠ V116), E108 (≠ S119), P127 (≠ A138), E128 (≠ F139), T133 (≠ L144), P135 (= P146), G136 (≠ D147), K221 (≠ N232), F231 (≠ L242)
- binding octanoyl-coenzyme a: K25 (≠ H27), A26 (≠ R28), L27 (= L29), A29 (= A31), A65 (≠ S67), A67 (= A69), D68 (= D70), I69 (≠ L71), K70 (= K74), G105 (≠ V116), E108 (≠ S119), P127 (≠ A138), E128 (≠ F139), G136 (≠ D147), A137 (≠ G148)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
31% identity, 96% coverage: 9:263/265 of query aligns to 6:255/255 of 3q0jC
- active site: A65 (= A69), M70 (≠ K74), T80 (≠ S84), F84 (≠ L88), G108 (≠ V116), E111 (≠ S119), P130 (≠ A138), E131 (≠ F139), V136 (≠ L144), P138 (= P146), G139 (≠ D147), L224 (≠ N232), F234 (≠ L242)
- binding acetoacetyl-coenzyme a: Q23 (≠ H27), A24 (≠ R28), L25 (= L29), A27 (= A31), A63 (≠ S67), G64 (= G68), A65 (= A69), D66 (= D70), I67 (≠ L71), K68 (≠ Q72), M70 (≠ K74), F84 (≠ L88), G107 (= G115), G108 (≠ V116), E111 (≠ S119), P130 (≠ A138), E131 (≠ F139), P138 (= P146), G139 (≠ D147), M140 (≠ G148)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 96% coverage: 9:263/265 of query aligns to 6:255/255 of 3q0gC
- active site: A65 (= A69), M70 (≠ K74), T80 (≠ S84), F84 (≠ L88), G108 (≠ V116), E111 (≠ S119), P130 (≠ A138), E131 (≠ F139), V136 (≠ L144), P138 (= P146), G139 (≠ D147), L224 (≠ N232), F234 (≠ L242)
- binding coenzyme a: L25 (= L29), A63 (≠ S67), I67 (≠ L71), K68 (≠ Q72), Y104 (≠ A112), P130 (≠ A138), E131 (≠ F139), L134 (≠ I142)
5ducA Crystal structure of m. Tuberculosis echa6 bound to ligand gsk951a (see paper)
32% identity, 95% coverage: 13:264/265 of query aligns to 6:243/244 of 5ducA
- active site: A61 (= A69), D66 (≠ K74), P73 (≠ L88), I77 (≠ N92), A101 (≠ V116), Q104 (≠ S119), P123 (≠ A138), T124 (≠ F139), L129 (= L144), L131 (≠ P146), D132 (= D147), P211 (= P225), W221 (≠ L242)
- binding (5R,7S)-N-(1,3-benzodioxol-5-ylmethyl)-5-(4-ethylphenyl)-7-(trifluoromethyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxamide: L76 (≠ V91), H80 (≠ I95), D84 (≠ K99), Q104 (≠ S119), D132 (= D147), W134 (≠ G149), F217 (≠ V236)
5du4A Crystal structure of m. Tuberculosis echa6 bound to ligand gsk366a (see paper)
32% identity, 95% coverage: 13:264/265 of query aligns to 6:243/244 of 5du4A
- active site: A61 (= A69), D66 (≠ K74), P73 (≠ L88), I77 (≠ N92), A101 (≠ V116), Q104 (≠ S119), P123 (≠ A138), T124 (≠ F139), L129 (= L144), L131 (≠ P146), D132 (= D147), P211 (= P225), W221 (≠ L242)
- binding (5R,7S)-5-(4-ethylphenyl)-N-(4-methoxybenzyl)-7-(trifluoromethyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxamide: L76 (≠ V91), I77 (≠ N92), H80 (≠ I95), D84 (≠ K99), Q104 (≠ S119), D132 (= D147), W134 (≠ G149)
5dtwA Crystal structure of m. Tuberculosis echa6 bound to c20-coa (see paper)
32% identity, 95% coverage: 13:264/265 of query aligns to 6:243/244 of 5dtwA